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Regulation of Glycosaminoglycan Synthesis by Thyroid Hormone in Vitro

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Regulation of Glycosaminoglycan Synthesis by Thyroid Hormone in Vitro Regulation of Glycosaminoglycan Synthesis by Thyroid Hormone in Vitro Terry J. Smith, … , Louis Philipson, Samuel Refetoff J Clin Invest. 1982;70(5):1066-1073. https://doi.org/10.1172/JCI110694. Research Article Human skin fibroblasts synthesize and accumulate glycosaminoglycans (GAG). Recently, we reported that fibroblasts incubated in thyroid hormone-deficient media accumulate more GAG than do cultures incubated in the same media enriched with 0.1 μM triiodothyronine (T3) (1981. Endocrinology. 108: 2397). The current study characterizes that enhanced accumulation. Confluent cultures were maintained in thyroid hormone-deficient media without or with added T3, labeled with [3H]acetate and analyzed for total [3H]GAG and [3H]hyaluronic acid content. 3 Addition of T3 to thyroid hormone-depleted media consistently inhibited the incorporation of [H]acetate into GAG by 28- 60% in fibroblast cultures from four different normal human donors. Maximal inhibitory effect was observed within 3 d after hormone addition at concentrations > 1 nM. 73% of the maximal inhibitory effect was observed in the presence of physiologic concentrations of T3 (0.16 nM total T3 or 1.4 pM free T3). 3 The following observations indicated that T3 inhibition of [ H]GAG accumulation is most likely due to a decrease in GAG synthesis rather than to changes in the acetate pool or GAG degradation: (a) Addition of 0, 100, 500, and 2,500 μM unlabeled acetate progressively decreased [3H]acetate incorporation into GAG, up to 80%, without altering the further 3 inhibitory effect of T3 (35-40%); (b). A similar effect of T3 on GAG (32% inhibition) was observed using [ H]glucosamine as substrate; (c) T3 decreased hyaluronate synthetase activity […] Find the latest version: https://jci.me/110694/pdf Regulation of Glycosaminoglycan Synthesis by Thyroid Hormone in Vitro TERRY J. SMITH, YOSHIHARU MURATA, ALLEN L. HORWITZ, Louis PHILIPSON, and SAMUEL REFETOFF, Thyroid Study Unit, Department of Medicine and the Department of Pediatrics, University of Chicago, Illinois 60637 A B S T R A C T Human skin fibroblasts synthesize and effect of T3 on [3H]GAG accumulation appears to be accumulate glycosaminoglycans (GAG). Recently, we quite specific, since the hormone had no effect on the reported that fibroblasts incubated in thyroid hor- incorporation of [3H]leucine into trichloroacetic acid- mone-deficient media accumulate more GAG than do precipitable material. cultures incubated in the same media enriched with Thus, thyroid hormone inhibits GAG accumulation 0.1 ,uM triiodothyronine (T3) (1981. Endocrinology. in a dose-, time-dependent, and reversible manner. 108: 2397). The current study characterizes that en- This inhibition is apparently due to specific effects on hanced accumulation. Confluent cultures were main- the rate of macromolecular synthesis. tained in thyroid hormnone-deficient media without or with added T3, labeled with [3H]acetate and analyzed INTRODUCTION for total [3H]GAG and [3H]hyaluronic acid content. Addition of T3 to thyroid hormone-depleted media Thyroid hormone modulates many aspects of cellular consistently inhibited the incorporation of [3H]acetate metabolism (1, 2). In man, hypothyroidism is char- into GAG by 28-60% in fibroblast cultures from four acterized by a constellation of clinical signs and symp- different normal human donors. Maximal inhibitory toms, including growth failure and mental retardation effect was observed within 3 d after hormone addition (3). In severe, long-standing hypothyroidism, there is at concentrations > 1 nM. 73% of the maximal inhib- a tendency for the deposition of mucinous material in itory effect was observed in the presence of physio- skin, resulting in a characteristic nonpitting edema; logic concentrations of T3 (0.16 nM total T3 or 1.4 pM This material is composed of acid mucopolysaccha- free T3). rides (glycosaminoglycans, GAG),' complex polysac- The following observations indicated that T3 inhi- charides normally found in small amounts (4). bition of [3H]GAG accumulation is most likely due to Human skin fibroblasts offer the experimentalist an a decrease in GAG synthesis rather than to changes in easily accessible tissue that can be propagated in cul- the acetate pool or GAG degradation: (a) Addition of ture and retain many of the phenotypic expressions 0, 100, 500, and 2,500,uM unlabeled acetate progres- typical of differentiated cells. Several biological as- sively decreased [3H]acetate incorporation into GAG, pects of the skin fibroblast have been studied in recent up to 80%, without altering the further inhibitory ef- years. They are known to retain hormone receptors for fect of T3 (35-40%); (b). A similar effect of T3 on GAG triiodothyronine, dexamethasone, insulin, and andro- (32% inhibition) was observed using [3H]glucosamine gen (5-8). Responses to several hormones including as