Monoclonal Antibody to AOC3 / VAP1 - Purified

OriGene Technologies, Inc. OriGene Technologies GmbH

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AM26204PU-N Monoclonal Antibody to AOC3 / VAP1 - Purified

Alternate names: Copper amine oxidase, HPAO, Membrane primary amine oxidase, Semicarbazide- sensitive amine oxidase, Vascular adhesion protein 1 Quantity: 0.1 mg Concentration: 0.1 mg/ml Background: Vascular Adhesion Protein-1 (VAP-1) is a glycosylated homodimeric membrane protein consisting of two 90 kDa subunits connected by disulfide bonds. It contains a short N- terminal cytoplasmic tail, a single membrane-spanning domain and a large extracellular part. A soluble form of VAP-1 (sVAP-1) has been described, which presumably results from the proteolytic cleavage of membrane-bound VAP-1. Structurally VAP-1 belongs to enzymes called semicarbamizide-sensitive amine oxidases, which contain copper as a cofactor. These enzymes deaminate primary amines in a reaction producing hydrogen peroxide, aldehyde, and ammonia. VAP-1 is present in endothelial cells, smooth muscle cells, adipocytes, and in follicular dendritic cells. In endothelial cells the majority of VAP-1 is stored within intracellular granules and translocated to the surface upon inflammation where it regulates leukocyte tissue infiltration. Furthermore, the end-products formed by VAP-1 can also regulate leukocyte migration by signaling effects, have insulin-like effects in energy metabolism, and can cause vascular damage by direct cytotoxicity. Elevated sVAP-1serum levels have been described in several inflammatory diseases as well as colorectal cancer. Moreover, diminished insulin secretion appears to increase the concentration of soluble VAP-1 in plasma. Therefore, VAP-1 might be an interesting diagnostic marker as well therapeutic target for modulating inflammation. Uniprot ID: Q16853 NCBI: NP_003725.1 GeneID: 8639 Host / Isotype: Mouse / IgG1 Recommended Isotype SM10P (for use in human samples), SM20P (for use in rat samples), AM03095PU-N Controls: Clone: 174-5 Immunogen: Purified vessels from human peripheral lymph nodes (ref 1) Format: State: Liquid 0.2 µm filtered Ig fraction Purification: Protein G Buffer System: PBS Stabilizers: 0.1% bovine serum albumin Applications: Immunohistochemistry on frozen sections: Tissue sections were fixed in acetone before staining with 174-5. As positive control anti-VAP-1 mAb 2D10 was used and as negative control an isotype matched irrelevant mAb. (Ref.1). Typical starting working dilution is 1:50. Flow cytometry: Stains Ax cells stably transfected with VAP-1 cDNA.. As negative

For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. OG/20130514 1 / 2 AM26204PU-N: Monoclonal Antibody to AOC3 / VAP1 - Purified

control mock transfected cells were used.(Ref.1). Typical starting working dilution is 1:50. Functional assay: Inhibits lymphocyte infiltration in liver allograft rejection. The antibody was administered intravenously at a concentration of 2 mg/kg. A irrelevant isotype-matched antibody served as a negative control. (Ref.1). Immunoflourescence (Ref1,2). Positive control: Ax cells stably transfected with VAP-1 cDNA. (Ref. 1). Negative control: Mock transfected Ax cells. (Ref. 1). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. Specificity: This antibody detects Vascular Adhesion Protein-1 (VAP-1). Species Reactivity: Tested: Human, rat Storage: Store at 2 - 8 °C. Shelf life: one year from despatch. General Readings: 1. Martelius T, Salaspuro V, Salmi M, Krogerus L, Höckerstedt K, Jalkanen S, et al. Blockade of vascular adhesion protein-1 inhibits lymphocyte infiltration in rat liver allograft rejection. Am J Pathol. 2004 Dec;165(6):1993-2001. PubMed PMID: 15579442. 2. Autio A, Ujula T, Luoto P, Salomäki S, Jalkanen S, Roivainen A. PET imaging of inflammation and adenocarcinoma xenografts using vascular adhesion protein 1 targeting peptide 68Ga-DOTAVAP-P1: comparison with 18F-FDG. Eur J Nucl Med Mol Imaging. 2010 Oct;37(10):1918-25. doi: 10.1007/s00259-010-1497-y. Epub 2010 Jun 4. PubMed PMID: 20523988. Pictures: Immunohistochemical analysis of VAP-1 on human tonsil tissue. Staining of frozen tissue section with antibody 174-5 (AM26204PU-N). Anti-human VAP-1 staining results in vessels that are VAP-1 positive, whereas morphologically similar vessels next to positive ones can be negative.

For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. OG/20130514 2 / 2