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Relationship Between the Conformation of the Cyclopeptides Isolated from the Fungus Amanita Phalloides (Vaill. Ex Fr.) Secr. and Its Toxicity
Molecules 2000, 5 489 Relationship Between the Conformation of the Cyclopeptides Isolated from the Fungus Amanita Phalloides (Vaill. Ex Fr.) Secr. and Its Toxicity M.E. Battista, A.A. Vitale and A.B. Pomilio PROPLAME-CONICET, Departamento de Química Orgánica, Facultad de Ciencias Exactas y Natu- rales, Universidad de Buenos Aires, Pabellón 2, Ciudad Universitaria, 1428 Buenos Aires, Argentina E-mail: [email protected] Abstract: The electronic structures and conformational studies of the cyclopeptides, O- methyl-α-amanitin, phalloidin and antamanide, were obtained from molecular parameters on the basis of semiempiric and ab initio methods. Introduction During this century Amanita phalloides - the most toxic fungus known up to now - has been studied from different points of view. This basidiomycete biosynthesizes mono- and bicyclic peptides com- posed of rare amino acids. In order to determine the structure/activity relationships chemical modifica- tions were carried out and the properties of these compounds were evaluated. These results were con- firmed by studying the conformations of three selected compounds representative of the major groups of the macroconstituents of this fungus. Experimental Hyperchem package (HyperCube, version 5.2) was used for semiempirical studies, the molecular geometry being optimized by STO-631G. Net charges were calculated with HyperCube PM3 and the Polack-Ribiere algoritm. GAUSSIAN 98 was used for ab initio studies. Results and Discussion We were interested in obtaining information on the conformations that the cyclic peptides may adopt and about the potential energy maps in order to locate the regions related to the binding to pro- tein molecules, such as F-actin and RNA-polymerase. -
Collagen and Elastin Fibres
J Clin Pathol: first published as 10.1136/jcp.s3-12.1.49 on 1 January 1978. Downloaded from J. clin. Path., 31, Suppl. (Roy. Coll. Path.), 12, 49-58 Collagen and elastin fibres A. J. BAILEY From the Agricultural Research Council, Meat Research Institute, Langford, Bristol Although an understanding of the intracellular native collagen was generated from type I pro- biosynthesis of both collagen and elastin is of collagen. Whether this means that the two pro- considerable importance it is the subsequent extra- collagens are converted by different enzyme systems cellular changes involving fibrogenesis and cross- and the type III enzyme was deficient in these linking that ensure that these proteins ultimately fibroblast cultures, or that the processing of pro become the major supporting tissues of the body. type III is extremely slow, is not known. The latter This paper summarises the formation and stability proposal is consistent with the higher proportion of collagen and elastin fibres. of soluble pro type III extractable from tissue (Lenaers and Lapiere, 1975; Timpl et al., 1975). Collagen Basement membrane collagens, on the other hand, do not form fibres and this property may be The non-helical regions at the ends of the triple due to the retention of the non-helical extension helix of procollagen probably provide a number of peptides (Kefalides, 1973). In-vivo biosynthetic different intracellular functions-that is, initiating studies showing the absence of any extension peptide rapid formation of the triple helix; inhibiting intra- removal support this (Minor et al., 1976), but other cellular fibrillogenesis; and facilitating transmem- workers have reported that there is some cleavage brane movement. -
Synthetic Polynucleotides Synthetische Polynukleotide Polynucleotides Synthetiques
