Precast Gels, 10 % -Tricine Gel (DPG054) This product is for research use only and is not intended for diagnostic use.

PRODUCT INFORMATION

Size 10 gels

Storage Storage conditions: up to 1 year at 4°C, Never store gels at temperatures lower than +4° C

Intended Use Instructions for Using Precast Gels Cat#: DPG054; DPG055; DPG056 Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) is used to determine the approximate molecular weights of proteins with reference to the mobility of standard proteins on the same gel. Precast polyacrylamide gels for SDS-PAGE that have a long shelf-life and short run time and yield outstanding resolution. Gradient gels provide the appropriate gel pore size making molecular weight estimation more accurate by sharpening stained protein bands.

General Description Formulation: Precast 10 % Tris--Gel polyacrylamide gels for SDS-PAGE Separation Range (kDa): 8--150 kDa Sufficient For: DPG054-10:10 samples per gel, 1,0 mm/1,5 mm DPG054-12:12 samples per gel, 1,0 mm/1,5 mm DPG054-15:15 samples per gel, 1,0 mm/1,5 mm

Principles of Testing Migration Table by Gel Percentage

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Tel: 1-631-624-4882 Fax: 1-631-938-8221 1 © Creative Diagnostics All Rights Reserved Reagents And Materials Specifications of Tris- Gels Provided Cassette dimensions: 10cm x 10cm x 7mm Gel dimensions: 8cm x 8.8cm x 1mm

Materials Required But Not The following precast gels are made in disposable cassettes with a format of 10 cm x 10 cm Supplied fitting well into most of the available mini-gel electrophoresis cells, like NOVEX, Serva, Hoefer, Biometra, Scie-Plas, etc. If in doubt please ask for compatibility.

Reagent Preparation Electrophoresis Protocol

45-1 Ramsey Road, Shirley, NY 11967, USA Email: [email protected]

Tel: 1-631-624-4882 Fax: 1-631-938-8221 2 © Creative Diagnostics All Rights Reserved Assay Procedure Handling Recommendations for Precast Gels 1. Open gel-pouch with scissors, take out the gel-cassette. 2. Rinse the cassette with DI-water. 3. Remove the tape strip at the lower part of the cassette. 4. Remove the comb with a quick, vertical stroke. 5. Dependent on the requirements of cells used, look for a tight fit of the cassette. 6. Using a Pasteur-pipette, wash the wells gently with Running-buffer and fill them up completely, avoiding airbubbles. 7. Positioning of samples should be at the bottom of each well, avoid swirls. 8. Fill the electrophoresis cell with Running-buffer, on Cathode and Anode sides. 9. Close the cell and connect the cables to the power-supply. 10. Select the appropriate running conditions and start electrophoresis. 11. After elapsed runtime, switch instrument off, remove power cables and take the cassette(s) out of the cell. 12. Open the cassette with a gel-knife, a strong spatula or by using the gel-comb to crack the ultrasonic welding between the front and rear cassette plate. The cassette should be positioned horizontally on the table with the curved top plate down. Remove the front plate. 13. Cut the lower portion of the gel directly above the power-slot and the comb-wells with the gel- knife. 14. Now take the gel in the hand and turn it 180 degrees upside down. Carefully take one edge of the gel with the gel-knife and shake the plate slightly. 15. Gravity will help the gel to be removed from plate very easily, falling in a tray. Note: Never try to scratch the gel from the plate with any tool. 16. Now you may continue with your appropriate work procedures: fixing, staining, blotting etc.

45-1 Ramsey Road, Shirley, NY 11967, USA Email: [email protected]

Tel: 1-631-624-4882 Fax: 1-631-938-8221 3 © Creative Diagnostics All Rights Reserved Introduction Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) is used to determine the approximate molecular weights of proteins with reference to the mobility of standard proteins on the same gel. Precast polyacrylamide gels for SDS-PAGE that have a long shelf-life and short run time and yield outstanding resolution. Gradient gels provide the appropriate gel pore size making molecular weight estimation more accurate by sharpening stained protein bands.

Keywords precast, protein, sds, minigel, mini, gel, Precast Gels, Tris-Tricine/Mops Gels polyacrylamide gels

45-1 Ramsey Road, Shirley, NY 11967, USA Email: [email protected]

Tel: 1-631-624-4882 Fax: 1-631-938-8221 4 © Creative Diagnostics All Rights Reserved