Analyte Quarterly Vol. 2 2018 Your comprehensive guide to multiplex and single protein detection

• MILLIPLEX® map Assays • SMC™ (Single Molecule Counting) Assays • ELISA • RIA • Custom Assay Development

The life science business of Merck operates as MilliporeSigma in the U.S. and Canada. Look Closer All biomarker kits are not created equal

Your time and samples are too precious to trust to anything but the most highly validated kits available. With assay performance data included in every protocol, you can discover with confidence.

2 | MerckMillipore.com/immunoassays Reproducible Results Rigorous Assay Development you can Trust

Specificity Stability • Antibody specificity is tested to ensure • All kits are rigorously tested for shipping negligible cross-reactivity of each antibody stability to determine impact on each analyte pair with other analytes and proteins in the • Sample stability is examined at a range of validated sample types temperatures, and by freeze/thaw; if samples • Assay specificity is ensured by confirming require specific conditions, it is noted in the consistent performance of every analyte in protocol both singleplex and multiplex formats Precision and Accuracy Method Comparisons • Intra-assay and inter-assay precision levels • We test our kits against in-house kits with the are typically within 10-15% same analytes, and against kits from other • Accuracy is normally within ± 20% vendors when available, ensuring we launch the highest performing kits Linearity of Dilution Selectivity • Spike recovery in sample is performed for each analyte at three concentrations on the • Assay buffers and bead diluents are optimized standard curve; diluted sample results must to enhance antibody binding to only those be directly proportional to the concentration of analytes of interest in the presence of analyte in the sample, typically within ± 20% sample matrix

Lot-to-Lot Consistency Detection and Sensitivity • A “gold standard” calibrator is used as a • The detection range and reference for all future production lots minimum detectible concentrations (minDCs or LLOQ) for each analyte is Dedicated Support published in our protocols, • Committed care when and where you need it, and is based on actual from our sales specialists, technical support samples scientists, customer support, and custom immunoassay development team Cross-Talk

• Proper maintenance We’ll help you choose the right platform of instrumentation eliminates or minimizes and assays to meet your needs today, cross-talk between and we’ll partner with you to meet those analytes in the assay needs in the future—without compromising quality. Performance in a Sample Matrix • Assays are validated for the specific sample type, and for kits validated for serum and plasma, we always provide an optimized native serum matrix to mimic the environment of the sample, normalizing assay performance

MerckMillipore.com/immunoassays | 3 FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. PROTEIN DETECTION PLATFORMS 4 |MerckMillipore.com/immunoassays MILLIPLEX platforms. Whetheryou fromamultiplexpanelusingLuminex needrichprofilingdata study requirements? Having offering a scientificpartner assay youa ofhighperformance variety What’s thanawell-validated, better sensitive, reproducible, robustimmunoassay thatfits your platform foryour needs We’re here toguideyou tochoosethebestproteindetection Platforms FitforYour Purpose Flexibility and sensitivity: ourplatformsfit your purpose andsensitivity: Flexibility • ELISAs • • SMC™ Assays forSMCxPRO™ orErenna • • • GoodPerformance ultrasensitive detectionfromanSMC™assay, orrobustELISAswehelpyou you getdata trust. ELISA SMCxPRO™ or Erenna Platforms Detection Protein Luminex >1,800 ELISAs, EIAsandRIAsagainstnumerous solublecirculatingandintracellularbiomarkers Measure tothefemtogram/mL level Previously assays, undetectable nowquantified fromwhichtochoose Broadest selectionofanalytes Widest rangeofresearch areas—and species ® platform ® Fit forPurpose High performance High performance Ultrasensitivity Flexible platform Multiplex detection Most widelycited Plate reader compatibility mapMultiplexDetection •• StrongPerformance ® system Quantitative ••• SuperiorPerformance Yes Yes Yes Sensitivity pg/mL pg/mL fg/mL ® Instruments 50–100 µL 5–100 µL ≤25µL Volume Sample NotRecommended Dynamic Range ••• ••• •• Capability Multiplex ® technology, Recommended Support Custom Assay Assay Yes Yes Yes

PROTEIN DETECTION PLATFORMS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.

NEW KITS 40-301 40-300 40-006 40-007 Cat.No. Cat. No. 03-0145-00 03-0160-00 03-0161-00 03-0156-00 03-0159-00 03-0164-00 03-0157-00 03-0163-00 Coming Soon! Coming Soon! Coming Soon! Coming Soon! Coming Soon! Coming Soon! Coming Soon! Coming Soon! HCABMAG-13K HIL18MAG-66K HAGE1MAG-20K MerckMillipore.com/immunoassays | 5 MerckMillipore.com/immunoassays instruments ® Human Human Human Human, Mouse, Rat Human, Mouse, Human Human Human Human Human Human Human N/A N/A N/A N/A Species Bovine Species Canine Human Human Human Human Human N/A and Single Protein Detection Kits Protein map and Single ® map Panels map Panels ® ® Lyophilization of the standard provides the kit at one storage condition the of the standard provides Lyophilization step of a plate transfer Removal to accommodate automation volumes reagent Increased and Erenna on both SMCxPRO™ to work verified Kits are Improved options for sample prep and incubation options for sample prep Improved Cytokine Panel Cytokine Aging Panel 1 Aging Panel Cancer Autoantibody Singleplex IL-18 SMC™ Streamlined KitsSMC™ Streamlined Beta Kit 1-40 High SensitivityAmyloid Immunoassay Kit High Sensitivity Immunoassay IL-1β Kit High Sensitivity Immunoassay IL-4 Kit High Sensitivity Immunoassay IL-13 Kit High Sensitivity Immunoassay IL-17A Kit High Sensitivity Immunoassay IL-17F Kit High Sensitivity Immunoassay IL-23 Kit High Sensitivity Immunoassay TNFα Instrumentation BioTek 50 TS Plate Washer, BioTek 800 TS Absorbance Reader-Basic, 5 Software 800 TS Absorbance Reader-Gen 5 Secure 800 TS Absorbance Reader-Gen Description Description MILLIPLEX Pituitary Panel MILLIPLEX SMC™ Streamlined KitsSMC™ Streamlined Kit High SensitivityBAFF Immunoassay MIP-1β/CCL4 Plate-based Immunoassay Kit Plate-based Immunoassay MIP-1β/CCL4 IL-1α High Sensitivity Immunoassay Kit High Sensitivity Immunoassay IL-1α IL-5 High Sensitivity Immunoassay Kit High Sensitivity Immunoassay IL-5 IL-22 High Sensitivity Immunoassay Kit High Sensitivity Immunoassay IL-22 Plate Washer Verification Kit Verification Plate Washer • • • • • New SMC™ Streamlined Kit Improvements: Kit Improvements: New SMC™ Streamlined work to do your Making it easier for you Coming Soon NEW! MILLIPLEX NEW IMMUNOASSAY PRODUCTS IMMUNOASSAY NEW FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. MILLIPLEX® map KIT INDEX

MILLIPLEX® map 48-690MAG...... 48-683MAG...... 48-682MAG...... 48-681MAG...... 48-680MAG...... 48-669MAG...... 48-660MAG...... 48-655MAG...... 48-650MAG...... 48-633MAG...... 48-632MAG...... 48-631MAG...... 48-630MAG...... 48-628MAG...... 48-626MAG...... 48-625MAG...... 48-624MAG...... 48-623MAG...... 48-622MAG...... 48-621MAG...... 48-619MAG...... 48-618MAG...... 48-614MAG...... 48-612MAG...... 48-611MAG...... 48-610MAG...... 48-602MAG...... 46-713MAG...... 46-694MAG...... 46-686MAG...... 46-685MAG...... 46-677MAG...... 46-675MAG...... 46-667MAG...... 46-656MAG...... 46-655MAG...... 46-654MAG...... 46-618MAG...... 46-613MAG...... 46-612MAG...... 46-610MAG...... 46-609MAG...... 46-604MAG...... 46-602MAG...... 4 MILLIPLEX CPTMAG-96K...... CKT2MAG-97K...... CKT1MAG-97K...... CGTMAG-98K...... CCYTOMAG-90K...... CCYTMG-90K-PX13...... C APOMAG-62K...... A 6 |EMDMILLIPORE.COM/MILLIPLEXMerckMillipore.com/milliplex Cat. No.Cat. ® mapKitIndex Page 17 16 16 16 16 16 16 17 17 17 17 17 17 18 18 18 18 18 18 16 18 17 17 15 15 17 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 19 14 14 14 14 14 14 9 HKI6MAG-99K...... HKI5MAG-99K...... HKI4MAG-99K...... HKI3MAG-99K...... HKI2MAG-99K...... HKI1MAG-99K...... HIL18MAG-66K...... HIGFMAG-52K...... HIGFBMAG-53K...... HGAMMAG-303E...... HGAMMAG-301K...... HDIAB-34K-PMX5...... HCYTOMAG-60K...... HCYTMAG-60K-PX41...... HCYTMAG-60K-PX38...... HCYTMAG-60K-PX30...... HCYTMAG-60K-PX29...... HCYP4MAG-64K HCYP3MAG-63K HCYP2MAG-62K HCY4MG-64K-PX21...... HCVD6MAG-67K...... HCVD5MAG-67K...... HCVD4MAG-67K...... HCVD3MAG-67K...... HCVD2MAG-67K...... HCVD1MAG-67K...... HCP2MAG-62K-PX23...... HCMP2MAG-19K...... HCMP1MAG-19K...... HCMBMAG-22K...... HCKPMAG-11K...... HCD8MAG15K17PMX...... HCD8MAG-15K...... HCCBP3MAG-58K...... HCCBP2MAG-58K...... HCCBP1MAG-58K...... HCCB4MAG-58K...... HCABMAG-13K...... HBNMAG-51K...... HANG2MAG-12K...... HAIAB-10K...... HAGP1MAG-12K...... HAGE1MAG-20K...... HADK2MAG-61K...... HADK1MAG-61K...... HADCYMAG-61K...... H FMHMAG-29K...... FCYTMAG-20K-PMX...... F EQCYTMG-93KPX23...... EQCYTMAG-93K...... E Cat. No.Cat...... Page 10 10 10 10 10 10 10 10 10 10 10 10 14 14 14 14 7 9 9 8 8 8 7 7 7 7 7 7 7 7 7 9 9 9 9 9 9 7 8 8 9 7 7 9 9 9 8 9 8 8 8 Human pp. 7-10; Primatep. 11;Mousepp. 11-13; Ratpp. 13-14; HSTC384-28K...... HSP3MAG-63K...... HSP2MAG-63K...... HSP1MAG-63K...... HSCRMAG32KPX14...... HSCRMAG-32K...... HRNKLMAG-51K-01...... HPTP1MAG-66K...... HPRTKMAG-01K...... HNS2MAG-95K...... HNS1MAG-95K...... HNPMAG-35K...... HNDG4MAG-36K...... HNDG3MAG-36K...... HNDG2MAG-36K...... HNDG1MAG-36K...... HND3MAG-39K...... HNCSMAG-35K...... HNABTMAG-68K...... HMYOMAG-56K...... HMMP2MAG-55K HMMP1MAG-55K HMHEMAG-34K...... HLPPMAG-57K...... HLINJMAG-75K...... MKI1MAG-94K...... MHSTCMAG-70KPMX...... MHSTCMAG-70K...... MGAMMAG-300K...... MGAMMAG-300E...... MECY2MAG-73KPX...... MECY2MAG-73K...... MCYTOMAG-70K-PMX...... MCYTOMAG-70K...... MCYTMAG-70K-PX32...... MCVD2MAG-77K...... MCVD1MAG-77K...... MCD8MAG48K-PX15...... MCD8MAG-48K MBNMAG-41K...... MAP2MAG-76K...... MAGPMAG-24K...... MADPNMAG-70K-01...... MADKMAG-71K...... MADCYMAG-72K...... MABMAG-83K M HTMP2MAG-54K...... HTMP1MAG-54K...... HTH17MAG-14K...... HT17MG-14K-PX25...... HSTCMAG384-PX21...... HSTCMAG28SPMX21...... HSTCMAG28SPMX13...... HSTCMAG-28SK...... Cat. No.Cat...... Other Species p. 14;CellSignaling pp. 15-19. Page 16 10 10 10 10 10 10 10 10 10 10 10 13 11 11 12 12 11 11 11 11 11 12 12 11 11 12 12 12 12 12 12 13 8 8 8 8 8 9 9 8 8 8 8 9 8 8 7 7 7 7 7 7 7 MTH17MAG-47K...... MT17MAG47K-PX25...... MSHMAG-21K...... MSCRMAG-42K...... MPTMAG-49K...... MMYOMAG-74K...... MMMP3MAG-79K...... MMMP2MAG-79K...... MMMP1MAG-79K...... MMHMAG-44K...... MKI2MAG-94K...... PRCYTOMAG-40K...... PRCYTMAG40K-PX25...... PRCYTMAG40K-PX24...... PRCYT2MAG40K...... PCYTMG-40K-PX23...... PCYTMG-23K-13PX...... PCYTMAG-23K...... P NHPPT1MG-46K...... NHPMHMAG-45K...... N TGFBMAG-64K-03...... TGFBMAG-64K-01...... T SKINMAG-50K...... S RV3MAG-26K...... RV2MAG-26K...... RV1MAG-26K...... RTHYMAG-30K...... RSHMAG-69K...... RPTMAG-86K...... RMYOMAG-88K...... RMNPMAG-83K...... RMHMAG-84K...... RLI1MAG-92K...... RKTX2MAG-37K...... RKTX1MAG-37K...... RECYTMAG-65K...... RECYMAG65K27PMX...... RCI1MAG-87K...... RBN1MAG-31K...... RADPKMAG-80K...... RADPCMAG-82K...... R Cat. No.Cat. 12, 13,14 12, 13,14 9, 11,12, 13, 14 Page 8, 11, 8, 11, 11 11 12 12 12 12 12 12 12 13 11 11 11 11 11 14 14 11 11 14 13 13 13 13 13 13 13 13 14 14 14 13 13 13 14 13 13 8

MILLIPLEX® map IMMUNOLOGY FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.

HUMAN (mature) (LTA) IL-12 (p70) IL-12 IL-13 / CTLA8 IL-17A IL-21 IL-23 / CXCL11 I-TAC / CCL3 MIP-1α / CCL4 MIP-1β / CCL20 MIP-3α TNFα IL-4 IL-5 IL-6 IL-10 IL-13 / CCL3 MIP-1α / CCL4 MIP-1β Perforin TNFα / IL-25 IL-17E IL-17F IL-21 IL-22 IL-23 IL-27 / IFNλ2 IL-28A IL-31 / NF-HEV IL-33 / CCL20 MIP-3α TNFα TNFβ/Lymphotoxin-α ) HT17MG-14K-PX25 ) HSTCMAG384-PX21 MerckMillipore.com/milliplex | 7 MerckMillipore.com/milliplex ) HCD8MAG-15K (Cat. No. ) HSTCMAG384PX21BK (Bulk Cat. No. (Cat. No. HCD8MAG15K17PMX) (Cat. No. HCD8MAG15KPX17BK) (Bulk Cat. No. (Bulk Cat. No. HT17MAG14PMX25BK) (Bulk Cat. No. (Cat No. (Cat. No.

TNFRSF9 Serum/Plasma only samples only Tissue Culture format assay Competitive Available for custom premix Available sample a different Analyte requires dilution from others in panel

/ CX3CL1 Fractalkine GM-CSF IFNγ IL-1β IL-2 IL-4 IL-5 IL-6 IL-7 / CXCL8 IL-8 IL-10 sCD137/4-1BB/ / TNFRSF6 sFAS sFasL GM-CSF Granzyme A Granzyme B IFNγ IL-2 GM-CSF IFNγ IL-1β IL-2 IL-4 IL-5 IL-6 IL-9 IL-10 (p70) IL-12 IL-13 IL-15 / CTLA8 IL-17A EMDMILLIPORE.COM/MILLIPLEX | 7 EMDMILLIPORE.COM/MILLIPLEX 21 21 17 17 25 25 384-well Human High High Human 384-well Sensitivity T Cell ) HSTC384-28K (Cat.No. Human Th17 HTH17MAG-14K) (Cat. No. Human CD8+ T Cell

● ★ ^ ◦ ♦ ♦ ♦ ♦ ♦ NEW IL-12 (p70) IL-12 IL-13 / CTLA8 IL-17A IL-21 IL-23 / CXCL11 I-TAC / CCL3 MIP-1α / CCL4 MIP-1β / CCL20 MIP-3α TNFα M-CSF MIG / CXCL9 / CCL20 MIP-3α / CCL19 MIP-3β ▲ / CXCL7 NAP-2 / IFNλ3 IL-28B IL-32α IL-34 IL-35 / IL-1F8 IL-36β / IL-1F7 IL-37 / IL-1F10 IL-38 / CCL18 / PARC MIP-4 MPIF / CCL23 YKL40/CHI3L1 ) HSTCMAG28SPMX13 ) HCY4MG-64K-PX21 ) HCP4MG64KPX21BK ♦ ♦ ♦ ♦ ♦ ♦ ♦ ♦ ♦ These analytes cannot be plexed withThese analytes cannot be plexed other analytes in this panel in serum/ plasma a protease inhibitor during Requires sample collection sample extraction Requires No standard/QC; autoantibody levels as MFIs reported are ) HSTCMAG-28SK (Cat. No. ) HCYP4MAG-64K (Cat. No. ) HSTCMAG28PMX13BK (Bulk Cat. No. (Cat. No. ) HSTCMAG28SPMX21 (Cat. No. ) HSTCMAG28PMX21BK (Bulk Cat. No. (Cat. No. (Cat. No.

