PATHWAYS & KEY MOLECULAR TARGETS
TGF-β Signaling
CANONICAL NON-CANONICAL Differential / Context Dependent TGF-β - SMADs Signaling in Normal and Cancer Cells PATHWAY TGF-β TGF-β PATHWAY TGF-β R I/ NORMAL CELL CANCER CELL Latent TGF-β ALK-5 In normal cells, the activated SMADs translocate into the cell’s nucleus wherein they drive antitumor In cancer cells, p53, PTEN or SMAD4 loss (dotted shapes) favors different signaling mechanisms which effects by inhibiting cell proliferation and inducing apoptosis. drive cell proliferation, apoptotic resistance and EMT/metastasis. TGF-β TGF-β R I/ALK-5 Plasmin, TSP TGF-β Receptors TGF-β R II TGF-β Complex TGF-β Formation TGF-β R II Ligand
P P P P Tyrosine P TGF-β R I/ALK-5 TGF-β R II TGF-β R II Kinase LTBP P P TGF-β R I/ALK-5 TGF-β P Receptor TGF-β R II Plasma Membrane Plasma Membrane SEMA TGF-β P SMURF1/2 TAK1 P P P P SMAD-2/3 P PTEN P P IKK-α RAS P IKK- TGF-β R III β PI3K P IKK-γ RhoA P X SMAD6/7 P SEMA SEMA P I-SMADs BRAF TAK1 RAF P SMAD-3 IkB SMAD-3 PTEN AKT P ALK-1 RAS ROCK CDK PDK1 SMAD-3 SMAD-4 P SMAD-2/3 P P co-SMAD R-SMADs P MAPK SMURF1/2 RelA/p65 p50 SMAD-3 Endoglin SMAD-4 P IkB JNK p38 ERK P P LIMK X TGF-β R II P P SMAD-4 SMAD-3 P P PCB1 Wnt-β-catenin signaling SMAD-4 SMAD-1/5/8 AKT CFL1 P P P P SMAD-3 R-SMADs IkB Degradation SMAD-4 SMURF1/2 mTOR Cytoplasm Cytoplasm Nucleus P P Nucleus SMAD-3 DROSHA SMAD-1/5/8 P p21 SOX4 PTHrP P SMAD-2/3 P Inhibition RelA/p65 Cellular Growth, p50 GTPases of Cell Induction SOX4 Resistance to Signaling p15 Growth KLF5 of Apoptosis EMT & Cancer Metastasis SMAD-4 Apoptosis, S6K via pre-miRNA Processing P EMT/Metastatic P P P SMAD-3 P P Progression PCB1 CBP SMAD-3 SMAD-3 SMAD-4 AKT CBP EMT & Metastasis via p53 Signaling SMAD-4 TF* CBP Alternative Splicing SMAD-4 KLF5 SMAD-3 P p53 P SMAD-1/5/8 P TGF-β Target Genes Target Genes of TGF-β Signaling Small Molecule Inhibitors of TGF-β Signaling SMAD4 Degradation NFkB Processes Target Genes Small Molecule Description Signaling P P SMAD-2/3 RelA/p65 p50 CTGF, FN1, ID1, IL6, MMP2, MMP9, SERPINE1/PAI1, LY 364947 Selective inhibitor of TGF-βRI TF* Angiogenesis CBP SMAD-4 CBP TSP, VEGFA BCL2, BCL2L1/BCL-XL, BIK, BCL2L11/BIM, EIF2A, SB 525334 Selective inhibitor of TGF-βRI TGF-β JNK p38 ERK Apoptosis FAS, ERN1, EIF2AK3/PERK, MAPK8/JNK, SNAI1/Snail, Target SB 505124 Selective inhibitor of TGF-βRI, ALK4 and ALK7 Genes SOX4
