AICDA (Activation-Induced Cytidine Deaminase, AID, Cytidine Aminohydrolase) (AP)

AICDA (Activation-induced Cytidine Deaminase, AID, Cytidine Aminohydrolase) (AP)

Catalog number 123101-AP Supplier United States Biological

Activation-induced cytidine deaminase (AICDA) was initially discovered as a homolog of the apolipoprotein B RNA-editing cytidine deaminase 1 (APOBEC1) that showed cytidine deaminase properties in stimulated B cell lines. It is necessary for somatic hypermutation and class switch recombination (CSR) in B cells, but inappropriate or dysregulated expression AICDA is often found in tumors and B cell neoplasms. Although it is structurally and functionally similar to the APOBEC proteins, it appears unlikely that AICDA deaminates dC to dU residues in HIV cDNA as does APOBEC3G. In bony fish such as zebrafish, the AICDA homologue also showed a similar function with mammalians, in which AICDA protein can mediate CSR. Applications Suitable for use in Western Blot. Other applications not tested. Recommended Dilution Optimal dilutions to be determined by the researcher. AA Sequence MDSLLMNRRKFLYQFKNVRWAKGRRETYLCYVVKRRDSATSFSLDFGYLRNKNGCHVELLFLRYISDWDLDPGRC YRVTWFTSWSPCYDCARHVADFLRGNPNLSLRIFTARLYFCEDRKAEPEGLRRLHRAGVQIAIMTFKDYFYCWNTFV ENHERTFKAWEGLHENSVRLSRQLRRILLPLYEVDDLRDAFRTLGL Storage and Stability Store product at 4°C. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Note Applications are based on unconjugated antibody. Immunogen Full length human AICDA, aa1-198 (AAH06296.1). Formulation Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phophatase (AP). Purity Purified by Protein A affinity chromatography. Specificity Recognizes human AICDA. Product Type Pab Source human Isotype IgG Grade Affinity Purified Applications WB Crossreactivity Hu Storage 4°C Do Not Freeze Detection Method AP Conjugate x