/ CX3CL1 Fractalkine GM-CSF IFNγ IL-1β IL-2 IL-4 IL-5 IL-6 IL-7 ♦ / CXCL8 IL-8 IL-10 IL-18 APRIL / TNFSF13 / Blys BAFF BRAK / CXCL14 CCL28 CXCL16 / CCL16 HCC-4 HMGB1 ● IFNβ / α-Taxilin IL-14 IL-19 IL-24 / CCL14 ▲ HCC-1 IL-11 / IFNλ1 IL-29 / CXCL11 I-TAC / GCP-2 LIX / CXCL6 / XCL1 Lymphotactin ▲  ◗ ■ ** 21 13 13 21 21 21 Human IL-18 Singleplex Human IL-18 ) HIL18MAG-66K (Cat. No. Human High Sensitivity T Cell Human Cytokine/Chemokine IV Panel Human Cytokine/ChemokineHuman III Panel ) HCYP3MAG-63K (Cat.No. map kits ♦ ® ▲ ▲ ♦ ♦ ♦ ▲ ♦ ♦ ♦ ♦ ♦ ♦ (mature) (LTA) IL-28A/IFNλ2 IL-33/NF-HEV LIF MCP-2/CCL8 MCP-4/CCL13 MIP-1δ/MIP-5/CCL15 SCF SDF-1/CXCL12 TARC/CCL17 TPO TRAIL/TNFSF10 TSLP IL-9 IL-10 (p40) IL-12 (p70) IL-12 IL-13 IL-15 / CTLA8 ♦ IL-17A ♦ / CXCL10 IP-10 ♦ / CCL2 MCP-1 / CCL7 MCP-3 MDC / CCL22 ♦ / CCL3 MIP-1α ♦ / CCL4 MIP-1β PDGF-AA BB / PDGF-AB RANTES / CCL5 ♦ TGFα TNFα TNFβ / Lymphotoxin-α VEGF-A

) HCP2MAG-62K-PX23 ) HCYTMAG-60K-PX29 ♦ ♦ Analytes which cannot be plexed together: and total – Active and total – Free listed in Cat. No. Available Premix panel only Premix ♦ ♦ ♦ ♦ ♦ ♦ ♦ ♦ ♦ ♦ ♦ ♦

♦ ▼ Legend key for MILLIPLEX key Legend † Bulk indicates Space Saver Packaging Bulk indicates Space Saver ) HCYP2MAG-62K (Cat. No. ) HCYTOMAG-60K (Cat.No. ) HCP2MAG62KPX23BK (Bulk Cat. No. ) HCYTMAG60PMX29BK (Bulk Cat. No.

(Cat. No. ) HCYTMAG60PMX41BK (Bulk Cat. No. ) HCYTMAG60PMX30BK (Bulk Cat. No. ) HCYTMAG-60K-PX38 (Cat. No. ) HCYTMAG60PMX38BK (Bulk Cat. No. ) HCYTMAG-60K-PX41 (Cat. No. (Cat. No. ) HCYTMAG-60K-PX30 (Cat. No. MPIF-2 Exodus-2

BCA-1/CXCL13 CTACK/CCL27 ENA-78/CXCL5 Eotaxin-2/CCL24/ Eotaxin-3/CCL26 I-309/CCL1 IL-16 IL-20 IL-21 IL-23 6Ckine/CCL21/ sCD40L EGF Eotaxin / CCL11 ♦ / FGF-basic FGF-2 Flt3 Ligand / CX3CL1 Fractalkine G-CSF GM-CSF GRO IFNα2 IFNγ IL-1α IL-1β IL-1Ra IL-2 IL-3 IL-4 IL-5 IL-6 IL-7 ♦ / CXCL8 IL-8 23 23 41 30 38 38 41 29 29 30 Human Cytokine/Chemokine II Panel Immunology Human Cytokine/Chemokine I Panel HUMAN FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. MILLIPLEX® map IMMUNOLOGY / IMMUNE RESPONSE / METABOLISM / ENDOCRINOLOGY

HUMAN (Cat. No.(Cat. HCMP1MAG-19K) PanelHuman Complement 1 No.(Cat. HSCRMAG-32K) Receptor Human SolubleCytokine (Bulk Cat. No.(Bulk Cat. TGFBMAG-64K-01BK) No.(Cat. TGFBMAG-64K-01) Singleplex Multi-Species TGFβ – No.(Cat. HTMP2MAG-54K) culture(Cell samples) Human TIMPPanel 2 No.(Cat. HTMP1MAG-54K) (Serum/Plasma samples) Human TIMPPanel 1 No.(Cat. HMMP2MAG-55K) Human MMPPanel 2 No.(Cat. HMMP1MAG-55K) Human MMPPanel 1 No.(Cat. HCMP2MAG-19K) PanelHuman Complement 2 8 |EMDMILLIPORE.COM/MILLIPLEXMerckMillipore.com/milliplex 14 14 TGFβ1 TIMP-2 TIMP-1 TIMP-1 MMP-7 MMP-2 MMP-1 MMP-12 MMP-3 C4 C3b /iC3b C3 C1q C5 C4b C2 Adipsin/Factor D sIL-4R sIL-2Rα sIL-1RII sIL-1RI sGP130 sEGFR/sHER1/ErbB1 sCD30 No. (Cat. (Bulk Cat. No.(Bulk Cat. HSCRMAG32PMX14BK) HSCRMAG32KPX14) TIMP-4 TIMP-3 TIMP-2 MMP-10 MMP-9 MMP-13 Factor P/Properdin Factor H Factor B Mannose-binding Factor I C9 C5a sVEGFR3 /sFlt-4 sVEGFR2 /sKDRsFlk-1 sVEGFR1 /sFlt-1 sTNF RII sTNF RI sRAGE sIL-6R Lectin (MBL) Isotyping Isotyping Human Immunoglobulin No.(Cat. SKINMAG-50K) only) (Skin extracts Human Skin (Cat. No.(Cat. HSP3MAG-63K) Human SepsisPanel 3 No.(Cat. HSP2MAG-63K) Human SepsisPanel 2 (Cat. No.(Cat. HAIAB-10K) (Cat. No.(Cat. HSP1MAG-63K) Human SepsisPanel 1 Autoantibody** Human Autoimmune Immune Response No.(Cat. TGFBMAG-64K-03) Multi-Species TGFβ –3Plex Proteinase 3 PM/Sci 100 PL-12 (Alanyl-tRNA PCNA (Proliferating Myeloperoxidase Mi-2 Ku Jo-1 CENP-B (Centromere CENP-A (Centromere C1q β2-Glycoprotein NON-CONFIGURABLE KIT IgG2 IgG1 IgA Human Serum Fibronectin ^ Cortisol NGAL/Lipocalin-2 Neutrophil Elastase-2 Lactotransferrin (LTF) IL-8 /CXCL8 IL-1α HSP70 Granzyme B sICAM-1 sFasL sFAS /TNFRSF6 TGFβ2 TGFβ1 Synthetase) Antigen A) Cell Nuclear (MPO) Protein B) Protein A)

Albumin (HSA) (ELA2) (Cat. No.(Cat. HGAMMAG-301K) SB/La (Sjögren’s SSA/Ro60 SSA/Ro52 (Sjögren’s Sm Scl-70 RNP/Smith (RNP/ RNP Ribosomal P IgM IgG4 IgG3 Keratin-6 Keratin-1,10 Involucrin Thrombospondin-1 Resistin MMP-8 MIP-1β /CCL4 MIP-1α /CCL3 sVCAM-1 PAI-1 (total) MIF TGFβ3 antigen B/La) Syndrome-related kDa) antigen A/Ro60 Syndrome-related (Sjögren’s kDa) antigen A/Ro52 Syndrome-related Sm) (Ribonucleoprotein) (TSP-1) ▼ Human Adipocyte Human Adipocyte No.(Cat. HADK2MAG-61K) (Serum/Plasma samples) Human AdipokinePanel 2 No.(Cat. HADK1MAG-61K) (Serum/Plasma samples) Human AdipokinePanel 1 Endocrinology Metabolism/ No.(Cat. HGAMMAG-303E) Human IgE –Singleplex (Cat. No.(Cat. HMYOMAG-56K) Human Myokine No.(Cat. HMHEMAG-34K) Hormone Human Metabolic Human Diabetes No.(Cat. HADCYMAG-61K) culture(Cell samples) 5 5 NON-CONFIGURABLE KIT Insulin IL-8/CXCL8 IL-6 IL-1β HGF NGAL/Lipocalin-2 Adipsin/Factor D Adiponectin IgE IL-6 FSTL1 Fractalkine/CX3CL1 FGF-21 FABP3 (EPO) Erythropoietin BDNF Apelin Glucagon †◗ (total) GLP-1 (active) †◗ GLP-1 GIP (total) Ghrelin (active) C-Peptide †◗ (total) Amylin (active) †◗ Amylin Leptin IL-8/CXCL8 IL-6 IL-1β HGF Adiponectin Glucagon GLP-1 (active) C-Peptide (Cat.No. (Bulk Cat. No.(Bulk Cat. HDIAB34KPMX5BK) ◗ ◗ HDIAB-34K-PMX5) ◗ ◗ TNFα NGF MCP-1/CCL2 Leptin Resistin PAI-1 (total) Osteonectin/SPARC Osteocrin (OSTN)/ (OSM) Oncostatin-M Myostatin/GDF8 LIF Irisin IL-15 TNFα PYY (total) Pancreatic MCP-1/CCL2 Leptin Insulin IL-6 TNFα Resistin PAI-1 (total) NGF MCP-1/CCL2 Leptin Insulin ▼ Musclin Polypeptide (PP) MILLIPLEX® map METABOLISM / ENDOCRINOLOGY / CVD / BONE / CANCER FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.

HUMAN NEW Osteocalcin (OC) Osteopontin (OPN) (OPG) Osteoprotegerin PTH Sclerostin (SOST) TNFα GITRL HVEM ICOS LAG-3 PD-1 PD-L1 TIM-3 TLR-2 IGF2BP2/IMP2 IGF2BP3/IMP3 Mucin-1 (MUC1) P16-INK4a p53 SOX2 Survivin/BIRC5 IL-8/CXCL8 Leptin MIF Osteopontin (OPN) Prolactin PSA (free)† PSA (total)† SCF TGFα TNFα TRAIL/TNFSF10 VEGF-A MerckMillipore.com/milliplex | 9 MerckMillipore.com/milliplex Serum/Plasma only samples only Tissue Culture format assay Competitive Available for custom premix Available sample a different Analyte requires dilution from others in panel ACTH DKK1 FGF-23 IL-1β IL-6 Insulin Leptin RANKL BTLA CD27 CD28 CD40 CD80/B7-1 CD86/B7-2 CTLA-4/CD152 GITR CCNB1/Cyclin B1 CCNB1/Cyclin CTAG1B/NY-ESO-1 ENO1 Galectin-1 (GAL1) Galectin-3 (GAL3) HER2/ErbB2 HIF-1α HSP60 (AFP) α-Fetoprotein CA125 CA15-3 CA19-9 CEA CYFRA21-1 sFAS/TNFRSF6 sFasL FGF-2/FGF-basic HCGβ HE4 HGF IL-6 EMDMILLIPORE.COM/MILLIPLEX | 9 EMDMILLIPORE.COM/MILLIPLEX Bone Human Bone ) HBNMAG-51K (Cat.No. Singleplex Human RANKL – ) HRNKLMAG-51K-01 (Cat. No. Cancer Human Immuno-Oncology Checkpoint Protein ) HCKPMAG-11K (Cat. No. Human Cancer Autoantibody** ) HCABMAG-13K (Cat. No. Human Circulating Cancer 1 Panel Biomarker ) HCCBP1MAG-58K (Cat. No.

● ★ ^ ◦ (LEPR)/OB-R (PF4)/CXCL4 (SAP) (vWF) LRG1 MCAM/MUC18 ucMGP Apo CII Apo CIII Apo E Pentraxin-3 (PTX3) Pentraxin-3 Thrombomodulin (TF) Tissue Factor T (TnT) Troponin Leptin Receptor MR-ProADM Notch1 PLA2G7 PTGDS sST2/IL1RL1 Syndecan 4 (SYND4) Myoglobin NGAL/Lipocalin-2 sP-Selectin A Serum Amyloid sVCAM-1 Haptoglobin sL-Selectin 4 Platelet Factor P Serum Amyloid Willebrand Factor von These analytes cannot be plexed withThese analytes cannot be plexed other analytes in this panel in serum/ plasma a protease inhibitor during Requires sample collection sample extraction Requires No standard/QC; autoantibody levels as MFIs reported are (CHGA/CGA) (AGP) (MPO) Apo AI Apo AII Apo B sCD14 DPP4 Endostatin pGRN sCD31/sPECAM-1 dPAPP-A sE-Selectin (FST) Follistatin ADAM15 Cadherin 13 (CDH13) sCD163 Chromogranin-A HSP60 IGF1R LDLR α-1-Acid Glycoprotein Glycoprotein α-1-Acid α-2-Macroglobulin D Adipsin/Factor CRP A Fetuin Fibrinogen ADAMTS13 D-dimer GDF-15 sICAM-1 Myeloperoxidase ▲  ◗ ■ ** Human CVD Panel 6 Human CVD Panel ) HCVD6MAG-67K (Cat. No. Human Apolipoprotein ) APOMAG-62K (Cat. No. Human CVD Panel 4 Human CVD Panel HCVD4MAG-67K) (Cat. No. 5 Human CVD Panel ) HCVD5MAG-67K (Cat. No. Human CVD Panel 3 Human CVD Panel (Acute Phase) HCVD3MAG-67K) (Cat. No. Human CVD Panel 2 CVDHuman Panel HCVD2MAG-67K) (Cat.No. map kits ◗ ® ^

■ NEW ★ Factor (PLGF) Factor LIX/CXCL6/GCP-2 NT proBNP Oncostatin-M (OSM) Placental Growth I (TnI) Troponin FSH GH LH TSH Testosterone T3 T4 IGF-2 IGFBP5 IGFBP6 IGFBP7 FGF-19 FGF-21 FGF-23 HGF IL-18 Jag1 Leptin Wnt-3a IL-10 ■

Analytes which cannot be plexed together: and total – Active and total – Free listed in Cat. No. Available Premix panel only Premix

♦ ▼ Legend key for MILLIPLEX key Legend † Bulk indicates Space Saver Packaging Bulk indicates Space Saver Protein (AgRP) BNP CK-MB CXCL16 Endocan-1 (ESM-1) FABP3 FABP4 LIGHT Cortisol Estradiol Progesterone CNTF ACTH Agouti-Related IGF-1 IGFBP1 IGFBP2 IGFBP3 IGFBP4 α-Fetoprotein (AFP) α-Fetoprotein ANGPTL3 ANGPTL4 ANGPTL6 FABP1 CTACK/CCL27 FGF-21 GDF-11 GDF-15 GnRH IL-6 Human CVD Panel 1 Human CVD Panel HCVD1MAG-67K) (Cat. No. Cardiovascular ) MSHMAG-21K (Cat. No. Multi-Species Hormone Multi-Species ) HPTP1MAG-66K (Cat. No. Human Pituitary 1 Panel Human IGF ) HIGFMAG-52K (Cat. No. Human IGF Binding Protein Human IGF Binding ) HIGFBMAG-53K (Cat. No. ) HLPPMAG-57K (Cat. No. Human Liver Protein Human Liver Human Aging Panel 1 Panel Aging Human ) HAGE1MAG-20K (Cat.No. FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. MILLIPLEX® map CANCER / NEUROSCIENCE / TOXICITY

HUMAN 10 |EMDMILLIPORE.COM/MILLIPLEXMerckMillipore.com/milliplex (Cat. No.(Cat. HCCBP3MAG-58K) Biomarker Panel 3 Human CirculatingCancer No.(Cat. HCCBP2MAG-58K) Biomarker Panel 2 Human CirculatingCancer (Cat. No.(Cat. HANG2MAG-12K) Human AngiogenesisPanel 2 No.(Cat. HAGP1MAG-12K) Factor Panel 1 Human Angiogenesis/Growth No.(Cat. HCMBMAG-22K) Biomarker Panel 1 Human Cancer/Metastasis No.(Cat. HCCB4MAG-58K) Biomarker Panel 4 Human CirculatingCancer sHGFR/sc-Met sHER3/sEGFR3/ sHER2/sEGFR2/ sEGFR/sHER1/sErbB1 sE-Selectin sc-Kit/sStem Cell sCD31/sPECAM-1 sAXL Angiostatin/Kringle G-CSF Follistatin (FST) FGF-2/FGF-basic FGF-1/FGF-acidic Endothelin-1 Endoglin EGF BMP-9 Angiopoietin-2 Osteonectin/SPARC Neuron-specific GDF-15 DKK1 Hepsin EpCAM CD44 AnhydraseCarbonic ALDH1A1 IGFBP3 Galectin-3 Fibroblast Activation Ferritin D Cathepsin Complement Factor Antithrombin III sErbB3 sErbB2 (SCFR) Factor Receptor Enolase (NSE) 9 (CA9) Protein (FAP) H (CFH) sVEGFR3/sFlt-4 sVEGFR2/sKDR/sFlk-1 sVEGFR1/sFlt-1 suPAR sTIE-2 Thrombospondin-2 Tenascin-C (TN-C) PDGF-AB/BB Osteopontin (OPN) sNeuropilin-1 (sNRP-1) sIL-6Rα VEGF-D VEGF-C VEGF-A Growth Placental Leptin IL-8/CXCL8 HGF HB-EGF YKL40/CHI3L1 TWEAK TRAP5 Periostin Osteoprotegerin Transglutaminase 2 NCAML1/L1CAM/ Midkine Mesothelin Kallikrein-6 Sex HormoneBinding Myeloperoxidase Melanoma Inhibitory DBinding Vitamin Matrix Extracellular (TSP-2) Factor (PLGF) (OPG) (TGM2) CD171 Globulin (SHBG) (MPO) (MIA) Activity Protein Protein 1(ECM1) (Cat. No.(Cat. HND3MAG-39K) PanelDisorders 3 Human Neurological Disease Panel 1 Human Neurodegenerative (Cat. No.(Cat. HNDG4MAG-36K) samples) (CSF Disease Panel 4 Human Neurodegenerative No.(Cat. HNDG3MAG-36K) Disease Panel 3 Human Neurodegenerative No.(Cat. HNDG2MAG-36K) Disease Panel 2 Human Neurodegenerative No.(Cat. HNDG1MAG-36K) (Cat. No.(Cat. HNS2MAG-95K) Human NeurosciencePanel 2 (Cat. No.(Cat. HNS1MAG-95K) samples) (CSF Human NeurosciencePanel 1 No.(Cat. HNABTMAG-68K) samples) (CSF Human Amyloid andTau Beta Neuroscience Kallikrein-6 Fetuin A Contactin-1 Angiotensinogen GDNF Amyloid 1-42 beta Amyloid 1-40 beta sNCAM Myeloperoxidase sICAM-1 D Cathepsin BDNF Ferritin FABP3 ApoE4 Angiogenin (ANG) Neuron-specific Acidic Glial Fibrillary α-Synuclein CRP C4 α-1-Antitrypsin (A1AT) Apo E Apo CIII Apo AI α-2-Macroglobulin Amyloid 1-42 beta Amyloid 1-40 beta (AGT) (MPO) Enolase (NSE) Protein (GFAP) ◦ ◦ ◦ Soluble Superoxide Soluble Superoxide Soluble Superoxide Osteopontin (OPN) S100B sRAGE sVCAM-1 RANTES/CCL5 PDGF-AB/BB PDGF-AA PAI-1 (total) TREM2 PRNP (PrionProtein) Neurogranin (NRGN) Transglutaminase 2 PARK7/DJ1 PARK5/UCHL1 Serum Amyloid P PEDF MIP-4/PARC/CCL18 Complement Factor Complement Factor Tau (total) pTau (Thr181) Dismutase 2 (sSOD2) Dismutase 1(sSOD1) Dismutase (TGM2) (SAP) H (CFH) C3

(Cat. No.(Cat. HKI2MAG-99K) (Urine samples) PanelHuman Kidney Injury 2 No.(Cat. HKI1MAG-99K) (Urine samples) PanelHuman Kidney Injury 1 Toxicity No.(Cat. HNCSMAG-35K) Human CircadianStress (Cat. No.(Cat. HLINJMAG-75K) Human Liver Injury No.(Cat. HKI6MAG-99K) (Serum/Plasma samples) PanelHuman Kidney Injury 6 No.(Cat. HKI5MAG-99K) (Serum/Plasma samples) PanelHuman Kidney Injury 5 No.(Cat. HKI4MAG-99K) (Serum/Plasma samples) PanelHuman Kidney Injury 4 No.(Cat. HKI3MAG-99K) (Urine samples) PanelHuman Kidney Injury 3 (Cat. No.(Cat. HNPMAG-35K) Human Neuropeptide IP-10/CXCL10 GSTα FABP1 Collagen IV Calbindin Cortisol Neurotensin β-Endorphin α-MSH GSTα ARG1 5'NT/CD73 Clusterin β-2-Microglobulin NGAL/Lipocalin-2 Collagen IV α-1-Microglobulin KIM-1 IP-10/CXCL10 FABP1 EGF RBP4 β-2-Microglobulin Cystatin C Clusterin Albumin α-1-Microglobulin TIMP-1 TFF-3 Renin Osteoactivin KIM-1 Melatonin P Substance Oxytocin SDH MDH1 RBP4 Cystatin C Uromodulin TIMP-1 Osteoactivin Renin PTH Osteopontin (OPN) Uromodulin Osteopontin (OPN) NGAL/Lipocalin-2 EGF ■

^ ■

^ MILLIPLEX® map IMMUNOLOGY / METABOLISM / ENDOCRINOLOGY FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.

