DOCSLIB.ORG

The Pleurobemini (Bivalvia&Thinsp;:&Thinsp;Unionida

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
The Pleurobemini (Bivalvia&Thinsp;:&Thinsp;Unionida CSIRO PUBLISHING Invertebrate Systematics, 2018, 32, 689–702 https://doi.org/10.1071/IS17059 The Pleurobemini (Bivalvia : Unionida) revisited: molecular species delineation using a mitochondrial DNA gene reveals multiple conspecifics and undescribed species Kentaro Inoue A,I, David M. Hayes B, John L. Harris C, Nathan A. Johnson D, Cheryl L. Morrison E, Michael S. Eackles E, Tim L. King E, Jess W. JonesF,G, Eric M. HallermanF, Alan D. Christian C,H and Charles R. Randklev A ANatural Resources Institute, Texas A&M University, Dallas, TX 75252, USA. BDepartment of Biological Sciences, Eastern Kentucky University, Richmond, KY 40475, USA. CDepartment of Biological Sciences, Arkansas State University, Jonesboro, AR 72467, USA. DUS Geological Survey, Wetland and Aquatic Research Center, Gainesville, FL 32653, USA. EUS Geological Survey, Leetown Science Center, Kearneysville, WV 25430, USA. FDepartment of Fish and Wildlife Conservation, Virginia Tech, Blacksburg, VA 24061, USA. GUS Fish and Wildlife Service, Blacksburg, VA 24061, USA. HSchool for the Environment, University of Massachusetts, Boston, MA 02125, USA. ICorresponding author. Email: [email protected] Abstract. The Pleurobemini (Bivalvia: Unionida) represent approximately one-third of freshwater mussel diversity in North America. Species identification within this group is challenging due to morphological convergence and phenotypic plasticity. Accurate species identification, including characterisation of currently unrecognised taxa, is required to develop effective conservation strategies because many species in the group are imperiled. We examined 575 cox1 sequences from 110 currently recognised species (including 13 Fusconaia and 21 Pleurobema species) to understand phylogenetic relationships among pleurobemine species (mainly Fusconaia and Pleurobema) and to delineate species boundaries. The results of phylogenetic analyses showed no geographic structure within widespread species and illustrated a close relationship between Elliptio lanceolata and Parvaspina collina. Constraint tests supported monophyly of the genera Fusconaia and Pleurobema, including the subgenus P. (Sintoxia). Furthermore, results revealed multiple conspecifics, including P. hanleyianum and P. troschelianum, P. chattanoogaense and P. decisum, P. clava and P. oviforme, P. rubrum and P. sintoxia, F. askewi and F. lananensis, and F. cerina and F. flava. Species delimitation analyses identified three currently unrecognised taxa (two in Fusconaia and one in Pleurobema). Further investigation using additional genetic markers and other lines of evidence (e.g. morphology, life history, ecology) are necessary before any taxonomic changes are formalised. Additional keywords: DNA barcode, freshwater mussels, generalised mixed Yule-coalescent, molecular systematics, phylogenetics, species delimitation. Received 1 July 2017, accepted 25 October 2017, published online 1 June 2018 Introduction (Bickford et al. 2006). In relatively immobile organisms, there External morphologies, such as molluscan shells and arthropod is often high morphological variation due to interaction between exoskeletons, are the primary characteristics used by taxonomists genetic factors and ambient environmental conditions. For to describe and identify species. However, external morphology example, in freshwater mussels (Bivalvia: Unionida; hereafter, can be influenced by the environment, biological interactions, freshwater mussels), it is known that shell shape and sculpture can and genetics (i.e. phenotypic plasticity) (Via et al. 1995). vary with stream position and hydrodynamic conditions Thus, a single nominal species may exhibit multiple