New Insights Into Testicular Germ Cell Tumorigenesis from Gene Expression Profiling1
[CANCER RESEARCH 62, 2359–2364, April 15, 2002] New Insights into Testicular Germ Cell Tumorigenesis from Gene Expression Profiling1 Rolf I. Skotheim, Outi Monni, Spyro Mousses, Sophie D. Fosså, Olli-P. Kallioniemi, Ragnhild A. Lothe,2 and Anne Kallioniemi Department of Genetics, Institute for Cancer Research [R. I. S., R. A. L.] and Department for Oncology and Radiotherapy [S. D. F.], The Norwegian Radium Hospital, N-0310 Oslo, Norway; Biomedicum Biochip Center, Biomedicum Helsinki, FIN-00290 Helsinki, Finland [O. M.]; Cancer Genetics Branch, National Human Genome Research Institute, NIH, Bethesda, Maryland 20892 [O. M., S. M., O-P. K., A. K.]; and Laboratory of Cancer Genetics, Institute of Medical Technology, University of Tampere and Tampere University Hospital, FIN-33520 Tampere, Finland [A. K.] ABSTRACT teristic of virtually all TGCTs (4, 5). In addition, specific gains and losses from several other chromosomal regions have been described. 3 We have shown recently that about half of the human TGCTs reveal Although molecular studies have shown some genes to be altered at DNA copy number increases affecting two distinct regions on chromosome the DNA and/or expression levels in a limited number of TGCTs, the arm 17q. To identify potential target genes with elevated expressions attributable to the extra copies, we constructed a cDNA microarray target genes reflecting the nonrandom chromosomal aberrations re- containing 636 genes and expressed sequence tags from chromosome 17. main unknown (for a review of TGCT genetics, see Refs. 6 and 7). The expression patterns of 14 TGCTs, 1 carcinoma in situ, and 3 normal We have demonstrated recently by a genome-wide copy number testis samples were examined, all with known chromosome 17 copy num- analysis using CGH that sequences on chromosome arm 17q are bers.
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