NHP / MOUSE (mature) (LTA) IL-17A/F IL-20 MDC/CCL22 MCP-5/CCL12 MIP-3α/CCL20 MIP-3β/CCL19 TARC/CCL17 TIMP-1 IL-17E/IL-25 IL-17F IL-21 IL-22 IL-23 IL-27 IL-28B/IFNλ3 IL-31 IL-33/NF-HEV MIP-3α/CCL20 TNFα TNFβ/Lymphotoxin-α IL-4 IL-5 IL-6 IL-10 IL-13 MIP-1β/CCL4 RANTES/CCL5 TNFα IL-10 (p70) IL-12 IL-13 IL17A/CTLA8 KC/GROα/CXCL1 LIX MCP-1/CCL2 MIP-2/CXCL2 TNFα ) MECY2MAG-73KPX ) MHSTCMAG-70KPMX MerckMillipore.com/milliplex | 11 MerckMillipore.com/milliplex ) MECY2MAG-73K (Cat.No. ) MTH17MAG-47K (Cat. No. ) MCD8MAG-48K (Cat. No. ) MECY2MAG73KPXBK (Bulk Cat. No. (Cat. No. MT17MAG47K-PX25) (Cat. No. MT17MAG47PMX25BK) (Bulk Cat. No. MCD8MAG48K-PX15) (Cat. No. ) MCD8MAG48KPX15BK (Bulk Cat. No. ) MHSTCMAG-70KPXBK (Bulk Cat. No. (Cat. No.

(Cat. No. CCL21/6Ckine TNFRSF9 Serum/Plasma only samples only Tissue Culture format assay Competitive Available for custom premix Available sample a different Analyte requires dilution from others in panel

Erythropoietin (EPO) Exodus-2/ Fractalkine/CX3CL1 IFNβ1 IFNγ IL-11 IL-16 sCD40L GM-CSF IFNγ IL-1β IL-2 IL-4 IL-5 IL-6 IL-10 (p70) IL-12 IL-13 IL-15 IL-17A/CTLA8 sCD137/4-1BB/ sFas/TNFRSF6 sFasL GM-CSF Granzyme B IFNγ IL-2 GM-CSF IFNγ IL-1α IL-1β IL-2 IL-4 IL-5 IL-6 IL-7 15 15 25 25 15 15 18 18 Mouse Cytokine/Mouse 2 Panel Chemokine Mouse High Sensitivity T Cell ) MHSTCMAG-70K (Cat. No. Mouse Th17 Mouse CD8+ T Cell

EMDMILLIPORE.COM/MILLIPLEX | 11 EMDMILLIPORE.COM/MILLIPLEX ● ★ ^ ◦ ♦ ♦ ♦ ♦ ♦ ♦ ♦ ♦ ♦ ♦ ♦ ♦ ♦ Polypeptide (PP) Polypeptide IL-13 IL-15 IL-17A/CTLA8 IP-10/CXCL10 KC/GROα/CXCL1 LIF LIX MCP-1/CCL2 M-CSF MIG/CXCL9 MIP-1α/CCL3 MIP-1β/CCL4 MIP-2/CXCL2 RANTES/CCL5 TNFα VEGF-A Insulin Leptin MCP-1/CCL2 Pancreatic (total)PYY Testosterone T3 T4 FSH GH LH TSH ◗ ◗ ◗ ) MCYTOMAG-70K-PMX ♦ ♦ ◗ ♦ ♦ ♦ ♦ ♦ ♦ ♦ ♦ ♦ ♦ ♦ ♦ These analytes cannot be plexed withThese analytes cannot be plexed other analytes in this panel in serum/ plasma a protease inhibitor during Requires sample collection sample extraction Requires No standard/QC; autoantibody levels as MFIs reported are ) MCYTOMAG-70K (Cat. No. ) MCYTMAG70PMX25BK (Bulk Cat. No. (Cat. No. ) MCYTMAG-70K-PX32 (Cat. No. ) MCYTMAG70PMX32BK (Bulk Cat. No. Protein (AgRP)

GM-CSF IFNγ IL-1α IL-1β IL-2 IL-3 IL-4 IL-5 IL-6 IL-7 IL-9 IL-10 (p40) IL-12 (p70) IL-12 Eotaxin/CCL11 G-CSF GIP (total) (active) GLP-1 Glucagon IL-6 Cortisol Estradiol Progesterone (active) Amylin C-Peptide (active) Ghrelin ACTH Agouti-Related CNTF ▲  ◗ ■ ** 25 25 32 32 Immunology Mouse Cytokine/Chemokine 1 Panel Multi-Species Hormone ■ ^ Multi-Species ) MSHMAG-21K (Cat. No. MOUSE ) NHPMHMAG-45K (Cat.No. PituitaryNon-Human Primate 1 Panel ) NHPPT1MG-46K (Cat. No. Metabolism/ Endocrinology Primate Non-Human Metabolic Hormone map kits ▼ ® A) (mature) (LT TGFβ3 IL-17E/IL-25 IL-21 IL-22 IL-23 IL-28A/IFNλ2 IL-31 IL-33/NF-HEV IP-10/CXCL10 MIP-3α/CCL20 Perforin RANTES/CCL5 ▲ TNFβ/Lymphotoxin-α IL-12/23 (p40) IL-12/23 IL-13 IL-15 IL-17A/CTLA8 IL-18 MCP-1/CCL2 MIP-1α/CCL3 MIP-1β/CCL4 TGFα TNFα VEGF-A

) PRCYTMAG40K-PX24 ) PRCYTMAG40K-PX25 ) PCYTMG-40K-PX23 Analytes which cannot be plexed together: and total – Active and total – Free listed in Cat. No. Available Premix panel only Premix β1

♦ ▼ Legend key for MILLIPLEX key Legend † Bulk indicates Space Saver Packaging Bulk indicates Space Saver ) PRCYT2MAG40K (Cat. No. ) PRCYTOMAG-40K (Cat.No. ) PRCY2MG40PMX24BK (Bulk Cat. No. ) PRCY2MG40PMX25BK (Bulk Cat. No. ) PRCYMAG40PMX23BK (Bulk Cat. No. (Cat. No. (Cat. No. (Cat. No. TNFRSF9

TGF TGFβ2 TGFβ1 Eotaxin/CCL11 sFasL FGF-2/FGF-basic Fractalkine/CX3CL1 Granzyme A Granzyme B IL-1α IL-2 IL-4 IL-6 IL-16 IL-17A/CTLA8 sCD137/4-1BB/ sCD40L G-CSF GM-CSF IFNγ IL-1β IL-1Ra IL-2 IL-4 IL-5 IL-6 IL-8/CXCL8 IL-10 NON-CONFIGURABLE KIT 24 25 25 24 23 23 Multi-Species TGFβ – 3 Plex TGFβ Multi-Species ) TGFBMAG-64K-03 (Cat. No. ) TGFBMAG-64K-01 (Cat. No. ) TGFBMAG-64K-01BK (Bulk Cat. No. Multi-Species TGFβ – TGFβ Multi-Species Singleplex Non-Human Primate Cytokine/Chemokine 2 Panel Non-Human Primate Non-Human Cytokine/Chemokine 1 Panel PRIMATE Immunology FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. MILLIPLEX® map IMMUNOLOGY / IMMUNE RESPONSE / METABOLISM / ENDOCRINOLOGY / CVD / BONE / CANCER

MOUSE 12 |EMDMILLIPORE.COM/MILLIPLEXMerckMillipore.com/milliplex (Cat. No.(Cat. MMMP1MAG-79K) (Serum/Plasma samples) Mouse MMPPanel 1 Receptor Mouse SolubleCytokine (Cat. No.(Cat. MGAMMAG-300E) Mouse IgE –Singleplex Isotyping Mouse Immunoglobulin Immune Response No.(Cat. TGFBMAG-64K-03) Multi-Species TGFβ –3Plex No.(Bulk Cat. TGFBMAG-64K-01BK) No.(Cat. TGFBMAG-64K-01) Singleplex Multi-Species TGFβ – No.(Cat. MMMP3MAG-79K) culture(Cell samples) Mouse MMPPanel 3 No.(Cat. MMMP2MAG-79K) (Serum/Plasma samples) Mouse MMPPanel 2 NON-CONFIGURABLE KIT IgE IgG2a IgG1 IgA TGFβ2 TGFβ1 TGFβ1 MMP-8 MMP-3 MMP-2 proMMP-9 MMP-3 MMP-2 sIL-6R sIL-4R sIL-2Rα sIL-1RII sIL-1RI sGP130 sCD30

(Cat. No.(Cat. MGAMMAG-300K) No.(Cat. MSCRMAG-42K) IgM IgG3 IgG2b TGFβ3 MMP-12 proMMP-9 MMP-12 MMP-8 sVEGFR3/sFlt-4 sVEGFR2/sKDR/sFlk-1 sVEGFR1/sFlt-1 sTNF RII sTNF RI sRAGE ▼ (Cat. No.(Cat. MPTMAG-49K) Mouse Pituitary (Cat. No.(Cat. MMYOMAG-74K) Mouse Myokine (Cat. No.(Cat. MADPNMAG-70K-01) (Serum/Plasma samples) Singleplex Mouse Adiponectin– No.(Cat. MADCYMAG-72K) culture(Cell samples) Mouse Adipocyte No.(Cat. MADKMAG-71K) (Serum/Plasma samples) Mouse Adipokine Endocrinology Metabolism/ (Cat. No.(Cat. MMHMAG-44K) Hormone Mouse Metabolic GH FSH BDNF ACTH IL-15 IL-6 Follistatin-like Fractalkine/CX3CL1 FGF-21 (EPO)★ Erythropoietin BDNF Insulin IL-6 Glucagon GLP-1 (active) GIP (total) Ghrelin (active) C-Peptide 2 Amylin (active) Adiponectin MCP-1/CCL2 Leptin IL-6 Adiponectin MCP-1/CCL2 Leptin Insulin IL-6 Protein 1(FSTL1) ◗ ◗ ◗ ◗ TSH Prolactin LH Osteonectin/SPARC Osteocrin (OSTN)/ (OSM) Oncostatin-M Myostatin/GDF8 LIF Irisin TNFα Resistin PYY (total) Pancreatic MCP-1/CCL2 Leptin TNFα Resistin PAI-1 (total) TNFα Resistin PAI-1 (total) Musclin Polypeptide (PP) Mouse AcutePhasePanel 2 No.(Cat. MCVD2MAG-77K) PanelMouse CVD 2 No.(Cat. MCVD1MAG-77K) PanelMouse CVD 1 Cardiovascular No.(Cat. MSHMAG-21K) (Cat. No.(Cat. MAGPMAG-24K) Factor Panel 1 Angiogenesis /Growth Mouse Cancer No.(Cat. MBNMAG-41K) Mouse Bone Bone No.(Cat. MAP2MAG-76K) Multi-Species Hormone■ ^ LIGHT Follistatin (FST) Endocan-1 (ESM-1) CXCL16 sCD40L ProMMP-9 sICAM-1 sE-Selectin sCD31/sPECAM-1 Progesterone Estradiol Cortisol IL-1β HGF G-CSF Follistatin (FST) FGF-2/FGF-basic sFasL Endothelin-1 Endoglin EGF ▲ sCD31/sPECAM-1 ▲ Betacellulin Angiopoietin-2 ▲ Amphiregulin sALK-1 Insulin IL-6 FGF-23 DKK1 ACTH α-2-Macroglobulin α-1-Acid Glycoprotein Adipsin/Factor D (AGP) Troponin T(TnT) Troponin I(TnI) PLGF-2 M(OSM) Oncostatin Thrombomodulin sP-Selectin PAI-1 (total) T4 T3 Testosterone VEGF-D VEGF-C VEGF-A TNFα SDF-1/CXCL12 Prolactin Growth Placental MIP-1α/CCL3 MCP-1/CCL2 Leptin KC/CXCL1 IL-17A/CTLA8 IL-6 TNFα Sclerostin (SOST) Osteoprotegerin Leptin Serum Amyloid P Haptoglobin CRP Factor (PLGF-2) (OPG) (SAP) MILLIPLEX® map NEUROSCIENCE / TOXICITY / IMMUNOLOGY / METABOLISM / ENDOCRINOLOGY / CVD FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.

MOUSE / RAT ■ 1 (FSTL1) (MYL3) (vWF) LH Prolactin TSH Melatonin^ Testosterone T3 T4 FABP3 Protein Follistatin-like Light Chain 3 Myosin TIMP-1 IL-6 / MCP-1 CCL2 (total) PAI-1 TIMP-1 TNFα VEGF sICAM-1 Willebrand Factor von TSH MerckMillipore.com/milliplex | 13 MerckMillipore.com/milliplex (cTnI) (cTnT) Muscle (CKM) Growth Factor (CTGF) 1 / CXCL1 Serum/Plasma only samples only Tissue Culture format assay Competitive Available for custom premix Available sample a different Analyte requires dilution from others in panel ACTH BDNF FSH GH ACTH Corticosterone^ Cortisol Estradiol Progesterone Cardiac Troponin I Cardiac Troponin T Cardiac Troponin Kinase Creatine Caveolin-1 Tissue Connective / CINC- / KC GROα Adiponectin sE-Selectin T3^ T4^ Rat Pituitary ) RPTMAG-86K (Cat.No. Hormone Rat Stress ) RSHMAG-69K (Cat. No. Multi-Species Hormone ■ ^ Hormone Multi-Species ) MSHMAG-21K (Cat. No. Cardiovascular Rat Cardiac 1 Injury Panel ) RCI1MAG-87K (Cat. No. ) RV1MAG-26K (Cat. No. ) RV2MAG-26K (Cat. No. Rat Thyroid ) RTHYMAG-30K (Cat. No. Rat Vascular Injury 1 Panel Rat Vascular (Serum/Plasma samples) Injury 2 Panel Rat Vascular (Serum/Plasma samples)

EMDMILLIPORE.COM/MILLIPLEX | 13 EMDMILLIPORE.COM/MILLIPLEX ● ★ ^ ◦ ▼ Polypeptide (PP) Polypeptide Musclin / CCL2 MCP-1 (total) PAI-1 TNFα MCP-1/CCL2 (total) PAI-1 TNFα TGFβ3 Insulin Leptin MCP-1/CCL2 Pancreatic (total)PYY TNFα IL-6 IL-15 Irisin LIF Osteocrin (OSTN)/ Osteonectin/SPARC ◗ ◗ ◗ ◗ These analytes cannot be plexed withThese analytes cannot be plexed other analytes in this panel in serum/ plasma a protease inhibitor during Requires sample collection sample extraction Requires No standard/QC; autoantibody levels as MFIs reported are 1 (FSTL1) IL-1β IL-6 Insulin Leptin Adiponectin IL-1β IL-6 Leptin TGFβ1 TGFβ1 TGFβ2 (active) Amylin 2 C-Peptide (active) Ghrelin GIP (total) (active) GLP-1 Glucagon IL-6 BDNF Erythropoietin (EPO) FGF-21 Fractalkine/CX3CL1 Protein Follistatin-like Myostatin/GDF8 NON-CONFIGURABLE KIT ▲  ◗ ■ ** Rat Metabolic Hormone ) RMHMAG-84K (Cat. No. ) RADPKMAG-80K (Cat. No. Rat Adipocyte (Cell samples) culture ) RADPCMAG-82K (Cat. No. – 3 Plex TGFβ Multi-Species ) TGFBMAG-64K-03 (Cat. No. Metabolism/ Endocrinology Rat Adipokine (Serum/Plasma samples) Multi-Species TGFβ – TGFβ Multi-Species Singleplex ) TGFBMAG-64K-01 (Cat.No. ) TGFBMAG-64K-01BK (Bulk Cat. No. Rat Myokine ) RMYOMAG-88K (Cat. No. map kits ® ■ ^ IL-10 (p70) IL-12 IL-13 IL-17A/CTLA8 IL-18 IP-10/CXCL10 Leptin LIX MCP-1/CCL2 MIP-1α/CCL3 MIP-2/CXCL2 RANTES/CCL5 TNFα VEGF Renin TIMP-1 VEGF-A NGAL/Lipocalin-2 Osteopontin (OPN) Amyloid beta 1-42 Amyloid Oxytocin Substance P

) RECYMAG65K27PMX Analytes which cannot be plexed together: and total – Active and total – Free listed in Cat. No. Available Premix panel only Premix

♦ ▼ Legend key for MILLIPLEX key Legend † Bulk indicates Space Saver Packaging Bulk indicates Space Saver ) RECYTMAG-65K (Cat. No. ) RECYMAG65PMX27BK (Bulk Cat. No. (Cat. No. 1 / CXCL1

IFNγ IL-1α IL-1β IL-2 IL-4 IL-5 IL-6 EGF Eotaxin / CCL11 / CX3CL1 Fractalkine G-CSF GM-CSF / CINC- / KC GROα Clusterin C Cystatin EGF β-2-Microglobulin IP-10/CXCL10 KIM-1 α-MSH β-Endorphin Neurotensin Amyloid beta 1-40 Amyloid 27 27 Immunology Rat Cytokine/Chemokine RAT Mouse Kidney Injury Panel 2 InjuryMouse Kidney Panel ) MKI2MAG-94K (Cat. No. Toxicity 1 InjuryMouse Kidney Panel ) MKI1MAG-94K (Cat. No. Mouse Neuropeptide ) RMNPMAG-83K (Cat. No. ) MABMAG-83K (Cat.No. Neuroscience Beta Mouse Amyloid FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. MILLIPLEX® map BONE / NEUROSCIENCE / TOXICITY / IMMUNOLOGY / METABOLISM / ENDOCRINOLOGY

