ChBE Safety Training

July 2016

Emily Edwards Lab Coordinator Chemical & Biological Engineering Tufts University 4 Colby St., Room 151 617-627-2172 Required Training – Chemical Safety Choose 1 of 3 Options A chemical safety training certificate is required before admittance to any

Option 1 Option 2 Option 3 - Preferred • 30-minute video + written test • Classroom training from • Classroom training from • Must get 80% to pass BME Lab Coordinator EH&S, usually at 200 Boston • Certificate expires after one • No set schedule, just when • General information like year demand is high Option 1 • Email me a copy of your • Building-specific information • See website for scheduled certificate – I need proof of • No certificate – send me an times and registration training for the annual lab email so I can add a note to • Email me a copy of your inspections my files certificate Required Training – Bio Safety Classroom Training

Required: Classroom training if working with bio materials Combined: Classroom • Classroom training from EH&S, usually at 200 Boston training for chemical & • General information about bio safety + OSHA Blood- bio materials borne Pathogen Standard • Option 3 above + bio • See website for scheduled times and registration training to the left • Email me a copy of your certificate • See website for scheduled times and registration • Email me a copy of your certificates Personal Protective Equipment (PPE) Tufts EH&S does random unannounced inspections looking for people not wearing PPE

Latex or Nitrile? Check chemical compatibility Write your name Put your name or initials before choosing on your coat on them with a sticker

Face shield and Hearing protection thermal gloves are and sign on door required when are required when dispensing LN using a sonicator (dismembrator) Lab Regulations

• No open-toed shoes allowed in the lab

• Keep a pair of shoes at your desk, if needed

Remove one glove before touching a door knob or anything outside the lab

New Regulation: tubing must be a special cloth-covered steel tubing Lab Regulations

• No backpacks or jackets • No food or drinks including allowed in the lab water

• Request a locker if needed • Leave outside the lab Safety Warning Requirements

The number in Sign on the door each diamond reflects hazards reflects the level inside + contact of danger: 0 through 4 information for after-hours emergencies, must be revised Read the SDS for when needed your chemicals before using Lab Sink – Water Testing

• All lab sinks drain to the Machine Shop • Giant pH neutralizing system and holding tank for periodic testing • Fines for too much metal in the waste water – recent fine for mercury, not sure what else they test for • All chemicals must go into waste containers, never down the sink Labels – No Mystery Substances

Label your containers and samples:

No mystery liquids or solids are allowed in any lab. This includes containers of water and reactor vessels

Use large labels for stock solutions and Use small labels for reactor vessels or small flasks Emergency Procedures

Safety Features inside the lab Calling for help: • Tufts Emergency Number • 617-627-6911 (or 911) • Program this into your phone • 6-6911 from a campus phone

Determining a true emergency: • Fire • Non-minor injuries or burns • Very large chemical spills • Very large gas leaks • Anything involving especially dangerous chemicals or gases • Chemical fume failure if hood contains dangerous chemicals or gases Emergency Procedures

Spill Kit Procedure: Minor Injuries: • Wear PPE • Use 1st Aid Kit • Use absorbent material inside • See Lab Coordinator the bucket to soak up the • Go to Tufts Student liquid Health Services • Place into bag • Fill out waste tag and attach it to the bag • Place the bag in your Pickup After-hours Event: SAA • Fire alarm, chemical smell, etc. • Inform your PI and request a • Call from Tufts Police to the refill from the Lab Coordinator emergency contact number on or on door Chemical Storage Locations

Flammables Cabinet or Special Fridge Inside a • Alcohols • Especially toxic chemicals • Solvents • Disrupts functionality and • Alcohol-fueled safety of hood – must be Bunsen burners minimal • Special flame-proof fridge

Corrosives Cabinet • Separate organic & inorganic acids • Separate acids & bases Biomaterial Storage Locations

Ultra-cold Freezer • -80 °C (-112 °F) • For long-term cell storage • Frost builds up around door and prevents door from closing – must scrape off to maintain temperature

Fridge & Freezer • 4 °C & -20 °C, check storage instructions carefully • No auto-defrosting freezer – they cycle above freezing periodically to melt the frost • Freezers must be defrosted • Must have stickers that say “no food” and “no flammables” Compressed Gas Cylinders

• Clamps mount to table or wall • Chain or strap • Allowed 3 cylinders on 1 clamp

• Top of tank is ceramic and could break if dropped (see Myth Busters episode) • Remove regulator and screw on tightly before moving • Cylinders are safe when cap is on – verified this with our Airgas delivery person Compressed Gas Cylinders

• Types of gases are color-coded: flammable, oxidizer, non- flammable • Each individual gas has a different nozzle thread to avoid mixing of incompatible gases inside the regulator; for

example, CGA 320 is for CO2 • Choose the proper PSI range when purchasing regulator