distinct (Ortmann 1920; Watters 1994). At the same time, evolutionary morphologies. In contrast, cryptic diversity can arise under convergence in shell morphologies is relatively common among those same conditions leading to cases where two or more freshwater mussel species (see Haag 2012 for a summary). Thus, distinct species share similar morphological characteristics, phenotypic plasticity combined with the existence of cryptic making them externally indistinguishable (i.e. cryptic species) species makes morphology-based identification of certain Journal compilation Ó CSIRO 2018 www.publish.csiro.au/journals/is 690 Invertebrate Systematics K. Inoue et al. taxonomic groups of freshwater mussels a challenge and likely We used molecular phylogenetic and species delimitation leads to under- and overestimates of the total species diversity approaches to revisit phylogenetic differentiation among within given taxa. The advent of species delimitation via species within Pleurobemini using additional species/ molecular genetic techniques can address both issues by populations collected for this study and data from previous determining putative species boundaries within and among studies (Tables S1 and S2). Our objectives were to: (1) delimit species and allow hidden biodiversity to be uncovered. putative species within Pleurobemini, focusing on the genera Freshwater mussels reach their greatest species diversity Fusconaia and Pleurobema, and (2) examine cryptic diversity in North America, with ~300 currently recognised species within Pleurobemini in the context of phylogenetic patterns (Haag and Williams 2014), although diversity may be higher among other mussel species. than is currently recognised due to imperfect understanding of mussel taxonomy, which is based primarily on shell morphology Methods (Williams et al. 1993; Turgeon et al. 1998; Graf and Cummings 2007). For example, recent molecular phylogenetics and Taxon sampling, sample preparation, and sequencing species delimitation studies have uncovered cryptic diversity We included 575 specimens (110 species, 35 genera/subgenera) among freshwater mussels (Serb et al. 2003; Gangloff et al. (Tables S1 and S2), of which 223 specimens (105 species, 35 2006; Jones et al. 2006; Inoue et al. 2013; Pfeiffer et al. 2016; genera/subgenera) (Table S2) were obtained from GenBank, Perkins et al. 2017), providing empirical support that mussel and we assimilated previously unpublished data for 352 diversity within North America continues to be underestimated. specimens from 42 species representing 11 genera for the The current classification within the family Unionidae current study. A list of taxa, waterbody of collection, unique recognises seven subfamilies, of which three occur in North identifiers, and GenBank accession numbers are provided in America: Ambleminae, Anodontinae, and Gonideinae (Lopes- Table S1. We extracted genomic DNA from mantle tissue via Lima et al. 2017). Ambleminae contains four tribes: Amblemini, a CTAB/chloroform extraction method (Saghai-Maroof et al. Lampsilini, Pleurobemini, and Quadrulini (Lopes-Lima et al. 1984) or using the PureGene DNA extraction kit (Gentra 2017). Many species within these groups have not been examined Systems, Minneapolis, MN) following manufacturer’s using molecular phylogenetics, resulting in their exact taxonomic guidelines. After quantification using fluorescence (Labarca placement being uncertain. Resolving these taxonomic and and Paigen 1980), extracted DNA was diluted to 10 ng mL–1 systematic uncertainties is important for the development of and used as a template in polymerase chain reaction (PCR) for the sound conservation actions, such as listing under the US mtDNA cytochrome c oxidase subunit I (cox1). We used cox1 Endangered Species Act and design of associated recovery plans. primers and the thermal conditions for PCR described in The Pleurobemini was recognised as a distinct subfamily Campbell et al.