Prof. Andrew L. Miller Curriculum Vitae

Present Position : Professor Address: Phone: 852 2358 8631 Date of Birth: 21/05/58 Division of Life Science, Fax: 852 2358 1559 HKUST, E-mail: [email protected] Clear Water Bay, Kowloon, Hong Kong SAR

Education Ph.D.: "Electrical Control of Root Development", with Prof. J.A. Raven, FRS, FRSE, University of 1983-1986 Dundee, Dundee, Scotland, UK Britannia Royal Naval College, Dartmouth, South Devon, England, UK. Royal Navy Direct 1982-1983 Graduate University Entrant. Rank: Sub-Lieutenant 1978-1982 B.Sc. (Hons.) 1st Class, Botany, University of Dundee, Dundee, Scotland, UK

1976 -1978 Gordonstoun School, Morayshire, Scotland, UK

1970-1976 Anderson High School, Lerwick, Shetland Islands, Scotland, UK

Research and Professional Experience 2010-Present Professor Division of Life Science, HKUST 2006-2010 Professor Department of Biology, HKUST 2000-2006 Associate Professor Department of Biology, HKUST 1995-2000 Assistant Professor Department of Biology, HKUST 1992-1995 Principal Investigator Marine Biological Laboratory (MBL), Woods Hole, MA 02543, USA. 1991-1995 Assistant Scientist MBL, Woods Hole, MA 02543, USA 1988-1991 Post Doctoral position In the laboratory of Dr. Lionel F. Jaffe at the MBL, Woods Hole, MA 02543, USA 1986-1988 Post Doctoral position In the laboratory of Prof. Neil Gow, FRSE, in the Department of Cell and Molecular Biology, Marischal College, University of Aberdeen, Scotland, UK

Adjunct Appointments 2011-Present Adjunct Senior MBL, Woods Hole, MA 02543, USA Scientist

Research Interests

Investigating the role played by calcium ions in the signal transduction pathways orchestrating embryonic development. My laboratory studies calcium signaling mainly through the use of aequorins, a group of bioluminescent proteins. They can either be microinjected or transfected into cells, without disturbing function or development. The pattern of luminescence that is emitted by an aequorin-loaded cell, tissue or embryo, reveals changing patterns and levels of free calcium. We are currently studying the role of calcium signaling during basic developmental pattern-forming processes. These include fertilization, segregation, cytokinesis, gastrulation, segmentation, organogenesis and neural induction using mainly zebrafish and Xenopus as vertebrate models. My work also focuses on identifying and understanding the calcium-sensitive target elements interacting with both intracellular and intercellular calcium signals. These include reorganization of cytoskeletal components, membrane remodeling, secretion of morphogens, and localized developmental gene expression via calcium-sensitive transcription factors. Related to these studies, we are also investigating the role played by calcium signaling in two diseases - Polycystic Kidney Disease and Duchene’s Muscular Dystrophy - where links are being established between abnormal calcium regulation and the diseased condition.

8/27/2014 1

Another area of interest is to elucidate the structures and mechanisms of activation of receptor-activated calcium channels in the plasma membrane of non-excitable animal cells. This is being done by targeting aequorins to specific compartments and locations within cells. An additional area of research is the development of the microscopy and image processing techniques required to collect and visualize ultra-low levels of aequorin- generated light emission from living cells and embryos. We have also generated stable lines of transgenic zebrafish where aequorin has been targeted to specific tissue types in order to generate tailor-made fish to further study the role of calcium signaling in development and disease. We have already developed a skeletal muscle- specific line of zebrafish where apoaequorin was successfully expressed in both fast and slow skeletal muscle using a skeletal muscle specific α-actin promoter. We are also in the process of generating transgenic lines of aequorin-expressing Xenopus laevis using both ubiquitous and neuron-specific promoters.

A new focus in my lab is the study of Ca2+ signaling during embryonic stem cell (ESC) differentiation. We are currently working on ways to routinely and reliably image the Ca2+ signaling signatures of ESCs as they differentiate into cardiomyocytes, neurons and glia. In collaboration with the Stem Cell and Regenerative Medicine Consortium (SCRMC) at Hong Kong University, we have begun to develop ESC lines that express apoaequorin. We plan to use these cell lines to characterize the Ca2+ signaling signatures generated by different cell types during the ESC differentiation process.

8/27/2014 2 Awards

1. Visiting Fellowship, New College, Oxford, UK, Fall 2005 - Spring 2006.

2. Croucher Senior Research Fellow 2004 - 2005.

“The Senior Research Fellowship scheme was inaugurated in 1996 to offer full financial support for senior scientists to devote a year to full-time research, away from heavy teaching and administrative responsibilities. These Fellows are selected by the Foundation on the basis of confidential peer assessments and in these exercises, the Foundation is most privileged to be able to enlist help from a number of leading scientists in the international community. As this award appears to be the only of its kind in Hong Kong operated by an independent and well-established scientific grant-making body, and because of its stringent and highly competitive selection process, it is virtually recognised almost as an annual award for distinguished scientists in Hong Kong.”

- The Croucher Foundation.

3. HKUST School of Science Teaching Award (1998).

“Teaching awards in the School of Science at the Hong Kong University of Science and Technology have been presented to three outstanding scholars: Dr Jimmy C. H. Fung (Mathematics), Dr Guochen Jia (Chemistry) and Dr Andrew L. Miller (Biology). Only a few teaching awards are given each year, based on faculty nominations and student evaluations. The current winners were chosen from 120 faculty members in the School of Science.

"The purpose of the awards is to make it clear to our colleagues that teaching is something they must do well," said Prof Michael Loy, Acting Dean of Science. "The awards show that we recognize exceptional teachers."

- Prof. Michael Loy, former Dean of Science, HKUST

4. Duke of Edinburgh Gold Award (1977).

The Duke of Edinburgh Award Scheme provides an enjoyable, challenging and rewarding programme of personal development for young people aged between 14 and 25. It is non-competitive, flexible in design and allows young people to set their own goals. The scheme is delivered through schools, colleges, youth-clubs, voluntary organisations such as scouts and guides and churches. The Gold Award can take up to two years to achieve. Each participant has to cover five different areas of achievement - service, skills, physical recreation, expedition and residential experience.

- The Duke of Edinburgh Award

Professional Activities

1. Founder and Director of the Hong Kong “Joint Universities Summer Teaching Laboratory” (JUSTL) Program at the Marine Biological Laboratory (MBL), Woods Hole, MA, USA. The JUSTL program is an eight- week intensive research and learning experience for PhD students at the MBL, Woods Hole. Six Hong Kong PG students are taken each summer to attend lectures, undergo specialist training and conduct individual research projects under the guidance of the JUSTL Director as well as leading MBL summer scientist mentors drawn from institutions from around the world. The program is funded by the Croucher Foundation and the Government of the Hong Kong SAR and is scheduled and funded to run until the summer of 2015.

2. Hong Kong Director of a “Laboratoire International Associé” (LIA): “Role of Calcium in Cellular Determination and Differentiation”. This international laboratory in funded by the CNRS and HKUST. The French Director is Dr. Marc Moreau of the Centre de Biologie du Développement, Université Paul Sabatier, Toulouse, France.

