20Th Coordinators Meeting
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Immunology, Immunity, Inflammation & Immunotherapies
2231st Conference Immunology Summit & Immunity and Immunotherapies 2018 10th World Congress and Expo on Immunology, Immunity, Inflammation & Immunotherapies October 19-20, 2018 | New York, USA Poster Presentations Page 45 Daniel Horowitz et al., J Clin Cell Immunol 2018, Volume: 9 DOI: 10.4172/2155-9899-C4-058 10th World Congress and Expo on Immunology, Immunity, Inflammation & Immunotherapies October 19-20, 2018 | New York, USA The development of a novel 3D printed prototype device to simplify blood cell subset enrichment at clinical sites Daniel Horowitz, George Szabo, Rudolf Cedro Jr. and Ling-Yang Hao Janssen Pharmaceutical Companies of Johnson & Johnson, USA eripheral blood collection is a relatively non-invasive way to obtain biomarkers during clinical trials. Protein and gene Pexpression changes can be used to assess drug/target engagement and disease state changes. To avoid functional changes in blood cells during storage, cells need to be stored in appropriate buffers. For gene expression analysis, whole blood can be collected in buffers that lyse the cells and protect RNA from degradation, but this obscures changes that may be found only in specific cell types. The separation of specific subsets requires instrumentation and trained personnel not readily available at many clinical sites, and shipping to a processing lab may affect the results. Therefore, a way to enrich for and stabilize blood cell subsets at the site of collection is needed. We have developed a portable 3D printed prototype device that can be operated with minimal training. Blood is collected into a tube containing commercially available polystyrene spheres (PluriSelect) coupled to an antibody specific for a blood cell surface antigen. -
Arun Chauhan, Ph.D. Assistant Research Professor University Of
Arun Chauhan, Ph.D. Assistant Research Professor University of North Dakota, Grand Forks, ND, 58202 Phone: (701) 215-2044 Email: [email protected] (US Permanent Resident) SUMMARY OF QUALIFICATIONS Wide-ranging experience in Immunology, Oncology, Molecular Biology and infectious disease with strong expertise in disease Model. Experience in immune cell isolation, innate cell purification, co-culture assays, cytokine (ELISA, CBA) and cell proliferation, Cytotoxic assay. Extensive experience in molecular biology techniques such as, RT-PCR, western blot, Nucleic acid Extraction, Quantitative PCR, DNA footprinting assay, and recombinant DNA technology). Strong expertise in cell based immunological assays (ELISPOT), Reporter assay, transplantation, multiparametric flow cytometry, FACS, Intracellular Cytokine staining. Experience in mammalian primary cell Culture, & primary human lymphocyte (T cells, B cells, DCs and macrophages) from blood, si/shRNA transfection, CRISPR/Cas9. Successfully initiated, managed and completed multiple projects simultaneously and published research in peer-reviewed journals. Competent to work independently and in team-based projects as evident from more than 20 publications. Ability to critically analyze and troubleshoot data, interpret results and effectively present findings in meetings. Excellent organizational, and written, oral and interpersonal communication skills. PROFILE Interdisciplinary immunology, Molecular, Epigenetics-scientist with an in-depth expertise in in vitro and in vivo cell-based assays (murine and human), involving macrophages, Neutrophils and T- cells. Development of murine models to investigate the role of macrophage plasticity and T-cell functionality in Sjogren’s Syndrome and Pneumonic Sepsis (2° Infection) in fate mapping mice. Proven expertise in genome molecular biology (RNA-Seq, ChIP Seq, ATAC seq & short capped RNA-seq). Proven ability to complete projects in time-bound manner. -