(2005). Products from PCR were visualised by Hannibal (1912). Campbell and Lydeard (2012a) provided using 1% agarose gel electrophoresis and purified with the first modern review of the systematic and taxonomic QIAquick Gel Extraction Kit (Qiagen) or ExoSAP-IT history of this group using external morphology, anatomy, (Affymetrix). We performed cycle sequencing reactions with and molecular phylogenetics, which resulted in several BIGDYE 3.1 and sequenced bidirectionally using an ABI Genetic nomenclatural revisions. For example, the genera Quincuncina Analyzer (Applied Biosystems). Sequences were visually aligned and Lexingtonia were synonomised within Fusconaia, and in DNADYNAMO (Blue Tractor Software, Ltd) and an open- Pleuronaia was elevated from synonomy within Fusconaia. reading frame was verified. We used MAFFT 7 (Katoh and Several genera within Pleurobemini were removed from the Standley 2013) to perform multiple sequence alignment. tribe, which included Cyclonaias Ortmann & Walker, 1922, Uniomerus Conrad, 1853, and the new genus Reginaia Campbell & Lydeard, 2012. Furthermore, due to phylogenetic Phylogeny estimation distinctiveness, Campbell and Lydeard (2012a) suggested The primary aim of this study was to examine phylogenetic elevation of the subgenera Eurynia and Sintoxia from relationships within two genera (Fusconaia and Pleurobema) synonomy within Elliptio and Pleurobema, respectively, and in Pleurobemini; therefore, we included 62 species from potential placement of Pleurobema collina in a new genus. 10 pleurobemine genera/subgenera (Elliptio, Elliptoideus, Subsequently, Perkins et al.(2017) analysed the molecular Eurynia, Fusconaia, Hemistena, Parvaspina, Plethobasus, systematics of P. collina and described the new genus Pleurobema, Pleurobema (Sintoxia), and Pleuronaia) and 47 Parvaspina, which included the species previously recognised species representing three subfamilies in Unionidae
Load more

Recommended publications
  • DISTRIBUTICNAL ANALYSIS of the FP.Esh"WA'l'e.H MUSSEL FAUNA
    DISTRIBUTICNAL ANALYSIS OF THE FP.ESh"WA'l'E.H MUSSEL FAUNA OF THE TENNESSEE RT·lER SiSTE.'1, WIT"'.d SPECIAL REF:::::",.ENCE TO POSSIBLE LIMITI:~G EFFECTS OF SILTATIO.\l by Sally D. Dennis Dissertation su~mitted to the Faculty of the Virginia Polytechnic Institute and Stat~ University in partial fulfillment of the requirements for the degree of DOCIOR OF PHILOSOPHY in zoolog'J APPROVED: E. F. Benfi~la, Chairman r A. L. 3u1keiJ¢, Jr. A. c. Eehdncks l 1 R. A Paterson D. i1. ??irter H. van der Scha..:.:e August, 1984 B2.acksburg, Virginia DISTRIBUTIONAL ANALYSIS OF THE FRESHWATER MUSSEL FAUNA OF THE TENNESSEE RIVER SYSTEM, WITH SPECIAL REFERENCE TO POSSIBLE LIMITING EFFEcrS OF SILTATION by sally D. Dennis (ABSTRAcr} Mussel studies in the Tennessee River drainage (1973 - 1982} examined ecology and distribution and investigated factors limiting to distribution~ This river system presently supports 71 freshwater mussel species, 25% of which are endemic to the Cumberlandian Region. Species have been extirpated from this drainage within the past 60 years, however the number of extant species remains high. The major impact of man's activities has been reduction of available habitat Past and present distribution records indicate that mussel species assemblages are determined by geologic history and stream size. Although some overlap of species exists among stream size categories, there is no longer a continuous gradation from one category to the next; mussels exist in isolated conmunities separated by nonproductive river reaches. Productive reaches supporting more than 60 mussel species once existed in habitats spanning the transition from medium to large rivers; reaches now altered by impoundment.