3. Director of the “Calcium Aequorin Imaging Laboratory”, Division of Life Science, HKUST, Hong Kong.

4. Associate Director of the HKUST Biosciences Central Research Facility (BioCRF) 2009 - 2011 and 2012 - present.

8/27/2014 3 Publications

1. Research Papers 1. Yeuen, M. Y.F., Webb, S.E., Chan, C.M., Thisse, B., Thisse, C., and Miller, A.L. (2013). Characterization of Ca2+ signaling in the external yolk syncytial layer during the late blastula and early gastrula periods of zebrafish development. BBA Molecular Cell Research 1833: 1641-1656. 2. Webb, S.E., Goulet, C., Chan, C.M., Yuen, Y.F., and Miller, A.L. (2013). Biphasic assembly of the contractile apparatus during the first two cell division cycles in zebrafish embryos. Zygote doi: 101017/S0967199413000051. 3. Webb, S.E., Cheung, C.Y., Love, D., and Miller, A.L. (2012). The application of complementary luminescent and fluorescent imaging techniques to visualize nuclear and cytoplasmic Ca2+-signaling during the in vivo differentiation of slow muscle cells in zebrafish embryos under normal and dystrophic conditions. Clin. Exp. Pharm. Physiol. doi10.111/j1440-1681.2011.05582.x. 4. Webb, S.E., Cheung, C.Y., Love, D., and Miller, A.L. (2011). The application of complementary luminescent and fluorescent imaging techniques to visualize nuclear and cytoplasmic Ca2+-signaling during the in vivo differentiation of slow muscle cells in zebrafish embryos under normal and dystrophic conditions. Proceedings of the Australian Physiological Society 42: 29-40. 5. Zhang, J., Webb, S.E., Ma, L., Chan, C., and Miller, A.L. (2011). A necessary role for intracellular Ca2+ transients in initiating the apical-basal thinning of enveloping layer cells during the early blastula period of zebrafish development. Dev. Growth Diff. 53: 679-696. 6. Cheung, C.Y., Webb, S.E., Love, D.R., and Miller A.L. (2011). Visualization, characterization and modulation of Ca2+ signaling during the development of slow muscle cells in intact zebrafish embryos. IJDB 55: 153-174. 7. Mei, W., Marlow, F., Lee, K.W., Marlow, F., Miller, A.L., and Mullins, M.C. (2009). hnRNP I/brom bones is required for calcium-mediated egg activation in the zebrafish. Development 136: 3007-3017. 8. Ma, O., Webb, S.E., Chan, C.M., Zhang, J., and Miller, A.L. (2009). Establishment of a transitory dorsal- biased window of localized Ca2+ signaling in the superficial epithelium following the mid-blastula transition in zebrafish embryos. Dev. Biol. 327: 143-157. 9. Leung, C.F., Miller, A.L., Korzh, V., Chong, S.W., Sleptsova-Friedrich, I. and Webb, S.E. (2009). Visualization of stochastic Ca2+ signals in the formed somites during the early Segmentation Period in intact zebrafish embryos. Dev. Growth & Diff. 51: 617-637. 10. Lam, P.Y., Webb, S.E., Leclerc, C., Moreau, M. and Miller, A.L. (2009). Inhibition of stored Ca2+ release disrupts convergence-related cell movements in the lateral intermediate mesoderm resulting in abnormal positioning and morphology of the pronephric anlagen in intact zebrafish. Dev. Growth & Diff. 51: 429-442. 11. Leclerc, C., Webb, Miller, A.L., and Moreau, M. (2008). An increase in intracellular Ca2+ is involved in pronephric tubule differentiation in the amphibian Xenopus laevis. Dev. Biol. 321: 357-367. 12. Li, W.M., Webb, S.E., Chan, C.M., and Miller, A.L. (2008). Multiple roles of the furrow deepening Ca2+ transient during cytokinesis in zebrafish embryos. Dev. Biol. 316: 228-248 13. Li, W.M., Lee, K.W., Webb, S.E., and Miller, A.L. (2006). The role of localized Ca2+ transients in SNARE- mediated vesicle transport and exocytosis during ingression and zipping of cleavage furrows during cytokinesis in zebrafish embryos. Exp. Cell Res. 312: 3260-3275. 14. Montaville, P. Dai,, Y., Cheung,, C., Giller, K., Becker, S., Michalak, M., Webb, S.E., Miller, A.L. and Krebs, J. (2006). Nuclear translocation of the calcium-binding protein ALG-2 induced by the RNA-binding protein RBM22. Biochim. Biophys. Acta 1763: 1335-1343. 15. Cheung, C.Y., Webb, S.E., Meng, A. and Miller, A.L. (2006). Transient expression of apoaequorin in zebrafish embryos: extending the ability to image calcium transients during later stages of development. Int. J. Dev. Biol. 50: 561-569. 2+ 16. Lee, K.W., Webb, S.E. and Miller, A.L. (2006). Requirement for a localized, IP3R-generated Ca transient during the furrow positioning process in zebrafish zygotes. Zygote 14: 143-155. 17. Lee, K.W., Ho, S.M., Wong, C.H., Webb, S.E. and Miller, A.L. (2004). Characterization of Mid-Spindle Microtubules during Furrow Positioning in Early Cleavage Period Zebrafish Embryos. Zygote 12:221-230. 18. Cheng, J.C., Miller A.L. and Webb, S.E. (2004). The organization and function of microfilaments during epiboly in zebrafish embryos. Dev. Dyn. 231: 313-323.

8/27/2014 4 19. Chan, C., Harland, M.L., Webb, S.E., Miller, A.L. and Barritt, G.J. (2003). Evidence that Ca2+ inflow through liver cell store-operated Ca2+ channels does not require the thapsigargin-sensitive endoplasmic reticulum (Ca2+ + Mg2+)ATP-ase. Cell Calcium 35: 317-331. 2+ 20. Lee, K.W., Webb, S.E. and Miller A.L. (2003). Ca , released via IP3-R, is required for furrow deepening during cytokinesis in zebrafish embryos. Int. J. Dev. Biol. 47: 411-421. 21. Leclerc, C., Lee, M., Webb, S.E., Moreau, M. and Miller, A.L. (2003). Calcium transients triggered by planar signals induce the expression of ZIC3 gene during neural induction in Xenopus. Dev. Biol. 261: 381-390. 22. Webb, S.E. and Miller, A.L. (2003). Imaging intercellular calcium waves during late epiboly in intact zebrafish embryos. Zygote 11(2): 175-182. 23. Leclerc, C., Webb, S.E., Daguzan, C., Moreau, M., and Miller, A.L. (2000). Imaging patterns of calcium transients during neural induction in Xenopus laevis embryos. J. Cell Sci 113: 3519-3529. 24. Leung, C.F., Webb, S.E. and Miller, A.L. (2000). On the mechanism of ooplasmic segregation in single- cell zebrafish embryos. Dev. Growth & Diff. 42: 29-40. 25. Lee, K.W., Webb, S.E. and Miller, A.L. (1999). A wave of free cytosolic calcium traverses zebrafish eggs on activation. Dev. Biol. 214: 168-180. 26. Gilland, E., Miller, A.L., Karplus, E., Baker, R., and Webb, S.E. (1999). Imaging of multicellular large-scale rhythmic calcium waves during zebrafish gastrulation. P.N.A.S. USA. 96: 157-161. 27. Fluck, R.A., Abraham, V., Miller, A.L., and Galione, A. (1999). Microinjection of cyclic ADP-ribose triggers a regenerative wave of Ca2+ release and exocytosis of cortical alveoli in medaka eggs. Zygote 7: 285-292. 28. Leung, C.F., Webb, S.E. and Miller, A.L. (1998). Calcium transients accompany ooplasmic segregation in zebrafish embryos. Dev. Growth & Differ. 40(3): 313-327. 29. Webb, S.E., Lee, K.W., Karplus, E., and Miller, A.L. (1997). Localized calcium transients accompany furrow positioning, propagation, and deepening during the early cleavage period of zebrafish embryos. Dev. Biol. 192: 78-92. 30. Browne, C.L., Créton, R., Karplus, E., Mohler, P.J., Palazzo, R.E., and Miller, A.L. (1996). Analysis of the calcium transient at NEB during the first cell cycle in dividing sea urchin eggs. Biol. Bull. 191: 5-16. 31. Eckberg, W.R., and Miller, A.L. (1995). Propagated and non-propagated calcium transients during egg activation in the annelid Chaetopterus. Dev. Biol. 172: 654-664. 32. Cubitt, A.B., Firtel, R.A., Fischer, G., Jaffe, L.F., Karplus, E., and Miller, A.L. (1995). Patterns of free calcium in developing Dictyostelium. Development 121: 2291-2301. 33. Abraham. V.C., Miller, A.L., and Fluck, R.A. (1995). Microtubule arrays during ooplasmic segregation in the medaka fish egg (Oryzias latipes). Biol. Bull. 188: 136-145. 34. Fluck, R.A., Miller, A.L., Abraham, V.C., and L.F. Jaffe (1994). Calcium buffer injections inhibit ooplasmic segregation in medaka. Biol. Bull. 186: 254-262. 35. Miller, A.L., Fluck, R.A., McLaughlin, J.A., and Jaffe, L.F. (1993). Calcium buffer injections inhibit cytokinesis in Xenopus eggs. J. Cell Science. 106: 523-534. 36. Fluck, R.A., Miller, A.L. and Jaffe, L.F. (1992). High calcium zones at the poles of developing medaka eggs. Biol. Bull. 183: 70-77. 37. Fluck, R.A., Miller, A.L. and Jaffe, L.F. (1991). Slow calcium waves accompany cytokinesis in medaka fish eggs. J. Cell Biol. 115: 1259-1265. 38. Miller, A.L., Smith, G.N., Raven, J.A. and Gow, N.A.R. (1991). Ion currents and the nitrogen status of the roots of Hordeum vulgare and non-nodulated Trifolium repens. Plant, Cell & Environ. 14: 559-567. 39. Speksnijder, J.E., Miller, A.L., Weisenseel, M.H., Chen, T.H., and Jaffe, L.F. (1989). Calcium buffer injections block fucoid egg development by facilitating calcium diffusion. P.N.A.S. USA. 86(17):6607-11. 40. Miller, A.L., (1989). Ion currents and growth regulators in plant root development. Biol. Bull. 176: 65-70. 41. Miller, A.L., and Gow, N.A.R. (1989). Correlation between root-generated ionic currents, pH, Fusicoccin, Indoleactic acid, and growth of the primary root tip of Zea mays. Plant Physiol. 89:1198-1206. 42. Miller, A.L., and Gow, N.A.R. (1989). Correlation between profile of ion-current circulation and root development. Physiol. Planta. 75:102-108.

