Structure and Mechanism of a Group-I Cobalt Energy Coupling Factor Transporter
Cell Research (2017) 27:675-687. © 2017 IBCB, SIBS, CAS All rights reserved 1001-0602/17 $ 32.00 ORIGINAL ARTICLE www.nature.com/cr Structure and mechanism of a group-I cobalt energy coupling factor transporter Zhihao Bao1, 2, 3, *, Xiaofeng Qi1, 3, *, Sen Hong1, 3, Ke Xu1, 3, Fangyuan He1, Minhua Zhang1, Jiugeng Chen1, Daiyin Chao1, Wei Zhao4, Dianfan Li4, Jiawei Wang5, Peng Zhang1 1National Key Laboratory of Plant Molecular Genetics, CAS Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China; 2Shanghai Center for Plant Stress Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China; 3University of Chinese Academy of Sciences, Beijing 100049, China; 4State Key Laboratory of Molecular Biology, National Center for Protein Sciences, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chi- nese Academy of Sciences, Shanghai 200031, China; 5State Key Laboratory of Membrane Biology, School of Life Sciences, Tsing- hua University, Beijing 100084, China Energy-coupling factor (ECF) transporters are a large family of ATP-binding cassette transporters recently iden- tified in microorganisms. Responsible for micronutrient uptake from the environment, ECF transporters are mod- ular transporters composed of a membrane substrate-binding component EcfS and an ECF module consisting of an integral membrane scaffold component EcfT and two cytoplasmic ATP binding/hydrolysis components EcfA/A’. ECF transporters are classified into groups I and II. Currently, the molecular understanding of group-I ECF transport- ers is very limited, partly due to a lack of transporter complex structural information.
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