RAT / OTHER 14 |EMDMILLIPORE.COM/MILLIPLEXMerckMillipore.com/milliplex (Cat. No.(Cat. RBN1MAG-31K) (Serum/Plasma samples) Rat BonePanel 1 Bone No.(Cat. RV3MAG-26K) (Serum/Plasma samples) Rat Vascular Panel Injury 3 (Cat. No.(Cat. RLI1MAG-92K) Rat Liver Injury No.(Cat. RKTX2MAG-37K) (Urine samples) Rat Kidney Toxicity Panel 2 No.(Cat. RKTX1MAG-37K) (Urine samples) Rat Kidney Toxicity Panel 1 Toxicity No.(Cat. RMNPMAG-83K) Rat Neuropeptide Neuroscience GOT1 ARG1 5'NT/CD73 β-2-Microglobulin Albumin^ α-1-Acid Glycoprotein IP-10 /CXCL10 GSTα^ Clusterin Calbindin Neurotensin β-Endorphin α-MSH Insulin FGF-23 DKK1 ACTH α-2-Macroglobulin α-1-Acid Glycoprotein (AGP) (A2M) (AGP) SDH GSTα NGAL /Lipocalin-2 EGF Cystatin C VEGF-A TIMP-1 Osteopontin (OPN) KIM-1 P Substance Oxytocin Sclerostin (SOST) PTH Osteoprotegerin (OPG) Leptin Haptoglobin Fibrinogen ■ ^ ▲ (Bulk Cat. No.(Bulk Cat. TGFBMAG-64K-01BK) No.(Cat. TGFBMAG-64K-01) Singleplex Multi-Species TGFβ – Porcine Cytokine/Chemokine Feline Cytokine/Chemokine Equine Cytokine/Chemokine Cytokine/Chemokine Canine Immunology OTHER SPECIES 23 23 13 13 19 19 13 13 NON-CONFIGURABLE KIT IL-5 IL-4 IL-2 IL-1β IL-1α IFNγ GRO GM-CSF G-CSF Fractalkine/CX3CL1 FGF-2/FGF-basic Eotaxin/CCL11 TGFβ1 IL-4 IL-2 IL-1Ra IL-1β IL-1α IFNγ GM-CSF IL-12 (p40) IL-8 /CXCL8 IL-6 IL-4 IL-2 IL-1β IFNγ GM-CSF Flt3 Ligand sFAS/TNFRSF6 IL-10 IL-8 /CXCL8 IL-7 IL-6 IL-2 IFNγ GM-CSF

(Cat. No. (Cat. No. (Cat. No. (Cat. No. (Cat. (Bulk Cat. No.(Bulk Cat. PCYTMAG23PMX13BK) No.(Cat. PCYTMG-23K-13PX) No.(Bulk Cat. FCYTMAG20KPX19BK) (Bulk Cat. No.(Bulk Cat. CCYTMAG90KPX13BK) (Cat. No.(Cat. EQCYTMAG-93K) (Cat. No.(Cat. PCYTMAG-23K) (Cat. No.(Cat. CCYTOMAG-90K) EQCTMG93KPX23BK) EQCYTMG-93KPX23) FCYTMAG-20K-PMX) CCYTMG-90K-PX13) TNFα RANTES/CCL5 MCP-1/CCL2 IP-10/CXCL10 IL-18 IL-17A/CTLA8 IL-13 IL-12 (p70) IL-10 IL-8/CXCL8 IL-6 TNFα IL-18 IL-12 IL-10 IL-8/CXCL8 IL-6 TNFα SDF-1 /CXCL12 SCF RANTES /CCL5 PDGF-BB MCP-1 /CCL2 KC/GRO IL-18 IL-13 TNFα MCP-1 /CCL2 KC-like IP-10 /CXCL10 IL-18 IL-15 ▼ (Cat. No.(Cat. CKT2MAG-97K) (Urine samples) 2 Kidney ToxicityCanine Panel No.(Cat. CKT1MAG-97K) (Urine samples) Expanded Panel 1 Kidney ToxicityCanine Toxicity No.(Cat. MSHMAG-21K) Feline Hormone Metabolic No.(Cat. CPTMAG-96K) Pituitary Canine No.(Cat. CGTMAG-98K) GutHormone Canine Endocrinology Metabolism/ No.(Cat. TGFBMAG-64K-03) Multi-Species TGFβ –3Plex Multi-Species Hormone■ ^ No.(Cat. FMHMAG-29K) NON-CONFIGURABLE KIT β-2-Microglobulin Albumin KIM-1 IL-8 /CXCL8 Cystatin C Clusterin Progesterone Estradiol Cortisol FSH BDNF ACTH Glucagon GLP-1 (active) ◗ GIP (total) Ghrelin (active) Amylin ◗ (total) TGFβ2 TGFβ1 Glucagon GLP-1 (active) GIP (total) Ghrelin (active) Amylin (active) ◗ ◗ ◗ ◗ ◗ TFF-3 RBP4 Osteopontin (OPN) NGAL /Lipocalin-2 MCP-1 /CCL2 T4 T3 Testosterone TSH GH PYY (total) Pancreatic Leptin Insulin TGFβ3 PYY (total) Pancreatic Leptin Insulin Polypeptide (PP) Polypeptide (PP) ▼ CELL SIGNALING MILLIPLEX® map ASSAYS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.

CELL SIGNALING H, M, R H, M, R H, M, R H, M, R H, R H, M, R H, M, R H, M, R H, M, R H, M, R H, M, R

beads in solution Magnetic Beads Standard Controls QC1 & QC2 Phosphorylated Capture antibody-conjugated Capture antibody-conjugated NON-CONFIGURABLE KIT SAPE 11 Plex 3 3 3 3 3 3 3 3 3 3 3 MerckMillipore.com/milliplex | 15 MerckMillipore.com/milliplex – Total EMDMILLIPORE.COM/MILLIPLEX | 15 EMDMILLIPORE.COM/MILLIPLEX Streptavidin Phycoerythrin Streptavidin H Human M Mouse R Rat map Assays Assay Buffer Bead Diluent ® Analyte Akt/PKB GSK3α GSK3β IGF1R IR IRS1 mTOR p70S6 Kinase PTEN RPS6 TSC2 Akt/mTOR (Total) (Total) Akt/mTOR ▼ ) AB2 48-612MAG (Cat. No. map Cell Signaling portfolio to meet ® Uses Assay Buffer 1 Uses Assay Buffer 2 Uses Assay H, M, R H, M, R H, M, R H, M, R H, M H, R H H, M H, M, R H, M, R H, M, R 10X Wash Buffer 10X Wash Protocol Included AB1: AB2: kit? ® 11 Plex

– Serum Matrix 3 (Ser939) 3 (Ser235/236) 3 (Ser380) 3 (Thr389/412) 3 (Ser2448) 3 (Ser636) 3 (Tyr1162/1163) 3 (Tyr1135/1136) 3 (Ser9) 3 (Ser21) Phosphorylated 3 (Ser473) NON-CONFIGURABLE KIT Total Cocktail Detection Antibody Detection Antibody 96-well Plate Griener Black Mylar Plate Sealers Cannot plex with other phospho Akt Can be plexed with other 2 Plexes Can be plexed Premix panel only Premix

Phosphoprotein or total protein (or mixed) multiplex assays (≥ 3-plex) multiplex assays Phosphoprotein or total protein (or mixed) (2-plex) totalSame protein phosphoprotein and same assay protein in the own can plex on your kits you Singleplex MAPmate™

What’s in the MILLIPLEX in What’s ●● ✸ ▼ TSC2 RPS6 PTEN p70S6 Kinase mTOR mTOR IRS1 IR IGF1R GSK3β GSK3α Akt/PKB Analyte ▼ ) AB2 48-611MAG (Cat. No. Akt/mTOR (Phosphoprotein) Akt/mTOR We offer three kit formats in our MILLIPLEX offer three We Cell Signaling MILLIPLEX cells communicate how your Uncover 2. 3. your diverse needs: diverse your 1. FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. CELL SIGNALING MILLIPLEX® map ASSAYS

CELL SIGNALING 16 |EMDMILLIPORE.COM/MILLIPLEXMerckMillipore.com/milliplex (Cat. No.(Cat. 48-682MAG) AB1▼ Human Bcl-2Family ApoptosisPanel 1-6Plex No.(Cat. 48-669MAG) AB2▼ Early Apoptosis (Cat. No.(Cat. 48-660MAG) AB2▼ MAPK/SAPK (Phosphoprotein) No.(Cat. 48-621MAG) AB1▼ Human DNADamage/Genotoxicity No.(Cat. 48-683MAG) AB1▼ Human Bcl-2Family ApoptosisPanel 2-4Plex (interaction) NOXA/Mcl-1 Mcl-1 Bcl-xL Bcl-2 p53 JNK/SAPK1 Active 9 Caspase Active 8 Caspase Bcl-2 BAD Analyte ATF2 Analyte ATR Analyte Analyte (interaction) Mcl-1/BIM BIM (interaction) Bcl-xL/BAD BAX BAD Analyte Akt/PKB Chk1 c-Jun Erk/MAPK 1/2 Chk2 H2A.X HSP27 MDM2 JNK/SAPK1 p21 MEK1 p53 MSK1 p38/SAPK2A/B p53 STAT1 – 7Plex Total Total Total Total Total

3 3 3 3 3 3 3 3 3 3 3 3 3 3 NON-CONFIGURABLE KIT NON-CONFIGURABLE KIT NON-CONFIGURABLE KIT NON-CONFIGURABLE KIT NON-CONFIGURABLE KIT 3 (Thr71) Phosphorylated 3 (Ser73) 3 (Thr185/Tyr187) 3 (Ser78) 3 (Thr183/Tyr185) 3 (Ser222) 3 (Ser212) 3 (Thr180/Tyr182) 3 (Ser15) 3 (Tyr701) Phosphorylated 3 (Ser345) 3 (Thr68) 3 (Ser139) 3 (Ser15) 3 (Ser46) 3 (Thr183/Tyr185) 3 (Ser70) 3 (Ser112) 3 (Ser473) Phosphorylated 3 (Ser112) Phosphorylated Phosphorylated – 10Plex – 7Plex H, M H, M,R H, M,R H H, M,R H, M,R H, M H, M,R H H, M H H, M,R H H, M,R H, M,R H H H H, M,R H H H H H, M,R H H H H H H H H H Multi-Pathway (Phosphoprotein)—9Plex No.(Cat. HPRTKMAG-01K) AB1 thatMeetyour(Choose Analytes Needs) Human RTK (Phosphoprotein) (Cat. No.(Cat. 48-681MAG) AB2▼ Multi-Pathway (Total) —9Plex No.(Cat. 48-680MAG) AB2▼ c-Kit Analyte c-Met/HGFR ErbB1/EGFR ErbB2/HER2 ErbB3/HER3 ErbB4/HER4 FGFR1 Flt3 IGF1R IR MSCFR PDGFRα PDGFRβ TIE1 TIE2 VEGFR1/Flt-1 VEGFR2/KDR/Flk-1 VEGFR3/Flt-4 STAT5A/B STAT3 p70S6 Kinase p38/SAPK2A/B NFκB JNK/SAPK1 Erk/MAPK 1/2 Analyte Analyte CREB STAT5A/B STAT3 p70S6 Kinase p38/SAPK2A/B NFκB JNK/SAPK1 Erk/MAPK 1/2 CREB Akt/PKB Akt/PKB Total Total 3 3 3 3 3 3 3 3 3 Total NON-CONFIGURABLE KIT NON-CONFIGURABLE KIT 3 (Tyr694/699) 3 (Ser727) 3 (Thr389/412) 3 (Thr180/Tyr182) 3 (Ser536) 3 (Thr183/Tyr185) 3 (Thr185/Tyr187) 3 (Ser133) Phosphorylated 3 (Ser473) Phosphorylated (pan Tyr) Phosphorylated (pan Tyr) (pan Tyr) (pan Tyr) (pan Tyr) (pan Tyr) (pan Tyr) (pan Tyr) (pan Tyr) (pan Tyr) (pan Tyr) (pan Tyr) (pan Tyr) (pan Tyr) (pan Tyr) (pan Tyr) (pan Tyr) (pan Tyr) H, M,R H, M,R H, M,R H, M,R H H, M,R H, M,R H, M,R H, M,R H, M,R H, M,R H, M,R H, M,R H, M,R H, M,R H, M,R H, M,R H, M,R H H H H H H H H H H H H, M,R H H H H H H, M,R CELL SIGNALING MILLIPLEX® map ASSAYS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.

CELL SIGNALING H H, M, R H, M, R H, M, R H, M, R H, M, R H, M, R H, M, R H, M, R H H H, M, R H, M, R H H, M, R H H H, M, R H, M, R H, M, R H, M, R NON-CONFIGURABLE KIT NON-CONFIGURABLE KIT NON-CONFIGURABLE KIT NON-CONFIGURABLE KIT Phosphorylated (Ser473) Phosphorylated 3 (Ser473) Phosphorylated (Ser474) Phosphorylated (Ser472) 2 Plex 2 Plex 2 Plex Phosphorylated 3 (pan Tyr) 3 (Ser133) 3 (Thr185/Tyr187) 3 (pan Tyr) 3 (pan Tyr) 3 (pan Tyr) 3 (pan Tyr) Phosphorylated 3 (Ser473) 3 (Thr185/Tyr187) 3 (Ser465/467) 3 (Ser423/425) NON-CONFIGURABLE KIT NON-CONFIGURABLE KIT NON-CONFIGURABLE 2 Plex – – – MerckMillipore.com/milliplex | 17 MerckMillipore.com/milliplex – 3 3 3 3 Total Total Total Total 3 3 EMDMILLIPORE.COM/MILLIPLEX | 17 EMDMILLIPORE.COM/MILLIPLEX Total Total H Human M Mouse R Rat ) AB2 ▼ ✸ ●● 1MAG 3 Akt1 Akt1 Akt2 Akt/PKB Analyte Analyte Analyte Akt2 Analyte Akt3 Akt3 Analyte CD3ε CREB Erk/MAPK 1/2 LAT Lck Syk ZAP-70 Analyte Akt/PKB Akt/PKB Erk/MAPK 1/2 SMAD2 SMAD3 SMAD4 TGFβRII Akt Phospho/Total Akt Phospho/Total ▼ ✸ ●● ) AB2 48-618MAG (Cat. No. Akt1 Phospho/Total 48-6 (Cat. No. Akt2 Phospho/Total ) AB2 ▼ ✸ ●● 48-632MAG (Cat. No. Akt3 Phospho/Total ) AB2 ▼ ✸ ●● 48-633MAG (Cat. No. T-Cell Receptor (Phosphoprotein) — 7 Plex — 7 (Phosphoprotein) Receptor T-Cell ) AB2 ▼ 48-690MAG (Cat.No. — 6 Plex TGFβ ) AB2 ▼ 48-614MAG (Cat. No. Uses Assay Buffer 1 Uses Assay Buffer 2 Uses Assay H, M H H, M, R H, M, R H H H, R H, M, R H, M H, M, R H, R H, M, R H, M, R H, M, R H, M, R H, M, R H, M, R H, M, R H H H H H, M H AB1: AB2:

Phosphorylated 3 (Tyr701) 3 (Tyr690) 3 (Tyr705) 3 (Tyr694/699) 3 (Tyr641) (Tyr412) 3 (Tyr412) 3 (Tyr420) 3 (Tyr411) 3 (Tyr394) 3 (Tyr397) 3 (Tyr419) 3 (Tyr421) Phosphorylated 3 (Tyr389) 3 (Ser422) 3 (Ser209) 3 (Ser1108) Phosphorylated 3 (Ser51) 3 (Thr37/46) Phosphorylated 3 (Ser194) 3 (Ser32) 3 (Ser177/181) 3 (Ser536) NON-CONFIGURABLE KIT NON-CONFIGURABLE KIT NON-CONFIGURABLE KIT NON-CONFIGURABLE KIT NON-CONFIGURABLE

3 3 3 Total Total Total Total Cannot plex with other phospho Akt Can be plexed with other 2 Plexes Can be plexed Premix panel only Premix

●● ✸ ▼ STAT1 Analyte STAT2 STAT3 STAT5A/B STAT6 Analyte Fgr Fyn Hck Lck Lyn Src Yes Blk Analyte elF-4B elF-4E elF-4G 4E-BP1 Analyte elF2a c-Myc FADD FADD IκBα IKKα/β IKKα/β NFκB NFκB TNFR1 STAT (Phosphoprotein) — 5 Plex (Phosphoprotein) STAT ▼ ) AB2 48-610MAG (Cat. No. ▼ ) AB2 48-650MAG (Cat. No. Src Family Kinase Active Site Site Kinase Active Src Family (Phosphoprotein) — 8 Plex ) AB2 ▼ 48-655MAG (Cat. No. Protein Translation — 6 Plex Protein Translation NFκB — 6 Plex — 6 NFκB ) AB1 ▼ 48-630MAG (Cat.No. FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. CELL SIGNALING MILLIPLEX® map ASSAYS

CELL SIGNALING 18 |EMDMILLIPORE.COM/MILLIPLEXMerckMillipore.com/milliplex (Cat. No.(Cat. 48-619MAG) AB2▼●● Erk/MAPK 1/2Phospho/Total No.(Cat. 48-628MAG) AB2▼●● CREB Phospho/Total (Cat. No.(Cat. 48-622MAG) AB2▼●● JNK Phospho/Total No.(Cat. 48-626MAG) AB2▼●● IRS1 Phospho/Total Erk/MAPK 1/2 Analyte CREB Analyte JNK/SAPK1 Analyte IRS1 Analyte CREB Erk/MAPK 1/2 JNK/SAPK1 IRS1 ▼ ✸ ●●

Premix panelonly Can beplexedCan withother2Plexes Cannot plexwithotherphosphoAkt Cannot Total Total Total Total 3 3 3 3 – – – 2Plex 2Plex 2Plex 3 (Thr185/Tyr187) Phosphorylated 3 (Thr183/Tyr185) Phosphorylated 3 (Ser636) Phosphorylated NON-CONFIGURABLE KIT NON-CONFIGURABLE KIT NON-CONFIGURABLE KIT NON-CONFIGURABLE KIT 3 (Ser133) Phosphorylated

– 2Plex AB2: AB1: H, M,R H, M,R H, M,R H, M,R H, M,R H, M,R H, M,R H, M Uses Assay Buffer2 Uses Assay Buffer1 (Cat. No.(Cat. 48-623MAG) AB2▼●● STAT3 Phospho/Total No.(Cat. 48-624MAG) AB1orAB2▼●● p38 Phospho/Total No.(Cat. 48-625MAG) AB2▼●● mTOR Phospho/Total STAT3 Analyte p38/SAPK2A/B Analyte mTOR mTOR Analyte STAT3 p38/SAPK2A/B R Rat M Mouse H Human Total Total Total 3 3 3 – 2Plex – – 2Plex 3 (Ser2448) Phosphorylated Phosphorylated Phosphorylated 3 (Tyr705) 3 (Thr180/Tyr182) 2Plex NON-CONFIGURABLE KIT NON-CONFIGURABLE KIT NON-CONFIGURABLE KIT H, M H, M,R H, M,R H, M,R H, M,R H, M,R CELL SIGNALING MILLIPLEX® map ASSAYS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.