Europäisches Patentamt *EP000960192B1* (19) European Patent Office Office européen des brevets (11) EP 0 960 192 B1 (12) EUROPEAN PATENT SPECIFICATION (45) Date of publication and mention (51) Int Cl.7: C12N 9/02, C12N 15/53, of the grant of the patent: A61K 38/43, C12N 9/06 09.11.2005 Bulletin 2005/45 (86) International application number: (21) Application number: 97933592.4 PCT/AU1997/000505 (22) Date of filing: 11.08.1997 (87) International publication number: WO 1998/006830 (19.02.1998 Gazette 1998/07) (54) SYNTHETIC POLYNUCLEOTIDES SYNTHETISCHE POLYNUKLEOTIDE POLYNUCLEOTIDES SYNTHETIQUES (84) Designated Contracting States: • SHARP P M ET AL: "The codon Adaptation AT BE CH DE DK ES FI FR GB GR IE IT LI LU MC Index--a measure of directional synonymous NL PT SE codon usage bias, and its potential applications." NUCLEIC ACIDS RESEARCH. (30) Priority: 09.08.1996 AU PO156596 ENGLAND 11 FEB 1987, vol. 15, no. 3, 11 February 1987 (1987-02-11), pages 1281-1295, (43) Date of publication of application: XP001122356 ISSN: 0305-1048 01.12.1999 Bulletin 1999/48 • DATABASE SWISSPROT [Online] 1 December 1992 (1992-12-01) MARIANI T.J. ET AL.: (60) Divisional application: "Protein-lysine 6-oxidase precursor (EC 05000327.6 1.4.3.13) (Lysyl oxidase)." Database accession no. P28300 XP002229125 (73) Proprietor: THE UNIVERSITY OF SYDNEY • DATABASE EMBL [Online] EBI; 16 May 1992 Sydney, New South Wales 2006 (AU) (1992-05-16) MARIANI T.J. ET AL.: "Human lysyl oxidase (LOX) mRNA, complete cds." Database (72) Inventor: WEISS, Anthony, Steven accession no. M94054 XP002229126 Randwick, NSW 2031 (AU) • DATABASE EMBL [Online] EBI; 26 November 1993 (1993-11-26) HAMALAINEN E.R. -
Organic Anion Transporter 2–Mediated Hepatic Uptake
Supplemental material to this article can be found at: http://jpet.aspetjournals.org/content/suppl/2018/08/22/jpet.118.252049.DC1 1521-0103/367/2/322–334$35.00 https://doi.org/10.1124/jpet.118.252049 THE JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS J Pharmacol Exp Ther 367:322–334, November 2018 Copyright ª 2018 by The American Society for Pharmacology and Experimental Therapeutics Organic Anion Transporter 2–Mediated Hepatic Uptake Contributes to the Clearance of High-Permeability–Low- Molecular-Weight Acid and Zwitterion Drugs: Evaluation Using 25 Drugs s Emi Kimoto, Sumathy Mathialagan, Laurie Tylaska, Mark Niosi, Jian Lin, Anthony A. Carlo, David A. Tess, and Manthena V. S. Varma Medicine Design, Worldwide Research and Development, Pfizer Inc., Groton, Connecticut Downloaded from Received July 12, 2018; accepted August 15, 2018 ABSTRACT High-permeability–low-molecular-weight acids/zwitterions [i.e., drugs (21 of 25) showed active uptake by plated human hepa- extended clearance classification system class 1A (ECCS 1A) tocytes, with rifamycin SV (pan-transporter inhibitor) reducing drugs] are considered to be cleared by metabolism with a the uptake by about 25%–95%. Metabolic turnover was esti- jpet.aspetjournals.org minimal role of membrane transporters in their hepatic clearance. mated for 19 drugs after a few showed no measurable substrate However, a marked disconnect in the in vitro-in vivo (IVIV) depletion in liver microsomal incubations. IVIV extrapolation translation of hepatic clearance is often noted for these drugs. using in vitro data was evaluated to project human hepatic Metabolic rates measured using human liver microsomes and clearance of OAT2-alone substrates considering 1) uptake trans- primary hepatocytes tend to underpredict. -
Unique Amalgamation of Primary and Secondary Structural Elements
Unique amalgamation of primary and secondary PNAS PLUS structural elements transform peptaibols into potent bioactive cell-penetrating peptides Lin Dua,b,1, April L. Risingerc,d,1, Carter A. Mitchella,b,1, Jianlan Youa,b, Blake W. Stampse, Ning Pana, Jarrod B. Kinga,b, Jean C. Bopassaf, Susan I. V. Judgeg,h, Zhibo Yanga, Bradley S. Stevensona,e, and Robert H. Cichewicza,b,2 aDepartment of Chemistry and Biochemistry, Stephenson Life Sciences Research Center, University of Oklahoma, Norman, OK 73019-5251; bNatural Products Discovery Group, Institute for Natural Products Applications and Research Technologies, University of Oklahoma, Norman, OK 73019-5251; cDepartment of Pharmacology, University of Texas Health Science Center, San Antonio, TX 78229; dCancer Therapy & Research Center, University of Texas Health Science Center, San Antonio, TX 78229; eDepartment of Microbiology and Plant Biology, University of Oklahoma, Norman, OK 73019-5251; fDepartment of Physiology, School of Medicine, University of Texas Health Science Center, San Antonio, TX 78229; gDepartment of Biochemistry, High Throughput Screening Facility, Center for Innovative