8/27/2014 5 43. Miller, A.L., Shand, E. and Gow, N.A.R. (1988). Ion currents associated with root tips, emerging laterals and induced wound sites in Nicotiana tabacum: spatial relationship proposed between resulting electrical fields and phytophthoran zoospore infection. Plant, Cell & Environ. 11:21-25. 44. Miller, A.L., Raven, J.A., Sprent, J.I. and Weisenseel, M.H. (1986). Endogenous ion currents traverse growing roots and root hairs of Trifolium repens. Plant, Cell & Environ. 9:79-83.

2. Reviews 1. Webb, S.E., and Miller, A.L. (2014). Retrospective on the development of aequorin and aequorin-based imaging to visualize changes in free Ca2+ in organelles, micro-domains, cells, tissues, organs, and intact organisms. Molecular Reproduction and Development (In Press). 2. Webb, S.E., and Miller, A.L. (2013). Calcium signalling in extra-embryonic domains during early teleost development. Int. Rev. Cell and Molecular Biol. 304: 369-418. 3. Webb, S.E., and Miller, A.L. (2012). Ca2+ signaling during activation and fertilization in the eggs of teleost fish. Cell Calcium 53: 24-31. 4. Webb, S.E., and Miller, A.L. (2012). Aequorin-based genetic approaches to visualize Ca2+ signaling in developing animal systems. Biochim. Biophys. Acta: doi:10.1016/j.bbagen.2011.12.008. 5. Webb, S.E., Fluck, R.A., and Miller, A.L. (2011). Calcium signaling during the early development of medaka and zebrafish. Biochimie. 93: 2112-2125. 6. Miller, A.L. (2010). Calcium signaling during the early meroblastic cleavages of zebrafish and medaka embryos. Front. Biol. 5(4): 283-285. 7. Webb, S.E., Li, W.M. and Miller, A.L. (2008) Calcium signaling during the cleavage period of zebrafish development. Phil. Trans. R. Soc. B. 363: 1363-1369. 8. Moreau, M., Néant, I., Webb, S.E., Miller, A.L. and Leclerc, C. (2008) Calcium signaling during neural induction in Xenopus laevis embryos. Phil. Trans. R. Soc. B. 363: 1371-1375. 9. Webb, S.E. and Miller, A.L. (2007) Ca2+ signaling and early embryonic patterning during zebrafish development. Proceedings of the Australian Physiological Society 38: 43-52. 10. Webb, S.E. and Miller, A.L. (2007) Ca2+ signaling and early embryonic patterning during zebrafish development. Clin. Exp. Pharm. Physiol. 34: 897-904. 11. Webb, S.E. and Miller, A.L. (2006). Ca2+ signaling and early embryonic patterning during the Blastula and Gastrula Periods of Zebrafish and Xenopus development. Biochim. Biophys. Acta 1763: 1192-1208. 12. Leclerc, C., Néant, I., Webb, S.E., Miller, A.L. and Moreau, M. (2006). Calcium transients and calcium signalling during early neurogenesis in the amphibian embryo Xenopus laevis. Biochim. Biophys. Acta 1763: 1184-1191. 13. Webb, S.E. and Miller, A.L. (2006). Ca2+ signaling during vertebrate somitogenesis. Acta Pharmacol. Sin. 27:781-790. 14. Webb, S.E., Moreau, M., Leclerc, C. and Miller, A.L. (2005). Calcium transients and neural induction in vertebrates. Cell Calcium. 37: 375-385. 15. Webb, S.E., and Miller, A.L. (2003). Calcium signaling during embryonic development. Nature Reviews Molecular Cell Biology 4: 539-551. 16. Webb, S.E., Moreau, M., Leclerc, C and Miller, A.L. (2001). Calcium in development: from ion transients to gene expression. Bioessays 23(4): 372-374. 17. Webb, S.E. and Miller, A.L. (2000). Calcium signaling during zebrafish embryonic development. Bioessays 22(2): 113-123.

3. Book Chapters 1. Webb, S.E., Chan, C.M., and Miller, A.L. (2013). Introduction of aequorin into zebrafish embryos for recording Ca2+ signaling events during the first 48 hours of development. Cold Spring Harbor Protocols, CHS Press, Ed. Jan Payrs. 1-4, doi:10.1101/pdb.top066316. 2. Webb, S.E., and Miller, A.L. (2013). Microinjecting holo-aequorin into dechorionated and intact zebrafish embryos. Cold Spring Harbor Protocols, CHS Press, Ed. Jan Payrs. 1-9, doi:10.1101/pdb.top072967.

8/27/2014 6 3. Chan, C.M., Miller, A.L. and Webb, S.E. (2013). Reconstitution of holo-aequorin with apoaequorin mRNA and coelenterazine in zebrafish embryos. Cold Spring Harbor Protocols, CHS Press, Ed. Jan Payrs. 1-5, doi:10.1101/pdb.top072975. 4. Webb, S.E., and Miller, A.L. (2010). Visualization of Ca2+ signaling during embryonic skeletal muscle formation in vertebrates. Eds. Berridge, M.J., Putney, J., Roderick, L., Bootman, M.D. Cold Spring Harbor Perspectives in Biology, CHS Press. doi:101101/cshperspect.a004325. 5. Webb, S.E., Rogers, K.L, Karplus, E., and Miller, A.L. (2010). The use of aequorins to record and visualize Ca2+ dynamics: From subcellular microdomains to whole organisms. In: A Practical Guide to the Study of Calcium in Living Cells. (Ed. M. Whitaker). Methods in Cell Biology Volume 99: Chapter 10: 263-300. 6. Moreau, M., Webb, S.E., Néant, I., Miller, A.L. and Leclerc, C. (2009). Calcium signaling and cell fate determination during neural induction in amphibian embryos. Neural Signaling mechanisms. Chapter 9. Handbook of Neurochem. Mol. Neurobiol., Springer-Verlag, Berlin, pp 4-13. 7. Webb, S.E. and Miller, A.L. (2007). Ca2+ signaling during embryonic cytokinesis in animal systems. In: New Comprehensive Biochemistry Vol. 41: Calcium. A Matter of Life or Death. (Eds, J. Krebs, & M. Michalak). Elsevier. 8. Dodd, A., Greenwood, D.R., Miller, A.L., Webb, S.E., Chambers, S.P., Copp, B.R. and Love, D.R. (2006). Zebrafish: At the nexus of functional and chemical genomics. Biotech. Gen. Eng. Rev. Volume 22, pp 77-99. 2+ 9. Miller, A.L., Karplus, E., and Jaffe, L.F. (1994). Use of aequorin for [Ca ]i imaging. Chapter 13. In: Methods in Cell Biology Volume 40: A Practical Guide to the Study of Ca2+ in Living Cells (ed. R. Nuccitelli), pp 305-338. Academic Press.