Cancer Immunology and Immunotherapy
UC Irvine UC Irvine Previously Published Works Title Cancer Immunology and Immunotherapy. Permalink https://escholarship.org/uc/item/67q1k6k3 Authors Owais, Mohammad Zubair, Swaleha Agrawal, Anshu et al. Publication Date 2015 DOI 10.1155/2015/393454 Peer reviewed eScholarship.org Powered by the California Digital Library University of California Hindawi Publishing Corporation BioMed Research International Volume 2015, Article ID 393454, 2 pages http://dx.doi.org/10.1155/2015/393454 Editorial Cancer Immunology and Immunotherapy Mohammad Owais,1 Swaleha Zubair,1 Anshu Agrawal,2 and Yung-Fu Chang3 1 AligarhMuslimUniversity,Aligarh,India 2University of California, Irvine, USA 3Cornell University, Ithaca, USA Correspondence should be addressed to Mohammad Owais; owais [email protected] Received 10 May 2015; Accepted 10 May 2015 Copyright © 2015 Mohammad Owais et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The study of molecular and cellular interplays between of the newly introduced method with various published immune system and cancerous cells is gaining tremen- protocols to underline its effectiveness thereof. In the arena dous momentum across the globe. Concomitantly, with the of NK cell based immunotherapies, the article “NKG2D and better insight into the intricacies of cancer immunology, DNAM-1 Ligands: Molecular Targets for NK Cell-Mediated immunotherapeutic approaches to deal with cancer have Immunotherapeutic Intervention in Multiple Myeloma” by garnered tremendous boost in the recent past; reckoning C. Fionda et al. has come up with satisfactory results to with these, it is timely to analyse their potentialities either further potentiate NK cell based immunotherapies. -
Role of IL-18 and Its Signaling in Atherosclerosis
Inflammation & Cell Signaling 2015; 2: e707.doi: 10.14800/ics.707; © 2015 by Owais Mohammad Bhat, et al. http://www.smartscitech.com/index.php/ics REVIEW Role of IL-18 and its signaling in atherosclerosis Owais Mohammad Bhat, Veena Dhawan Department of Experimental Medicine and Biotechnology, Postgraduate Institute of Medical Education and Research (PGIMER), Chandigarh Correspondence: Veena Dhawan E-mail: [email protected] Received: March 11, 2015 Published online: April 22, 2015 Cardiovascular diseases (CVD) including coronary artery disease (CAD) and stroke are the largest cause of worldwide morbidity and mortality, where atherosclerosis is the underlying pathology. Recent investigations of atherosclerosis have focused on the role of inflammation, providing new insights into the mechanism of the disease. Macrophages and T-lymphocytes present in the atherosclerotic lesions produce a wide array of cytokines that can exert both pro- and anti-inflammatory effects. Pro-inflammatory cytokines of the interleukin category are considered to be key players in the chronic vascular inflammation that is typical for atherosclerosis. Various studies support the concept that interleukin-18 (IL-18) is a pro-inflammatory cytokine with pro-atherogenic properties. Previous data in Apo E-/- mice demonstrated that IL-18 accelerates atherosclerosis via interferon gamma (IFN- γ) and CXCL16 expression. IL-18 binds to its receptor IL-18R complex which is a heterodimer with α (IL-1Rrp) chain responsible for extracellular binding of IL-18 and a nonbinding, signal-transducing β (AcPL) chain. By binding to IL-18Rα, IL-18 upregulates IL-1R-associated kinase (IRAK) and TRAF-6 thus, results in nuclear translocation of nuclear factor kappa-B (NF-κB). -