    [Show full text]
  • Caracara Cheriway
    Rare Animal Fact Sheet IMBIV31060 Louisiana Department of Wildlife and Fisheries Natural Heritage Program Obovaria unicolor Alabama Hickorynut Identification: This mussel has a round or elliptical shape; shell is nearly smooth, shiny, brown to yellowish brown with rays, young shells are green with green rays; mother-of-pear is pink but sometimes white or blue. Measurements: A small mussel, 1.5 to 2 inches in length. Taxonomic comments: No recognized subspecies. Status: Global ranking is G3 and state rank is S1. Habitat: Sand and gravel bottoms in river systems. Range: Eastern gulf drainages throughout Alabama, Louisiana, Mississippi, and Oklahoma. Food habits*: Mussels are continually pumping water through their siphon. Their diet is composed of the various micropscopic plants and animals from the water and organic matter from stream bottoms that they filter from this water. Reproduction: Sexes are separate. No information on host fish available. Reason for decline: 1) The damming of stream and rivers results in the loss of mussel and host fish habitat. 2) Channelization of large stream habitat. 3) Declining water quality as a result of siltation (reduces oxygen level) and heavy recreational use of habitat. Interesting facts: This species is common only in the Sipsey River in Alabama. It is believed to have been uncommon or historically rare throughout the rest of its range. * Indicates generalized information for freshwater mussels in the absence of information specific to this species Rare Animal Fact Sheet IMBIV31060 Known distribution in Louisiana: Dates of documented observations are: 1977, 1979, 1988, and 1994 References McCullagh, W.H., J.D. Williams, S.W.
    [Show full text]
  • Indiana Species April 2007
    Freshwater Mussels of Indiana April 2007 The Wildlife Diversity Section (WDS) is responsible for the conservation and management of over 750 species of nongame and endangered wildlife. The list of Indiana's species was compiled by WDS biologists based on accepted taxonomic standards. The list will be periodically reviewed and updated. References used for scientific names are included at the bottom of this list. ORDER FAMILY GENUS SPECIES COMMON NAME STATUS* Unionoida Unionidae Actinonaias ligamentina mucket Alasmidonta marginata elktoe Alasmidonta viridis slippershell mussel Amblema plicata threeridge Anodonta suborbiculata flat floater Anodontoides ferussacianus cylindrical papershell Arcidens confragosus rock pocketbook Cyclonaias tuberculata purple wartyback Cyprogenia stegaria fanshell SE/FE Ellipsaria lineolata butterfly Elliptio crassidens elephantear Elliptio dilatata spike Epioblasma obliquata perobliqua white catspaw SE/FE Epioblasma torulosa rangiana northern riffleshell SE/FE Epioblasma torulosa torulosa tubercled blossom SE/FE Epioblasma triquetra snuffbox SE Fusconaia ebena ebonyshell Fusconaia flava Wabash pigtoe Fusconaia subrotunda longsolid SE Lampsilis abrupta pink mucket SE/FE Lampsilis cardium plain pocketbook Lampsilis fasciola wavyrayed lampmussel SC Lampsilis ovata pocketbook Lampsilis siliquoidea fatmucket Lampsilis teres yellow sandshell Lasmigona complanata white heelsplitter Lasmigona compressa creek heelsplitter Lasmigona costata flutedshell Leptodea fragilis fragile papershell (Freshwater Mussels of Indiana
    [Show full text]
  • Francis Marion National Forest Freshwater Mussel Surveys: Final Report
    Francis Marion National Forest Freshwater Mussel Surveys: Final Report Contract No. AG-4670-C-10-0077 Prepared For: Francis Marion and Sumter National Forests 4931 Broad River Road Columbia, SC 29212-3530 Prepared by: The Catena Group, Inc. 410-B Millstone Drive Hillsborough, NC 27278 November 2011 TABLE OF CONTENTS 1.0 INTRODUCTION ................................................................................................... 1 1.1 Background and Objectives ................................................................................. 1 2.0 STUDY AREA ........................................................................................................ 2 3.0 METHODS .............................................................................................................. 2 4.0 RESULTS ................................................................................................................ 3 5.0 DISCUSSION ........................................................................................................ 19 5.1 Mussel Habitat Conditions in the FMNF (Objective 1) ..................................... 19 5.2 Mussel Fauna of the FMNF (Objective 2) ......................................................... 19 5.2.1 Mussel Species Found During the Surveys ................................................ 19 5.3 Highest Priority Mussel Fauna Streams of the FMNF (Objective 3) ................. 25 5.4 Identified Areas that Warrant Further Study in the FMNF (Objective 4) .......... 25 6.0 LITERATURE CITED .........................................................................................