4. Peer Reviewed Short Reports in the Biological Bulletin 1. Lee, K.W., Baker, R., Galione, A., Gilland, E.H., Hanlon, R., and Miller, A.L. (1996). Ionophore-induced calcium waves activate unfertilized zebrafish (Danio rerio) eggs. Biol. Bull. 191: 265-267. 2. Fishman, H.M., Krause, T.L., Miller, A.L., and Bittner, G.D. (1995). Retardation of the spread of extracellular Ca2+ into transected, unsealed squid giant axons. Biol. Bull. 189(2):208-9. 2+ 3. Miller, B., Chauhan, A., Jaffe, L.F., and Miller, A.L. (1994). Oscillations in free [Ca ]i during early cell division cycles in Xenopus laevis embryos. Biol. Bull. 187(2):239-40. 4. Miller, A.L., Galione, A., Karplus, E., and Jaffe, L.F. (1993). Mechanically-induced calcium release from Xenopus cell cycle extracts. Biol. Bull. 185: 290-292. 5. Eckberg, W.R., Miller, A.L., Short, L.G., and Jaffe, L.F. (1993). Calcium pulses during the activation of a protostome egg. Biol. Bull. 185:289-290. 6. Rossi, D.J., Kinney, G.A., Karplus, E., Miller, A.L., Jaffe, L.F., and Slater, N.T. (1993). Fluorescence imaging of NMDA receptor-activated calcium influx in granule cells in thin cerebellar slices. Biol. Bull. 185: 295-296. 7. Abraham, V.C., Miller, A.L., Jaffe, L.F. and Fluck, R.A. (1993). Cytoplasmic microtubule arrays in Oryzias latipes (Medaka) eggs during ooplasmic segregation. Biol. Bull. 185: 305-306. 8. Browne, C.L., Miller, A.L., Palazzo, R.E. and Jaffe L.F. (1992). On the calcium pulse during nuclear envelope breakdown (NEB) in sea urchin eggs. Biol. Bull. 183: 370-371. 9. Fluck, R.A., Abraham, V.C., Miller, A.L., and Jaffe, L.F. (1992). Calcium buffer injections block ooplasmic segregation in Oryzias latipes (medaka) eggs. Biol. Bull. 183: 371-372. 10. Miller, A.L., Fluck, R.A. and Jaffe, L. F. (1992). Cell cycle contraction waves in Xenopus are suppressed by injecting calcium buffers. Biol. Bull. 183: 368-369. 11. Jaffe, L.F., Miller, A.L., and Fluck, R.A. (1991). A region of steady high calcium at the vegetal pole of medaka eggs. Biol. Bull. 181: 343-344. 12. Wang, R., Wu, L., Miller, A.L., Arnold, J.M., and Jaffe, L.F. (1991) KCl-induced calcium rise in squid eggs: Measurement of Fura-2 fluorescence. Biol. Bull. 181: 348-349. 13. Woodruff, R. I., Miller, A. L., and Jaffe L. F. (1991). Differences in free calcium concentration between oocytes and nurse cells revealed by corrected aequorin luminescence. Biol. Bull. 181: 349-350. 14. McLaughlin, J.A., Miller, A.L., Fluck, R.A., and Jaffe, L.F. (1991). Calcium buffer injections block cytokinesis in Xenopus eggs. Biol. Bull. 181: 345. 15. Fluck, R.A., Miller, A.L., and Jaffe L.F. (1991). Calcium waves accompany contraction waves in the Oryzias latipes (medaka) blastoderm. Biol. Bull. 181: 352. 8/27/2014 7 16. Miller, A.L., Smith, P.J.S., Rainville, C.A., Shimomura, O. Strumwasser, F. and Jaffe L.F. (1991). Imaging free calcium in cultured Aplysia bag bell neurons. Biol. Bull. 181: 325.

5. Manuscripts under review: Currently none

6. Invited book chapters in preparation 1. Webb, S.E., and Miller, A.L. (2013). Ca2+ signalling during skeletal muscle development. Ed. Philip Poronnik Review.

7. Working Papers: 1. Barnes, M., van RFensburg, G., Li, W-M, Mehmood, K., Mackedenski, S., King, D.T., Miller, A.L, and Lee, C.H. (2014). Insights into the CRD-BP-RNA interaction through site-directed mutagenesis at the GXXG motif in KH domains. (Working paper).

2. Cheng, J.C., Miller A.L. and Webb, S.E. (2013). Characterization and function of surface folds in the plasma membrane of the yolk syncitial layer during epiboly in zebrafish embryos. (Working paper).

3. Coward, K., Young, C. Ponting, C.P., Webb, S.E., Davis, L., Huang, C-J., Chou, C-M., Chan, C.M., Miller, A.L. and Parrington, J. (2013). Identification of the egg activation factor, phospholipase C zeta (PLCζ) in teleost (bony) fish: a testis-specific isoform in the medaka Oryzias latipes but ovary- specific isoforms in the pufferfish Takifugu rubripes and Tetraodon nigroviridis. (Working paper).

Conferences/Retreats Organized at HKUST

(i) 6th Asian Oceania Zebrafish Meeting (AOZM) held at HKUST 19th – 22nd January 2014.

(ii) HKUST/University of Chicago UG Student Exchange Program: Held from 12th June till 26th August 2012. This program cumulated with the “Metcalf Research in China Mini-Symposium” held at HKUST on 22nd August 2012.

(iii) Croucher Foundation Advanced Study Institute: Satellite Workshop - New Developments in Optical Microscopy: Advanced Super-resolution Microscopy. Held from 11th – 12th January, 2012.

(iv) Days of Molecular Medicine 2011: Re-engineering Regenerative Medicine. Held at HKU from 10th – 12th November, 2011. Member of Local Organizing Committee

(v) Croucher Foundation Advanced Study Institute - New Developments in Optical Microscopy: Seeing into the Future of Cell Biology. Held from 11th – 15th January, 2010.

(vi) HKU / HKUST / Cambridge University Joint Research Retreat for Young Scientists 2005. Held from 10th – 12th March, 2005

(vii) Calcium Function in Development, Health and Disease: Applications in Biotechnology 2004. Held from 29th November – 2nd December, 2004.

(viii) Hong Kong/ France Conference 2000. Calcium in Development: From Ion Transients to Gene Expression 2000. Held from 9th – 11th October, 2000.

8/27/2014 8 Invited Talks:

2013 3rd Chinese Zebrafish Conference, Suzhou, China. 11th – 14th October 2013. Miller, A.L. Characterization of Ca2+ signaling in the external yolk syncytial layer (E-YSL) during the late blastula/early gastrula period of zebrafish development. 2012 CNB-CSIC. 18th September, Madrid, Spain. Miller A.L. Calcium release via two pore channels: Implications for developmental Ca2+ signalling.

12th Symposium of the European Calcium Society: Calcium-binding proteins in normal and transformed cells. 9th – 12th September 2012, Toulouse, France. Miller A.L. Session Organizer and Chair: Session VII Calcium Imaging: A brief introduction to calcium imaging.

Cold Spring Harbor Asia: Fishing for Answers: Zebrafish Models of Human Development and Disease. 16th-20th April 2012, Suzhou, China. Miller A.L. Morpholino-based knockdown of two pore channel type 2 in intact zebrafish embryos attenuates the Ca2+ signalling signature generated by slow muscle cells during differentiation and disrupts sarcomeric organization.

2011 4th Annual World Congress of Regenerative Medicine and Stem Cell Biology. 11th-13th November 2011. Beijing, China. Miller A.L. The application of STED nanoscopy to resolve microfilament and micromolecular structures during embryonic stem cell-derived cardiomyocyte differentiation.

The 5th Asia Oceania Zebrafish Meeting. 26th – 29th August 2011. Beijing, China. Miller, A.L. Ca2+ signalling during skeletal muscle differentiation and development in zebrafish.

UCL Cell and Developmental Biology Seminar Series. 17th May 2011. UCL, London. Miller, A.L. Complementary luminescent and fluorescent imaging techniques to visualize nuclear and cytoplasmic Ca2+ signalling during in vivo differentiation of zebrafish slow muscle cells under normal and dystrophic conditions.

The 4th Strategic Coinference of Zebrafish Investigators. 29th January – 2nd February 2011. Asilomar Conference Center, Pacific Grove, CA, USA. Miller, A.L. The application of complementary luminescent and fluorescent imaging techniques to visualize nuclear and cytoplasmic Ca2+ signalling during in vivo differentiation of slow muscle cells in zebrafish embryos.

2010 Australian Physiological Society/Australian Society for Biophysics 2010. 28th November – 1st December 2010, Adelaide, Australia. Miller, A.L. The application of complementary luminescent and fluorescent imaging techniques to visualize nuclear and cytoplasmic Ca2+ signalling during in vivo differentiation of slow muscle cells in zebrafish embryos.

NanoMedicine 2010. 23rd – 25th October 2010, Beijing, China. Miller, A.L.

8/27/2014 9 The use of transfected photoproteins to image calcium dynamics in pathologies related to the mis-regulation of intracellular calcium.

New developments in Optical Microscopy: Seeing into the Future of Cell Biology. 11th - 15th January, 2010, HKUST, Hong Kong. Miller, A.L. Plenary Lecture: Aequorin-based Ca2+ imaging during early zebrafish development.

2009 13th Annual Workshop on Ca2+ Signalling: Calcium and Perception, 19th -22nd October, 2009, Seix, France. Miller, A.L. Plenary Lecture: Spontaneous calcium transients and slow muscle development in zebrafish. 1st Pasteur-Asian Cell Biology Course, 30th March-10th April, 2009, Hong Kong, PRC. Miller, A.L. Ca2+ signaling during embryonic pattern formation.

2008 15th International Symposium on Bioluminescence and Chemoluminescence, 13th-17th May, 2008, Shanghai, China. Miller, A.L., and Webb, S.E. The use of aequorins to image Ca2+ signaling during zebrafish development: Throwing light on embryonic pattern formation.

