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Annual Report 2000 - 2002 ANNUAL REPORT 2000 - 2002 Institute of Plant Biochemistry A Leibniz Institute Weinberg 3 06120 Halle (Saale) Germany Phone: +49 (0) 3 45 - 55 82 11 10 Fax: +49 (0) 3 45 - 55 82 11 09 Email: [email protected] www.ipb-halle.de Table of Contents Presentation of the Institute 4 Departmental Organization 7 Department: Stress and Developmental Biology 45 Head: Prof. Dierk Scheel Board of Directors, Foundation Council, Scientific Advisory Board 8 Research Groups: Signal Perception in Plant-Pathogen Interactions 46 Scientific Institute Council, Persons with Special Head: Thorsten Nürnberger Responsibilities, Personnel Committee 9 Cellular Signaling 48 Head: Dierk Scheel Induced Pathogen Defense 50 Department: Natural Product Biotechnology 11 Heads: Sabine Rosahl & Dierk Scheel Head: Prof. Toni M. Kutchan Metal Homeostasis 52 Research Groups: Heads: Dieter Neumann & Stephan Clemens Alkaloid Biosynthesis 12 Publications, Books and Bookchapters, Publications in press, Head: Toni M. Kutchan Patents, Doctoral Theses, Diploma Theses 54 Opium Poppy Biotechnology 14 Head: Susanne Frick Department: Secondary Metabolism 57 Plant Cell Cultures 16 Head: Prof. Dieter Strack Head: Gabriele Herrmann Alkaloid Functional Genomics 18 Research Groups: Head: Jonathan Page Molecular Physiology of Mycorrhiza 58 Mode of Action of Jasmonates 20 Head: Michael H. Walter Heads: Claus Wasternack & Otto Miersch Cell Biology of Mycorrhiza 60 Papaver-Gene Expression Analysis 22 Head: Bettina Hause Head: Jörg Ziegler Biochemistry of Mycorrhiza (since 2002) 62 Publications, Books and Book Chapters, Publications in press, Head: Willibald Schliemann Patents, Doctoral Theses, Diploma Theses 24 Glycosyltransferases 64 Head: Thomas Vogt Biochemistry of Betalains (until 2001) 66 Hops Secondary Metabolism 26 Head: Willibald Schliemann Departments of Natural Product Biotechnology and Bioorganic Chemistry - joint project Heads: Jonathan Page, Jürgen Schmidt, Frederick Stevens (until September 2002) Hydroxycinnamic Acids 68 Head: Dieter Strack Department: Bioorganic Chemistry 29 Publications, Books and Bookchapters, Publications in press, Head: Prof. Ludger Wessjohann Patents, Doctoral Theses, Diploma Theses 70 Research Groups: Department: Synthesis & Method Development 30 Heads: Ludger Wessjohann & Brunhilde Voigt Administration and Technical Services 73 Biocatalysis & Design of Ligands 32 Head: Lothar Franzen Head: Ludger Wessjohann Resources and Investments 74 Plant and Fungal Metabolites / Microanalytics 34 Heads: Norbert Arnold, Jürgen Schmidt, Ludger Wessjohann & Gernot Schneider (until June 2001) Staffing Schedule 75 Structural Analysis & Computational Chemistry 38 Use of Funds from External Sources 76 Heads: Wolfgang Brandt & Andrea Porzel Guest Researchers and Fellows 80 Publications, Books and Bookchapters, Publications in press, Patents, Doctoral Theses, Diploma Theses 40 Press and Public Relations 83 Head: Sylvia Pieplow Searching for Signals: Stress-Induced Changes in Arabidopsis Secondary Metabolite, Peptide and Map & Impressum 86 Protein Patterns (GABI) 43 Departments of Bioorganic Chemistry and Stress and Developmental Biology - joint project Heads: Stephan Clemens, Jürgen Schmidt, Ludger Wessjohann, Dierk Scheel 2 3 Presentation of the Institute he Institute of Plant Bio - of their methodical approaches The large manifold of plant spe- biological function as well as the chemistry (IPB) in Halle was and the equipment at their dispo- cies is reflected in the enormous generation of plants with altered Tfounded on 1 January 1992 sal. This allows interdisciplinary diversity of their natural products. natural product profiles. as a non-university research insti- research using the latest chemical, This content of natural com - tute of the so-called "Blue List". In physiological, cell-biological, bio- pounds is made more complex by Molecular interactions form the 1995, the union of the Blue List chemical, molecular-biological and the change in metabolite patterns basis of cellular function. An Institutes formed the Blue List genetic methods for comprehen- during development as well as interdisciplinary analysis of these Science Association (Wis sen - sive analysis of complex subjects. when a plant is responding to its interactions is therefore of cen- schaftsgemeinschaft Blaue Liste), environment. Knowledge of the tral importance to the research which was subsequently restruc- The IPB is located on the structure and function of natural mission of the Institute of Plant tured and renamed the Leibniz Weinberg Campus, which hosts products is requisite to under- Biochemistry. The optimal adap- Association (Leibniz Ge - the natural science departments standing plant diversity, develop- tation of plants to their habitat meinschaft) in October 