3 minutes 6 minutes
9 minutes 12 minutes
Time lapse experiment with chick DRG neuron, growth cone of an axon after 3, 6, 9, 12 minutes. Green: Actin, YFP Courtesy of Kazumasa Ohashi, Graduate School of Life Sciences, Tohoku University, Japan Imaging Unlocked!
Leica’s NEW AF6000 Advanced Fluorescence software package significantly simplifies experiments in time lapse, multi-channel fluorescence, and deconvolution. This advanced fluorescence software, coupled with a Leica microscope and digital camera, provides an intuitive interface for defining experiments and performing data analysis instead of offering an endless toolbar. Leica AF6000 simplifies training, ensures proper imaging parameters, and increases efficiency.
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Intelligent Automation Leica Microsystems, Inc., 2345 Waukegan Road, Bannockburn, IL 60015 Tel. 847-405-0123, 800-248-0123, Fax 847-405-0164, In Canada call 800-205-3422 ©2006 Leica Microsystems Inc., BNA#542 santa cruz biotechnology, inc. The Power to Question fusion protein tag antibodies
SEE OUR WEB SITE AND NEW 2007 CATALOG FEATURING: 5512 new monoclonal antibodies
2472 new polyclonal Santa Cruz Biotechnology, Inc. offers a wide range of antibodies to fusion protein tags. antibodies Select HA, GST, His, GFP, GST, c-Myc and OctA tag antibodies are featured here. See our web site www.scbt.com for our complete fusion protein tag antibody product 5082 new siRNAs line. OctA-Probe fusion protein tag antibodies are raised against an eight amino acid epitope tag identical to the FLAG® peptide sequence.
fusion protein tag antibodies PRODUCT CAT# ISOTYPE APPLICATIONS GFP (FL) sc-8334 rabbit IgG WB, IP, IF GFP (T-19) sc-5384 goat IgG WB, IF
GFP (B-2) sc-9996 mouse IgG2a WB, IP, IF His-probe (H-15) sc-803 rabbit IgG WB, IP
His-probe (H-3) sc-8036 mouse IgG1 WB, IP
c-Myc (9E10) sc-40 mouse IgG1 WB, IP, IF, IHC(P), FCM
c-Myc (9E11) sc-47694 mouse IgG2a WB, IP, IF, IHC(P) c-Myc (A-14) sc-789 rabbit IgG WB, IP, IF, FCM GST (Z-5) sc-459 rabbit IgG WB, IP
GST (B-14) sc-138 mouse IgG1 WB, IP
HA-probe (F-7) sc-7392 mouse IgG2a WB, IP, IF HA-probe (Y-11) sc-805 rabbit IgG WB, IP, IF OctA-Probe (D-8) sc-807 rabbit IgG WB, IP, IF, IHC(P)
OctA-Probe (H-6) sc-7787 mouse IgG1 WB, IP, IF, IHC(P) GAL4 (DBD) (N-19) sc-729 rabbit IgG WB, IP GAL4-TA (768) sc-429 rabbit IgG WB, IP
HEADQUARTERS A B TEL: 831.457.3800 A B C 81 K – 132 K TOLL FREE: 1.800.457.3801 48 K – HA fusion 90 K proteins FAX: 831.457.3801 55 K < OctA (FLAG®)- EMAIL: [email protected] 43 K tagged fusion 35 K – protein 28 K – 34 K EUROPEAN SUPPORT 19 K – TEL: +49.6221.4503.0 TOLL FREE: +00800.4573.8000
Immunofl uorescence staining of methanol-fi xed HA-probe (F-7): sc-7392. OctA-Probe (D-8)-G: sc-807-G. FAX: +49.6221.4503.45 COS cells transfected with c-Myc fusion protein Western blot analysis of HA- Western blot analysis of OctA EMAIL: [email protected] showing cytoplasmic staining. Antibodies tested tagged fusion proteins showing (FLAG®)-tagged fusion protein include c-Myc (A-14): sc-789 (A) and c-Myc (9E10): N-terminal HA-tagged JNK2 (A) from Sigma. sc-40 (B). and JNK1 (C) and C-terminal HA-tagged Daxx (B). ASIAN SUPPORT S. KOREA TOLL FREE: 00798.1.1.002.0297 JAPAN TOLL FREE: (010) 800.40402026 CHINA TOLL FREE: 10.800.711.0752 or 10.800.110.0694 EMAIL: [email protected]
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LightCycler® 480 Real-Time PCR System Hunt for new targets: High-Resolution Melting Meets High-Performance PCR
The LightCycler® 480 System offers new tools for mutation scanning based on high-resolution melting (HRM). This highly sensitive post-PCR method enables high-throughput SNP screening at a much lower cost. ■ Discover genetic variation — fast: Choose the first fully integrated PCR/HRM solution for 96- and 384-well plates.