SMAD-4 TF* AMPK, AKT, ATG5, ATG7, BECN1, DAPK1, mTOR, TAB1, GW 788388 Potent and selective inhibitor of TGF-βRI P Autophagy c-Jun c-Fos c-Myc TAK1 SMAD-1/5/8 CBP SD 208 Potent ATP-competitive TGF-βRI inhibitor CBP ATF2 4EBP1/EIF4EBP1, AKAP12, ID1, ID2, MYC, p15/ *TF: AP1, RUNX, FOX, bHLH, SP1, IRF7 Cell Proliferation INK4B/ CDKN2B, p21/CIP1/CDKN1A, p57/KIP2/ R 268712 Potent and selective inhibitor of TGF-βRI CDKN1C, PDGFB, PI3K Nucleus Epithelial to ACTN1, CFL1/CFL, COL4A1, HMGCA, MMP2, MMP9, IN 1130 Potent and selective inhibitor of TGF-βRI Mesenchymal NET1, SNAI1/Snail, SNAI2/Slug, TWIST, ZEB1, ZEB2 Cytoplasm Plasma Membrane Transition SB 431542 Potent, selective inhibitor of TGF-βRI, ALK4 and ALK7 Pathway Key: Shape Colors Pathway Key: Connectors Extra-Cellular Matrix ACTA2/α-SMA, COL1A1, COL4A1, CTGF, FN1, LAMC2, A 83-01 Selective inhibitor of TGF-βRI, ALK4 and ALK7 (ECM) Synthesis SPOK1 Direct Activation Ligands FASL, FOXP3, GZMA, GZMB, IFNG/IFG, IgA, IL11, IL17, RepSox Potent and selective inhibitor of TGF-βRI Immune Response Direct Inhibition Receptors IL2, IL6, KLRK1/NKG2D, PRF1, TNFA ITD 1 Selective inhibitor of TGF-β signaling Multi-step Regulation Kinases Self-Renewal/ CTNNB1, HGF, KLF4, LIF, MET, NANOG, SOX2, SOX4, Stemness WNT5A SIS3 Selective Smad3 inhibitor; inhibits TGF-βR1 signaling Translocation Transcription Factors
Transcriptional Upregulation Others Visit www.novusbio.com, www.rndsystems.com and www.tocris.com to learn more about these genes/proteins and inhibitors. www.novusbio.com Wall Poster
PATHWAYS & KEY MOLECULAR TARGETS
TGF-β Signaling
CANONICAL NON-CANONICAL Differential / Context Dependent TGF-β - SMADs Signaling in Normal and Cancer Cells PATHWAY TGF-β TGF-β PATHWAY TGF-β R I/ NORMAL CELL CANCER CELL Latent TGF-β ALK-5 In normal cells, the activated SMADs translocate into the cell’s nucleus wherein they drive antitumor In cancer cells, p53, PTEN or SMAD4 loss (dotted shapes) favors different signaling mechanisms which effects by inhibiting cell proliferation and inducing apoptosis. drive cell proliferation, apoptotic resistance and EMT/metastasis. TGF-β TGF-β R I/ALK-5 Plasmin, TSP TGF-β Receptors TGF-β R II TGF-β Complex TGF-β Formation TGF-β R II Ligand
P P P P Tyrosine P TGF-β R I/ALK-5 TGF-β R II TGF-β R II Kinase LTBP P P TGF-β R I/ALK-5 TGF-β P Receptor TGF-β R II Plasma Membrane Plasma Membrane SEMA TGF-β P SMURF1/2 TAK1 P P P P SMAD-2/3 P PTEN P P IKK-α RAS P IKK- TGF-β R III β PI3K P IKK-γ RhoA P X SMAD6/7 P SEMA SEMA P I-SMADs BRAF TAK1 RAF P SMAD-3 IkB SMAD-3 PTEN AKT P ALK-1 RAS ROCK CDK PDK1 SMAD-3 SMAD-4 P SMAD-2/3 P P co-SMAD R-SMADs P MAPK SMURF1/2 RelA/p65 p50 SMAD-3 Endoglin SMAD-4 P IkB JNK p38 ERK P P LIMK X TGF-β R II P P SMAD-4 SMAD-3 P P PCB1 Wnt-β-catenin signaling SMAD-4 SMAD-1/5/8 AKT CFL1 P P P P SMAD-3 R-SMADs IkB Degradation