    [Show full text]
  • Tennessee's Extinct Species
    Tennessee's Extinct Species The following species Birds: once occurred in Carolina parakeet Conuropsis carolinensis Ectopistes migratorius Tennessee and are now Passenger pigeon believed to be extinct. Mammals: Following this list are two Eastern elk species descriptions-one Fishes: describing the Carolina Harelip sucker parakeet and another describing the extinct Mussels: Acornshell Epioblasma haysiana freshwater mussels Angled riffleshell Epioblasma biemarginata of Tennessee. Cumberland leafshell Epioblasma stewardsoni Leafshell Epioblasma flexuosa Narrowcat's paw Epioblasma lenoir Rough rockshell Quadrula tuberosa Round combshell Epioblasma personata Sugarspoon Epioblasma arcaeformis Tennessee riffleshell Epioblasma propinqua Carolina Parakeet Status Habitat The Carolina parakeet is an The Carolina parakeet was found Learn rrwreabout extinct species. in riverine forests, cypress swamps, Tennessee's diverse and other woodlands over much of Description the Eastern and Midwest Regions of ecosyster.n3.Su~ort The Carolina parakeet was a the United States. It was the only conservation in your small parrot, about 12inches in parrot native to the United States. community and state! length. Its head was lemon yellow, The parakeets rested at night in with an orange forehead and cheeks. groups, with as many as 30 birds The rest of its body was green. Its sleeping inside one hollowtree, while legs and beak were pale pinkish- others would hang on the outside. white. These curious birds lived and Nests were placed in hollowtrees, traveled in flocks. and three to five white eggs were laid. Up to 50 nests were often crowded into one tree. Role in the Ecosystem Carolina parakeets enjoyed a variety of different foods-apples, peaches, mulberries, pecans, grapes, dogwood fruit, and grains.
    [Show full text]
  • Status and Population Genetics of the Alabama Spike (Elliptio Arca) in the Mobile River Basin
    STATUS AND POPULATION GENETICS OF THE ALABAMA SPIKE (ELLIPTIO ARCA) IN THE MOBILE RIVER BASIN A Thesis by DANIEL HUNT MASON Submitted to the Graduate School at Appalachian State University in partial fulfillment of the requirements for the degree of MASTER OF SCIENCE August, 2017 Department of Biology STATUS AND POPULATION GENTICS OF THE ALABAMA SPIKE (ELLIPTIO ARCA) IN THE MOBILE RIVER BASIN A Thesis by DANIEL HUNT MASON August, 2017 APPROVED BY: Michael M. Gangloff, Ph.D. Chairperson, Thesis Committee Matthew C. Estep, Ph.D. Member, Thesis Committee Lynn M. Siefermann, Ph.D. Member, Thesis Committee Zack E. Murrell, Ph.D. Chairperson, Department of Biology Max C. Poole, Ph.D. Dean, Cratis D. Williams School of Graduate Studies Copyright by Daniel Hunt Mason 2017 All Rights Reserved Abstract STATUS AND POPULATION GENETICS OF THE ALABAMA SPIKE (ELLIPTIO ARCA) IN THE MOBILE RIVER BASIN Daniel H. Mason B.A., Appalachian State University M.A., Appalachian State University Chairperson: Dr. Michael M. Gangloff Declines in freshwater mussels (Bivalvia: Unionioda) are widely reported but rarely rigorously tested. Additionally, the population genetics of most species are virtually unknown, despite the importance of these data when assessing the conservation status of and recovery strategies for imperiled mussels. Freshwater mussel endemism is high in the Mobile River Basin (MRB) and many range- restricted taxa have been heavily impacted by riverine alterations, and many species are suspected to be declining in abundance, including the Alabama Spike (Elliptio arca). I compiled historical and current distributional data from all major MRB drainages to quantify the extent of declines in E.
