Anti-PADI4 / PAD4 Antibody (ARG40521)

Product datasheet [email protected]

ARG40521 Package: 100 μl anti-PADI4 / PAD4 antibody Store at: -20°C

Summary

Product Description Rabbit Polyclonal antibody recognizes PADI4 / PAD4

Tested Reactivity Hu

Tested Application ICC/IF, WB

Host Rabbit

Clonality Polyclonal

Isotype IgG

Target Name PADI4 / PAD4

Antigen Species Human

Immunogen Recombinant fusion protein corresponding to aa. 1-250 of Human PADI4 (NP_036519.2).

Conjugation Un-conjugated

Alternate Names EC 3.5.3.15; PDI5; PDI4; HL-60 PAD; Protein-arginine deiminase type IV; PADI5; PAD4; Protein-arginine deiminase type-4; PAD; Peptidylarginine deiminase IV

Application Instructions

Application table Application Dilution

ICC/IF 1:20 - 1:50

WB 1:500 - 1:2000

Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Positive Control SKOV3

Calculated Mw 74 kDa

Observed Size 75 kDa

Properties

Form Liquid

Purification Affinity purified.

Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.

Preservative 0.02% Sodium azide

Stabilizer 50% Glycerol

Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. www.arigobio.com 1/2 Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol PADI4

Gene Full Name peptidyl arginine deiminase, type IV

Background This gene is a member of a gene family which encodes enzymes responsible for the conversion of arginine residues to citrulline residues. This gene may play a role in granulocyte and macrophage development leading to inflammation and immune response. [provided by RefSeq, Jul 2008]

Function Catalyzes the citrullination/deimination of arginine residues of proteins such as histones, thereby playing a key role in histone code and regulation of stem cell maintenance. Citrullinates histone H1 at 'Arg-54' (to form H1R54ci), histone H3 at 'Arg-2', 'Arg-8', 'Arg-17' and/or 'Arg-26' (to form H3R2ci, H3R8ci, H3R17ci, H3R26ci, respectively) and histone H4 at 'Arg-3' (to form H4R3ci). Acts as a key regulator of stem cell maintenance by mediating citrullination of histone H1: citrullination of 'Arg-54' of histone H1 (H1R54ci) results in H1 displacement from chromatin and global chromatin decondensation, thereby promoting pluripotency and stem cell maintenance. Promotes profound chromatin decondensation during the innate immune response to infection in neutrophils by mediating formation of H1R54ci. Citrullination of histone H3 prevents their methylation by CARM1 and HRMT1L2/PRMT1 and represses transcription. Citrullinates EP300/P300 at 'Arg-2142', which favors its interaction with NCOA2/GRIP1. [UniProt]

Cellular Localization Cytoplasm. Nucleus. Cytoplasmic granule. Note=Cytoplasmic granules of eosinophils and neutrophils. [UniProt]

Images

ARG40521 anti-PADI4 / PAD4 antibody ICC/IF image

Immunofluorescence: U2OS cells stained with ARG40521 anti-PADI4 / PAD4 antibody.

ARG40521 anti-PADI4 / PAD4 antibody WB image

Western blot: 25 µg of SKOV3 cell lysate stained with ARG40521 anti- PADI4 / PAD4 antibody at 1:1000 dilution.