SMAD-4 SMURF1/2 mTOR Cytoplasm Cytoplasm Nucleus P P Nucleus SMAD-3 DROSHA SMAD-1/5/8 P p21 SOX4 PTHrP P SMAD-2/3 P Inhibition RelA/p65 Cellular Growth, p50 GTPases of Cell Induction SOX4 Resistance to Signaling p15 Growth KLF5 of Apoptosis EMT & Cancer Metastasis SMAD-4 Apoptosis, S6K via pre-miRNA Processing P EMT/Metastatic P P P SMAD-3 P P Progression PCB1 CBP SMAD-3 SMAD-3 SMAD-4 AKT CBP EMT & Metastasis via p53 Signaling SMAD-4 TF* CBP Alternative Splicing SMAD-4 KLF5 SMAD-3 P p53 P SMAD-1/5/8 P TGF-β Target Genes Target Genes of TGF-β Signaling Small Molecule Inhibitors of TGF-β Signaling SMAD4 Degradation NFkB Processes Target Genes Small Molecule Description Signaling P P SMAD-2/3 RelA/p65 p50 CTGF, FN1, ID1, IL6, MMP2, MMP9, SERPINE1/PAI1, LY 364947 Selective inhibitor of TGF-βRI TF* Angiogenesis CBP SMAD-4 CBP TSP, VEGFA BCL2, BCL2L1/BCL-XL, BIK, BCL2L11/BIM, EIF2A, SB 525334 Selective inhibitor of TGF-βRI TGF-β JNK p38 ERK Apoptosis FAS, ERN1, EIF2AK3/PERK, MAPK8/JNK, SNAI1/Snail, Target SB 505124 Selective inhibitor of TGF-βRI, ALK4 and ALK7 Genes SOX4
SMAD-4 TF* AMPK, AKT, ATG5, ATG7, BECN1, DAPK1, mTOR, TAB1, GW 788388 Potent and selective inhibitor of TGF-βRI P Autophagy c-Jun c-Fos c-Myc TAK1 SMAD-1/5/8 CBP SD 208 Potent ATP-competitive TGF-βRI inhibitor CBP ATF2 4EBP1/EIF4EBP1, AKAP12, ID1, ID2, MYC, p15/ *TF: AP1, RUNX, FOX, bHLH, SP1, IRF7 Cell Proliferation INK4B/ CDKN2B, p21/CIP1/CDKN1A, p57/KIP2/ R 268712 Potent and selective inhibitor of TGF-βRI CDKN1C, PDGFB, PI3K Nucleus Epithelial to ACTN1, CFL1/CFL, COL4A1, HMGCA, MMP2, MMP9, IN 1130 Potent and selective inhibitor of TGF-βRI Mesenchymal NET1, SNAI1/Snail, SNAI2/Slug, TWIST, ZEB1, ZEB2 Cytoplasm Plasma Membrane Transition SB 431542 Potent, selective inhibitor of TGF-βRI, ALK4 and ALK7 Pathway Key: Shape Colors Pathway Key: Connectors Extra-Cellular Matrix ACTA2/α-SMA, COL1A1, COL4A1, CTGF, FN1, LAMC2, A 83-01 Selective inhibitor of TGF-βRI, ALK4 and ALK7 (ECM) Synthesis SPOK1 Direct Activation Ligands FASL, FOXP3, GZMA, GZMB, IFNG/IFG, IgA, IL11, IL17, RepSox Potent and selective inhibitor of TGF-βRI Immune Response Direct Inhibition Receptors IL2, IL6, KLRK1/NKG2D, PRF1, TNFA ITD 1 Selective inhibitor of TGF-β signaling Multi-step Regulation Kinases Self-Renewal/ CTNNB1, HGF, KLF4, LIF, MET, NANOG, SOX2, SOX4, Stemness WNT5A SIS3 Selective Smad3 inhibitor; inhibits TGF-βR1 signaling Translocation Transcription Factors
Transcriptional Upregulation Others Visit www.novusbio.com, www.rndsystems.com and www.tocris.com to learn more about these genes/proteins and inhibitors. www.novusbio.com