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■ Avoid unnecessary sequencing: Streamline your research by taking LightCycler® 480 Gene Scanning Software reveals advantage of a highly robust and convenient mutation scanning method. differences between wild types and variants by grouping of HRM curves. A fragment of the human Take your PCR to new heights. LPLH3 gene was amplified from blood using the Learn more at www.lightcycler480.com LightCycler® 480 High Resolution Melting Master. Difference curve analysis enables differentiation between wild-type (green), homozygous G to T mutant (red), and heterozygous (blue) samples.
For general laboratory use. Not for use in diagnostic procedures. Purchase of this product is accompanied by a limited license to use it in the PCR process, including homogeneous PCR methods described in U.S. Patents Nos. 5,994,056, 6,171,785, 6,569,627 and corresponding patents outside the United States, for life science research in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by the up-front license fee, i.e., an authorized thermal cycler. No real-time apparatus or system patent rights or any other patent rights owned by Applera Corporation are conveyed expressly, by implication or by estoppel. No rights for any other application, including any in vitro diagnostic application, are conveyed expressly, by implication or by estoppel under U.S. Patents Nos. 6,174,670 and 6,245,514 and corresponding patent claims outside the United States, or any other patents owned by Roche Molecular Systems, Inc. and F. Hoffmann-La Roche Ltd, claiming real-time amplification and Roche Diagnostics GmbH detection methods. The product is covered in-part by US 5,871,908, co-exclusively licensed from Evotec OAI AG. Parts of the Software used for the LightCycler® 480 System are licensed from Idaho Technology Inc., Salt Lake City, UT, USA Roche Applied Science LIGHTCYCLER is a trademark of Roche. Other brands or product names are trademarks of their respective holders. © 2007 Roche Diagnostics GmbH. All rights reserved. 68298 Mannheim, Germany
Subject Coverage Antibodies Bioinformatics/Genomics Now you can see results of the finest methods in action with new online movies in Cell Biology CSH Protocols. Chromatography The November issue of CSH Protocols features methods for tracking RNA and Computational Biology protein molecules in both cells and organisms, and introduces new multimedia content made possible by online presentation: movies showing examples of these DNA Delivery/Gene Transfer techniques in action. Electrophoresis Read—and see—these newly featured protocols online at www.cshprotocols.org: Genetics • Photoactivation-Based Labeling and In Vivo Tracking of RNA Molecules in the Nucleus High Throughput Analysis • Selection of Appropriate Imaging Equipment and Methodology for Live Cell Imaging in Imaging/Microscopy Drosophila Immunology CSH Protocols offers cutting edge and classic protocols in molecular and cell biology Laboratory Organisms from laboratories worldwide, along with methods from Cold Spring Harbor Molecular Biology Laboratory’s renowned manuals and on-site courses. Free trials and discounted subscriptions are available for a limited time. Neuroscience CSH Protocols is created by Cold Spring Harbor Laboratory Press in association Plant Biology with HighWire Press of Stanford University. Polymerase Chain Reaction (PCR) Cold Spring Harbor Protocols • Executive Editor: Dr. Michael Ronemus Proteins and Proteomics ISSN 1559-6095 / online, monthly • Available exclusively via institutional site license RNA Interference (RNAi)/siRNA Stem Cells Transgenic Technology
For pricing information or to request a free trial visit the website or: Phone:1-800-843-4388 (Continental US and Canada) or 516-422-4100 (all other locations) FAX: 516-422-4097 E-mail: [email protected] Write: Cold Spring Harbor Laboratory Press, 500 Sunnyside Blvd., Woodbury, NY 11797-2924