CELL SIGNALING Cat. No. 46-654MAG 46-655MAG 46-656MAG 46-612MAG 46-610MAG 46-685MAG 46-686MAG 46-618MAG 46-613MAG 46-609MAG 46-602MAG 46-604MAG 46-694MAG 46-675MAG 46-677MAG 46-667MAG 46-713MAG H, M H, M H H, M, R H, M, R H, M, R H, M, R H, M H, M H, M, R H, M, R H, M H, M H, M, R H, M, R H H, M, R Species Homology MerckMillipore.com/milliplex | 19 MerckMillipore.com/milliplex EMDMILLIPORE.COM/MILLIPLEX | 19 EMDMILLIPORE.COM/MILLIPLEX STAT1 (total) STAT1 STAT1 (Tyr701) STAT1 Cleaved PARP (total) PARP Cleaved p38/SAPK2A/B (total) p38/SAPK2A/B (Thr180/Tyr182) mTOR (total) mTOR mTOR (Ser2448) mTOR JNK/SAPK1 (total) JNK/SAPK1 (Thr183/Tyr185) ERK/MAPK 1/2 (total) ERK/MAPK 1/2 (Thr185/Tyr187) Caspase 3 (Active) BAD (Ser112) BAD Akt/PKB (total) Akt/PKB (Ser473) GAPDH (total) β-Tubulin (total) β-Tubulin MAPmate™ Kits MAPmate™ MAPmate™ portfolio are available to build into to build into available portfolio are MAPmate™ our information on more For custom assays. contact program, your custom immunoassay at customassay@ or email us sales specialist . emdmillipore.com Cell Signaling Panels to enhance Panels map Cell Signaling ® Phospho-specific and total MAPmate™ Phospho-specific and total MAPmate™ pairs can MAPmates™ GAPDH and β-Tubulin of the be used for normalization with any MAPmates™ • • Consult the protocol prior to use Consult the protocol Buffer & Detection Kit Use the Cell Signaling ), which contains all necessary (48-602MAG assay the MAPmate™ except reagents use Cell Signaling Assay assays All MAPmate™ Buffer 2 (AB2) should not be assays The following MAPmate™ together: plexed to order available are lysates Note: additional upon request Plex up to 8 individual MAPmate™ assays assays 8 individual MAPmate™ Plex up to Buffer and using our Cell Signaling together in existing Kit, or include them Detection MILLIPLEX Cell Signaling MAPmate™ Assays Cell MAPmate™ Signaling the panel within the guidelines provided provided within the guidelines the panel All of the analytes in the in the protocols. Important MAPmate™ Guidelines Important MAPmate™ • • • • • FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. MILLIPLEX® map CUSTOM IMMUNOASSAYS AND SERVICES

CUSTOM 20 |MerckMillipore.com/customassay services: assays designedforYOUR samples. We offerthefollowing • • • • • Accelerate your research withcustomizedMILLIPLEX Custom MILLIPLEX Get started today! Get started Kit bridgingforlot-to-lot reproducibility Design andvalidate assays onyour samples Rearrange intonewpanels existinganalytes withexistinganalytes Multiplex newanalytes Develop newanalytes feasibility [email protected] Place purchase order and participate inprojectkickoffPlace purchaseorderandparticipate call Submit fullproteinnames, for speciesandsampletype Receive fromsalesspecialist work planandquotation ® Assay Development mapAssay STEP 1 STEP 3 STEP 2 ® map MILLIPLEX® map CUSTOM IMMUNOASSAYS AND SERVICES FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.

CUSTOM MerckMillipore.com/customassay | 21 MerckMillipore.com/customassay ELISA Plate Reader Download our new custom menu of services menu of our new custom Download services listing of for a complete at brochure MerckMillipore.com/customassay Single analyte detection customized. of single analytes detection require assays When your and services for SMCxPRO™ consider custom assay only, sub-picogram detection Leverage ELISA plate readers. to quantify previously proteins capabilities of SMCxPRO™ information on more For undetectable in samples. visit MerckMillipore.com/smcxpro. SMCxPRO™, 9/18/18 1:54 PM Assays map Assays ® The life science business The life Darmstadt, of Merck KGaA, operates as Germany in the MilliporeSigma and Canada. U.S. Develop new analytes Develop Multiplex new analytes with existing analytes existing analytes into Rearrange new panels on assays Design and validate samples your Kit bridging for lot-to-lot consistency Dream it. Multiplex it. with Research Accelerate Your Customized MILLIPLEX • • • • • Designed for YOUR Samples Designed for YOUR 2018-14941_Custom Assay Brochure.indd 1 SMCxPRO™ FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. MILLIPLEX® map CUSTOM IMMUNOASSAYS AND SERVICES

CUSTOM 22 |EMDMILLIPORE.COM/MILLIPLEXMerckMillipore.com/milliplex Custom MILLIPLEX α-1 Microglobulin (A1AT)...... α -1-Antitrypsin (AGP). α 1-Acid Glycoprotein A 6Ckine/CCL21/Exodus-2 5’-NT/CD73 4E-BP1(Thr37/46) (total) 4E-BP1 4–6 MILLIPLEX ADAMTS13 ADAM15 9(total) Caspase Active 8(total) Caspase Active Active Caspase 3 ACTH α -Synuclein ...... α -MSH (AFP)...... α -Fetoprotein α -2 Macroglobulin (A2M)..... α -2 Macroglobulin VE Cadherin/Cadherin 5 VE Cadherin/Cadherin Transferrin sTM (SolubleThrombomodulin) ST2 (Suppressor ofTumorigenicity 2) Semaphorin 3A Lipocalin like Dsynthase prostaglandin (LPGDS) Lecithin–cholesterol Acyltransferase (LCAT) GRP78 Galectin-1 Elafin (CystatinCSTB B) Collagen 1 Ceruloplasmin CD13 AminopeptidaseN Calprotectin B-cell MaturationAntigen(BCMA) α 1-Acid Glycoprotein (AGP) Analyte Human Analyte Vitronectin Vasopressin TRAIL Tenascin C sAXL Periostin IGF-1 HGFR/cMET Galectin-3 Galectin-1 BMP-9 Analyte Mouse and/orRat ...... 9, 11, 12, 13, 14 ...... 9, 10, 12, 14 ® 10, 13, 14 Analyte Index mapAnalyte 9, 12, 14 10, 14 10, Page 7, 11 7, 10 10 10 16 16 19 17 17 9 9 9 ALDH1A1 Albumin (total) Akt3 (Ser472) Akt3 (total) Akt2 Amphiregulin Akt2 (Ser474) Akt2 (total) Akt1 (Ser473) Akt1 (total) Akt/PKB (Ser473) Akt/PKB Agouti-Related Protein (AgRP) Adipsin/Factor D Adipsin/Factor Adiponectin Angiogenin (ANG) Angiogenin 1-42 1-42) (Aβ beta ...... Amyloid 1-40 1-40) (Aβ ...... beta Amyloid (total) Amylin (active)...... Amylin Angiopoietin-2 Analyte ® ...... mapAnalytes ...... Serum/Plasma Urine Serum/Plasma Serum/Plasma Cell andTissueCulture Supernatants Urine Serum/Plasma Serum/Plasma Serum/Plasma Serum/Plasma Serum/Plasma Multiple Urine Serum/Plasma Serum/Plasma Serum/Plasma Urine Sample Serum/Plasma Plasma Serum/Plasma Serum/Plasma Serum/Plasma Serum/Plasma Serum/Plasma Serum/Plasma Serum/Plasma Serum/Plasma Serum/Plasma Sample ...... Human pp. 7-10; Primatep. 11;Mousepp. 11-13; Ratpp. 13-14; 8, 11, 12, 13, 14 15, 16, 17, 19 ...... 15, 16, 17 8, 12, 13 12, 8, 8, 9, 12 10, 13 10, 13 10, 10, 12 10, 10, 14 10, Page 8, 14 8, 9, 11 9, Other Species p. 14;CellSignaling pp. 15-19. 12 10 10 17 17 17 17 17 17 ATR (total) ATR ApoE4 ...... E) E (Apo Apolipoprotein (Apo CIII CIII) Apolipoprotein APRIL/TNFSF13 ATF2 (Thr71) ARG1 Apolipoprotein CII (Apo CII) B) B(Apo ...... Apolipoprotein ...... AII) (Apo AII Apolipoprotein ...... AI) (Apo AI Apolipoprotein Angiostatin/Kringle Angiotensinogen (AGT) ANGPTL6/AGF Antithrombin III Antithrombin Apelin ANGPTL4 ANGPTL3 Analyte ...... 10, 14 10, Page 9, 10 9, 10 9, 9, 10 9, 10 16 16 10 10 10 9 7 9 9 8 9 9 9 MILLIPLEX® map ANALYTE INDEX FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.

MILLIPLEX® map 9 9 9 9 7 9 7 8 9 7 9 9 8 9 9 8 8 9 9 14 17 17 17 19 16 16 16 10 10 10 10 10 16 10 15 15 15 15 13 7, 14 7, 14 7, 11 9, 14 9, 10 Page 10, 14 12, 13 ...... 7, 8, 11 8, 7, 9, 12, 14 14 12, 9, 9, 10, 12 12 10, 9, 9, 12, 14 12, 9, 8, 9, 10, 12 10, 9, 8, ...... 7, 11, 13, 14 13, 11, 7, ...... 9, 11, 12, 13, 14 13, 12, 11, 9, 9, 11, 12, 13, 14 13, 12, 11, 9, 8, 11, 12, 13, 14 13, 12, 11, 8, 14 13, 12, 11, 8, 14 13, 12, 11, 8, 8, 11, 12, 13, 14 13, 12, 11, 8, ...... 7, 8, 11, 12, 13, 14 13, 12, 11, 8, 7, ...... 7, 10, 11, 12, 13, 14 13, 12, 11, 10, 7, ...... MerckMillipore.com/milliplex | 23 MerckMillipore.com/milliplex ...... Analyte Fyn (Tyr420) Fyn Granzyme A...... Granzyme B...... Ghrelin...... (active) GIP (total) GITR GITRL Glial Fibrillary Acidic Protein..... (GFAP) ...... (active) GLP-1 GLP-1 (total) GRO GROα/KC/CINC-1/CXCL1 GH Flt3 Ligand Flt3 (panTyr) (FST) Follistatin FSH (autoantibody) Galectin-1 Galectin-3 Galectin-3 (autoantibody) GAPDH (total) GDF-11 GDF-15 GDNF H H2A.X (Ser139) Haptoglobin HB-EGF HCC-1/CCL14 HCC-4/CCL16 HCGβ Hck (Tyr411) HE4 (autoantibody) HIF-1α HMGB1 HSP27 (Ser78) Fibroblast Activation Protein (FAP) Protein Fibroblast Activation Fibronectin 1 Protein Follistatin-like (FSTL1) Fractalkine/CX3CL1 G G-CSF Glucagon GM-CSF GnRH GOT1 GSK3α (total) (Ser21) GSK3α GSK3β (total) (Ser9) GSK3β GSTα Hepsin (autoantibody) HER2/ErbB2 HGF HSP60 HSP70 Human Serum Albumin (HSA) HVEM FGF-23 (panTyr) FGFR1 Fgr (Tyr412) Fibrinogen EMDMILLIPORE.COM/MILLIPLEX | 23 EMDMILLIPORE.COM/MILLIPLEX 9 9 9 9 9 7 7 9 9 7 8 8 8 8 8 9 9 17 17 17 17 17 16 16 16 16 10 10 10 10 10 13 13 13 13 13 7, 9 7, 11 9,10 9,10 9, 12 8, 10 Page 10, 12 10, 12 16, 18 7, 9, 12 12 9, 7, ...... 16, 17, 18 18 17, 16, 16, 18, 19 18, 16,

10, 13, 14 13, 10, 8, 9, 10, 13 10, 9, 8, 8, 9, 12, 13 12, 9, 8, 7, 11, 13, 14 13, 11, 7, 9, 9, 10, 12, 14 12, 10, 9, 8, 11, 12, 13 13 12, 11, 8, 16, 17, 18, 19 18, 17, 16, 7, 10, 12, 13, 14 13, 12, 10, 7, 9, 11, 12, 13, 14 13, 12, 11, 9, ...... 7, 9, 10, 11, 12, 14 12, 11, 10, 9, 7, ...... 8, 9, 10, 11, 12, 13, 14 13, 12, 11, 10, 9, 8, ...... Analyte FABP4 DPP4 FABP3 F FABP1 DKK1 dPAPP-A D D-dimer E EGF eIF-4B (Ser422) eIF-4E (Ser209) Endothelin-1 (autoantibody) ENO1 Eotaxin-2/CCL24/MPIF-2 Eotaxin-3/CCL26 eIF-4G (Ser1108) eIF2a (Ser51) ENA-78/CXCL5 Endocan-1 (ESM-1) Endoglin Endostatin FGF-2/FGF-basic FGF-21 FGF-19 FGF-1/FGF-acidic Fetuin A...... FADD (Ser194) Ferritin Erythropoietin (EPO) (panTyr) ErbB1/EGFR (panTyr) ErbB2/HER2 ErbB3/HER3 (panTyr) ErbB4/HER4...... (panTyr) Erk/MAPK 1/2 (Thr185/Tyr187) Estradiol Extracellular Matrix Protein 1 (ECM1) Eotaxin/CCL11 EpCAM ...... Erk/MAPK 1/2 (total)...... Exodus-2/CCL21/6Ckine Complement C5a Complement C9 Complement B Factor Complement Complement Factor H Factor Complement Complement Factor I Factor Complement Connective Tissue Growth Factor (CTGF) CTACK/CCL27 CTAG1B/NY-ESO-1 (autoantibody) CTAG1B/NY-ESO-1 Contactin-1 Corticosterone Creatine Kinase Muscle (CKM) CREB (Ser133) CREB (total) Cortisol CTLA-4/CD152 Cardiac Troponin I (cTnI) ...... Cardiac Troponin T (cTnT) CXCL16 CYFR A21-1 Cystatin C Cystatin 9 7 8 7 7 9 9 7 9 9 9 9 9 9 9 9 9 9 8 8 8 8 8 8 9 9 8 8 9 17 17 17 19 16 16 16 16 16 16 16 16 16 16 10 19 10 10 16 16 10 10 13 12 9,11 8, 11 8, 10 Page 10, 14 16, 19 12, 13 9, 10, 12 10, 9, ...... Analyte map (continued) Index 10, 13, 14 13, 10, 10, 13, 14 13, 10, 10, 13, 14 14 13, 10, ...... ® ...... 8, 10, 12, 13, 14 13, 12, 10, 8, ...... Analyte C-Peptide 2 C-Reactive Protein. (CRP) BRAK/CXCL14 BTLA CA125 Bcl-2 (Ser70) BNP β2-Glycoprotein (autoantibody)...... Betacellulin β -Tubulin (total)...... β -Tubulin (total) BAD BAX (total) BCA-1/CXCL13 (total) Bcl-2 BIM Blk (Tyr389) BMP-9 BAD (Ser112) BAD BAFF/Blys CD27 CCL27 CCL28 CCNB1/Cyclin (autoantibody) B1 . Cathepsin D Cathepsin Caveolin-1 (CAV-1) Carbonic Anhydrase. 9 (CA9) Cadherin 13 (CDH13) Calbindin c-Kit (panTyr) c-Met/HGFR (panTyr) c-Myc (total)...... C-Peptide CA15-3 CA19-9 C c-Jun (Ser73) Bcl-xL (total) Bcl-xL Bcl-xL/BAD (interaction) BDNF B -2-Microglobulin β -Endorphin β MILLIPLEX CD28 Human pp. 7-10; Primate p. 11; Mouse pp. 11-13; Rat pp. 13-14; 11-13; Rat pp. 11; Mouse pp. 7-10; Primate p. Human pp. 15-19. pp. 14; Cell Signaling Other Species p. Complement C1q (autoantibody) Complement C1q CD86/B7-2 CEA CD3ε (panTyr) CD3ε CD40 CD44 CD80/B7-1 Complement C4b C5 Complement Complement C3 Complement Complement C3b / iC3b...... C4 Complement Complement C2 Complement Collagen IVCollagen CNTF Cleaved PARP (total)...... Clusterin CK-MB Chk1 (Ser345) Chk1 (Thr68) Chk2 Chromogranin-A (CHGA/CGA) CENP-B (Centromere Protein B) (autoantibody) CENP-A (Centromere Protein A) (autoantibody) FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. MILLIPLEX® map ANALYTE INDEX

