bioRxiv preprint doi: https://doi.org/10.1101/2020.11.04.367797; this version posted November 7, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. Glypicans specifically regulate Hedgehog signaling through their interaction with Ihog in cytonemes Eléanor Simon1, Carlos Jiménez-Jiménez1, Irene Seijo-Barandiarán1, Gustavo Aguilar1,2, David Sánchez-Hernández1, Adrián Aguirre-Tamaral1, Laura González-Méndez1, Pedro Ripoll1 and Isabel Guerrero1* 1) Tissue and Organ Homeostasis, Centro de Biología Molecular "Severo Ochoa" (CSIC- UAM), Nicolás Cabrera 1, Universidad Autónoma de Madrid, Cantoblanco, E-28049 Madrid, Spain. 2) Growth and Development, Biozentrum, University of Basel, Switzerland. * Corresponding author:
[email protected] Key words: Hedgehog Signaling, Cytonemes, Ihog, Glypicans, Dally, Dally-like protein 1 bioRxiv preprint doi: https://doi.org/10.1101/2020.11.04.367797; this version posted November 7, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. Abstract The conserved family of Hedgehog (Hh) signaling proteins plays a key role in cell-cell communication in development, tissue repair and cancer progression. These proteins can act as morphogens, inducing responses dependent on the ligand concentration in target cells located at a distance. Hh proteins are lipid modified and thereby have high affinity for membranes, which hinders the understanding of their spreading across tissues.