Awardees of National Bioscience Award for Career Development
AWARDEES OF NATIONAL BIOSCIENCE AWARD FOR CAREER DEVELOPMENT Awardees for the year 2016 1. Dr. Mukesh Jain, Associate Professor, School of Computational and Integrative Sciences, Jawaharlal Nehru University, New Delhi-110067 2. Dr. Samir K. Maji, Associate Professor, Indian Institute of Technology, Powai, Mumbai- 400076 3. Dr. Anindita Ukil, Assistant Professor, Calcutta University, Kolkata 4. Dr. Arnab Mukhopadhyay, Staff Scientist V, National Institute of Immunology, Aruna Asaf Ali Marg, New Delhi- 110067 5. Dr. Rohit Srivastava, Professor, Indian Institute of Technology, Bombay, Mumbai- 400076 6. Dr. Pinaki Talukdar, Associate Professor, Indian Institute of Science Education and Research, Dr. Homi Bhabha Road, Pashan, Pune- 7. Dr. Rajnish Kumar Chaturvedi, Senior Scientist, CSIR- Indian Institute of Toxicology Research, Lucknow-226001 8. Dr. Jackson James, Scientist E-II, Neuro Stem Cell Biology Lab, Neurobiology Division, Rajiv Gandhi Centre for Biotechnology, Thiruvananthapuram, Kerala- 695014 Awardees for the year 2015 1. Dr. Sanjeev Das, Staff Scientist-V, National Institute of Immunology, New Delhi 2. Dr. Ganesh Nagaraju, Assistant Professor, Department of Biotechnology, Indian Institute of Science, Bangalore- 5600012. 3. Dr. Suvendra Nath Bhattacharya, Principal Scientist, CSIR- Indian Institute of Chemical Biology, Kolkata- 700032 4. Dr. Thulasiram H V, Principal Scientist, CSIR-National Chemical Laboratory, Pune- 411008. 5. Dr. Pawan Gupta, Principal Scientist, Institute of microbial Technology, Chandigarh- 160036. 6. Dr. Souvik Maiti, Principal Scientist, CSIR-Institute of Genomics and Integrative Biology, Delhi- 110025. 7. Dr. Pravindra Kumar, Associate Professor, Department of Biotechnology, IIT, Roorkee- 247667. 8. Dr. Anurag Agrawal, Principal Scientist, CSIR-Institute of Genomics and Integrative Biology, Delhi- 110025 9. Dr. Gridhar Kumar Pandey, Professor, Department of Plant Molecular Biology, University of Delhi South Campus, New Delhi- 110067 10. -
National Bioscience Awards for Career Development
AWARDEES OF NATIONAL BIOSCIENCE AWARDS FOR CAREER DEVELOPMENT Awardees for the year 2012 1. Dr. Kaustuv Sanyal, Associate Professor, Molecular Mycology Laboratory, Molecular Biology & Genetics Unit, Jawaharlal Nehru Centre for Advance Scientific Research, Jakkur P.O. Bangalore 560064 2. Dr Naval Kishore Vikram, Associate Professor, Department of Medicine, All India Institute of Medical Sciences (AIIMS), Ansari Nagar, New Delhi- 110029 3. Dr. Aditya Bhushan Pant, Senior Scientist & In-charge, In Vitro Toxicology Laboratory, Indian Institute of Toxicology Research, PO Box: 80, MG Marg, Lucknow 226001 (UP) India 4. Dr. Subrata Adak, Senior Scientist, Indian Institute of Chemical Biology; 4, Raja S.C. Mullick Road, Kolkata-700032 5. Dr. Durai Sundar, Assistant Professor, Dept of Biochemical Engineering & Biotechnology, Indian Institute of Technology (IIT) Delhi, Hauz Khas, New Delhi – 110016 6. Dr S Venkata Mohan, Senior Scientist, Bioengineering and Environmental Centre (BEEC) CSIR-Indian Institute of Chemical Technology, Hyderabad-500 607 7. Dr. Munia Ganguli, Scientist E-I, CSIR-Institute of Genomics & Integrative Biology, Mall Road,New Delhi 110 007 8. Dr. Asad U Khan, Associate Professor & Coordinator/Head of Biotechnology Department, A.M.U, Interdisciplinary Biotechnology Unit, A.M.U., Aligarh 202002 9. Dr. Sathees C. Raghavan, Assistant Professor, Department of Biochemistry, Indian Institute of Science, Bangalore 560 012 10. Dr. Vidita A. Vaidya, Associate Professor, Department of Biological Sciences, Tata Institute of Fundamental Research, 1, Homi Bhabha Road, Colaba, Mumbai - 400005 Awardees for the year 2011 1. Dr. M. M. Parida, Scientist-F, Joint Director, Division of Virology Defence R & D Establishment, DRDE, DRDO, Ministry of Defence, Jhansi Road, Gwalior- 474002 2. -
