Polyclonal Antibody to BANP / SMAR1 (Center) - Aff - Purified

OriGene Technologies, Inc. OriGene Technologies GmbH

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AP26271PU-N Polyclonal Antibody to BANP / SMAR1 (Center) - Aff - Purified

Alternate names: BEN domain-containing protein 1, BEND1, Btg3-associated nuclear protein, Scaffold/matrix-associated region-1-binding protein Quantity: 0.1 mg Background: BANP was initially identified as a binding protein to BTG3 in a yeast two-hybrid screen. BANP acts as a tumor suppressor by stabilizing p53 expression and leading to cell cycle arrest. p53 in turn binds to upstream elements of the BANP promoter, thereby forming a feedback loop. BANP is down-regulated in advanced stages of human breast cancer, and its overexpression in breast cancer cell lines inhibits their ability to metastasize by modulating TGF-b signaling. Furthermore, BANP can modulate NF-kB transactivation and can inhibit tumorigenesis by regulating NF-kB target genes. Recent experiments have shown that BANP can also repress HIV-1 LTR mediated transcription by tethering the LTR matrix attachment region to nuclear matrix. Uniprot ID: Q8N9N5 NCBI: NP_001167010 GeneID: 54971 Host: Rabbit Immunogen: 14 amino acid peptide near the center of human BANP Format: State: Liquid Ig fraction Purification: Affinity chromatography purified via peptide column Buffer System: Antibody is supplied in PBS containing 0.02% sodium azide Applications: ELISA. Western blot: 1-2 µg/ml. Positive control: Mouse Kidney Tissue Lysate. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. Specificity: This antibody detects BANP / SMAR1 at Center. Species Reactivity: Tested: Human, mouse, rat Storage: Store at 2 - 8 °C for up to three months or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. General Readings: 1. Birot A, Duret L, Bartholin L, Santalucia B, Tigaud I, Magaud J, et al. Identification and molecular analysis of BANP. Gene. 2000 Aug 8;253(2):189-96. PubMed PMID: 10940556. 2. Kaul R, Mukherjee S, Ahmed F, Bhat MK, Chhipa R, Galande S, et al. Direct interaction with and activation of p53 by SMAR1 retards cell-cycle progression at G2/M phase and delays tumor growth in mice. Int J Cancer. 2003 Feb 20;103(5):606-15. PubMed PMID: 12494467. 3. Singh K, Mogare D, Giridharagopalan RO, Gogiraju R, Pande G, Chattopadhyay S.

For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. OG/20160219 1 / 2 AP26271PU-N: Polyclonal Antibody to BANP / SMAR1 (Center) - Aff - Purified

p53 target gene SMAR1 is dysregulated in breast cancer: its role in cancer cell migration and invasion. PLoS One. 2007 Aug 1;2(7):e660. PubMed PMID: 17668048. 4. Singh K, Sinha S, Malonia SK, Bist P, Tergaonkar V, Chattopadhyay S. Tumor suppressor SMAR1 represses IkappaBalpha expression and inhibits p65 transactivation through matrix attachment regions. J Biol Chem. 2009 Jan 9;284(2):1267-78. doi: 10.1074/jbc.M801088200. Epub 2008 Nov 3. PubMed PMID: 18981184. Pictures: Western blot analysis of BANP in mouse kidney tissue lysate with BANP antibody at (A) 1 and (B) 2 ug/mL.

Immunohistochemistry of BANP in human kidney tissue with BANP antibody at 10 ug/mL.

Immunofluorescence of BANP in human kidney tissue with BANP antibody at 20 ug/mL.

For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. OG/20160219 2 / 2