Flammable Gases have special rules • Allowed only 1500 ft3 (STP) per section of our building, large cylinders have ~285 ft3 = 6 large cylinders. We need more than 6 per section so all flammables must now be “size 80” cylinders. • Tubing must be stainless steel • Flashback arrestor is required: “fuel” for flammable gases and “oxygen” for oxidizers

• Gas sniffers are required for any dangerous gas (H2, H2S, etc.) Chemical Disposal

Do not pour chemicals down the drain – periodically inspected

Liquids – Separate Containers • Acids • Bases + Misc. • Alcohols, solvents

Solids: “Sharps” • Tips New Procedure! • Broken glass Put pipet in sleeve • Empty glass bottles and place in non- sharps bucket Solids: Non-sharp • Powder, “goop” • Chemically-soaked paper towels • Weigh boats • Empty bottles • Pipets in sleeves Chemical Disposal

Tags: Must be on all non-bio containers SAAs – New Procedure! Pickup SAA Immediately fill in: Active SAA • Lab # • PI

As you fill the container, the following must be accurate at all times: • Chemicals + • % (best guess) • Hazards • Leave dates blank until picked up Add new waste here Place full containers here

Keep the Trash the sharps Weekly lab pickup bucket – bin – get new by waste new bag one from Lab management inside Coordinator company Bio-material Disposal Liquids: • Add bleach until v/v % is ~10% • wait at least 30 minutes • pour down drain

Solids: “Sharps” • Tips New Procedure! • Broken glass Put pipet in sleeve • Syringe needles and place in non- • Blades sharps bucket Solids: Non-sharp • Gloves • Bio-soaked paper towels • • Pipets in sleeves Bag must be -able Bio-material Disposal

New Procedure Autoclave Secure lid to bin with tie wraps before leaving your lab • Sterilize glassware, media, lab supplies or non-sharp bio waste • Training required before using • Must sign up for a timeslot before using • Separate training for sterilizing bio waste involving a logbook and tags

Cages for Sharps Bins • Cages must remain locked • Full bins must be logged in logbook • Picked up by Stericyle every 2 weeks Bio vs. Chemical Disposal

What if you have an item that contains Autoclave both bio and chemical material? No chemicals are allowed in the autoclave!

Bio-related chemicals: Other autoclave hazards • Salt • Buffer Non-sharps bio waste • Media in autoclave-able bag

Chemicals that would destroy cells: • Solvents All items must be in • Inorganic autoclave-able tub when compounds inside the autoclave • Alcohols Chemical Fume Hood vs. Bio Cabinet

Fume Hood Bio Cabinet (aka Laminar Flow Hood) • Pulls air in from the room and • Pulls in air from the room, filters and vents it outside to protect person recirculates to prevent bio material from fumes from escaping into the room • Air flow cannot be turned off • Air flow is turned on and off by the user • Sash height and obstructions can • Inside surfaces must be decontaminated greatly affect your safety Fume Hood Safety Proper use of chemical fume hood:

• Hoods are calibrated to be safe for use with the sash below designated height shown on the sticker (usually 18”)

• Keep sash as low as possible without hindering your work – below your shoulders to avoid breathing fumes

• Close the sash when not in use to contain the chemical fumes Facilities Problems

Contact Lab Coordinator: Fill out a Facilities Work Request: • DI water, autoclave, vacuum • Dripping faucet • Low flow in fume hood (containing non- • Electrical problems hazardous chemicals or gas) • Burned out lights • Chemical smell • Restroom problems • Broken equipment • Safety supplies • Any random question Lab Inspections

Annual Lab Inspection: Semi-Annual Chemical Audit: Annual Testing: • General lab safety • Search for especially hazardous • Flowrate of chemical • Fill out form in advance or explosive chemicals fume hoods & bio and fix issues • Also look for storage issues cabinets • Inspected by EH&S • acids & bases not separated • & showers • Receive report with • organic & inorganic acids findings – fix and reply • Receive a report with findings – fix and reply Eco-Friendly Practices Try to avoid disposing of “disposables” in order to reduce our dependence on oil:

• Try to minimize your use of weigh boats, jars & vials • Re-use pipets & tips if using DI water or alcohols • You must dispose of anything that came into contact with live cells Eco-Friendly Practices

• Chemical fume hoods consume a large amount of energy

• Tufts is working hard to reduce the amount of energy consumed in our building

• Close the sash when not in use! Eco-Friendly Practices

• Paper, rigid plastic, glass & metal are banned from landfills in MA

• See the signs at the recycling stations for more information Eco-Friendly Practices

Which is more environmentally friendly? Paper towels vs. Kimwipes

Must consider the energy used for manufacturing and transportation Department Website

This presentation will be available on the ChBE website under the Graduate Program tab