In Vitro Anti-Inflammatory Activity of Ginger (Zingiber Officinale Rosc.) Rhizome, Callus and Callus Treated with Some Elicitors
Vol. 13(10), pp. 227-235, 25 May, 2019 DOI: 10.5897/JMPR2019.6758 Article Number: B19AD1C61046 ISSN 1996-0875 Copyright © 2019 Author(s) retain the copyright of this article Journal of Medicinal Plants Research http://www.academicjournals.org/JMPR Full Length Research Paper In vitro anti-inflammatory activity of ginger (Zingiber officinale Rosc.) rhizome, callus and callus treated with some elicitors Ammar Mohammed Ahmed Ali1,2*, Mawahib ElAmin Mohamed El-Nour2, Owais Mohammad3 and Sakina Mohamed Yagi4 1Department of Biology, Faculty of Education, Hajjah University, Yemen. 2Department of Biology and Biotechnology, Faculty of Science and Technology, AL Neelain University, Khartoum, Sudan. 3Molecular Immunology Lab1, Biotechnology Unit, Aligarh Muslim University, Aligarh, India. 4Department of Botany, Faculty of Science, University of Khartoum, Sudan. Received 12 March, 2019; Accepted 30 April, 2019 This study evaluated the effect of ginger rhizome and its callus as well as callus elicited by yeast extract, glycine and salicylic acid on the production of pro-inflammatory (TNF-α, IL-1 and IL-6) and anti- inflammatory (IL-10 and TGF-β) cytokines in vitro. Petroleum ether (PE) and chloroform; methanol (1:1) (CM) extracts of rhizome and callus were shown to significantly (P < 0.05) suppress in a dose- dependent manner the LPS-induced production of TNF-α, IL-1 and IL-6. Both callus extracts showed significantly (P < 0.05) higher ability than the rhizome extracts. CM extract of ginger callus treated with elicitors showed significant (p < 0.05) capacity to inhibit IL-1, IL-6 and TNF-α secretion at highest concentration used (100 μg/mL) when compared to control (untreated callus). -
Editorial Novel Vaccine Adjuvants
UC Irvine UC Irvine Previously Published Works Title Novel Vaccine Adjuvants Permalink https://escholarship.org/uc/item/80c7q63x Journal BioMed Research International, 2013 ISSN 2314-6133 2314-6141 Authors Agrawal, Anshu Owais, Mohammad Singh, Udai P Publication Date 2013 DOI 10.1155/2013/835105 License https://creativecommons.org/licenses/by/4.0/ 4.0 Peer reviewed eScholarship.org Powered by the California Digital Library University of California Hindawi Publishing Corporation BioMed Research International Volume 2013, Article ID 835105, 2 pages http://dx.doi.org/10.1155/2013/835105 Editorial Novel Vaccine Adjuvants Anshu Agrawal,1 Mohammad Owais,2 and Udai P. Singh3 1 Division of Basic and Clinical Immunology, Department of Medicine, University of California, Irvine, CA 92697, USA 2 Interdisciplinary Biotechnology Unit, Aligarh Muslim University, Aligarh, Uttar Pradesh 202002, India 3 Department of Pathology, Microbiology and Immunology, University of South Carolina, School of Medicine, Building 1, Room B 42, 6439 Garners Ferry Road, Columbia, SC 29208, USA Correspondence should be addressed to Anshu Agrawal; [email protected] Received 11 September 2013; Accepted 11 September 2013 Copyright © 2013 Anshu Agrawal et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Vaccines still remain the most successful method for pro- fibronectin (EDA) targets antigens to TLR4-expressing cells tection and eradication against diseases. However, to avoid such as DCs leading to their activation. The approach is harmful effects associated with whole organism vaccines, new also effective for cross-presentation as well as the induction vaccine candidates are composed of parts of an organism, of antiviral/tumor immunity. -
