RNF8 Antibody Cat. No.: 5763

RNF8 Antibody

Immunofluorescence of RNF8 in rat lung tissue with RNF8 antibody at 20 μg/mL.

Specifications

HOST SPECIES: Rabbit

SPECIES REACTIVITY: Human, Mouse, Rat

HOMOLOGY: Predicted species reactivity based on immunogen sequence: Bovine: (92%)

RNF8 antibody was raised against a 14 amino acid synthetic peptide near the carboxy terminus of human RNF8. IMMUNOGEN:

The immunogen is located within amino acids 330 - 380 of RNF8.

TESTED APPLICATIONS: ELISA, IF, WB

September 26, 2021 1 https://www.prosci-inc.com/rnf8-antibody-5763.html RNF8 antibody can be used for detection of RNF8 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunoflourescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL. APPLICATIONS:

Antibody validated: Western Blot in human samples and Immunofluorescence in rat samples. All other applications and species not yet tested.

At least three isoforms of RNF8 are known to exist; this antibody will detect all three SPECIFICITY: isoforms.

POSITIVE CONTROL: 1) Cat. No. 1302 - Human Lung Tissue Lysate

Predicted: 46, 53 kDa PREDICTED MOLECULAR WEIGHT: Observed: 50 kDa

Properties

PURIFICATION: RNF8 Antibody is affinity chromatography purified via peptide column.

CLONALITY: Polyclonal

ISOTYPE: IgG

CONJUGATE: Unconjugated

PHYSICAL STATE: Liquid

BUFFER: RNF8 Antibody is supplied in PBS containing 0.02% sodium azide.

CONCENTRATION: 1 mg/mL

RNF8 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one STORAGE CONDITIONS: year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Additional Info

OFFICIAL SYMBOL: RNF8

RNF8 Antibody: hRNF8, KIAA0646, E3 -protein ligase RNF8, RING finger protein 8, ALTERNATE NAMES: hRNF8

ACCESSION NO.: NP_003949

PROTEIN GI NO.: 4504867

GENE ID: 9025

USER NOTE: Optimal dilutions for each application to be determined by the researcher.

Background and References

September 26, 2021 2 https://www.prosci-inc.com/rnf8-antibody-5763.html RNF8 Antibody: RNF8 was identified as a (E3) containing a RING finger motif and a FHA domain. This protein has been shown to interact with several class II ubiquitin-conjugating including UBE2E1/UBCH6, UBE2E2, and UBE2E3. RNF8 assembles at DNA double-strand breaks (DSBs) via interactions though the FHA domain BACKGROUND: with the adaptor protein MDC1, resulting in an increase in DSB-associated H2A ubiquitinations mediated by the associated ubiquitin ligase RNF168 followed by the accumulation of 53BP1 and BRCA1 repair proteins. Together with RNF168, RNF8 plays an integral part of class switch recombination in B cells, allowing the production of several classes of antibodies, through the recruitment of 53BP1 and BRCA1 to the DSB sites.

1) Ito K, Adachi S, Iwakami R, et al. N-Terminally extended human ubiquitin-conjugating REFERENCES: enzymes (E2s) mediate the ubiquitination of RING-finger proteins, ARA54 and RNF8. Eur. J. Biochem. 2001; 268:2725-32.

2) Mailand N, Bekker-Jensen S, Faustrup H, et al. RNF8 ubiquitylates at DNA double-strand breaks and promotes assembly of repair proteins. Cell 2007; 131:887-900.

3) Doil C, Mailand N, Bekker-Jensen S, et al. RNF168 binds and amplifies ubiquitin conjugates on damaged to allow accumulation of repair proteins. Cell 2009; 136:435-46.

4) Ramachandran S, Chahwen R, Nepal RN, et al. The RNF8/RNF168 ubiquitin ligase cascade facilitates class switch recombination. Proc. Natl. Acad. Sci. USA 2010; 107:809-14.

ANTIBODIES FOR RESEARCH USE ONLY.

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