AquaBiomics eDNA Analysis Report
About this report Generated on: Fri Mar 19 20:36:25 2021 This report summarizes an analysis of eDNA (environmental DNA) in an aquarium water sample. Universal genetic markers were amplified from Eukaryotic DNA in the sample, and thousands of individual DNA molecules from this mixture were sequenced. Each sequence was then compared with public DNA databases to identify its origin. (You can read more about this method here).
This report summarizes the kinds of organisms detected in this sample, with a special focus on pathogens and parasites of concern in the saltwater aquarium industry and hobby.
Information about the sample
Sample ID p347
Sample Name 120G_mixed_01
Sample Date 2/21/2021 11:40
Number of Sequences Analyzed 3453
Parasites None of the DNA sequences from this sample matched known parasites. View the full table
Percent of Group Description eDNA
Amyloodinium Dinoflagellate parasites responsible for the fish disease Marine Velvet 0
Cryptocaryon Ciliate parasites responsible for the fish disease Ich 0
Brooklynella Ciliate parasites responsible for the fish disease Brook 0
Uronema Ciliate parasites responsible for a fish disease called by the same 0 name
Phestilla Montipora-eating Nudibranchs 0 Percent of Group Description eDNA
Amakusaplana Acropora-eating Flatworms 0
Ancyrocephalidae Flatworms often called Flukes that parasitize fish 0
Gyrodactylidae Flatworms often called Flukes that parasitize fish 0
Capsalidae Flatworms often called Flukes that parasitize fish 0
Turbonilla Snails in the family Pyramidellidae that parasitize giant clams 0
Perkinsus Apicomplexan parasites of clams and other bivalves 0
Trichodina A ciliated protozoan parasite of fish 0
Other Animal Pests None of the DNA sequences from this sample matched known animal pests. View the full table
Group Description Percent of eDNA
Aiptasia The glass anemone, a ubiquitous pest 0
Thylacodes Vermetid snails 0
Asterina A group of sea stars that includes coral predators 0
Eukaryotic Biodiversity
Diversity (Number of different types) 60
Number of Phyla 18
Number of Classes 23
This pie chart gives an overview of Eukaryotic Biodiversity in the sample. Each slice represents a different Class, and the size of the slice is proportional to the number of different types in that class. Unidentified sequences lacking any match in public databases are not shown.
Complete list of Eukaryotes identified These tables, arranged by Class, list all Eukaryotic species that were detected in the sample and identified based on comparison with public DNA sequence databases. DNA sequences that lacked any matches in public datases could not be identified, and are not shown here. “Type” is an arbitrary number assigned to each unique sequence found in the database.
Read more…
Actinopterygii
Type Percent of eDNA Family Name
544 0.579 Actinopterygii Neolamprologus brichardi
Note: This category includes all fish DNA in the sample, since the marker used here does not distinguish between fish species.
Agaricomycetes
Type Percent of eDNA Family Name Type Percent of eDNA Family Name
1018 0.203 Atheliaceae Trametes versicolor
Anthozoa
Type Percent of eDNA Family Name
494 0.666 Scleractinia Blastomussa vivida
573 0.550 Alcyonacea Xenia sp.
774 0.348 Alcyonacea Heliopora sp.
1358 0.058 Alcyonacea Heliopora sp.
1359 0.058 Alcyonacea Xenia sp.
1360 0.058 Scleractinia Blastomussa vivida
1393 0.029 Scleractinia Blastomussa vivida
Bacillariophyceae
Type Percent of eDNA Family Name
29 0.376 Bacillariophyceae uncultured eukaryote
Calcarea
Type Percent of eDNA Family Name
16 13.322 Leucosolenida Grantiopsis heroni
682 0.290 Leucosolenida Sycon ciliatum
Chromerida
Type Percent of eDNA Family Name
773 0.348 Chromerida Chromera velia
Chytridiomycetes
Type Percent of eDNA Family Name Type Percent of eDNA Family Name
510 0.637 Lobulomycetaceae uncultured Chytridiomycota
Dinophyceae
Type Percent of eDNA Family Name
50 7.964 Gonyaulacales Ostreopsis cf.
243 0.956 Gymnodiniphycidae Amphidinium sp.
246 0.434 Gymnodiniphycidae Moestrupia sp.
1238 0.116 Gymnodiniphycidae Moestrupia sp.
Gastropoda
Type Percent of eDNA Family Name
103 0.348 Vetigastropoda Turbo cornutus
Hydrozoa
Type Percent of eDNA Family Name
964 0.232 Anthoathecata Stauridiosarsia gemmifera
Intramacronucleata
Type Percent of eDNA Family Name
25 25.051 Oligotrichia uncultured eukaryote
380 0.376 Oligohymenophorea Mesanophrys carcini
867 0.290 Hypotrichia Holosticha heterofoissneri
71 0.174 Euplotia Diophrys oligothrix
1239 0.116 Spirotrichea Protocruzia sp.
Kathablepharidae
Type Percent of eDNA Family Name Type Percent of eDNA Family Name
1361 0.058 Kathablepharidae Katablepharis remigera
Labyrinthulomycetes
Type Percent of eDNA Family Name
160 0.579 Thraustochytriaceae uncultured eukaryote
Malacostraca
Type Percent of eDNA Family Name
1237 0.116 Malacostraca Ianiropsis epilittoralis
Maxillopoda
Type Percent of eDNA Family Name
59 9.412 Misophrioida Misophriopsis okinawensis
Phaeophyceae
Type Percent of eDNA Family Name
146 0.666 Ectocarpales uncultured eukaryote
509 0.637 Ectocarpales Halothrix ambigua
Phytomyxea
Type Percent of eDNA Family Name
210 0.608 Phytomyxea uncultured eukaryote
Polychaeta
Type Percent of eDNA Family Name
447 0.782 Sabellida Rubifabriciola markginsbergi
Rhodymeniophycidae Type Percent of eDNA Family Name
19 9.007 Rhodymeniophycidae Peyssonnelia novae-hollandiae
Syndiniales
Type Percent of eDNA Family Name
1294 0.087 Syndiniales_Group_I uncultured eukaryote
Trichomonadea
Type Percent of eDNA Family Name
180 2.433 Trichomonadea Tetratrichomonas sp.
Ulvophyceae
Type Percent of eDNA Family Name
49 8.109 Ulvophyceae Lithotrichon pulchrum
17 2.751 Ulvophyceae Ulvella viridis
186 1.419 Ulvophyceae Lithotrichon pulchrum
27 1.303 Ulvophyceae Hazenia basiliensis
469 0.724 Ulvophyceae Pseudulvella consociata
716 0.405 Ulvophyceae Phaeophila dendroides
1087 0.174 Ulvophyceae Chaetomorpha sp.
1240 0.116 Ulvophyceae Ruthnielsenia tenuis
uncultured
Type Percent of eDNA Family Name
302 0.145 uncultured uncultured marine
DNA analysis conducted by AquaBiomics LLC.