Maulana Azad Library, Aligarh Muslim University
Maulana Azad Library, Aligarh Muslim University Date…………… CERTIFICATE This is to certify that the thesis entitled “Immune potential of Mycobacterium tuberculosis hypoxic stress induced Acr-1 protein against intracellular Mycobacterium species infection” herewith submitted by Ms. Nida in fulfilment of the requirements for the degree of Doctor of Philosophy in Biotechnology of the Aligarh Muslim University, is an authentic record of the research work carried out by her under my supervision and guidance and that no part, thereof, has been presented before for any other degree. Prof. Mohammad Owais (Supervisor) Maulana Azad Library, Aligarh Muslim University INTERDISCIPLINARY BIOTECHNOLOGY UNIT ALIGARH MUSLIM UNIVERSITY ALIGARH- 202002 INDIA ANNEXURE – I SELF DECLARATION I, Ms. Nida, Interdisciplinary Biotechnology Unit, certify that the work embodied in this Ph.D. thesis is my own bonafide work carried out by me under the supervision of Prof. Mohammad Owais at Aligarh Muslim University, Aligarh. The matter embodied in this Ph.D. thesis has not been submitted for the award of any other degree. I declare that I have faithfully acknowledged, given credit to and referred to the research workers whenever their works have been cited in the text and in the body of the thesis. I further certify that I have not plagiarised some other work, paragraph, text, data, results, etc. reported in the journals, books, magazines, reports, dissertations, thesis, etc. or available on web-sites, and included them in this Ph.D. thesis or cited them as my own work. Date: …………… (Signature of the Candidate) NIDA …..……………………………………………………………………………………… Certificate from the Supervisor This is to certify that the above statement made by the candidate is correct to the best of my/our knowledge. -
Doctor of Philosophy in Biotechnology
Role of cytokines in eshtablisment and survival of intracellular pathogen in the host THESIS SUBMITTED FOR THE AWARD OF DEGREE OF Doctor of Philosophy In Biotechnology By Anzar Abdul Mujeeb Maulana Azad UnderLibrary, the Aligarh Supervision Muslim of University PROF. Owais Mohammad INTERDISCIPLINARY BIOTECHNOLOGY UNIT ALIGARH MUSLIM UNIVERSITY ALIGARH-202002 (INDIA) 2019 Dedicated to My Beloved Family & Friends Maulana Azad Library, Aligarh Muslim University Certificate This is to certify that the thesis entitled “Role of cytokines in establishment and survival of intracellular pathogen in the host” herewith submitted by Mr. Anzar Abdul Mujeeb in fulfilment of the requirements for the degree of Doctor of philosophy in Biotechnology of the Aligarh Muslim University, is an authentic record of the research work carried out by him under my supervision and guidance and that no part, thereof, has been presented before for any other degree. Prof. Owais Mohammad (Supervisor) Maulana Azad Library, Aligarh Muslim University INTERDISCIPLINARY BIOTECHNOLOGY UNIT ALIGARH MUSLIM UNIVERSITY, ALIGARH-202002 (INDIA) CANDIDATE’S DECLARATION I, Anzar Abdul Mujeeb, from Interdisciplinary Biotechnology Unit, certify that the work embodied in this Ph.D. thesis is my own bonafide work carried out by me under supervision of Prof. Owais Mohammad at Aligarh Muslim University, Aligarh, India. The matter embodied in this Ph.D. thesis has not been submitted for the award of any other degree. I declare that I have faithfully acknowledged, given credit to and referred to the research workers wherever their works have been cited in the text and the body of the thesis. I further certify that I have not willfully lifted up some other’s work, para, text, data, result, etc., reported in the journals, books, magazines, reports, dissertations, theses, etc., or available at websites and included them in this Ph.D. -