    [Show full text]
  • Final Report- HWY-2009-16 Propagation and Culture of Federally Listed Freshwater Mussel Species
    Final Report- HWY-2009-16 Propagation and Culture of Federally Listed Freshwater Mussel Species Prepared By Jay F- Levine, Co-Principal Investigator1 Christopher B- Eads, Co-Investigator1 Renae Greiner, Graduate Student Assistant1 Arthur E- Bogan, Co- Investigator2 1North Carolina State University College of Veterinary Medicine 4700 Hillsborough Street Raleigh, NC 27606 2 NC State Museum of Natural Sciences 4301 Reedy Creek Rd- Raleigh, NC 27607 November 2011 Technical Report Documentation Page 1- Report No- 2-Government Accession No- 3- Recipient’s Catalog No- FHWA/NC/2009-16 4- Title and Subtitle 5- Report Date Propagation and Culture of Federally Listed Freshwater November 2011 Mussel Species 6-Performing Organization Code 7- Author(s) 8-Performing Organization Report No- Jay F- Levine, Co-Principal Investigator Arthur E- Bogan, Co-Principal Investigator Renae Greiner, Graduate Student Assistant 9- Performing Organization Name and Address 10- Work Unit No- (TRAIS) North Carolina State University College of Veterinary Medicine 11- Contract or Grant No- 4700 Hillsborough Street Raleigh, NC 27606 12- Sponsoring Agency Name and Address 13-Type of Report and Period Covered North Carolina Department of Transportation Final Report P-O- Box 25201 August 16, 2008 – June 30, 2011 Raleigh, NC 27611 14- Sponsoring Agency Code HWY-2009-16 15- Supplementary Notes 16- Abstract Road and related crossing construction can markedly alter stream habitat and adversely affect resident native flora. The National Native Mussel Conservation Committee has recognized artificial propagation and culture as an important potential management tool for sustaining remaining freshwater mussel populations and has called for additional propagation research to help conserve and restore this faunal group.
    [Show full text]
  • IN the KIAMICHI RIVER, OKLAHOMA PROJECTTITLE: Habitat Use and Reproductive Biology of Arkansia Whee/Eri (Mollusca: Unionidae) in the Kiamichi River, Oklahoma
    W 2800.7 E56s No.E-12 1990/93 c.3 OKLAHOMA o HABITAT USE AND REPRODUCTIVE BIOLOGY OF ARKANSIA WHEELERI (MOLLUSCA: UNIONIDAE) IN THE KIAMICHI RIVER, OKLAHOMA PROJECTTITLE: Habitat use and reproductive biology of Arkansia whee/eri (Mollusca: Unionidae) in the Kiamichi River, Oklahoma. whee/eri is associated. In its optimal habitat, A. whee/eri is always rare: mean relative 2 abundance varies from 0.2 to 0.7% and the average density is 0.27 individuals/m • In addition, shell length data for live Amb/ema plicata, a dominant mussel species in the Kiamichi River, indicate reduced recruitment below Sardis Reservoir. Much of the Kiamichi River watershed remains forested and this probably accounts for the high diversity and general health of its mussel community in comparison to other nearby rivers. Program Narrative Objective To determine the distribution, abundance and reproductive biology of the freshwater mussel Arkansia whee/eri within different habitats in the Kiamichi River of Oklahoma. Job Procedures 1. Characterize microhabitats and determine the effects of impoundment. 2. Determine movement, growth, and survivorship of individuals. 3. Identify glochidia and fish host. 4. Examine impact of Sardis Reservoir on the populations. 5. Determine historic and current land use within the current range of Arkansia whee/eri in the Kiamichi River. A. Introduction Arkansia (syn. Arcidens) whee/eri, the Ouachita rock pocketbook, is a freshwater mussel. Originally named Arkansia whee/eri by Ortmann and Walker in 1912, Clarke (1981, 1985) recognized Arkansia as a subgenus of Arcidens. The species is considered by Clarke to be distinct. However, Turgeon et al (1988) have continued to use the binomial Arkansia whee/eri.