MILLIPLEX® map 24 |EMDMILLIPORE.COM/MILLIPLEXMerckMillipore.com/milliplex IgG2b IgG2a IgG2 IgG1 IGFBP7 IGFBP6 IGFBP5 IGFBP4 IGFBP3 IGFBP2 IFNγ IFNβ1 IFNβ IFNα2 IgG4 IgG3 IL-20 IL-2 IL-11 IL-10 (Ser177/181) IKKα/β IgM IL-1β IL-1α IL-1Ra IL-17F IL-17E/IL-25 IGFBP1 IGF2BP3/IMP3 (autoantibody) IGF2BP2/IMP2 (autoantibody) (Tyr1135/1136) IGF1R (panTyr) IGF1R (total) IGF1R IGF1R IGF-2 IGF-1 IgE IgA ICOS IL-21 IL-3 /F IL-17A IL-16 IL-15 IL-14/α -Taxilin...... IL-13 (p40) IL-12/23 (p70) IL-12 (p40) IL-12 IL-12 IL-19 IL-18 I-309/CCL1 I MILLIPLEX I-TAC/CXCL11 IL-28B/IFNλ3 IL-28A/IFNλ2 IL-27 IL-23 IL-22 IL-32α IL-31 IL-29/IFNλ1 A8 TL /C IL-17A IL-33/NF-HEV (mature) IL-36β /IL-1F8 IL-36β IL-35 IL-24 IL-34 Analyte ...... 7, 8, 9, 11, 12, 13, 14 ...... 7, 8, 11, 12, 13, 14 ...... 7, 11, 12, 13, 14 7, 8, 11, 13, 14 7, 9, 11, 13, 14 7, 9, 11, 13, 14 7, 11, 13, 14 7, 11, 13, 14 7, 11, 13, 14 7, 11, 13, 14 ® ...... Analyte Index(continued) mapAnalyte 7, 11, 14 7, 11, 14 Page 8, 12 8, 8, 12 8, 8, 12 8, 8, 12 8, 12 8, 9, 10 9, 7, 11 7, 7, 11 7, 7, 11 7, 7, 11 7, 7, 11 7, 11 7, 11 7, 11 7, 7, 11 7, 11 7, 11 7, 11 7, 7, 11 7, 7, 11 7, 12 12 15 15 16 11 11 17 11 14 8 9 9 7 9 9 9 9 9 7 8 7 9 9 7 9 9 9 7 7 7 7 7 7 7 7 MDM2 (total) MDH1 MDC/CCL22 MSCFR (panTyr) MSCFR Jo-1 (autoantibody) (total)JNK/SAPK1 MCP-5/CCL12 MCP-4/CCL13 MCP-3/CCL7 MCP-2/CCL8 MCP-1/CCL2 LAG-3 (LTF)Lactotransferrin L Kallikrein-6 K (Thr183/Tyr185) JNK/SAPK1 Jag1 J Mcl-1 (total) Mcl-1/BIM (interaction) MCAM/MUC18/sCD146 (MBL) Lectin Mannose-Binding M-CSF M (Tyr397) Lyn Lymphotactin/XCL1 (LRG1) alpha-2-glycoprotein 1 Leucine-rich LIX/CXCL6/GCP-2 LIX LIGHT LH ...... (LIF) Factor Inhibitory Leukemia (LEPR)/OB-R Receptor Leptin Leptin LDLR (Tyr394) Lck (panTyr) Lck (panTyr) AT L KC/GROα /CXCL1 KC-like Protein Keratin-6 Keratin-1, 10 ...... Ku (autoantibody) KIM-1 IL-37/IL-1F7 IL-4 IL-38/IL-1F10 Irisin (Tyr1162/1163) IR (panTyr) IR (total) IR IP-10/CXCL10 Involucrin Insulin IL-9 IL-8/CXCL8 IL-7 IL-6 IL-5 IRS1 (total) IRS1 (Ser636) IRS1 IκBα (Ser32)IκBα ...... Analyte ...... 8, 9, 10, 11, 12, 13, 14 Human pp. 7-10; Primatep. 11;Mousepp. 11-13; Ratpp. 13-14; ...... 7, 8, 9, 11, 12, 13, 14 ...... 8, 9, 11, 12, 13, 14 7, 8, 11, 12, 13, 14 ...... 7, 8, 9, 10, 11, 14 7, 10, 11, 13, 14 7, 8, 11, 12, 13 . 9, 11, 12, 13 ... 7, 11, 13, 14 7, 11, 13, 14 ...... 16, 18, 19 16, 18, 19 10, 13, 14 8, 12, 13 7, 11, 14 15, 18 15, 15, 18 15, 11, 13 11, 11, 14 11, Page 9, 12 9, 7, 11 7, 7, 11 7, 7, 11 7, 9 7, Other Species p. 14;CellSignaling pp. 15-19. 15 15 10 16 16 10 16 16 16 11 17 17 17 17 17 14 8 7 7 7 8 9 9 9 9 9 8 7 9 8 8 8 7 7 8 Myostatin/GDF8 ...... 3(MYL3) Chain Light Myosin (MIA) Protein Activity Inhibitory Melanoma Melatonin MMP-8 MR-ProADM MPIF/CCL23 MMP-9 mTOR (total) (Ser2448) mTOR (Ser212) MSK1 (autoantibody) (MPO)Myeloperoxidase Mucin-1 (MUC1) (autoantibody) Myoglobin MEK1 (Ser222)MEK1 Oxytocin (OPG)Osteoprotegerin MMP-12 MMP-10 MMP-1 MIP-4/PARC/CCL18 MIP-3β /CCL19 /CCL20 MIP-3α MIP-2/CXCL2 MMP-13 MMP-3 MMP-2 MMP-7 MIF Midkine MIP-1δ/MIP-5/CCL15 /CCL4 MIP-1β /CCL3 MIP-1α MIG/CXCL9 Osteopontin (OPN)Osteopontin Osteonectin/SPARC (OSTN)/Musclin Osteocrin Mi-2 (autoantibody) Mesothelin p53 (autoantibody) (total)p38/SAPK2A/B ...... (Thr180/Tyr182) p38/SAPK2A/B (total) p21 P16-INK4a (autoantibody) P (OC)Osteocalcin Osteoactivin (OSM) Oncostatin-M O NAP-2/CXCL7 N NCAML1/L1CAM/CD171 Neuron-specific Enolase Neuron-specific (NSE) (NRGN) Neurogranin NFκB (total) NFκB (Ser536) NFκB Neutrophil Elastase-2 (ELA2) Neurotensin NGAL/Lipocalin-2 ...... proBNP NT (interaction) NOXA/Mcl-1 Notch1 NGF Analyte ......

...... 8, 9, 10, 13, 14 7, 8, 11, 12, 13 8, 10, 12, 13 9, 10, 12, 14 9, 10, 13, 14 ...... 15, 18, 19 18, 15, 15, 18, 19 18, 15, 16, 18, 19 16, 18, 19 10, 13, 14 10, 13, 14

8, 12, 13 12, 8, 8, 12, 13 12, 8, 8, 9, 12 7, 8, 11 10, 13 10, 11, 13 11, 16, 17 16, Page 8, 12 8, 8, 12 8, 8, 12 8, 8, 12 8, 9, 10 9, 7, 10 7, 7, 11 7, 7, 11 7, 7, 11 7, 8, 9 8, 13 10 16 10 16 10 10 10 10 10 16 16 16 9 8 7 8 9 8 7 8 8 8 8 9 9 9 7 8 9 9 8 MILLIPLEX® map ANALYTE INDEX FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.

MILLIPLEX® map 9 9 8 9 8 8 8 7 9 9 7 17 17 17 17 17 16 16 10 10 16 16 16 10 10 10 10 10 10 10 10 15 15 13 7, 9 7, 11 7, 11 7, 10 9, 12 9,13 Page 10, 14 10, 12 10, 12 7, 9, 11 9, 7, 9, 12, 13 12, 9, 13 12, 9, ...... 9, 11, 12, 13, 14 14 13, 12, 11, 9, 9, 11, 12, 13, 14 13, 12, 11, 9, 14 13, 12, 11, 9, 8, 10, 11, 13, 14 13, 11, 10, 8, 9, 11, 12, 13, 14 13, 12, 11, 9, 8, 11, 12, 13, 14 13, 12, 11, 8, 14 13, 12, 11, 8, 14 13, 12, 11, 8, ...... 7, 8, 9, 11, 12, 13, 14 13, 12, 11, 9, 8, 7, 7, 9, 10, 11, 12, 13, 14 13, 12, 11, 10, 9, 7, ...... MerckMillipore.com/milliplex | 25 MerckMillipore.com/milliplex ...... Analyte Tenascin-C (TN-C) Tenascin-C Testosterone TFF-3 TGF TGFβ1 TIMP-1 TIMP-2 TIE2 (panTyr) TIE1 (panTyr) TIM-3 Syndecan 4 (SYND4)...... Syk (panTyr) Tissue Factor (TF)...... TLR-2 Tau (Total) Tau (Thr181) Tau TIMP-3 TIMP-4 TGFβ2 TGFβ3 (total) TGFβRII Thrombomodulin (TSP-1) Thrombospondin-1 (TSP-2) Thrombospondin-2 TNFR1 (total) TNFα TNFβ/Lymphotoxin-α (LTA) TPO TRAIL/TNFSF10 Y Yes (Tyr421) Z Z AP-70 (panTyr) U ucMGP Uromodulin V VEGF VEGF-A YKL40/CHI3L1 T T3 TARC/CCL17 VEGF-C T4 VEGF-D (panTyr) VEGFR1/Flt-1 (panTyr) VEGFR2/KDR/Flk-1 (panTyr) VEGFR3/Flt-4 Vitamin binding D protein Transglutaminase 2 (TGM2) 2 Transglutaminase TRAP5 TREM2 Troponin I (TnI) von Willebrand Factor (vWF) Factor Willebrand von Troponin T (TnT) TSC2 (total) TSC2 (Ser939) TSH TSLP TWEAK EMDMILLIPORE.COM/MILLIPLEX | 25 EMDMILLIPORE.COM/MILLIPLEX 9 8 8 9 9 8 9 9 8 8 17 17 17 17 17 17 19 10 10 10 10 16 16 16 10 10 10 10 10 10 10 18 9, 12 8, 10 8, 12 8, 12 8, 12 8, 12 8, 12 8, 12 8, 12 Page 17, 18 10, 14 16, 17 7, 9, 14 9, 7, 8, 9, 10 9, 8,

7, 11, 12 11, 7, 7, 12, 14 12, 7, 9, 12, 14 12, 9, 9, 10, 12 10, 9, 8, 10, 12 10, 8, 8, 12, 17 16, 17, 19 17, 16, 10, 13, 14 13, 10, ...... 9, 10, 12, 13 12, 10, 9, 8, 10, 12, 16 12, 10, 8, 16 12, 10, 8, 16 12, 10, 8, 7, 8, 9, 11, 14 14 11, 9, 8, 7, 7, 8, 9, 11, 12 12 11, 9, 8, 7, 8, 9, 10, 12, 13 12, 10, 9, 8, ...... Analyte Sm (autoantibody) Sm sIL-1RII sIL-2Rα sIL-4R sIL-6R sIL-6Rα sL-Selectin sIL-1RI SDH sHer3/sEGFR3/sErbB3 sHGFR/sc-Met sICAM-1 sHer2/sEGFR2/sErbB2 Scl-70 (autoantibody) Scl-70 SDF-1/CXCL12 sGP130 sHER1/sEGFR/sErbB1 Soluble Superoxide Dismutase 2 (sSOD2) (autoantibody) SOX2 Sclerostin (SOST) Sclerostin SMAD2 (Ser465/467) SMAD2 SMAD3 (Ser423/425) SMAD4 (total) sNCAM sVCAM-1 sNeuropilin-1 sP-Selectin (autoantibody) Survivin/BIRC5 Serum Amyloid P (SAP) sRAGE sE-Selectin Serum Amyloid A (SAA)...... Globulin Binding Hormone Sex (SHBG) (sFasL) Ligand sFas Src (Tyr419) sFas/TNFRSF6 Soluble Superoxide Dismutase 1 (sSOD1) sST2/IL1RL1 STAT1 (total) ...... (Tyr690) STAT2 STAT3 (total) S TAT3 (Ser727) SCF S TAT1 (Tyr701) SSA/Ro52 (Sjögren’s Syndrome- (Sjögren’s SSA/Ro52 related antigen A/Ro52 kDa) (autoantibody) Syndrome- (Sjögren’s SSA/Ro60 related antigen A/Ro60 kDa) (autoantibody) Syndrome-related (Sjögren’s SSB/La (autoantibody) antigen B/La) sVEGFR2/sKDR/sFlk-1 sVEGFR3/sFlt-4 sTNF RI (sTNFR1) sTNF sTNF RII (sTNFR2) Substance P suPAR sVEGFR1/sFlt-1 sTie-2 sCD40L STAT3 (total) S TAT3 (Tyr705) STAT5A/B...... (Tyr694/699) STAT6 (Tyr641) STAT5A/B (total) STAT5A/B 9 9 8 8 9 8 9 8 9 8 8 9 9 9 9 9 8 8 9 9 14 16 10 16 10 16 10 16 10 10 10 10 10 15 15 15 15 12 12 12 7, 11 7, 11 7, 10 7, 10 9, 10 8, 12 8, 12 Page 10, 14 15, 16 15, 16 10, 13 9, 10, 14 10, 9, 9, 10, 12 10, 9, 9, 12, 13 12, 9, Analyte map (continued) Index ® 8, 10, 12, 13 12, 10, 8, ......

...... 7, 10, 11, 13, 14 13, 11, 10, 7, 9, 11, 12, 13, 14 14 13, 12, 11, 9, 8, 11, 12, 13, 14 14 13, 12, 11, 8, 8, 11, 12, 13, 14 14 13, 12, 11, 8, ...... Analyte sCD31/sPECAM-1 sCD137/4-1BB/ TNFRSF9sCD14 sCD30 sCD163 sc-Kit/SCFR sAXL p53(Ser15) (Ser46) p53 RPS6 (total) RPS6 (Ser235/Ser236) RNP/Smith (RNP/Sm) (RNP/Sm) RNP/Smith (autoantibody) sALK-1 S S1B RANTES/CCL5 Ribosomal (autoantibody) P (Ribonucleoprotein) RNP (autoantibody) RBP4 Renin Resistin R RANKL MILLIPLEX Human pp. 7-10; Primate p. 11; Mouse pp. 11-13; Rat pp. 13-14; 11-13; Rat pp. 11; Mouse pp. 7-10; Primate p. Human pp. 15-19. pp. 14; Cell Signaling Other Species p. PD-L1 p70S6 Kinasep70S6 (total)...... Kinase (Thr389/412) p70S6 PAI-1 (total) Pancreatic Polypeptide (PP) ...... PARK5/UCHL1 (Parkinson’sPARK7/DJ1 Disease Protein 7)...... PCNA (Proliferating Cell Nuclear (autoantibody) Antigen A) PD-1 PDGF-AA PDGF-AB/BB PDGF-BB PDGFRα (panTyr) PDGFRβ (panTyr) Proteinase 3 (autoantibody) 3 Proteinase (free) PSA PSA (total) PTEN (total) PTEN (Ser380) PTGDS PTH PYY (total) Progesterone Prolactin Properdin/Factor P Placental Growth Factor...... 2 (PLGF-2) Placental Growth Factor (PLGF)...... Platelet Factor (PF4)/CXCL4...... (autoantibody) 1 PM/Sci Protein) PRNP (Prion PLA2G7 PL-12 (Alanyl-tRNA Synthetase)PL-12 (autoantibody) PEDF (PTX3) Pentraxin-3 Perforin Periostin Progranulin (pGRN) ProMMP-9 FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. LUMINEX® SYSTEMS

LUMINEX® 26 |MerckMillipore.com/luminex provide you themaximum powerofLuminex available, ofmultiplexanalytes portfolio we instrument solutions. Combinedwiththelargest xMAP Luminex Enhance your generationwithourLuminex data Driving AssayConsistency Luminex Multiplexed Biomarker Analysis using Specifications • • • results, whichinstrumentascientist usesfortheirresearch. nomatter tofitthecapabilitiesof each platform. This software adapts used platformforbiomarker screening and All systems run ontheLuminex Optics Cat. No.Cat. Read Time(MicrotiterPlate) Instrument Software Multiplex Capacity Bead Compatibility Hardware Description (W xDH) Dimensions includingPC Dynamic Range Applications LIS Security Automation CFR 21Part 11 Weight (Analyzer) including high-throughputwashingoptions. andfasterwashingtechniques,enabling better toamagneticfield, rapidly andefficiently Magnetic bead-basedformat:Responds sample usingamaximumof25µLperwell. time, screen more inasingle than40analytes Low samplevolume: Withminimalhands-on multiple parameters. toexplore sets user toconstructcleanerdata is lesspronetousererror, enablingtheend- generatedsimultaneously Multiplexing: Data ® technology, themosttrusted,widely ® xMAP ® technology offers many advantagescompared tootherimmunoassay technologyoffers methods: ® Systems ® xPONENT Magnetic andnonmagnetic The fastest,mostflexible ~75 min/384-wellplate FLEXMAP 3D ~20 min/96-wellplate Flow Cytometry-based Protein/Nucleic Acid Lasers/APDs/PMTs 110 x6263cm 77.1 kg (170lbs) xPONENT ® 4.2 data acquisition platformproviding 4.2data aconsistentexperience, inputto fromdata platform. 4.5 logs 40-014 500 3 3 3 3 ® ® System 4.2 ®

®

used Luminex quickly andreliably. technology enableyou togainmore information and industry-leading experiencewithmultiplex accessories andsoftware. Ourongoingdedication a preferred distributorofLuminex protein analysis. AsaLuminex • • Gen 2oftheoriginalwidely Magnetic andnonmagnetic Luminex comprehensive socriticaltoyour data work. do your work faster, gainingtheearlyand 96- or384-wellformat,enablingyou to of different inasinglesample, analytes in measure theconcentrationofalargenumber Speed/high-throughput: Simultaneously cannot provide. thatsolid-phasedflatarraysstandardization of xMAP Reproducibility: High-volume production ~40 min/96-wellplate Flow Cytometry-based 100 (80forMagPlex Protein/Nucleic Acid Lasers/APDs/PMTs 115 x6050cm 49 kg (113lbs) xPONENT 3.5 logs ® 40-012 200™System ® ® 3 3 3 3 100™System. microspheres allowsassay ® 4.2 ® ) effective modelforrunning The newestmostcost- ~60 min/96-wellplate Protein/Nucleic Acid Fluorescent Imager MAGPIX 17.5 kg (38.5lbs) ® LED/CCD Camera 70 x6050cm protein assays. partner, weare xPONENT ® Magnetic 3.5 logs 40-072 instruments, 50 3 3 3 3 ® System ® 4.2 LUMINEX® SERVICE / CONSUMABLES / TRAINING FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.

LUMINEX® ® 40-285 Cat. No. Cat. No. Cat. No. 40-50015 40-50018 SVCLUMPLT MPXDF-4PK SVCLUMSLV SVCLUMBRZ Protocol No. SVCLUMGLD VP-FM3D-4.2 Coming Soon! F3D-CAL-K25 MPX-CAL-K25 LX2R-CAL-K25 VP-MAGPIX-4.2 F3D-PVER-K25 MPX-PVER-K25 SVCLUMPLT360 LX2R-PVER-K25 SVCLUMGLD360 92-00040-00-002 SVCLUMPLTFM3D SVCLUMSLVFM3D SVCLUMBRZFM3D SVCLUMGLDFM3D SVCMAGPIXPLATPM SVCLUMGLDMAGPIX SVCMAGPIXGOLDPM SVCLUMPLT360FM3D SVCLUMGLD360FM3D

® 1 L ® MerckMillipore.com/luminex | 27 MerckMillipore.com/luminex 20 L • • • • • 1 each 200™ and required for FLEXMAP 3D 200™ and required 25 uses 25 uses 25 uses 25 uses 25 uses 25 uses 2 (PM) ® 4.2 platform Pack Size Pack ® for xPONENT ® Systems Installation Systems Qualification 4 pack, 750 mL ea ® 200™ xPONENT xPONENT • • • • • • • • • ® ® 1 (PM) FLEXMAP 3D IQ/OQ Protocol 4.2 platform Luminex Field Service** 4.2 platform MAGPIX only ** Optional for Luminex Luminex (IQ) and Operational Qualification (OQ) ® instrument. Our staff can help you install Our staffinstrument. the help can and platform, to use your train you instrument, appropriate with the to supply you continue run it as required. to reagents

Repair ) ® Unlimited Emergency Systems Systems ® Cat. No. Cat. No.