2007 14th Congress of Calcium Binding Proteins and Calcium Function in Health and Disease, 16th-21st October, 2007, La Palma, Spain. Miller, A.L. Cytokinetic Ca2+ transients: multiple response elements combine to facilitate the separation of daughter cells. The Royal Society Discussion Meeting: Calcium Signals and Developmental Patterning, 19th-20th February, 2007, London, UK. Miller, A.L. Calcium signaling during the cleavage period of zebrafish development

2006 ComBio 2006, 24th – 28th September, 2006, Brisbane, Australia. Webb, S.E. and Miller, A.L. Ca2+ signaling and early embryonic patterning during zebrafish development. 9th European Symposium on Calcium-binding Proteins in Normal and Transformed Cells, 9th – 22nd July, 2006, Strasbourg, France. Webb, S.E. and Miller, A.L. Ca2+ signaling during early embryonic patterning in zebrafish. 20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress, 18th – 23rd June, 2006, Kyoto, Japan. Miller, A.L., Leung, C.F., Cheung, C.Y. and Webb, S.E. Investigation of Ca2+ signals during development. Croucher Advanced Study Institute: Signaling in Cell Growth and Differentiation, 16th – 20th January, 2006, HKUST, Hong Kong Ma, O., Webb, S.E. and Miller, A.L. 2+ IP3R-generated Ca signaling during early zebrafish development.

2005 International Workshop on Molecular Physiology of Intracellular Calcium Signaling. Orange County, Coorg, India. 30th Nov. – 4th December 2005. Andrew L. Miller Calcium signaling during the early development of the zebrafish, Danio rerio.

8/27/2014 10 Gordon Research Conference: Calcium signaling. Queens College Oxford, UK. 24th – 29th July 2005. Andrew L. Miller. Spatially organized calcium signals in early development. 4th European Zebrafish Conference, Dresden, Germany. 13th – 16th July 2005. Andrew L. Miller The role of localized Ca2+ transients in SNARE-mediated vesicle exocytosis during ingression and apposition of cleavage furrows during cytokinesis in zebrafish. Gwangju Institute of Science and Technology, Gwangju, South Korea. 24th – 26th May 2005. Andrew L. Miller The use of the bioluminescent protein aequorin to image Ca2+ signaling during zebrafish development. 14th International Symposium on Calcium and Calcium Binding Proteins in Health & Disease. Banff, Alberta, Canada. 5th – 10th April, 2005 Sarah E. Webb, Marc Moreau, Catherine Leclerc & Andrew L. Miller. The use of zebrafish to investigate the role of Ca2+ signaling during embryonic kidney development. Hong Kong Society for Developmental Biology Inaugural Meeting, HKU, 14th March, 2005 Andrew L. Miller Calcium signaling in early development: Throwing light on key pattern forming events. 10th Medical Research Conference, Department of Medicine, HKU, Queen Mary Hospital, HK. 19th – 20th February, 2005. Andrew L. Miller: The use of zebrafish as a non-mammalian animal model to study calcium-related diseases of humans

2004 Hong Kong Institution of Science 12th Annual Conference 2004, Baptist University of Hong Kong, 30th October, 2004 Andrew L. Miller The Use of Zebrafish as a Model to Study Calcium-related Diseases of Humans. Calcium Regulated Photoproteins and Green-Fluorescent Proteins, Friday Harbor Laboratories, 29th August – 3rd September, 2004 Sarah E. Webb, Karen W. Lee, Christina F. Leung, Jackie C. Cheng, Omi Ma, Chris Cheung & Andrew L. Miller The Use of aequorins to image Ca2+ signaling during zebrafish development MBL General Scientific Meetings, Woods Hole, MA, USA, 9th -11th August, 2004 Andrew L. Miller, Catherine Leclerc, Marc Moreau, & Sarah E. Webb Role of Ca2+ signaling during early pronephric development in zebrafish and Xenopus embryos. 6th Australia & New Zealand Zebrafish Workshop, Whalers Inn Resort, Victor Harbor, Adelaide, Australia, 8th - 10th February, 2004 (1) Chris Y.K. Cheung, Su Ying, Sarah E. Webb, Anming Meng, and Andrew L. Miller Generation of Transgenic Zebrafish That Express Apo-Aequorin in the Muscles.

2003 The Academia Sinica-Hong Kong University of Science and Technology Bilateral Mini-symposium on Zebrafish Development, Academai Sinica, Taipei, Taiwan. 25th August, 2003 Andrew L. Miller Luminescent aequorins: Shedding new light on embryonic calcium signaling. The 5th Australian & New Zealand Zebrafish Workshop. Auckland, New Zealand. 9-11 February, 2003 Jackie C. Cheng, Christina F. Leung, Sarah E. Webb and Andrew L. Miller The Use of Aequorins to Visualize Calcium Signalling During the Gastrula and Segmentation Periods of Zebrafish Development.

2002 Dynamics of Intracellular Signalling. UCL, London, UK. 17th- 20th December, 2002 Christina F.P. Leung, Sarah. E. Webb and Andrew L. Miller An Exploration of the Calcium Signaling during Somitogenesis in Zebrafish.

2001 Physiology in the 21st Century. Chinese University of Hong Kong, New Territories, Hong Kong.12th October 2001

8/27/2014 11 Andrew L. Miller The Use of Aequorins to Image Calcium Signalling during Zebrafish Embryogenesis. CNRS, Université Paul Sabatier, Toulouse, France. Summer 2001 Andrew L. Miller Calcium Signalling during Embryonic Development in Zebrafish. Aquarama 2001. 2nd World Conference on Ornamental Fish Aquaculture Modern Technology for the Future. Singapore Expo, Singapore. 31st May – 2nd June 2001 Leung C.F., Sleptsova-Friedrich I., Webb S.E., Korzh V., and Miller A.L. Calcium Signalling during Somitogenesis in Zebrafish Embryos.

2000 The Hong Kong / France Conference 2000. Ca2+ in Development: from Ion Transients to Gene Expression The Hong Kong University of Science & Technology, Hong Kong. October 9 - 11th, 2000 Sarah E. Webb, Christina F. Leung, and Andrew L. Miller Calcium Signalling During Gastrulation and Segmentation in Zebrafish Embryos. The 12th School of Science Colloquium, HKUST. January 24th, 2000 Andrew L. Miller Calcium Signalling during Zebrafish Embryonic Development.

1999 The Eleventh International Symposium on Calcium-binding Proteins and Calcium Function in Health and Disease. Kisarazu, Japan. October 19th –23rd, 1999 Sarah E. Webb, Christina F. Leung, and Andrew L. Miller Calcium Signalling During the Segmentation Period of Zebrafish Development. The 52nd Annual Meeting of the Japanese Society for Cell Biology. Symposium Number 12: Cytokinesis Hongo Campus, University of Tokyo, Japan. August 27-29th, 1999 Sarah E. Webb, Karen W. Lee, and Andrew L. Miller Calcium Signalling during Cytokinesis in Cleavage Stage Zebrafish Embryos. The Symposium on Development and Genetics of Zebrafish. Institute of Zoology, Academia Sinica, Taipei June 7th –9th, 1999 Andrew L. Miller Calcium Pulses and Waves during Zebrafish Embryonic Development. Aquarama ’99. The 6th International Aquarium Fish and Accessories Exhibition and Conference, World Trade Centre, Singapore. June 3-6th, 1999 Sarah E. Webb, Christina F. Leung, Karen W. Lee and Andrew L. Miller Calcium Signalling During the Segmentation Period of Zebrafish Development.

1998 Department of Biochemistry, The University of Hong Kong, Hong Kong (12th March, 1998). Invited Seminar: The use of aequorins to image calcium waves, pulses, and gradients in living systems. Department of Medical Biochemistry, School of Medicine, Flinders University, Adelaide, Australia (7th July 1998). Invited seminar: Calcium signalling during the gastrula period of zebrafish development. Institute for Molecular Agrobiology, National University of Singapore, Singapore (15th July 1998). Invited seminar: Calcium signalling during the early development of zebrafish.

1997 The 13th International Congress on Developmental Biology and 56th Society of Developmental Biology Annual Meeting, Snowbird, Utah (July 5th to 10th, 1997). Invited Presentation: “Imaging Workshop - Photon imaging of living cells for studying calcium dynamics and gene specific activity”.

1996 Neuroscience Research Center, Beijing Medical University (April 4th-8th, 1996). Invited Seminar: The use of aequorins to image calcium signals in living systems.