    [Show full text]
  • Responses to Elevated CO2 Exposure in a Freshwater Mussel, Fusconaia Flava
    J Comp Physiol B (2017) 187:87–101 DOI 10.1007/s00360-016-1023-z ORIGINAL PAPER Responses to elevated CO2 exposure in a freshwater mussel, Fusconaia flava Jennifer D. Jeffrey1 · Kelly D. Hannan1 · Caleb T. Hasler1 · Cory D. Suski1 Received: 7 April 2016 / Revised: 29 June 2016 / Accepted: 19 July 2016 / Published online: 29 July 2016 © Springer-Verlag Berlin Heidelberg 2016 Abstract Freshwater mussels are some of the most reduce their investment in non-essential processes such as imperiled species in North America and are particularly shell growth. susceptible to environmental change. One environmen- tal disturbance that mussels may encounter that remains Keywords Chitin synthase · Heat shock protein 70 · understudied is an increase in the partial pressure of CO2 Metabolic rate · Bivalve (pCO2). The present study quantified the impacts of acute (6 h) and chronic (up to 32 days) exposures to elevated pCO2 on genes associated with shell formation (chitin syn- Introduction thase; cs) and the stress response (heat shock protein 70; hsp70) in Fusconaia flava. Oxygen consumption (MO2) Freshwater mussels have their highest abundance and was also assessed over the chronic CO2 exposure period. diversity in North America, and provide many important Although mussels exhibited an increase in cs following ecological functions (Williams et al. 1993; Bogan 2008). an acute exposure to elevated pCO2, long-term exposure For example, freshwater mussels filter large volumes of resulted in a decrease in cs mRNA abundance, suggest- water daily, remove bacteria and particles from the water ing that mussels may invest less in shell formation during column, and generate nutrient-rich areas (Vaughn and chronic exposure to elevated pCO2.
    [Show full text]
  • Restoring the Endangered Oyster Mussel (Epioblasma Capsaeformis) to the Upper Clinch River, Virginia: an Evaluation of Population Restoration Techniques Caitlin S
    RESEARCH ARTICLE Restoring the endangered oyster mussel (Epioblasma capsaeformis) to the upper Clinch River, Virginia: an evaluation of population restoration techniques Caitlin S. Carey1,2,3,JessW.Jones4, Robert S. Butler5, Eric M. Hallerman6 From 2005 to 2011, the federally endangered freshwater mussel Epioblasma capsaeformis (oyster mussel) was reintroduced at three sites in the upper Clinch River, Virginia, using four release techniques. These release techniques were (1) translocation of adults (site 1, n = 1418), (2) release of laboratory-propagated sub-adults (site 1, n = 2851), (3) release of 8-week-old laboratory-propagated juveniles (site 2, n = 9501), and (4) release of artificially infested host fishes (site 3, n = 1116 host fishes). These restoration efforts provided a unique research opportunity to compare the effectiveness of techniques used to reestablish populations of extirpated and declining species. We evaluated the relative success of these four population restoration approaches via monitoring at each release site (2011–2012) using systematic 0.25-m2 quadrat sampling to estimate abundance and post-release survival. Abundances of translocated adult and laboratory-propagated sub-adult E. capsaeformis at site 1 ranged 577–645 and 1678–1700 individuals, respectively, signifying successful settlement and high post-release survival. Two untagged individuals (29.1 and 27.3 mm) were observed, indicating that recruitment is occurring at site 1. No E. capsaeformis were found at sites where 8-week-old laboratory-propagated juveniles (site 2) and artificially infested host fishes (site 3) were released. Our results indicate that translocations of adults and releases of laboratory-propagated sub-adults were the most effective population restoration techniques for E.