® TRONSITE-MA • • • • • • • • • • • • • • • TRONSITE-MPX instruments, instruments, ) TRONSITE-FM3D ® TRONSITE-LX200 ® Remote Remote Support TRONSITE-BIOTEK TRONSITE-MAGPIX Unlimited response Onsite 2-business days 100/200™ & FLEXMAP 3D ® Calibration Kit Kit Verification Performance , Gold , Gold 360* , Platinum , Platinum 360* , Bronze , Silver System Onsite System ® ® ® ® ® ® ® ® ® Analyst 5.1 Software 5.1 Software Analyst map Kits Training ® ® System System 200™ Calibration Kit (xPONENT Kit (xPONENT Verification 200™ Performance 200™, Gold 200™, Gold 360* 200™, Platinum 200™, Platinum 360* 200™, Bronze 200™, Silver 200™ System Onsite 200™ System Drive Fluid Drive Calibration Kit Kit Verification Performance , Platinum , Standard , Gold System Onsite System ® ® ® ® ® ® ® ® ® ® ® ® ® ® ® ® ® Washer Onsite Installation Washer ® 1 Year Warranty Plans Warranty 1 Year Luminex Luminex Luminex MAGPIX FLEXMAP 3D FLEXMAP 3D FLEXMAP 3D Description MAGPIX Description Luminex Luminex Installation and Training and Training MILLIPLEX Luminex FLEXMAP 3D Installation and Training Onsite Installation and Training BioTek FLEXMAP 3D Installation and Training MILLIPLEX Luminex FLEXMAP 3D FLEXMAP 3D MAGPIX Luminex Luminex Sheath Fluid (20x concentrate) FLEXMAP 3D MAGPIX MAGPIX Sheath Fluid for Luminex MAGPIX Luminex FLEXMAP 3D Handheld Magnetic Seperator Block MAGPIX Onsite 1-business day response response Onsite 1-business day 1 (PM) = includes 1 preventive maintenance 2 (PM) = includes 2 preventive maintenances 2 preventive maintenance 2 (PM) = includes 1 (PM) = includes 1 preventive Luminex by developed * 360 service includes on-site support for assays Training Consumables Service Contracts As a preferred partner of Luminex As a preferred Instrument and Kit Service,and Kit Instrument Consumables Training and engineers tailored to the use of your new engineers of your tailored to the use we supply full servicewe supply of sale and at point service Multi-level research. your throughout from Luminex cover contracts provide FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. MILLIPLEX® ANALYST

ANALYST 28 |MerckMillipore.com/luminex for MILLIPLEX In developing thecurve-fitting algorithms packages. missed by analysis existingmultiplexdata andareregions commonly canbeimportant curves.and highendsofstandard inthese Data deriveddesigned tofocusondata fromthelow MILLIPLEX using real-life sets data from ourexperienceinassay manufacturingandvalidated algorithm,developed Use ouradvanced curve-fitting MILLIPLEX minimum detectable concentration. minimum detectable Table fromHSTCMAG-28SK curve performance 1.Standard IL-6 analytes andTNFα using MILLIPLEX Std 7 Std 6 Std 5 Std 4 Std 3 Std 2 Std 1 Blank TNFα Std 7 Std 6 Std 5 Std 4 Std 3 MILLIPLEX Description Std 2 Std 1 Blank IL-6 Standard Curves generatedusing5parameterlogarithmiccurve fitusingMILLIPLEX Figure 6.Example fromtheMILLIPLEX data Easily export complete multiplex data foruseinpresentations andrecord completemultiplexdata keeping.Easily export MILLIPLEX DC =(0.047,2025) =0.16%,R²0.9999 Chi =1.88%,CV 5-P.log(2.96,Curve statistics: 11.89,0.52,3.19,0.31) ® Analyst 5.1 “Flex” Software Analyst 5.1“Flex” ® Analyst 5.1 “Flex” software Analyst was 5.1“Flex” ® Analyst 5.1 “Flex” Software, Analyst 5.1“Flex” ® Analyst Reports 5.1Detailed Exp pg/mL 109.38 ® 437.5 27.34 187.5 46.88 11.72 1750 6.84 1.71 0.43 2.93 0.73 0.18 750 Analyst 5.1 “Flex” Software Analyst 5.1“Flex” 0 0 ® Human High Sensitivity TCellpanel(HSTCMAG-28SK HumanHighSensitivity IL-6, analytes andTNFα). 23262 10648 18348 246.5 954.5 22.25 211.5 3665 8262 3052 83.5 38.5 MFI 847 36 75 24 DC =(0.097,7853) =0.14%,R²0.9999 Chi =1.66%,CV 5-P.log(2.13,Curve statistics: 11.10,0.41,3.06,0.47) Obs pg/mL 428.21 111.88 759.68 181.59 MILLIPLEX of lowquality. dongle-activated Withits format, and thatworkwelleven curves with standard CVs atthelowandhighends ofthecurves, determine thefitthatwouldgive thelowest curves to standard using actualexperimental simulations weresets runon600+data plug andplay functionality. used fromthelabtoofficewiththissimple 27.45 11.99 1758 6.66 1.74 0.43 47.5 2.86 0.74 0.18 — — ® Analyst 5.1. ® Analyst 5.1 “Flex” Software Analyst may 5.1“Flex” be % CV 11.12 11.31 11.79 1.01 0.05 3.43 3.73 4.96 2.54 3.93 1.92 5.02 4.73 5.68 9.18 5.51 % Recovery expected concentrations. observed concentrationswith between ofthedistance statistic Chi –TheChi-Squared test each dilutionlevel. curve replicatesof standard at –TheCoefficientofCV Variation range. concentration DC –Thedetectable Notes: ® Analyst 5.1.minDC= 100.44 102.29 100.38 101.83 101.29 101.33 102.34 100.69 98.11 97.42 99.62 96.85 97.64 99.99

— — minDC pg/mL

Cat. No.Cat. 40-086 0.097 0.047 WASHING & DISPENSING FOR MILLIPLEX® map ASSAYS FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.

BIOTEK® 40-099 Cat. No. -approved ® xMAP ® map 384-well ® MerckMillipore.com/biotek | 29 MerckMillipore.com/biotek map and Luminex ® washer advantages: washer advantages: ® Fast and hands-free full plate washing and hands-free Fast MILLIPLEX High-energy neodymium iron boron magnetsHigh-energy neodymium with for rapid separation of multiplex beads superior retention a state-of-the-art, 405™ TS models have backlit touch screen LED high-resolution and flexible user interface for an intuitive instrument 405™ the built-in TS models come with cleaning enabling easy Advantage™ Ultrasonic with the toughest of sample types even BioTek • • • • • the 405™ TS model offering an easy-to-use and and an easy-to-use TS model offering the 405™ looking for a those For screen. glove-touchable MultiFlo™ format, the assay automated more FX will allow users in a to dispense kit reagents excellent fashion, to ensure timely and uniform reproducibility. assay MultiFlo™ FX Automated MultiFlo™ ® MultiFlo™ FX Automated Reagent Dispenser FX Automated Reagent MultiFlo™ ® Fully automated process: load standards, QCs and process: load standards, automated Fully samples and walk away plates Optimized for both 384- and 96-well The performance of a larger robot for a fraction of the cost programmable washer enables precise Self-contained, complete control while washing fluidic delivery—ensuring plates 96-well and 384-well assay Description BioTek BioTek The perfect instrument for MILLIPLEX • • • • Reagent Dispenser Reagent Assays , we offer the the , we offer ® Assays map ® Washing & Dispensing Solutions for for Solutions & Dispensing Washing 405™ Select TS (touch screen) Washer 405™ Select TS (touch screen) ® ® BioTek MILLIPLEX In partnership with BioTek BioTek latest advancements in 96- and 384-well plate and 384-well plate in 96- latest advancements designed system a fully automated washing: plate through entire to quickly wash an washing and vacuum biomagnetic separation, systems which allow We offer two filtration. filtration options—with magnetic and vacuum FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. SMC™ (SINGLE MOLECULE COUNTING) TECHNOLOGY

SMC™ 30 |MerckMillipore.com/smctech background +increased signal (SMC™)Technology:Single MoleculeCounting Reduced SMC The SMCxPRO™ andErenna countingimprovesDigital anddynamicrange sensitivity extending thedynamicmeasuringrangebeyondextending whatcanbeachieved withtraditionaltechnologies. curve,across aspectrumoftimeseriesforsinglestandard thus improving assay and sensitivity curve.additional standard TheSMCxPRO™ event instrumentdigital events algorithmcomputesdigital sumofallphotonsrecorded algorithmcomputesthetotal as an ErennaR instrument, aproprietary background threshold. Thismodel canhave limiteddynamic range, so athigherconcentrationsonthe SMC™ technologyachieves improved signal- counting, elution stepandrobustdigital as shownbelow. By combiningauniqueassay workflow similartotraditionalELISAtechnology, immunoassay whilefollowinga performance SMC™ technologyprovides maximum each biomarker into Antibodies translate 12 So ™ nal Steps 2-3 lution t a signal Technology e of n te rest

in

ST AND ® lat Ca instruments capture events instruments thesumofalldigital countedabove a ptur ARD e or e

e on ad

dissociate fromthe labeled detection Fluorescent dye- immunocomplex 3 antibodies ete Step 4 ct system. and highlevels ofexpression ononecomplete provides enhancedquantificationatbothlow technologies. TheSMC™technologythus to-noise ratiosover traditional immunoassay 45 br Co lut ok mple en e

apar is ch t PR emic all OPRIET

individual molecules On theSMCxPRO™ laser, CCDcamera instrument, signal is collectedfrom by anAvalanche using a rotating using arotating fluorochromes and counting free-floating Photodiode AR Step 5 dete Sa uanti mple Y ct io

f is n

ta

dete gs

ct co ed unte b d la se r

SMC™ (SINGLE MOLECULE COUNTING) TECHNOLOGY FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.

SMC™ Cat.No. 03-0109-02 03-0156-00 03-0159-00 03-0164-00 03-0059-01 03-0112-00 03-0157-00 03-0118-00 03-0088-00 03-0163-00 03-0028-00 03-0160-00 03-0052-00 03-0161-00 03-0089-01 03-0155-00 03-0145-00 03-0146-00 03-0092-00 03-0154-00 03-0153-00 03-0100-01 03-0051-00 03-0053-00 03-0094-00 03-0058-00 03-0102-00 03-0119-00 03-0108-00 03-0047-00 03-0049-00 03-0099-01 03-0055-00 03-0103-00 03-0014-07 03-0068-00 03-0067-00 03-0072-00 03-0056-00 03-0024-03 03-0057-00 03-0025-06 STOCK STOCK STOCK STOCK STOCK STOCK STOCK MTO STOCK STOCK MTO or MTO STOCK STOCK STOCK STOCK STOCK STOCK STOCK STOCK STOCK STOCK STOCK STOCK MTO MTO STOCK MTO MTO STOCK STOCK MTO MTO STOCK MTO MTO STOCK MTO STOCK MTO STOCK STOCK MTO STOCK MTO instruments, instruments, ® 4 MerckMillipore.com/smctech | 31 MerckMillipore.com/smctech Sample Type P, S P, S P, S P, S P P, S P, S U S, P, P P, S P P, S P P, S P, S P, S C, P C, P P, S P, S P L P, S P P, S P, S P, S P, S S P P L P, S P, S S P P P P P P, S P 3 H H H H H H H H H H H H H H H H H, M, R H, M, R R, C, GP H, Cy, R, C, GP H, Cy, H, M, R Species H, M, R H H H H H H M H H H, M, R H H H H H H H H, M, R, C H H, M, R, C and SMCxPRO™. 2 ® Median Endogenous (pg/mL) P: 0.65; S: 0.46 147 P: 65; S: 75; U: 2.3 P: 0.85; S: 0.78 0.21 P: 0.39; S: 0.33 P: 0.31; S: 0.30 P: 1.1; S: 1.2 3.3 0.18 P: 0.45; S: 1.08 P: 7.4; S: 0.12 0.12 P: 0.18; S: 0.18 1.3 1.3 P: 152; CSF: 1366 P: 152; CSF: 191 P: 15; CSF: 1.75 1.75 44 NA 3.38 0.86 2.75 38.6 0.21 4.52 4.91 17 0.79 NA 3.6 0.12 0.53 66.5 0.2 1.06 1.01 3.46 0.13 17.8 1 0.1 3.91 0.2 0.2 0.04 0.04 0.07 0.2 0.2 0.1 0.2 0.2 0.04 0.04 0.08 0.08 5.86 0.98 0.69 0.69 0.781 LLOQ LLOQ (pg/mL) 0.98 0.1 0.2 1.2 0.4 0.2 3.91 0.39 0.08 0.2 7.8 0.24 0.03 0.2 0.2 0.02 0.39 0.39 0.4 0.05 0.39 NEW

6,7 6,7 Immunoassay Kits Immunoassay

NEW NEW ™ 6,7 NEW NEW NEW NEW

6

NEW NEW NEW NEW

NEW 6,7

6,7

5

6 6,7 6 6,7 6 6,7 6 6,7

6 6,7 6 6,7 6 6,7 but not optimized for peak performance. been tested, Optimized for first species type listed. Other listed species have C=Canine Monkey; H=Human; M=Mouse; R=Rat; GP=Guinea Pig; Cy=Cynomolgus Key: Fluid (CSF) U=Urine; C=Cerebrospinal P=Plasma; S=Serum; L=Lysate; Optimized for use in sample type(s) listed. Key: Analyte Akt1 (Ser473) IL-13 IL-15 v2 IL-17F Heterodimer v2 IL-17A/F IL-23 IL-23 KIM-1 TNFα TNFα TNFα IL-1β IL-2 IL-4 IL-5 IL-6 IL-7 Amyloid beta 1-42 Amyloid cTnI cTnI G-CSF IFN-γ Akt1 (total) IL-4 IL-6 / CXCL8 IL-8 v2 IL-17A IL-17F IL-21 VEGF GM-CSF IL-1α Amyloid beta 1-40 Amyloid IL-10 IL-17A IL-22 GLP-1 (active) GLP-1 IL-1β IL-12 GLP-1 (total) GLP-1 IL-13 v2 IL-13 Glucagon Assay characteristic data is representative for both Erenna characteristic data is representative Assay to the new kits section. list of changes refer format kit. For New streamlined Median Endogenous: Minimum of 10 samples from individual donors assessed for ability to quantify baseline biomarker values. Median Endogenous: Minimum of 10 samples from individual donors assessed for ability to quantify baseline biomarker   Plate-based assay. LLOQ: Lowest point on standard with CV curve < 20% and accuracy within 20% of expected values. LLOQ: 6 7 and Erenna list of kits a complete qualified on both the SMCxPRO™ For 2 3 4 5 1 SMC visit MerckMillipore.com/smcxpro visit MerckMillipore.com/smcxpro FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. SMC™ (SINGLE MOLECULE COUNTING) ASSAYS

SMC™ 32 |MerckMillipore.com/smctech Erenna Discovery immunoassays are provided torunonyour ascompletekits • • verifiedused toqualify immunoassays A comprehensive setofcriteriaevaluating are ultimatequantitative performance Validation CriteriaforVerified Immunoassays • • • Immunoassay Kits Mouse Plate-basedDiscovery Analyte Mouse TNFα Mouse IL-22 Mouse IL-21 Mouse IL-17F Mouse IL-17A Mouse IL-13 Mouse IL-10 Mouse IL-5 Mouse IL-4 across experiments Spiked andun-spiked sampleswithin20% run onmultipleplatesover multiple days. Inter- andintra-assay precision: Samples within 20%ofexpectedvalues <20%andaccuracy curve withCV standard Lower Lowestpointon limitofquantitation: Minimal samplevolume Simple workflow LLOQ Reproducible ® platform (pg/mL) LLOQ 0.49 0.49 0.98 3.91 0.24 0.49 100 4.9 6.2 Median Endogenous (pg/mL) 28.4 44.0 12.0 195 2.2 9.2 8.3 6.1 NA • • • quantify biomarkerquantify levels to assessedforability from individualdonors Endogenous range:Minimumof10samples expected value AssaysDilutional linearity: within20%of 80–120% spiked recovery withacceptable between Spike recovery: Minimumof10samples Species M M M M M M M M M Sample Type S S S S S S S S S 03-0137-00 03-0127-00 03-0126-00 03-0125-01 03-0123-00 03-0133-00 03-0134-00 03-0132-00 03-0136-00 Cat. No.Cat. SMC™ (SINGLE MOLECULE COUNTING) CUSTOM ASSAYS AND SERVICES FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.

