M. TECH. BIOTECHNOLOGY 1. The following is the recognition sequence of which restriction endonuclease ? G — G — A — T — C — C C — C — T — A — G — G (A) EcoR1 (B) Bam H1 (C) Pst1 (D) Sau2 A1 2. Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) is an effective method to separate which molecule in a population ? (A) DNA (B) RNA (C) Proteins (D) Carbohydrates 3. To convert mRNA to double stranded DNA, which of the following enzymes is used ? (A) DNA Polymerase I (B) RNA polymerase (C) Invertase (D) Reverse transcriptase 4. Which type of restriction endonucleases bind to and cut within a specific DNA sequence ? (A) Type I (B) Type II (C) Type III (D) All of these 5. A genomic library is the collection of which fragments each cloned into a vector that, that represents the entire genome ? (A) Proteins (B) Lipids (C) DNA (D) RNA M-CL-09(BT) 1 P.T.O. 6. Antibodies (immunoglobulins) are glycoproteins, that are activated by a specific antigen are synthesized by : (A) T-lymphocytes (B) B-lymphocytes (C) RBC (D) Heart 7. Which of the following is not a blood type ? (A) A (B) B (C) N (D) O 8. Which of the following diseases is caused by Agrobacterium tumefaciens ? (A) Crown gall (B) Hairy roots (C) Wilt (D) Necrosis 9. T-DNA region of transforming Ti plasmids contain genes for biosynthesis of : (A) Lipids (B) Phytochrome-C (C) Auxin and cytokinin (D) Photosystem - II 10. Which of the following is an example of type of cytokinin used in plant micro- propagation ? (A) 2, 4-D (B) NAA (C) BAP (D) IAA 11. The plant tissue culture technique used for production of haploid plants is : (A) Ovary culture (B) Shoot tip culture (C) Anther culture (D) Axillary bud culture M-CL-09(BT) 2 12. Which of the following transformation methods is preferred for producing transgenic mice ? (A) Biolistics (B) Agrobacterium mediated (C) Electroporation (D) DNA microinjection 13. Members of which genus are the most predominant group of soil microorganisms that degrade xenobiotic compounds ? (A) Pseudomonas (B) Saccharomyces (C) Agrobacterium (D) Bacillus 14. Starch is the major food reserve polysaccharide in : (A) Animals (B) Fungi (C) Bacteria (D) Plants 15. Which of the following is a photosynthetic microorganism ? (A) E.coli (B) B. subtilis (C) Cynobacteria (D) Zymomonas 16. In a fermenter, in which phase of cell growth, cells adopt to new environmental conditions ? (A) Lag phase (B) Log phase (C) Stationary phase (D) Death phase 17. In which type of fermentation process, substrate is added in increments at various times throughout the course of reaction ? (A) Batch fermentation (B) Fed-Batch fermentation (C) Continuous fermentation (D) All of these M-CL-09(BT) 3 P.T.O. 18. Which of the following is non-mechanical method of cell disruptim ? (A) Ultrasonication (B) Wet milling (C) Use of enzymes (D) Impingement 19. Which of the following is not a membrane based separation system ? (A) Ultrafiltration (B) Reverse osmosis (C) Sonication (D) Electrodialysis 20. The optimum temperature and pressure for sterilization of culture vessels is : (A) 150ºC and 25 psi (B) 121ºC and 15 psi (C) 150ºC and 15 psi (D) 121ºC and 121 psi 21. A protoplast is devoid of : (A) Cytoplasm (B) Nucleus (C) Cell wall (D) None of these 22. The chemical most widely used for DNA uptake in intact tissues is : (A) Polyethylene Glycol (B) Fe- EDTA (C) Tris buffer (D) None of these 23. Agar is extracted from : (A) Bacteria (B) Red algae (C) Fungi (D) Yeast 24. Yoghurt is produced by which of the following microorganisms ? (A) Streptococcus thermophilus (B) Lactobacillus bulgaricus (C) Lactobacillus delbrueckii (D) Both (A) and (B) M-CL-09(BT) 4 25. Cheese production involves which of the following steps ? I. Coagulum formation II. Separation of curd from whey III. Ripening of cheese Choose the correct options : (A) I, II (B) II, III (C) I, II, III (D) I, III 26. Ordinarily, saccharification during beer production is stopped by boiling the wort when about how much of the starch has been converted into fermentable sugars ? (A) 25% (B) 50% (C) 75% (D) 90% 27. Which of the following proteases is used for meat tenderization ? (A) Papain (B) Heat labile fungal protease (C) Subtilisin (D) Neutral or alkaline proteases 28. Which of the following organisms is used for SCP production shows the highest growth rate ? (A) Bacteria (B) Algae (C) Yeast (D) Filamentous fungi 29. Which of the following scientists is recognized as father of microbiology ? (A) Robert Koch (B) Edward Jenner (C) Louis Pasteur (D) Aristotle M-CL-09(BT) 5 P.T.O. 30. Aabomycin A shows which of the following activities ? (A) Antifungal (B) Antibacterial (C) Antiviral (D) Antiprotozoal 31. Among the given polymer matrices, which one is the most commonly used for cell immobilization ? (A) Polyglycol oligomers (B) Polyacrylamide gel (C) Calcium alginate (D) K-Carageenan 32. Which of the following is the most commonly used to achieve cell disruption ? (A) Drying (B) Lysis (C) Mechanical cell disruption (D) All of these 33. In case of downstream processing commonly used approach of drying is which of the following ? (A) Spray drying (B) Freeze drying (C) Vacuum drying (D) All of these 34. In case of downstream processing, separation of cells from liquid medium is achieved by which of the following ? (A) Centrifugation (B) Filtration (C) Flocculation and floating (D) All of the above M-CL-09(BT) 6 35. Which of the following options indicates the correct order of ascorbic acid production ? (A) Glucose ascorbic acid D-sorbitol L-sorbose (B) Glucose L-sorbose D-sorbitol ascorbic acid (C) Glucose D-sorbitol L-sorbose ascorbic acid (D) D-sorbitol L-sorbose glucose ascorbic acid 36. In which of the following, enzymes are used in the largest quantity ? (A) Textile industry (B) Alcohol production (C) Detergents (D) Leather industry 37. Which of the following enzymes catalyses an irreversible reaction of glycolysis ? (A) Phosphofructokinase (B) Glucose-6-Isomerase (C) Aldolase (D) Phosphoglycerate mutase 38. Lyase class of enzymes does not contain which of the following ? (A) Decarboxylases (B) Dehydratases (C) Aldolases (D) Proteases 39. Which of the following enzymes is not obtained commercially from malted barley ? (A) -Glucanase (B) -Amylase (C) Glucoamylase (D) -Amylase 40. Which of the following biosensor types has the lowest cost ? (A) Potentiometric (B) Conductimetric (C) Piezoelectric (D) Thermometric M-CL-09(BT) 7 P.T.O. 41. Which of the following is not an example of physical pollutants ? (A) Dust and mechanical stress (B) Sound pollution and electrical storms (C) Heavy metals (D) Radiation and fires 42. Among the given microorganisms, which one is the most commonly found in aerobic digestion systems ? (A) Fungi (B) Bacteria (C) Protozoa (D) Algae 43. Depletion of ozone layer is caused by which of the following ? – (A) CO2 (B) SO2 (C) CFC (D) Both (B) and (C) 44. Which of the following gases contributes the maximum to the ‘greenhouse effect’ ? (A) CO2 (B) CFC (C) CH4 (D) N2O 45. Which of the following greenhouse gases is being generated by agricultural fields ? (A) Sulphur dioxide (B) SO3 (C) Nitrous oxide (D) Ammonia 46. Which of the following is regarded as the third revolution in vaccines ? (A) Recombinant protein vaccines (B) Recombinant polypeptide vaccines (C) DNA vaccines (D) All of these M-CL-09(BT) 8 47. Which of the following cannot be used for treatment of AIDS ? (A) Interferon (B) Granulocyte macrophage colony stimulating factor (C) Streptokinase (D) Didanosine 48. Which of the following plays the major role in histocompatibility ? (A) MHC loci (B) ABO blood group (C) Rh blood group (D) MN blood group 49. Immuno-PCR detects the presence of which of the following ? (A) DNA (B) RNA (C) Antigen (D) Immunogen 50. At a VNTR locus, which of the following is the basis for different alleles ? (A) Sequence of repeating units (B) Number of repeating units (C) Length of repeating units (D) Both (A) and (C) 51. Chemolithotroophs are those bacteria which can utilize : (A) Inorganic material as the energy source (B) Light as the energy source (C) Organic compound as the electron source (D) Crude oil as carbon source M-CL-09(BT) 9 P.T.O. 52. All the members of the genus Bacillus are known for : (A) Their ability to break down sulphur containing compounds (B) Their ability to form spores (C) Their ability to live in the absence of oxygen (D) The capsules they possess 53. Autoclaves are routinely used in laboratories for sterilization. It acts by : (A) Disrupting cell membranes (B) Denaturing proteins (C) Changing physically membrane lipids (D) All of the above 54. Pasteurization of milk is done by : (A) Boiling the milk for 20 minutes (B) Heating the milk at 72ºC for 30 minutes (C) Heating the milk at 72ºC for 20 minutes (D) Heating the milk at 62ºC for 30 minutes 55. Interferon : (A) is species specific (B) reacts directly with virus particles to inactivate them (C) reacts with cells, and the affected cells then become resistant to a number of different viruses (D) is constitutively produced at high levels in cells but requires an inducer for activity M-CL-09(BT) 10 56. The group of bacteria that do not have cell walls are the : (A) Archeobacteria (B) Mycobacteria (C) Mycoplasma (D) Both (A) and (B) correct 57. The genome of organism includes genes from : (A) Chromosome (B) Mitochondria (C) Plasmids (D) All of these 58. A virus with RNA-dependent RNA polymerase : (A) Synthesizes DNA from an RNA template (B) Synthesizes dsRNA from an RNA template (C) Synthesizes dsRNA from a DNA template (D) Transcribes mRNA from DNA 59.