Drug Discovery, University of Texas Health Science Center, San Antonio, TX 78229; and hCytoBioscience Incorporated, San Antonio, TX 78229 Edited by Jerrold Meinwald, Cornell University, Ithaca, NY, and approved September 18, 2017 (received for review May 7, 2017) Mass-spectrometry-based metabolomics and molecular phylogeny organisms to evolve new biological functions from existing molec- data were used to identify a metabolically prolific strain of Tolypo- ular scaffolds. The gonanes (perhydrocyclopenta[a]phenanthrenes) cladium that was obtained from a deep-water Great Lakes sediment are a classic example of chemical repurposing with members of this sample. -
Acute Phase Proteins and Biomarkers for Health in Chickens Emily
O'Reilly, Emily (2016) Acute phase proteins and biomarkers for health in chickens. PhD thesis http://theses.gla.ac.uk/7428/ Copyright and moral rights for this thesis are retained by the author A copy can be downloaded for personal non-commercial research or study, without prior permission or charge This thesis cannot be reproduced or quoted extensively from without first obtaining permission in writing from the Author The content must not be changed in any way or sold commercially in any format or medium without the formal permission of the Author When referring to this work, full bibliographic details including the author, title, awarding institution and date of the thesis must be given. Glasgow Theses Service http://theses.gla.ac.uk/ [email protected] 1 Acute phase proteins and biomarkers for health in chickens Emily Louise O’Reilly B.Sc. (Hon), BVMS, M.Sc. Submitted in fulfilment of the requirements for the degree of Doctor of Philosophy (Ph.D.) Institute of Biodiversity, Animal Health and Comparative Medicine College of Medical, Veterinary and Life Sciences University of Glasgow February 2016 © E. L. O’Reilly, 2016 2 Abstract Acute phase proteins (APPs) are proteins synthesised predominantly in the liver, whose plasma concentrations increase (positive APP) or decrease (negative APP) as a result of infection, inflammation, trauma and tissue injury. They also change as a result of the introduction of immunogens such as bacterial lipopolysaccharide (LPS), turpentine and vaccination. While publications on APPs in chickens are numerous, the limited availability of anti-sera and commercial ELISAs has resulted in a lot of information on only a few APPs. -
Toxic Fungi of Western North America
Toxic Fungi of Western North America by Thomas J. Duffy, MD Published by MykoWeb (www.mykoweb.com) March, 2008 (Web) August, 2008 (PDF) 2 Toxic Fungi of Western North America Copyright © 2008 by Thomas J. Duffy & Michael G. Wood Toxic Fungi of Western North America 3 Contents Introductory Material ........................................................................................... 7 Dedication ............................................................................................................... 7 Preface .................................................................................................................... 7 Acknowledgements ................................................................................................. 7 An Introduction to Mushrooms & Mushroom Poisoning .............................. 9 Introduction and collection of specimens .............................................................. 9 General overview of mushroom poisonings ......................................................... 10 Ecology and general anatomy of fungi ................................................................ 11 Description and habitat of Amanita phalloides and Amanita ocreata .............. 14 History of Amanita ocreata and Amanita phalloides in the West ..................... 18 The classical history of Amanita phalloides and related species ....................... 20 Mushroom poisoning case registry ...................................................................... 21 “Look-Alike” mushrooms ..................................................................................... -
Methods, Compounds, Compositions and Vehicles for Delivering 3-Amino-1-Propanesulfonic Acid
