SANTA CRUZ BIOTECHNOLOGY, INC. A (JC159): sc-53295

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BACKGROUND SOURCE A, B and C are sialoglycoproteins of the human erythrocyte (JC159) is a mouse monoclonal antibody raised against membrane, which bear the antigenic determinants for the MN, Ss and Gerbich membrane preparation from splenic hairy cell leukaemia cells. blood groups, respectively. Glycophorins span the membrane once and present their amino-terminal end to the extracellular surface of the human erythrocyte. PRODUCT The genetic array of expressed glycophorin surface antigens on erythrocytes Each vial contains 200 µg IgG1 in 1.0 ml of PBS with < 0.1% sodium azide defines the blood group phenotype of the individual. The human glycophorin and 0.1% gelatin. A maps to 4q28.2-q31.1, contains seven exons which are 97% homologous to glycophorin B, and encodes a 150 amino acid . APPLICATIONS The human glycophorin B gene maps to chromosome 4q28-q31 and encodes a 91 amino acid protein. The human gene maps to chromosome Glycophorin A (JC159) is recommended for detection of Glycophorin A of 2q14-q21 and contains four exons. Glycophorin C transcript can generate two human and rat origin by immunofluorescence and immunohistochemistry protein isoforms. Isoform 1 includes all four exons and encodes the full length (including paraffin-embedded sections) (starting dilution 1:50, dilution range 128 amino acid protein. Isoform 2 is missing exon 2 and encodes a 109 amino 1:50-1:500). acid protein, which specifies the Yus subtype of the Gerbich phenotype. Suitable for use as control antibody for Glycophorin A siRNA (h): sc-42882. Molecular Weight of Glycophorin A: 16/38 dimers kDa. REFERENCES 1. Andersson, L.C., Gahmberg, C.G., Teerenhovi, L. and Vuopio, P. 1979. RECOMMENDED SECONDARY REAGENTS Glycophorin A as a cell surface marker of early erythroid differentiation in To ensure optimal results, the following support (secondary) reagents are acute leukemia. Int. J. Cancer 23: 717-720. recommended: 1) Immunofluorescence: use goat anti-mouse IgG-FITC: 2. Liszka, K., Majdic, O., Bettelheim, P. and Knapp, W. 1983. Glycophorin A sc-2010 (dilution range: 1:100-1:400) or goat anti-mouse IgG-TR: sc-2781 expression in malignant hematopoiesis. Am. J. Hematol. 15: 219-226. (dilution range: 1:100-1:400) with UltraCruz™ Mounting Medium: sc-24941. 3. Greaves, M.F., Sieff, C. and Edwards, P.A. 1983. Monoclonal antigly- 2) Immunohistochemistry: use ImmunoCruz™: sc-2050 or ABC: sc-2017 cophorin as a probe for erythroleukemias. Blood 61: 645-651. mouse IgG Staining Systems. 4. Villeval, J.L., Cramer, P., Lemoine, F., Henri, A., Bettaieb, A., Bernaudin, F., STORAGE Beuzard, Y., Berger, R., Flandrin, G. and Breton-Gorius, J. 1986, Phenotype Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of of early erythroblastic leukemias. Blood 68: 1167-1174. shipment. Non-hazardous. No MSDS required. 5. Loken, M.R. Shah, V.O., Dattilio, K.L. and Civin, C.I. 1987. Flow cytometric analysis of human bone marrow: I. Normal erythroid development. Blood RESEARCH USE 69: 255-263. For research use only, not for use in diagnostic procedures. 6. Loken, M.R., Shah, V.O., Dattilio, K.L. and Civin, C.I. 1987. Flow cytometric analysis of human bone marrow. II. Normal B lymphocyte development. PROTOCOLS Blood 70: 1316-1324 See our web site at www.scbt.com or our catalog for detailed protocols 7. Cuneo, A., Van Orshoven, A., Michaux, J.L., Boogaerts, M., Louwagie, A., and support products. Doyen, C., Dal Cin, P., Fagioli, F., Castoldi, G. and Van den Berghe, H. 1990. Morphologic, immunologic and cytogentic studies in erythroleukemia: evi- dence for multilineage involvement and identification of two distinct cyto- genetic-clinicopathological types. Br. J. Haematol. 75: 346-354. 8. Erber, W.N., McLachlan, J., Cordell, J.L., Turley, H., Reid, M. and Mason, D.Y. 1991. A new monoclonal antibody (JC159) that detects glycophorin A for the diagnosis of erythroleukaemia. Hematol. Rev. 5: 113-120 9. Sadahira, Y., Kanzaki, A., Wada, H. and Yawata, Y. 1999. Immunohisto- chemical identification of erythroid precursors in paraffin embedded bone marrow sections: is a superior marker to glycophorin. J. Clin. Pathol. 52: 919-921.

CHROMOSOMAL LOCATION Genetic locus: GYPA (human) mapping to 4q28.2-q31.1; Gypa (mouse) mapping to 8 C2.

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