8/27/2014 12 1995 The 3rd Stanford International Neuroscience Symposium: “Signal Transduction and Gene Expression in Neurological Disorders”, Shanghai (October 12th-15th, 1995). Platform Presentation: The use of aequorins, both microinjected and transgenically expressed, to image the role of calcium in the injury response and degeneration of neurons.

1994 Boston University School of Medicine: Department of Physiology. Invited Seminar: 13th December 1994: The Use of Aequorins to Image Calcium Gradients, Waves, and Oscillations in Living Systems”. MBL General Scientific Meeting, MBL (August 15th, 1994). 2+ Platform Presentation: Oscillations in Free [Ca ]i during early cell division cycles in Xenopus laevis embryos. 38th Annual Meeting of the Biophysical Society, New Orleans. March 6-10th, 1994. Andrew L. Miller Slow Calcium Waves and Cytokinesis.

1993 The American Society for Cell Biology (ASCB) 33rd Annual Meeting, New Orleans. December 11-15th, 1993. Andrew L. Miller Imaging Free Calcium in Living Cells: Combining Complementary Luminescent and Fluorescent Microscope Techniques. Tulane University, Department of Cell and Molecular Biology, New Orleans (16th December, 1993). Invited Seminar: Calcium Gradients Waves and Oscillations: Ionic Communication within Cells. Boston University Marine Biology Program Seminar Series, MBL (October 1993). Invited Seminar: From Brown Algae Zygotes to Aplysia Bag Cells: The Use of Aequorin for Intracellular Calcium Imaging. MBL General Scientific Meeting, MBL, Woods Hole, MA, USA. August 17th, 1993. Platform Presentation; "Mechanically Induced Calcium Release from Xenopus laevis cell cycle extracts".

1992 MBL General Scientific Meeting, MBL, Woods Hole, MA, USA. August 18th, 1992. Platform Presentation: Cell Cycle Contraction Waves in Xenopus are Suppressed by Injecting Calcium Buffers.

1991 Cornell University, United States Plant, Soil and Nutrition Laboratory, Ithaca, NY (26th April 1991). Invited Seminar: The Role of Ionic Currents in Plant Root Development and Nutrition.

8/27/2014 13 Summary of Research Funding:

Project Title Funding Source Amount (in Duration HK$ unless otherwise stated)

Nikon C1 confocal scanner and controller, and Nikon C1 HKUST REC 2013 HK$289,901 1 Year confocal laser units.

Calcium signaling in glioblastoma multiforme: Combining RGC/ANR (2013) HK$5,805,648 3 Years oncology and neurogenesis with synthetic biology and optical imaging to relate calcium signaling with neural stemness. (HK-based PI – French PI Marc Moreau) Elucidating the mechanism and function of Ca2+ signaling RGC-GRF HK$1,558,858 3 Years during early neurogenesis: Identification of conserved 2012/2013 elements among vertebrates. (PI) Funding to support the 6th Asia Oceania Zebrafish Croucher HK$100,000 1 Year Meeting, HKUST 19th – 22nd January 2014. Foundation, Hong Kong Additional funding to support the 6th Asia Oceania K.C.Wong HK$100,000 1 Year Zebrafish Meeting, HKUST 19th – 22nd January 2014. Foundation

Analysis of the functional interaction between mHtt and CSIC/RGC HK$36,000 2 years DREAM in regulating Ca2+ homeostasis within subcellular 2012/2014 microdomains. (HK-based PI)

One year extension of The Hong Kong Joint Universities Croucher HK$570,000 1 Year Summer Teaching Lab (JUSTL) at the Marine Biological Foundation, Hong Lab, Woods Hole, MA, USA. (PI) Kong CF06/07.SC04

One year extension of The Hong Kong Joint Universities The Government HK$570,000 1 Year Summer Teaching Lab (JUSTL) at the Marine Biological of the HKSAR Lab, Woods Hole, MA, USA. (PI) GMGS06/07.SC01

Cell-based heart regeneration (Co-PI) RGC TBRS 2011 HK$51,096,000 5 years

A continued exploration of the role of calcium signaling RGC-GRF HK$1,380,000 3 years during normal muscle development and in the absence of 2011/2012 dystrophin using intact zebrafish as an animal model. (PI)

Purchase of a Nikon AZ-100 Macro-confocal microscope. HKUST REC 2010 HK$447,000 1 year (PI)

The dual long-term imaging of calcium and gene The Royal Society, GBP12,000 2 years expression in zebrafish embryos. (Co-PI) UK: IJP 2010/R1

A Highly-Sensitive High-Speed Optical and Electro- HKUST RPC 2010 298,000 2 years chemical Platform for Dopamine-mediated Synaptic Plasticity Study. (Co-I)

An Investigation of the Role of Calcium Signaling in RGC GRF 2009 1,491,759 3 years Different Embryonic Domains During Epiboly in Zebrafish Embryos. (PI)

Croucher ASI: New Developments in Optical Microscopy: Croucher 496,000 1 Year Seeing into the Future of Cell Biology. (PI) Foundation, Hong Kong

Establishment of a Multi-Application Microscopy Suite UGC SEG 2008 18,546,894 10 years (MAMS) at HKUST. (PI)

Purchase of a Confocal Live Cell Imaging System. (PI) HKUST 5 “Os” 5,200,000 10 years Equipment Priority

8/27/2014 14 Purchase of a TIRF Microscope Platform. (PI) HKUST REC 2007 840,000 NA

Purchase of a Back Illuminated EMCCD. (PI) HKUST REC 2007 190,000 NA Investigating the role of calcium signaling during the RGC-CERG 1,322,154 3 years blastula period of zebrafish development. (PI) HKUST661707 (2007) A further exploration of the roles of Ca2+ signaling during RGC-CERG 1,422,260 3 years cytokinesis in zebrafish embryos: Extending to a HKUST6416/06M comparison with other fish species. (PI) (2006) Exploring the role of calcium signaling in normal muscle RGC-CERG 939,968 2 years development and in Duchenne Muscular Dystrophy using HKUST6241/04M intact zebrafish as an animal model. (PI) (2004) An investigation of calcium signalling in autosomal RGC-CERG 1,221,000 2 years dominant polycystic kidney disease using zebrafish and HKUST6279/03M Xenopus as animal models. (PI) (2003) An exploration of the furrow positioning signal during RGC-CERG 1,182,609 2 years cytokinesis in zebrafish embryos. (PI) HKUST6214/02M (2002) An investigation of calcium signaling during the RGC-CERG 1,625,420 2 years segmentation period of zebrafish development. (PI) HKUST6106/01M (2001) Subplasmalemmal calcium and activation of store- RGC-CERG 709,500 2 years operates calcium channels. (PI) HKUST6131/99M (1999) A study of calcium signaling during the gastrula period of RGC-CERG 1,195,000 2 years developing zebrafish (Danio rerio). (PI) HKUST6103/98M (1998) A study of the role of slow waves in pattern formation and RGC-CERG 1,149,000 3 years cytokinesis. (PI) HKUST650/96M (1996) Development of a non-mammalian model for biological NSFC/RGC 587,600 3 years and biomedical calcium imaging. (PI) N_HKUST607/01 (2001) Analysis of cytokinetic protein interactions essential for the RGC-DAG 05/06 30,000 1 year terminal phase of cytokinesis. (PI) SC11 (2006) An investigation of calcium signaling during the RGC-DAG 00/01 40,998 1 year segmentation period of zebrafish development. (PI) SC09 (2001) Generation of a transgenic vertebrate model to study RGC-DAG 99/00 62,000 1 year embryonic signaling. (PI) SC11 (2000) Subplasmalemmal calcium and activation of store- RGC-DAG 98/99 35,000 1 year operated calcium channels. (PI) SC30 (1999) A study of the role of slow waves in pattern formation and RGC-DAG 97/98 64,700 1 year cytokinesis. (PI) SC09 (1998) Development of zebrafish expressing the apoaequorin RGC-DAG 95/96 60,000 1 year gene. (PI) SC05 (1996) A study of the dynamic interaction between calcium, UK/HK JRS99/39 73,987 2 years calmodulin, and the cytoskeleton during mitosis and (1999) cytokinesis in zebrafish. (PI) Calcium-regulated genes in development: an integrated Proposal for the €27,850 4 years mechanism for the transcriptional control of cell creation of an determination and differentiation. (Co-PI) Associated (~HK$290,900) International Laboratory (LIA) 8/27/2014 15 Rôle du calcium dans l’expression de gènes responsables PICS N°1730 100,000 1 year de la détermination et de la différenciation neurale chez (2003) l’amphibien (Co-I) Continued: Use of an integrated biological system to investigate the JRS F-HK23/06T 28,000 1 year calcium-regulated genes that are involved in (2006) determination and differentiation during development. (PI) A study of calcium signaling in autosomal dominant JRS F-HK22/01T II 30,600 1 year polycystic kidney disease, year 2. (Co-I) (2003) A study of calcium signaling in autosomal dominant JRS F-HK22/01T 30,600 1 year polycystic kidney disease. (Co-I) (2002) Role of calcium in gene expression during neural JRS F- 81,600 3 years induction in amphibians. (PI) HK98/99.SC06 (1999) Investigation of a possible relationship between patterns Molecular 50,000 1 year of Ca2+ signaling and of early neural gene expression Medicine during rudimentary brain sculpturing in developing Collaborative zebrafish and mouse embryos. (PI) Projects 2005 2006 Subplasmalemmal calcium and activation of store- Australian 794,500 3 years operated calcium channels. (Co-I) Research Council (1999) Calcium function in development, health and disease. (PI) The Hong Kong 100,000 conference Croucher Foundation CF02/03.SC03 (2002) Calcium function in development, health and disease. (PI) K.C. Wong 27,700 conference Foundation (2002) The Hong Kong Joint Universities Summer Teaching Lab The Hong Kong 1,106,118 3 years (JUSTL) at the Marine Biological Lab, Woods Hole, MA, Croucher USA. (PI) Foundation CF06/07.SC04 The Hong Kong Joint Universities Summer Teaching Lab The Government 1,106,118 3 years (JUSTL) at the Marine Biological Lab, Woods Hole, MA, of the HKSAR USA. (PI) GMGS06/07.SC01 Zebrafish Expressing Aequorin: An R24 Pre-Resource NIH R24 US$222,631 1 year (PI) RR10291-01A1 (1995) An Improved Aequorin Method: Research Experience for NSF BIR-9211855 US$9,682 1 year Undergraduates Supplement (PI) (1994) An Improved Aequorin Method: Equipment Supplement. NSF BIR-9211855 US$23,750 22 months (PI) (1993) An Improved Aequorin Method (Co-I) NSF BIR 9211855 US$258,432 3 years (1992)