(19) TZZ _ T (11) EP 2 862 581 A2 (12) EUROPEAN PATENT APPLICATION (43) Date of publication: (51) Int Cl.: 22.04.2015 Bulletin 2015/17 A61K 47/48 (2006.01) C07K 5/06 (2006.01) C07K 5/08 (2006.01) C07C 309/15 (2006.01) (2006.01) (2006.01) (21) Application number: 14200552.9 C07D 207/16 C07D 209/20 C07D 217/24 (2006.01) C07D 233/64 (2006.01) (2006.01) (2006.01) (22) Date of filing: 12.10.2007 C07D 291/02 C07D 333/24 C12P 11/00 (2006.01) A61K 38/07 (2006.01) A61K 38/08 (2006.01) A61P 25/28 (2006.01) (84) Designated Contracting States: (72) Inventor: The designation of the inventor has not AT BE BG CH CY CZ DE DK EE ES FI FR GB GR yet been filed HU IE IS IT LI LT LU LV MC MT NL PL PT RO SE SI SK TR (74) Representative: Hoffmann Eitle Patent- und Rechtsanwälte PartmbB (30) Priority: 12.10.2006 US 851039 P Arabellastraße 30 12.04.2007 US 911459 P 81925 München (DE) (62) Document number(s) of the earlier application(s) in Remarks: accordance with Art. 76 EPC: This application was filed on 30-12-2014 as a 07875176.5 / 2 089 417 divisional application to the application mentioned under INID code 62. (71) Applicant: BHI Limited Partnership Laval, QC H7V 4A7 (CA) (54) Methods, compounds, compositions and vehicles for delivering 3- amino-1-propanesulfonic acid (57) The invention relates to methods, compounds, that will yield or generate 3APS, either in vitro or in vivo. -
Erature and Shear Thinning Behaviour, Suggesting That Ovomucin Can Be Used As a Thickener and Stabilizer in Various Applications
University of Alberta N-glycosylation and gelling properties of ovomucin from egg white by Marina Offengenden A thesis submitted to the Faculty of Graduate Studies and Research in partial fulfillment of the requirements for the degree of Master of Science in Food Science and Technology Department of Agricultural, Food and Nutritional Science ©Marina Offengenden Fall 2011 Edmonton, Alberta Permission is hereby granted to the University of Alberta Libraries to reproduce single copies of this thesis and to lend or sell such copies for private, scholarly or scientific research purposes only. Where the thesis is converted to, or otherwise made available in digital form, the University of Alberta will advise potential users of the thesis of these terms. The author reserves all other publication and other rights in association with the copyright in the thesis and, except as herein before provided, neither the thesis nor any substantial portion thereof may be printed or otherwise reproduced in any material form whatsoever without the author's prior written permission. Dedication To Ron, my shining sun on cloudy days Abstract Ovomucin is a bioactive egg white glycoprotein responsible for its gel-like properties and is believed to be involved in egg white thinning, a natural process that occurs during storage. Ovomucin is composed of two subunits: a carbohydrate-rich β-ovomucin and a carbohydrate-poor α-ovomucin. N- glycosylation of ovomucin was studied by nano LC ESI-MS, MS/MS and MALDI MS. Both proteins were N-glycosylated and site-occupancy of 18 potential N-glycosylation sites in α-ovomucin and two sites in β-ovomucin was determined. -
Alamethicin (A4665)
Alamethicin from Trichoderma viride Catalog Number A4665 Storage Temperature 2–8 °C CAS RN 27061-78-5 Preparation Instructions Synonym: Antibiotic U-22324 Alamethicin is soluble in ethanol at 100 mg/ml and in methanol at 20 mg/ml. Product Description Alamethicin is a 20-amino acid channel-forming peptide Storage/Stability antibiotic (~2 kDa) isolated from the fungus Store desiccated and protected from light at 2–8 °C. Trichoderma viride. It consists of several isoforms, for Under these conditions the product remains active for which structural information has been published.1-5 This 3 years. product is a mixture of alamethicin isoforms. References Alamethicin catalyzes the exchange of protons for 1. Data for Biochemical Research, Dawson, R.M.C., monovalent cations with little difference in affinities1,6 et al., eds., Oxford Science Publications (Oxford, and has the ability to transport cations through UK: 1986), 3rd ed. biological and artificial lipid membranes. Its function is 2. Dictionary of Organic Compounds, Buckingham, similar to Gramicidin A in that it forms pores or J., and MacDonald, F.M., Eds., Chapman & Hall channels in the membrane in a voltage-dependent (New York, NY: 1995), 5th ed., #A-10059. manner.6 It also causes hemolysis of erythrocytes1 and 3. Pandey, R.C., et al., High resolution and field because it decreases the surface tension of water, it desorption mass spectrometry studies and revised may have use as a detergent. A solution containing structures of alamethicins I and II. J. Am. Chem. 100 mg/ml in deionized water has a surface tension of Soc., 99, 8469-8483 (1977). -
