PLOS NEGLECTED TROPICAL DISEASES RESEARCH ARTICLE Molecular epidemiology of dengue fever outbreaks in Bhutan, 2016-2017 1 1 1 1 Sangay ZangmoID *, Jit Bdr DarnalID , TshetenID , Sonam GyeltshenID , Binay 1 2 2 Thapa ThapaID , Prinyada Rodpradit , Piyawan Chinnawirotpisan , 2 2 2 1 Wudtichai Manasatienkij , Louis R. MacareoID , Stefan Fernandez , Sonam Wangchuk , Chonticha Klungthong2* 1 Royal Centre for Disease Control, Ministry of Health, Thimphu, Bhutan, 2 Department of Virology, Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand *
[email protected] (SZ);
[email protected] (CK) a1111111111 Abstract a1111111111 a1111111111 Dengue continues to pose a significant public health problem in tropical and subtropical a1111111111 a1111111111 countries. In Bhutan, first outbreak of dengue fever (DF) was reported in 2004 in a southern border town, followed by sporadic cases over the years. In this study, we analysed DF out- breaks that occurred in 3 different places during the years 2016 and 2017. A total of 533 cases in 2016 and 163 in 2017 were suspected of having of DF, where young adults were mostly affected. A total of 240 acute serum specimens collected and analyzed for serotype OPEN ACCESS by nested RT-PCR revealed predominance of serotypes 1 and 2 (DENV-1 and 2). Phyloge- Citation: Zangmo S, Darnal JB, Tsheten , netic analysis using envelope gene for both the serotypes demonstrated cosmopolitan Gyeltshen S, Thapa BT, Rodpradit P, et al. (2020) Molecular epidemiology of dengue fever outbreaks genotype which were closely related to strains from India, indicating that they were probably in Bhutan, 2016-2017. PLoS Negl Trop Dis 14(4): imported from the neighboring country over the past few years.