Grants Under Review: Project Title Funding Source Amount Duration

Nil

8/27/2014 16

Collaborations - Current: Profs. Bernard & Christine Thisse Project: Study of RGS3 and Ca2+ signaling during early Department of Cell Biology zebrafish development. School of Medicine, University of Virginia, Charlottesville, VA 22908, USA Prof. Antony Galione, Project: A study of the role of slow waves in pattern formation Department of Pharmacology, and cytokinesis. Oxford University, UK Co-investigator on RGC CERG HKUST 650/96M Prof. Robert Baker, Project: Exploring calcium signalling during the gastrula phase of Marine Biological Laboratory, zebrafish development. Woods Hole, MA 02543, USA Co-investigator on NIH R24 RR10291-01A1 Drs. Marc Moreau & Catherine Leclerc, Project: Role of calcium in gene expression during neural Centre de Biologie du Dévéloppment, induction in amphibians. Université Paul Sabatier, Toulouse, France Co-investigators on F-HK00/01.SC01 Prof. Greg J. Barritt, Project: A study of the role of subplasmalemmal calcium on Department of Medical Biochemistry, Flinders the activation of store-operated calcium channels. Medical Centre, Flinders University, Adelaide, Co-investigator on RGC CERG HKUST 6131/99M Australia Dr. Donald R. Love Project: Exploring the role of calcium signalling in normal muscle School of Biological Sciences, development and in Duchenne Muscular Dystrophy using intact University of Auckland, New Zealand zebrafish as an animal model. Co-Investigator on RGC CERG HKUST 6241/04M Dr. John Parrington Project: Does phospholipase C zeta play a universal role during Department of Pharmacology, egg activation in vertebrates? University of Oxford, UK Prof. David Whitmore Project: The dual long-term imaging of calcium and gene Department of Anatomy and Developmental expression in zebrafish embryos. Biology, UCL, Co-Investigator on IJP 2010/R1 London, UK. Dr. Mary Mullins University of Pennsylvania School of Medicine, Project: Exploring the role of hnRNP I/brom bones in calcium- Department of Cell and Developmental Biology, mediated egg activation in the zebrafish. Philadelphia, PA, USA

Collaborations - Past: Prof. Michael J. Whitaker, Project: A study of the dynamic interaction between calcium, Department of Physiological Sciences, calmodulin, and the cytoskeleton during mitosis and cytokinesis University of Newcastle, Newcastle, UK in zebrafish. Co-investigator on JRS99/39 Dr. Vladimir Korzh, Project: An investigation of calcium signalling during the Institute of Molecular Agrobiology, National segmentation period of zebrafish development. University of Singapore, Singapore Co-investigator on RGC CERG HKUST 6106/01M Dr. Anming Meng, Project: Development of a non-mammalian model for biological Department of Biological Sciences & and biomedical calcium imaging. Technology, Tsinghua University, Beijing, China Co-investigator on N_HKUST 607/01 Dr. Caroline Brennan, Project: Development of a non-mammalian model for biological School of Biological Sciences, and biomedical calcium imaging and cell signalling during Queen Mary, University of London, UK somite morphogenesis. Prof. Issei Mabuchi, Department of Biology, Project: The role of calcium signalling during cytokinesis. University of Tokyo, Tokyo, Japan

8/27/2014 17 Dr. Bon-Chu Chung, Project: Organisation and function of microfilaments during Institute of Molecular Biology, epiboly. Academia Sinica, Taipei, Taiwan Dr. Jonathan Marchant Department of Pharmacology, Project: Use of aequorins to study calcium signalling during University of Minnesota Medical School, oogenesis in Xenopus. Minneapolis, USA

Teaching

From 1995 till present, at HKUST I have taught various combinations of the following courses:

UG Courses:

1. BIOL 105: Basics in Experimental Biology.

2. BIOL 202: Animal Physiology *.

3. BIOL 222: Animal Physiology Laboratory*.

4. BIOL 309: Developmental Biology.

5. BIOL 316: Advanced Ecology.

6. LIFS 3040: Animal Physiology.

7. LIFS 1010: Appreciation of Biology*.

8. LIFS 1902: General Biology II.

*Course Director

PG Courses:

1. BIOL 507: Workshops in Biology

2. BIOL 516: Advanced Topics in Ecology and Ecological Methodology.

3. BIOL 532: Ecotoxicology*.

4. BIOL 538: Cell Cycle Control.

5. CHEM 543: Advanced Environmental Analytical Chemistry, Microbiology, and Biochemistry.

6. BIOL 604: Special Topics in Developmental Biology.

7. BIOL 613: Scientific Writing*.

8. LIFS 6170: Special Topics.

9. LIFS 6770: Professional Development in Science (Course under construction)*

*Course Director

Was awarded the HKUST School of Science Teaching Award in 1998.

8/27/2014 18 PG Student Training: (1) PG Students Graduated: Name Thesis Title Degree and Date Subsequent position Jeffrey An investigation of the possible function MPhil, 11/13 Visiting Scholar in lab of Prof. Jenkin of Two-Pore Channel 2 (TPC2)- Andrew L. Miller, Division of Life KELU mediated Ca2+ signaling during the Science, HKUST, Hong Kong development of slow muscle cells in zebrafish embryos. Michael Yat Ca2+ signaling during zebrafish epiboly. PhD, 08/12 Postdoc in the Department of Fai YUEN Anatomy, HKU. Chris Yuk Exploring the role of Ca2+ signaling in PhD, 08/09 Postdoc in the Department of Kam slow muscle development using intact Anatomical and Cellular Pathology, CHEUNG zebrafish embryos as an animal model. CUHK, Hong Kong. Jiao ZHANG Ca2+ signaling during EVL formation in MPhil, 08/09 PhD student in the Faculty of zebrafish Medicine at HKU. Penny Pui An investigation of the possible role of MPhil, 08/07 PhD student at the University of Ying LAM Ca2+ signalling during pronephric Wisconsin-Madison, USA. development in zebrafish embryos.

Omicron MA Characterisation of calcium signals during PhD, 01/07 Has a post-doc position in the lab the blastula period of zebrafish (Danio of Prof. Robert Baker, Dept. rerio) embryogenesis. Physiology and Neuroscience, NYU Medical School, New York, USA in June 2007.

Jackie C. Organization and function of PhD, 08/05 Has been awarded Croucher CHENG microfilaments during zebrafish epiboly Fellowship to work as post-doc in the lab of Prof. David Drubin, UC Berkeley, USA.

Karen W. An investigation of the calcium signalling PhD, 08/04 Was awarded a Croucher LEE during cytokinesis in zebrafish Fellowship to work as post-doc in the lab of Dr. Marc Moreau, CNRS Universite Paul Sabaitier, Toulouse, France.