    [Show full text]
  • Guiding Species Recovery Through Assessment of Spatial And
    Guiding Species Recovery through Assessment of Spatial and Temporal Population Genetic Structure of Two Critically Endangered Freshwater Mussel Species (Bivalvia: Unionidae) Jess Walter Jones ( [email protected] ) United States Fish and Wildlife Service Timothy W. Lane Virginia Department of Game and Inland Fisheries N J Eric M. Hallerman Virginia Tech: Virginia Polytechnic Institute and State University Research Article Keywords: Freshwater mussels, Epioblasma brevidens, E. capsaeformis, endangered species, spatial and temporal genetic variation, effective population size, species recovery planning, conservation genetics Posted Date: March 16th, 2021 DOI: https://doi.org/10.21203/rs.3.rs-282423/v1 License: This work is licensed under a Creative Commons Attribution 4.0 International License. Read Full License Page 1/28 Abstract The Cumberlandian Combshell (Epioblasma brevidens) and Oyster Mussel (E. capsaeformis) are critically endangered freshwater mussel species native to the Tennessee and Cumberland River drainages, major tributaries of the Ohio River in the eastern United States. The Clinch River in northeastern Tennessee (TN) and southwestern Virginia (VA) harbors the only remaining stronghold population for either species, containing tens of thousands of individuals per species; however, a few smaller populations are still extant in other rivers. We collected and analyzed genetic data to assist with population restoration and recovery planning for both species. We used an 888 base-pair sequence of the mitochondrial NADH dehydrogenase 1 (ND1) gene and ten nuclear DNA microsatellite loci to assess patterns of genetic differentiation and diversity in populations at small and large spatial scales, and at a 9-year (2004 to 2013) temporal scale, which showed how quickly these populations can diverge from each other in a short time period.
    [Show full text]
  • REPORT FOR: Preliminary Analysis for Identification, Distribution, And
    REPORT FOR: Preliminary Analysis for Identification, Distribution, and Conservation Status of Species of Fusconaia and Pleurobema in Arkansas Principle Investigators: Alan D. Christian Department of Biological Sciences, Arkansas State University, P.O. Box 599, State University, Arkansas 72467; [email protected]; Phone: (870)972-3082; Fax: (870)972-2638 John L. Harris Department of Biological Sciences, Arkansas State University, P.O. Box 599, State University, Arkansas 72467 Jeanne Serb Department of Ecology, Evolution, and Organismal Biology, Iowa State University, 251 Bessey Hall, Ames, Iowa 50011 Graduate Research Assistant: David M. Hayes, Department of Environmental Science, P.O. Box 847, State University, Arkansas 72467: [email protected] Kentaro Inoue, Department of Environmental Science, P.O. Box 847, State University, Arkansas 72467: [email protected] Submitted to: William R. Posey Malacologist and Commercial Fisheries Biologist, AGFC P.O. Box 6740 Perrytown, Arkansas 71801 April 2008 EXECUTIVE SUMMARY There are currently 13 species of Fusconaia and 32 species of Pleurobema recognized in the United States and Canada. Twelve species of Pleurobema and two species of Fusconaia are listed as Threatened or Endangered. There are 75 recognized species of Unionidae in Arkansas; however this number may be much higher due to the presence of cryptic species, many which may reside within the Fusconaia /Pleurobema complex. Currently, three species of Fusconaia and three species of Pleurobema are recognized from Arkansas. The true conservation status of species within these genera cannot be determined until the taxonomic identity of populations is confirmed. The purpose of this study was to begin preliminary analysis of the species composition of Fusconaia and Pleurobema in Arkansas and to determine the phylogeographic relationships within these genera through mitochondrial DNA sequencing and conchological analysis.
    [Show full text]