Synthetic Peptides: Design, Structure and Biological Function
Louisiana State University LSU Digital Commons LSU Historical Dissertations and Theses Graduate School 2001 Synthetic Peptides: Design, Structure and Biological Function. Lars Gustav johan Hammarstrom Louisiana State University and Agricultural & Mechanical College Follow this and additional works at: https://digitalcommons.lsu.edu/gradschool_disstheses Recommended Citation Hammarstrom, Lars Gustav johan, "Synthetic Peptides: Design, Structure and Biological Function." (2001). LSU Historical Dissertations and Theses. 289. https://digitalcommons.lsu.edu/gradschool_disstheses/289 This Dissertation is brought to you for free and open access by the Graduate School at LSU Digital Commons. It has been accepted for inclusion in LSU Historical Dissertations and Theses by an authorized administrator of LSU Digital Commons. For more information, please contact [email protected]. INFORMATION TO USERS This manuscript has been reproduced from the microfilm master. UMI films the text directly from the original or copy submitted. Thus, some thesis and dissertation copies are in typewriter face, while others may be from any type of computer printer. The quality of this reproduction is dependent upon the quality of the copy submitted. Broken or indistinct print, colored or poor quality illustrations and photographs, print bleedthrough, substandard margins, and improper alignment can adversely affect reproduction. In the unlikely event that the author did not send UMI a complete manuscript and there are missing pages, these will be noted. Also, if unauthorized copyright material had to be removed, a note will indicate the deletion. Oversize materials (e.g., maps, drawings, charts) are reproduced by sectioning the original, beginning at the upper left-hand comer and continuing from left to right in equal sections with small overlaps. -
Anticancer and Immunomodulatory Activity of Egg Proteins and Peptides: a Review
Anticancer and immunomodulatory activity of egg proteins and peptides: a review J. H. Lee and H.-D. Paik1 Department of Food Science and Biotechnology of Animal Resources, Konkuk University, Seoul 05029, Korea ABSTRACT Eggs are widely recognized as a highly mortality worldwide, and therefore research aimed at nutritious food source that offer specific health benefits developing new treatments for cancer immunotherapy for humans. Eggs contain all of the proteins, lipids, vi- is of great interest. The present review focuses primar- tamins, minerals, and growth factors necessary for em- ily on the anticancer and immunomodulatory activities bryonic development. In particular, egg white and yolk of egg proteins and their peptides and provides some proteins are considered functional food substances be- insight into their underlying mechanisms of action. A cause they possess biological activities such as antimi- number of egg proteins and peptides have been reported crobial, antioxidant, metal-chelating, antihypertensive, to induce apoptosis in cancer cells, protect against anticancer, and immunomodulatory activities. Peptides DNA damage, decrease the invasion ability of cancer produced via processes such as enzymatic hydroly- cells, and exhibit cytotoxic and antimutagenic activity sis, fermentation by microorganisms, and some chemi- in various cancer cell lines. Furthermore, egg proteins cal and physical treatments of egg proteins have been and peptides can stimulate or suppress pro- or anti- shown to enhance the functional properties and solu- inflammatory cytokines, as well as affect the production bility of these peptides. Peptide activity is strongly re- of inflammatory mediators in a variety of cell lines. In lated to amino acid sequence, composition, and length.