Christina F. An exploration of the calcium signalling PhD, 05/03 Has a post-doc position in the lab LEUNG during somitogenesis in zebrafish (Danio of Prof. Hon Chung Lee, Dept. rerio). Physiology, HKU, Hong Kong.

Chris Yuk Development of alterative methods to MPhil, 08/05 Studied for a PhD in my lab at Kam introduce the Ca2+ sensitive HKUST - see above. CHEUNG bioluminescent complex, aequorin, into zebrafish embryos.

Man Yee The use of ER targeted aequorin to study MPhil, 01/02 Was awarded scholarship to do CHAN store-operated Ca2+ inflow in a liver cell PhD in the lab of Prof. Brian Key, line. University of Queensland, Australia. Michelle The use of open-face explants to study MPhil, 08/01 Was accepted into the lab of Prof. LEE localized Ca2+ signalling during neural H.B. Peng, Dept. Biology, HKUST induction in Xenopus laevis embryos. to do a PhD.

Karen W. An investigation of the roles of calcium MPhil, 07/99 Completed her PhD in Dr. Andrew LEE ions in mechanisms of zebrafish egg L. Miller’s lab in August, 2004 - see activation. above.

Christina F. An exploration of the mechanism of MPhil, 08/98 Completed her PhD in Dr. Andrew LEUNG ooplasmic segregation in zebrafish (Danio L. Miller’s lab in May, 2003 - see rerio). above.

8/27/2014 19

(2) PG Students in Training: Name Project Title Degree Expected Completion Date Jacky Tin Explore role of Ca2+ signaling during bone injury MPhil August 2015 Shing HUNG and regeneration. Harvey Yin Investigation of Ca2+ signaling during hESC MPhil August 2016 Seng CHAN differentiation in cardiomyocytes. Lingyu ZHOU Imaging Ca2+ signaling and the expression of PhD August 2016 clock genes.

(3) Visiting PG Students Supervised: Name Project Title Degree Visiting from Helen Light responsiveness and circadian rhythmicity of PhD Lab of Prof. David MOORE the zebrafish brain. Whitmore, Department of Anatomy and Developmental Biology,UCL, UK. SU Ying Development of a Non-Mammalian Model for PhD Lab. of Prof. Anming Biological and Biomedical Calcium Imaging: Meng, Department of

Biological Sciences & Creating transgenic zebrafish expressing Technology Tsinghua apoaequorin University, Beijing, China.

Student/Staff Training Courses:

06/13- Lingyu ZHOU EMBO Microscopy Workshop, 06/13

06/12 – Jeffrey Jenkin KELU Hong Kong Joint Universities Summer Teaching Laboratory Program, 08/12 MBL, Woods Hole, MA 02543, USA.

06/09 – Michael Yat Fai Hong Kong Joint Universities Summer Teaching Laboratory Program, 08/09 Yuen MBL, Woods Hole, MA 02543, USA.

06/08 – Chris Yuk Kam Hong Kong Joint Universities Summer Teaching Laboratory Program, 08/08 CHEUNG MBL, Woods Hole, MA 02543, USA.

06/04 – Omicron L. Ma Embryology: Concepts & Techniques in Modern Developmental Biology 07/04 course at the MBL, Woods Hole, MA 02543, USA.

05/03 Jackie C. Cheng Analytical and Quantitative Light Microscopy (AQLM) course at the MBL, Woods Hole, MA 02543, USA.

05/02 Jackie C. Cheng Australian Key Centre for Microscopy and Microanalysis Course. Organised by Sydney University Key Centre of Microscopy and Microanalysis and Bio-Rad Pacific Ltd. And held at Chinese University of Hong Kong.

08/01 Jackie C. Cheng Universal Imaging Course: Introduction to Acquisition, Processing and Analysis for Quantitative Digital Microscopy. Held at UI Corp. HQ., Downington, PA, USA.

05/01 Michelle Lee Cold Spring Harbor Course: Cell and Developmental Biology of Xenopus. Held at Cold Spring Harbor, New York, USA. 8/27/2014 20

08/00 Christina F. Leung Neural Development and Genetics of Zebrafish course at the MBL, Woods Hole, MA 02543, USA.

11/99 Karen W. Lee & Australian Key Centre for Microscopy and Microanalysis Course. Dr. Sarah E. Ho Organised by Sydney University Key Centre of Microscopy and Microanalysis and Bio-Rad Pacific Ltd. And held at Hong Kong University.

05/98 Karen W. Lee Analytical and Quantitative Light Microscopy (AQLM) course at the MBL, Woods Hole, MA 02543, USA.

University Service

Professional Level:

Memberships of Learned Societies:

American Association for the Advancement of Sciences. Society for Developmental Biology. Hong Kong Society of Scholars. Society for Experimental Biology. Hong Kong Society of Neuroscience. Society for General Physiology. Marine Biological Laboratory, Woods Hole, MA – The International Society for Bioluminescence. Corporation member since 1990. The European Calcium Society Hong Kong Society for Developmental Biology MBL Neuroimaging Research Cluster Council for Advancement and Support of Education (CASE).

Reviewer for Research Grant Applications:

Hong Kong Research Grants Council Natural Sciences and Engineering Research Council (NERC) of Canada National Science Foundation of the USA The Welcome Trust, UK. Human Frontiers Science Program BBSRC, UK. MRC, UK

Invited Reviewer of Manuscripts for the Following Journals:

Acta Pharmacologica Sinica Developmental Dynamics BioEssays Gene Expression Patterns Biological Bulletin International Journal of Developmental Biology BioTechniques Mechanisms of Development BMC Cell Biology Neuroscience Letters Biochimica et Biophysica Acta Biomedical Optics Cell Calcium Neuro-Signals Current Biology Plant Physiology Development Physiologia Plantarum Developmental Biology Frontiers in Biology Nature Communications Nature Protocols

8/27/2014 21 Departmental Level (Unless otherwise stated, this refers to the Department of Biology):

Departmental Committee Chairs: 2007- 2009 Departmental Recruitment Committee.

2001 Departmental Academic Review and Substantiation Committee.

1999 Postdoctoral Research Fellowship Committee.

Departmental Committee Membership: Present Divisional Postgraduate Committee. Present Divisional Curriculum Development and Teaching Assignment Committee. Past Departmental Space, Equipment and Personnel Committee. Past Departmental Resource Committee. Past Departmental Postgraduate Committee. Past Departmental Academic Review and Substantiation Committee. Past Departmental Recruitment Committee. Past Departmental Undergraduate Committee. Past Departmental ad hoc Curriculum Review Committee. Past Departmental Safety Committee. Past Departmental TLQPR ad hoc Committee. Past Departmental ad hoc Academic Review Committee Past Departmental Teaching and Learning Quality ad hoc Committee. Past Department of Biochemistry Academic Review and Substantiation Committee. Past Department of Biochemistry Merit Salary Review Committee.

Additional Departmental Activities: 2012 – Present Associate Director BioCRF 1995 - 2010 Co-Director of the Departmental Microscopy Facility. Past Departmental Library Co-ordinator. Past Departmental Computer Co-ordinator. Past Co-organizer of a Department of Biology Faculty Retreat held at the Jockey Club Kau Sai Chau Golf Club Function Suit. Past Organized a Departmental Summer Microscopy Workshop in the Central Microscopy Facility in the Department of Biology at HKUST.

8/27/2014 22 School Level: Committee membership: Past School of Science Appointments and Substantiation Committee (SASC) 2007-2009. Past School Search Committee for Headship of the Department of Biology. Past School of Science ad hoc Appeals Committee. Past School of Science Library Coordinator. Past School of Science Biology/Biochemistry Curriculum Committee.

Additional School of Science Activities: 2000 Delivered the 12th School of Science Colloquium: “Calcium Signaling during Zebrafish Embryonic Development 1999 Helped host the School of Science Summer Camp. 1998 Original member of the “Area of Excellence” application to fund “The Molecular Neuroscience Center: Basic Research and TCM-Based Drug Discovery”.

University Level at HKUST: Committee membership: Present University Outreach Team: Visiti to Hanoi and Ho Chi Minh City, 25th 27th September 2013. Present University Technology Review Committee Present University Ranking Committee Present Associate Director BioCRF. Present Member of University Senate. Past Chair: University Appointments and Substantiation Committee (UASC) 01/10/08-01/10/10. Past Senate Committee on Postgraduate Studies (CPS), 01/01/07-31/12/09. Past Senate Postgraduate Admissions Sub-Committee (PAS), 01/05/07-30/04/09. Past University Technology Review Committee (TRC). Past Senate Student Affairs Committee Past Hearing Committee on the Staff Grievance Procedures. Past Senate Library Committee. Past HKUST Social Club

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