Immunology, Immunity, Inflammation & Immunotherapies
J Clin Cell Immunol 2018, Volume: 9 DOI: 10.4172/2155-9899-C4-058 10th World Congress and Expo on Immunology, Immunity, Inflammation & Immunotherapies October 19-20, 2018 | New York, USA Mycobacterium tuberculosis host cell interaction: Role of latency associated protein Acr-1 in differential modulation of macrophages Nida Mubin and Owais Mohammad Aligarh Muslim University, India ycobacterium tuberculosis (M.tb) contrives intracellular abode as a strategy to combat antibody onslaught. Additionally, to Mthrive against hostile ambiance inside host macrophages, the pathogen inhibits phagolysosomal fusion. Finally, to further defy host cell offensives, M.tb opts for dormant phase, where it turns off or slows down most of its metabolic process as an added stratagem. While M.tb restrains most of its metabolic activities, surprisingly latency-associated alpha-crystallin protein (Acr-1) is expressed most prominently during dormancy. Interestingly, several previous studies described the potential of Acr-1 to induce a robust immuno-prophylactic response in the immunized host. It is intriguing to comprehend the apparent discrepancy that how the microbe M.tb overexpresses a protein that has the potential to prime host immune system against the pathogen itself. Keeping this apparent ambiguity into consideration, it is imperative to unravel the intricacies involved in the exploitation of Acr-1 by M.tb during its interaction with host immune cells. The present study suggests that Acr-1 exhibits a diverse role in the maturation of macrophages (MΦs) and related immunological responses. The early encounter (pre-exposure) of bone marrow-derived immune cells (during their differentiation to MΦs) with Acr-1 (AcrMΦpre), results in hampering of their function. -
A101.Full.Pdf
COMPLICATIONS—HYPOGLYCEMIA COMPLICATIONS—HYPOGLYCEMIA ± 10 pg/ml; P < 0.01), and endogenous glucose production improved from baseline to 6 and 18 months (0.42 ± 0.08 to 0.47 ± 0.04 and 0.87 ± 0.20 mg/ kg/min; P < 0.05), albeit remaining less than normal (1.39 ± 0.11 mg/kg/min; Moderated Poster Discussion: Risks for and Consequences of Hypogly- P < 0.05); there was no improvement in epinephrine or the autonomic symp- cemia (Posters: 382-P to 387-P), see page 21. tom score. Real-time CGM can reduce the burden of problematic hypogly- cemia in patients with long standing T1D complicated by hypoglycemia & 382-P unawareness, which may in part be related to improvement in the endog- enous glucose production response to insulin-induced hypoglycemia that is Critical Hypoglycemia in Hospitalized Patients with Diabetes: Early POSTERS required to prevent or correct low blood glucose. Complications Outcomes of Standardized Reporting and Management Supported By: National Institutes of Health (R01 DK091331, UL1 TR000003, P30 Acute and Chronic KATHERINE A. ARAQUE, DEEPAK KADAYAKKARA, NINO GIGAURI, KASIA J. LIP- DK19525, T32 DK007314) SKA, GREGORY BULLER, CLARE A. FLANNERY, Bridgeport, CT, New Haven, CT Critical hypoglycemia (glucose <50 mg/dl) is associated with increased morbidity and mortality in hospitalized patients. We performed a root cause & 384-P analysis of critical hypoglycemia events (HGE) on two inpatient medical Risk of Cardiac Arrhythmias during Spontaneous Hypoglycemia in wards with the highest incidence of HGE. Pre-intervention, management Young People with Type 1 Diabetes and reporting of HGE were inconsistent, and time from HGE to documented PETER NOVODVORSKY, ALAN BERNJAK, ELAINE CHOW, AHMED IQBAL, LIANNE euglycemia (>100 mg/dl) was over 3 hours.