CUSTOM MerckMillipore.com/smctech | 33 MerckMillipore.com/smctech Reagent sourcing Reagent and selection Screening Antibody derivatization Prototype sample testing Proprietary kit sample testing kit testing SMC™ immunoassay SMCxPlore Conversion Assay to SMC™: antibody pairs, 600 proven With access to over currently that isn’t a SMC™ assay let us develop available. Reagent Selection Reagent • • • Sample Testing • • • Pharmacodynamics Immunogenicity Pharmacokinetics Immunoassay development Immunoassay requirements to sponsor verification Biomarker Preclinical to early clinical sample testing Preclinical We are happy to announce the launch of our new SMCxPlore Services, customized streamlined customized streamlined Services, launch of our new SMCxPlore to announce the happy are We or critical samples. analyte of interest technology with your SMC™ options to “explore” Sample testing of up to 30 of your critical Sample testing of up to 30 of your SMC™ samples using one of our highly validated immunoassays. SMCxPlore of Concept Proof Sample Testing: Development of a prototype assay using your using your of a prototype assay Development for bringing antibody pairs and standard. Ideal in house. assay your SMCxPlore Assay Development SMCxPlore Development Assay Feasibility: • • • • • Biotherapeutic Development • Our services include: Discovery Biomarker Collaborative & Development SMC™ Technology SMC™ for your own assay your to develop the time or resources have always understand don’t you We years 130 over scientific team has Our custom samples. critical or to test your interest protein of us help you Let expertise in SMC™ technology. with core experience development of immunoassay a custom designed program into clinical trials with research from discovery program accelerate your for you. Expert Custom Assays & Sample Testing for for Expert Testing Sample & Assays Custom FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. SMC™ (SINGLE MOLECULE COUNTING)

SMCxPRO™ 34 |MerckMillipore.com/smcxpro • • SMCxPRO 2 1 Min. InstrumentPerformance Specifications • • • • Instrument Specifications • • Assay Format • Read PlateFormat • RequirementNetwork/PC Response measurements from30fMcalibrator, n=20  Dynamic Range Precision Limit ofDetection(LoD) Background Slope Metric with fluorophore Determined fromcalibratorset:0-300 fMof150kDantibodylabeled included (32-or64-bitcompliant) xPRO OperatingandAnalysis Software server IPaddressA static andanFTP (Operating range180-250V) International: 230VAC, 50-60Hz (Operating range90-125V) US: 115VAC, 50-60Hz Weight: 22.7kg (50lbs) mm Wx444.5D(16"H14"W17.5"D) Instrument dimensions:406.4mmHx355.6 Plate-based assays Bead-based assays 384-well plate Microsoft Windows ™ ® 7/10OperatingSystems Immunoassay System Specifications >1.2 response/fM <3 response/fM Specification <10% CV Jitterbug PlateShakerJitterbug Tecan Hydroflex Microplate8-Channel Washer BioTek SMCxPRO™ PQService SMCxPRO™ IQ/OQInstallation SMC™ Advanced Training –3days SMCxPRO™ System Training – 1 day SMCxPRO™Standard Installation SMCxPRO™ CompleteSystem SMCxPRO™ ProductServices &Ancillary >4 logs <1 fM ® 2 1 1 405Touch MicroplateWasher withMAG plate for more information. Installation andTrainingInstallation Erenna immunoassays foruse ontheSMCxPRO™ and provide thetoolsyou needtodevelop your own our globaltrainingcenters. Thistrainingwill hands-on trainingatyour oratoneof facility bead- orplate-basedassay format.We offer application-specific immunoassays ineither (SMC™) technologywhiledeveloping your own Take advantage ofultrasensitive digital Advanced CustomerTraining • • • forSMCxPRO™ Platform Support Three Levels ofOnsiteService • • • SMC™ Assay Development Training day -extra SMC™ Assay Development Training -3 days Description maintenance visitforoneyear LaborforonepreventativeEssential Service: floating visitforone year for onepreventative maintenanceandone Labor,Advanced Service: travel andexpenses for oneyear travel, forany service expensesandallparts Total includinglabor, Unlimitedvisits Service: availableOptional IQ/OQandPQServices performance Run ofASSIST programtoverify instrument calibration ofunit Set upofunitonbenchandcomplete ® instruments. Askyour salesspecialist 95-0004-05 70-0009-00 95-0014-00 96-2013-00 96-2012-00 96-2023-00 96-2022-00 96-2011-00 95-0100-00 Cat. No.Cat. 96-2025-00 96-2024-00 Cat. No.Cat. SMC™ (SINGLE MOLECULE COUNTING) ANCILLARY EQUIPMENT FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.

SMC™ Cat. No. Cat. No. Cat. No. 70-0009-00 95-0014-00 95-0004-05 Assays ™ , we offer the latest , we offer the latest ® MerckMillipore.com/smctech | 35 MerckMillipore.com/smctech 405™ TS Magnetic 96-well 405™ TS Magnetic 96-well HydroFlex™ 8 Channel Plate HydroFlex™ ® ® Boekel Jitterbug Heated Microplate Shaker Jitterbug Heated Microplate Shaker Boekel (115V) Description Tecan Description Description BioTek Washer Washer Complete with Touch Screen and Screen Complete with Touch Washer Ultrasonic Cleaning This heated microplate shaker is ideal for Immuno- This heated microplate shaker shaking of heating and SMC™ requiring like assays risk of Includes inner lid to reduce microplates. contamination. microplate washer is a flexible The HydroFlex™ washer with automated microplate strip washing, filtration in magnetic bead washing and vacuum Along with microplate shaking 96-well plate formats. system and automated buffer switching included, the comes specifically optimized for use with SMC™ designed with the inclusion of a specially assays magnetic plate and customized pre-programmed plate washing protocols. In partnership with BioTek advancements in 384-well plate washing: a fully in 384-well advancements designed to quickly wash an automated system washing plate through biomagnetic separation, entire comes specifically system filtration. The and vacuum with the SMC™ assays optimized for use with designed magnetic plate and inclusion of a specially washing proto- plate customized pre-programmed and offers The 405™ TS model an easy-to-use cols. screen. touchable glove 405TS 384 Well Plate Washer Plate 384 Well 405TS HydroFlex™ 8 Channel Plate Washer HydroFlex™ ® ® BioTek Tecan Boekel Scientific Jitterbug, 130000, Heated Microplate Shaker (115V) Scientific Jitterbug, 130000, Heated Microplate Shaker Boekel Ancillary Equipment to Optimize your SMC Ancillary your Optimize to Equipment FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. ELISAs

ELISAs 36 |MerckMillipore.com/elisa_ria ∆ •• * development. investigating ordefiningoutcomeswithintoxicological diseasestates studiesduringdrug metabolic aconsistentandreliable andendocrineELISAsallowsend-users methodof Our menuofmetabolic ELISAs Metabolic/Endocrine Millipore BrandELISAs Glucagon ∆ GLP-2 GLP-1 (total) (active) ∆•• GLP-1 HighSensitivity GLP-1 (active) GIP (total) GIP (total) GIP (total) Ghrelin (total) Ghrelin (total) Ghrelin (active) Ghrelin (active) FGF-23 FGF-21 FGF-21 C-Reactive Protein(CRP) C-Reactive Protein(CRP) C-Peptide 2 C-Peptide C-Peptide Leptin “DualRange” Amylin (active) Leptin Adiponectin Leptin Adiponectin Leptin Proinsulin (total) Proinsulin (total) SAA-3 Molecular Weight) Adiponectin (High N-Propeptide (PIIANP) Procollagen Type IIA serum free) Insulin (Animal Resistin Insulin Adiponectin PYY (total) Pancreatic Polypeptide Insulin Growth Hormone(GH) Growth Description Chemiluminescent assay Preferred assay formeasuringGLP-1 (active) Bulk packaging available —more onselectkits environmentally friendlyandsaves space(10kitequivalent) WHO WHO WHO WHO Multi-Species Multi-Species Multi-Species Multi-Species Mouse, Rat Human Mouse, Rat Human Mouse, Rat Human Human Mouse, Rat Human Rat Human Mouse, Rat Canine Human Human Rat Rat Mouse Mouse Canine Mouse Human Human Human Human Human Human Human Mouse, Rat Mouse, Rat Species Human Human Human Mouse, Rat Human, 49.4–12,000 pg/mL 31.25–2000 pg/mL 3.125–200 ng/mL 12.6–3000 pg/mL 100–5000 pg/mL 8.2–2000 pg/mL 8.2–2000 pg/mL 9.9–2400 pg/mL 1.56–200 ng/mL 25–2000 pg/mL 25–2000 pg/mL 10–2000 pg/mL 0.78–50 ng/mL 1.5–200 ng/mL 0.5–100 ng/mL 0.078-5 μg/mL 0.12-10 ng/mL See data sheet See data 0.1–10 ng/mL 0.2–10 ng/mL 0.2–20 ng/mL 0.2–30 ng/mL 0.2–30 ng/mL 0.16–5 ng/mL 0.2–10 ng/mL 0.7–50 ng/mL 0.02–2 ng/mL 4.9-10 ng/mL lot dependent 2–200 µU/mL 2–200 µU/mL Curve Range Curve 4.1–1000 pM 25–1600 pM 1–64 ng/mL 1–50 ng/mL 2–100 pM 1–100 pM 2–200 pM Standard Standard

0.078 μg/mL 0.003 ng/mL 0.85 μU/mL 0.04 ng/mL 0.24 ng/mL 50.0 pg/mL 0.05 ng/mL 0.08 ng/mL 15.0 pg/mL 0.05 ng/mL 0.21 ng/mL 10.0 pg/mL 30.0 ng/mL 0.02 ng/mL 10.0 pg/mL 0.07 ng/mL 12.3 pg/mL Sensitivity 1.0 μU/mL 0.3 ng/mL 2.5 ng/mL 4.2 pg/mL 0.2 ng/mL 4.2 pg/mL 8.0 pg/mL 0.4 ng/mL 0.2 ng/mL 3.5 pg/mL 0.5 ng/mL 0.2 ng/mL 0.1 ng/mL 0.2 ng/mL 6.5 pg/mL 0.14 pM 15.0 pM 1.0 pM 1.0 pM 0.7 pM 0.5 pM 150-300 μL 150-300 μL 20-50 μL Volume Sample 100 µL 50 µL 50 μL 10 µL 20 µL 20 µL 20 µL 20 µL 20 µL 50 µL 10 µL 50 µL 20 µL 25 µL 10 µL 50 µL 10 µL 10 µL 10 µL 10 µL 20 µL 10 µL 20 µL 50 µL 10 μL 10 µL 10 μL 20 μL 20 µL 20 μL 25 µL 10 μL 20 μL 2 μL 2 μL 5 µL

EZRMFGF21-26K EZHMWAN-65K EZHFGF23-32K EZHFGF21-19K EZPIIANP-53K EZMSAA3-12K EZRMCP2-21K EZRMGIP-55K EZGLPHS-35K EZGLP1T-36K EZHIASF-14K EZRMGH-45K EZMADP-60K EZHPYYT66K EZRGRA-90K EZHADP-61K EZRADP-62K EZRGRT-91K EZHGIP-54K EZGLP2-37K EZGRA-88K EZHCP-20K EZGLU-30K EZRMI-13K EZGRT-89K EZHPP-40K EZCCP-47K EZHL-80SK EZHPI-15K EZHA-52K EZML-82K EZHR-95K EGLP-35K EZRL-83K EZCL-31K EZHI-14K Cat. No.Cat. CYT294 CYT298 EZHFGF23-32BK EZRMGIP-55BK EZGLPHS-35BK Bulk Packaging EZGLP1T-36BK EZRMGH-45BK EZMADP-60BK EZHADP-61BK EZHGIP-54BK EZGRA-88BK EZHCP-20BK EZGLU-30BK EZRMI-13BK EZGRT-89BK EZHPP-40BK EZCCP-47BK EZHPI-15BK EZHA-52BK EZML-82BK EZHR-95BK EGLP-35BK EZHL-80BK EZRL-83BK EZHI-14BK Cat. No.*Cat. ELISAs AND RIAs FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.

ELISA / RIA NS400 NS830 NS170 CYT420 CYT306 CYT304 PI-12K EZHS40 EZHS42 RI-13K HI-14K XL-85K HL-81K GL-32K LPI-16K Cat. No. SRI-13K Cat. No. HPI-15K SHL-81K HCP-20K EZHS-SET PYY-67HK CCP-24HK EZBRAIN40 EZBRAIN42 PYYT-66HK GHRT-89HK HADP-61HK EZHNPY-25K GHRA-88HK GLP1T-36HK GLP1A-35HK EZBRAIN-SET RMPYY-68HK EZRMNPY-27K EZHS100B-33K 50 µL 50 µL 50 µL 50 µL 20 µL 50 µL 50 µL 50 µL 50 µL 50 µL 100 μL 100 µL 1-10 µL Sample Volume 5 µL 50 μL 50 µL 50 µL 50 µL 50 µL 50 µL 50 µL 50 µL 50 µL 50 µL 50 µL 300 µL 300 µL 100 µL Sample Volume <100 µL <100 µL 50–100 µL 100–200 µL MerckMillipore.com/elisa_ria | 37 MerckMillipore.com/elisa_ria 100 µL (20 µL)

4 pg/mL 5 pg/mL 4 pg/mL 5 pg/mL 2 pg/mL 3 ng/mL 3 pM 3 pM 2 pM 7.8 pg/mL 1.3 pg/mL 0.9 ng/mL 1.5 ng/mL Sensitivity 1 ng/mL 1 ng/mL 2 μU/mL 2 µU/mL 0.004 ng/mL 10-15 pg/mL 20 pg/mL 93 pg/mL 20 pg/mL 10 pg/mL 0.0585 ng/mL 7.8 pg/mL 0.1 ng/mL 0.1 ng/mL 0.5 ng/mL 2.5 µU/mL Sensitivity 15.6 pg/mL 0.15 ng/mL 0.02 ng/mL 0.05 ng/mL (78.1 pg/mL)

Standard Contains of EZHS40 and EZHS42 1 each 3–60 ng/mL Curve Range Contains of EZBRAIN40 and EZBRAIN42 1 each 0.01-2 ng/mL 5-1000 pg/mL 16–500 pg/mL 16–500 pg/mL 16–500 pg/mL 16–500 pg/mL 1.5-100 ng/mL 7.8–500 pg/mL 10–1000 pg/mL 0.9–62.5 ng/mL 2.7-2000 pg/mL 0.0293–15 ng/mL Standard 2–100 pM 10–500 pM 1–50 ng/mL 10–1000 pM 0.1–5 ng/mL Curve Range 2–200 μU/mL 2–200 µU/mL lot dependent lot dependent lot dependent lot dependent lot dependent lot dependent 0.1–10 ng/mL 0.02–1 ng/mL 20–400 pg/mL 0.5–100 ng/mL 0.05–10 ng/mL 2.5–250 µU/mL 0.156–20 ng/mL Human, Mouse, Rat Human, Mouse, Human Human Human Human Human Human Species Human Human, Rat Rat Mouse, Multi-Species Human, Mouse, Rat Human, Mouse, Rat Mouse, Human Human Human Canine Human Human Multi-Species Multi-Species Multi-Species Species Human Porcine Rat Multi-Species Rat Human Human Multi-Species Human Human Rat Mouse, Human Human Adiponectin C-Peptide C-Peptide (active) Ghrelin (active) GLP-1 (total) GLP-1 Glucagon α-Synuclein beta 1-40 Amyloid beta 1-42 Amyloid beta, Set Amyloid beta (Brain) 1-42 Amyloid beta (Brain), Set Amyloid PEDF (Pigment Epithelium- Factor) Derived Description (total) Ghrelin Insulin Insulin Insulin LisPro Insulin (sensitive) Insulin Specific Leptin Leptin Leptin (sensitive) Proinsulin PYY (3-36) PYY (total)PYY Amyloid beta (Brain) 1-40 Amyloid BDNF (Brain-Derived Neurotrophic Factor) (Neuropeptide Y) NPY Phosphorylated Neurofilament, Sandwich (pNF-H) Description GFAP (Glial Fibrillary GFAP Acidic Protein) GrowthNGF (Nerve Factor) (Neuropeptide Y) NPY S100B RIAs have long been considered a valuable, economical and accurate way to measure protein to measure accurate way economical and a valuable, long been considered RIAs have please consider material, from using radioactive away move researchers concentration. As many needs. research for your using our other high quality immunoassays Radioimmunoassays (RIAs) Radioimmunoassays Neuroscience ELISAsNeuroscience and disease states with normal complexities associated of the of the nervous picture Get a complete ELISAs. neurodegenerative neuropeptide and using our of biomarkers quantification with reliable system Millipore Brand ELISAs Brand Millipore & RIAs FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES. SEARCH OUR ELISAs ONLINE

ELISAs 38 |SigmaAldrich.com • • ELISA Designations parameters, ofAnalysis box andopen the PDF. “sample”intotheCertificate type all onasinglepage, sosearchyour atSigmaAldrich.com target . To viewprotocolsandperformance andveterinary research.metabolic WithsuchalargenumberofELISAs, itisimpossibletolistthem types. Avariety ofresearch areas are addressed, frominflammationandcardiovascular diseaseto We of>1,800ELISAsandEIAs, offeraportfolio covering adiverse rangeofspeciesandsample Sigma-Aldrich Brand ELISAs – Custom SandwichELISA – – Sandwich ELISA – – – Requires custommanufacture andvalidation plasma orsupernatant accommodate thechangefromserum, Some may bedesignated“lysate” to off-the-shelfassayStandard Cytokines ✔ ✔ ✔ ✔ ✔ ✔ ✔ ✔ ✔ ✔ Metabolic ✔ ✔ ✔ ✔ ✔ • • Cardiovascular – ELISA Cell-Based Phosphorylation – EIA – – – – phosphorylation eventsphosphorylation For useoncelllysates todetect Competitive ELISA Upcharge may apply ✔ ✔ Angiogenesis ✔ ✔ ✔ Signaling ✔ ✔ ✔ ELISA READER / WASHER FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.

BIOTEK® 40-301 Cat. No. 40-006 40-007 40-300 Cat. No. MerckMillipore.com/elisa_ria | 39 MerckMillipore.com/elisa_ria NEW NEW NEW ELx50 Magnetic 96-well Strip Washer ® 800 TS Absorbance Reader, 800 TS Absorbance Reader, 800 TS Absorbance Reader, 800 TS Absorbance Reader, NEW ® ® ® Precise, accurate and versatile, used for many used for many accurate and versatile, Precise, and including ELISA, protein applications, plus kinetics and other end-point assays, cell-based assays Basic, Gen 5 and Gen 5 options: Software Secure 630nmFlexible filter options; 450nm, 590nm, and 750nm as standard plate Optional absorbance test NIST traceable quick programming and for Color touchscreen simple operation data export for convenient USB flash drive Durable and high quality design ® Description BioTek Gen 5 Software Gen 5 Software Gen 5 Secure Basic BioTek BioTek 50 TS Plate Washer • • • • • • • ® 50 TS Magnetic 96-well automated strip washer ® 50 TS Plate Washer 50 TS Plate Washer ® Color touchscreen makes programming quick and easy makes Color touchscreen touch operation for washing full or partial plates Easy sensing liquid level and safe: Reliable greater even Automated switching of up to 3 buffers for automation maintenance routines for continued Automated, built-in operation reliable BioTek Description BioTek BioTek • • • • • the BioTek Replaces 800 TS Absorbance Reader 800 TS Absorbance ® For Merck Millipore and Sigma-Aldrich ELISAs and Sigma-Aldrich Merck Millipore For BioTek Partnershipwith BioTek 800 TS to introduce the pleased we are ELISA products, 1800 Supporting of over our collection your to addition a cost effective provide which together Washer and 50 TS Plate Reader Absorbance flow. ELISA work ELISA Reading and Washing Equipment in Equipment Washing and Reading ELISA Merck KGaA Frankfurter Strasse 250 64293 Darmstadt, Germany

MerckMillipore.com

To place an order or receive technical assistance in Europe, please call Customer Service: France: 0825 045 645 Spain: 901 516 645 Option 1 Germany: 069 86798021 Switzerland: 0848 645 645 Italy: 848 845 645 United Kingdom: 0870 900 4645

For other countries across Europe, please call: +44 (0) 115 943 0840 Or visit: MerckMillipore.com/offices For Technical Service visit: MerckMillipore.com/techservice

© 2018 Merck KGaA, Darmstadt, Germany and/or its affiliates. All Rights Reserved. Merck, the Vibrant M, MILLIPLEX, MAPmate, SMC and SMCxPRO are trademarks of Merck KGaA, Darmstadt, Germany or its affiliates. All other trademarks are the property of their respective owners. Detailed information on trademarks is available via publicly accessible resources.

Lit. No. PF1089EN00MK 10/2018