substrate; (c) T3 decreased hyaluronate synthetase glucocorticoids, prostaglandins, cathecholamines, and activity by 32%; and (d) There was no effect of T3 on insulin have been demonstrated in fibroblasts (9-13). GAG degradation in a pulse-chase experiment. The 'Abbreviations used in this paper: BS, bovine serum; D- Portions of this work were presented at the 63rd Annual FCS, thyroid hormone-depleted FCS; D-FCS + T3, D-FCS Meeting of The Endocrine Society, Cincinnati, OH, 17-19 to which T3 was added; FCS, fetal calf serum; GAG, gly- June 1981. cosaminoglycans; HA, hyaluronic acid; T3, triiodothyronine, Address reprint requests to Dr. Smith. His present address T4, tetraiodothyronine or thyroxine; Tx-BS, BS from thyroid- is The Rockefeller University, New York 10021. ectomized animals; Tx-BS + T3, Tx-BS with added T3; UDP- Received for publication 8 September 1981 and in revised GlcNAc, uridine disphosphate-N-acetyl glucosamine; UDP- form 14 July 1982. GJcUA, uridine diphosphate glucuronic acid. 1066 J. Clin. Invest. © The American Society for Clinical Investigation, Inc. * 0021-9738/82/11/1066/08 $1.00 Volume 70 November 1982 1066-1073 Little is known about the effect of thyroid hormone TABLE I in human skin fibroblasts. Current information can be Thyroid Hormone Concentration of Sera summarized as follows: (a) The two metabolically ac- tive thyroid hormones, thyroxine (T4) and triiodothy- Source of serum Total T4 Total T, Free Ts ronine (T3) are transferred from medium to cells. This isgldl ng/dl nM % pM cellular uptake of hormone is directly pr6portional to the concentration of free T4 and T3 in the medium FCS 14.5 164 2.52 0.083 2.09 (14); (b) As in other tissues, metabolism of thyroid BS 5.1 111 1.71 0.096 1.64 Human serum 8.1 130 2.00 0.100 2.00 hormones by fibroblasts involves a conversion of T4 to D-FCS <0.2 41 0.63 0.085 0.54 T3 (14); (c) Fibroblasts possess high affinity nuclear Tx-BS <0.2 44 0.68 0.060 0.41 binding sites for T3 (5, 15); and (d) Observed biologic effects of thyroid hormone include stimulation of growth (16), glucose utilization (14, 17), lactate pro- by Millipore (0.22 ,m) filtration (Millipore, Corp, Bedford, duction (17), and degradation of low density lipopro- MA) before addition to the culture medium. teins (18). Cell culture. Human skin fibroblasts obtained from sev- Recently, we reported that thyroid hormone inhibits eral subjects by skin punch biopsy, were used between the 6th, and 11th serial passages beyond the initial plating. For the accumulation of GAG in a human skin fibroblast experiments, cultures were grown to confluence in 60-mm culture incubated in a thyroid hormone-deficient me- Diam plastic petri dishes using a modified Dulbecco's me- dium (19). The current report describes subsequent dium with Earle's salts, 50 Mlg/ml ascorbic acid, 1 yg/ml studies in fibroblast cultures from several individuals, ferric nitrate, and enriched with 10% FCS at 37°C in an which were undertaken to determine the atmosphere containing 10% CO2 and a relative humidity of kinetics, 100%. Glutamine 435 ug/ml and kanamycin 100 ,g/ml were specificity, reversibility, and dose dependence of this added. inhibition, as well as to ascertain whether the accu- Experiments were carried out only after cultures had mulation of GAG was the result of a changed rate of reached confluence. Cells were then exposed to media con- synthesis or degradation. taining either normal serum (FCS or BS)' thyroid hormone- depleted serum (D-FCS or Tx-BS) or thyroid hormone-de- pleted serum supplemented with T3 (D-FCS + T3 or Tx-BS METHODS + T3) and further incubated for the indicated periods of time. Materials For [3H]GAG accumulation studies, media were removed 24 h before harvesting and substituted with fresh media con- [3HJAcetic acid (2,000 mCi/mmol sp act) and uridine di- taining the same serum and hormone additives with either phosphate-['4C]glucuronic acid (UDP-[14C]GlucUA) (343 10 ,uCi/ml of [3H]acetate (total 40 jACi/plate) or 5 uCi/ml mCi/mmol sp act), [3H]glucosamine (20,300 mCi/mmol sp [3H]glucosamine (total 20 gCi/plate). Various amounts of act) and L-[3HJleucine (1,110 mCi/mg) were obtained from unlabeled sodium acetate were added to the media in order New England Nuclear, Boston, MA. Streptomyces hyaluron- to examine the influence of the saturable uptake of acetate idase was obtained from Miles Laboratories, Inc., Elkhart, on the T3-mediated changes of [3H]GAG accumulation. IN. All chemicals used were of the highest purity commer- [3H]Leucine (5 uCi/ml) was added to the culture medium cially available. Uridine diphosphate sugars were chromato- 24 h before harvesting in an experiment that examined the graphed on two thin-layer systems to determine purity. effect of T3 on protein synthesis. Finally, in the pulse-chase experiment, 60 uiCi of [3H]acetate were added in 4 ml of medium per plate and the labeling time was shortened to Procedures 12 h. Following three washes of the cell layers with 10 ml serumless medium, 4 ml of the corresponding serum-con- Preparation of thyroid hormone-depleted sera.
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