Monoclonal Antibody to Myosin-1 / MYH1 (Heavy Chain) - Purified

OriGene Technologies, Inc. OriGene Technologies GmbH

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AM05490PU-N Monoclonal Antibody to Myosin-1 / MYH1 (heavy chain) - Purified

Alternate names: Myosin heavy chain 1, Myosin heavy chain 2x, Myosin heavy chain IIx/d, Myosin heavy chain skeletal muscle Quantity: 0.2 mg Concentration: 1.0 mg/ml Background: Myosin II is the so called "classical" myosin, being the first type of myosin to be discovered. Myosin II forms bipolar filaments that interact with actin filaments to produce contraction. Myosin II was first isolated from muscle but is also found in non muscle cells, and it is especially enriched in highly motile cell types such as amoebae. The myosin II molecule is composed of two heavy chains and two sets of light chains. The light chains are the Essential Light Chains (ELC) and Regulatory Light Chains (RLC). Overall activity and polymerization can be modulated by phosphorylation on the light chains and the C terminus of the heavy chains respectively. Muscle myosin is a hexameric protein that consists of two heavy chain subunits (MHC), two alkali light chain subunits (MLC) and two regulatory light chain subunits (MLC-2). Uniprot ID: P02562 NCBI: 9986 Host / Isotype: Mouse / IgG1 Recommended Isotype SM10P (for use in human samples), AM03095PU-N Controls: Clone: BGN/04/4471 Immunogen: Native Myosin heavy chain from muscle. Format: State: Liquid purified IgG fraction Purification: Affinity Chromatography on Protein A Buffer System: PBS Preservatives: 0.09% Sodium Azide Applications: ELISA. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. Specificity: This antibody is specific for Rabbit Myosin heavy chain and shows minimal reactivity with Human cardiac Myosin. Species: Rabbit. Other species not tested. Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch.

For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. MS/20150811 1 / 1