1997. The of the Martin Luther University, mental and adaptation processes. depends upon receptor-mediated IPB belongs to the life sciences several non-university institutes New resources can then become perception of biotic and abiotic section of the Leibniz Asso ciation. and biotechnology companies. available for innovative application environmental parameters. Ex ter - The original institute was founded Close relationships and coope- in plant production, plant protec- nal signals are evaluated, compa- as "Arbeits stelle für Bio chemie rations exist between the insti- tion, biotechnology and in the red and converted into physiolo- der Pflanzen" on 1 January 1958 tute, the university and industries. development of biologically active gical responses via altered gene by Prof. Dr. Dr. h.c. mult. Kurt Beside extensive scientific colla- compounds. Furthermore, the rea - expression patterns that are con- Mothes by order of the Ger man boration with several university lization of genome sequencing trolled by cellular and systemic Academy of Science in Berlin. In departments, the institute's de - and the growing availability of ex - signal transduction networks. The 1960 it was renamed Institute for partment heads are full profes- pressed sequence tags of various molecular basis of these proces- Biochemistry of Plants. sors at the university and, there- species is of fundamental impor- ses, receptor/ligand, en zyme/li - fore, involved in teaching and tance to functional genome analy- gand and protein/protein inter- The IPB consists of four scientific supervision of undergraduate and sis. actions, have application in the departments and the administra- graduate students. Together with development of new biologically tion and central services depart- the Institute of Plant Genetics and The comprehensive analysis of active agents. From this perspec- ment. Currently 112 employes Crop Plant Research (IPK) in plant and fungal natural products tive, the mechanisms of communi- work at the IPB paid from the Gatersleben and the Max Planck is a priority in the research mis- cation between plants and their regular budget and another 47 Institutes for Chemical Ecology in sion of the Institute of Plant symbionts and pathogens are in - fun ded by third-party funds. The Jena and of Molecular Plant Biochemistry. Structure analysis, vestigated as are biosynthetic and research profile of the institute is Physiology in Golm the IPB forms synthesis and derivatization of signal transduction pathways. unique within the German scien- the Plant Metabolism Network, natural products contribute to an Chemical struc tures of these in - tific community. The comprehen- PlantMetaNet. This network links understanding of their function teracting components are also sive analysis of natural products the plant metabolomics compe- and to an increase in their struc- modified using gene technological from plants and fungi, the investi- tence that has been developed to tural diversity. This also forms the methods, directed evolution and gation of the interaction of plants an excellent level in these four basis for investigation of their bio- chemical derivatization. The ef - with pathogens, symbionts and plant research institutes in synthesis and for discovering new fects of these changes can be mo - abiotic stresses, studies of mole- Central Germany. biologically active compounds. A nitored in model systems or with cular interactions as part of com- qualitative and quantitative analy- activity screens until a molecule plex biological processes, and sis of natural products in biologi- with the desired characteristics metabolic engineering are at the Research mission statement cal materials requires the deve- (e. g. a drug, a signal compound or center of research activities. Four thematically, methodologi- lopment of suitable analytical an enzyme) is achieved. The deve- Excellent basic research is regar- cally and organisationally overlap- methods. Subsequent identifi- lopment of new syntheses, scree- ded as the indispensable basis for ping research priorities form the cation and isolation of biosynthe- ning tests, assays and analytical the successful implementation of basis of the research mission tic enzymes can provide access to methods is supported by visuali- application-oriented research pro - state ment of the Institute of Plant the encoding genes, which in turn zation of molecular interactions jects. The institute benefits, in par- Biochemistry - plant natural pro- enables study of the regulation of via computer modelling. ticular, from the fact that the ducts, molecular interactions, the biosynthesis. The use of mu - scientific departments of the IPB information technology and meta- tants and transgenic plants ultima- A nexus of natural product re- complement each
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