TRPM7 Antibody Affinity Purified Polyclonal Antibody Catalog # AG1448

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TRPM7 Antibody Affinity purified polyclonal antibody Catalog # AG1448

Specification

TRPM7 Antibody - Product Information

Application WB, IHC, ICC Primary Accession Q96QT4 Reactivity Human, Mouse, Rat Host Rabbit Clonality Polyclonal Calculated MW 212697 Homology Rat, mouse- identical.

TRPM7 Antibody - Additional Information Western blot analysis of GH3 cell line (lanes 1 and 2) and mouse brain (lanes 3 Gene ID and 4) lysates: 54822 1,3. Anti-TRPM7 antibody (#AG1448), (1:200). Other Names 2,4. Anti-TRPM7 antibody, preincubated with Transient receptor potential cation channel the control peptide antigen. subfamily M member 7, Channel-kinase 1, Long transient receptor potential channel 7, LTrpC-7, LTrpC7, TRPM7, CHAK1, LTRPC7

Related products for control experiments Control peptide antigen (supplied with the antibody free of charge).

Target/Specificity Peptide CKRRKKDKTSDGPKLFLTEE, corresponding to amino acidֲ residues 1146-1165 of human TRPM7 (Accession Q96QT4). Intracellular, C-terminus.

Dilution WB~~1:200-1:2000 IHC~~1:50 ICC~~1:100 Expression of TRPM7 in rat pituitary gland Immunohistochemical staining of rat Peptide Confirmation hypophysis paraffin embedded transversal Confirmed by amino acid analysis. sections using Anti-TRPM7 antibody (#AG1448), (1:50). TRPM7 is stained in both Format the posterior part or neurohypophysis (N) and Affinity purified antibody, lyophilized powder the anterior part or adenohypophysis (A). Note that stain is highly specific for andocrine cells Reconstitution in the adenohypophysis. Color reaction was 25 µl, 50 µl or 0.2 ml deionized water, obtained with SuperPicture HRP-conjugated depending on the sample size. polymer (Zymed) followed by DAB. Hematoxilin is used as the Counterstain. Antibody Concentration After Reconstitution 0.8 mg/ml.

Buffer After Reconstitution Phosphate buffered saline (PBS), pH 7.4, 1% BSA, 0.05% NaN3.

Storage Before Reconstitution Lyophilized powder can be stored intact at room temperature for several weeks. For longer periods, it should be stored at -20°C. Expression of TRPM7 in GH3 cell lines Storage After Reconstitution Immunocytochemical staining of GH3 cells with The reconstituted solution can be stored at Anti-TRPM7 antibody (#AG1448), (1:100), 4ºC for up to 2 weeks. For longer periods, followed by goat anti-rabbit-AlexaFluor-550 small aliquots should be stored at -20ºC or secondary antibody (A). B. Nuclear staining of below. Avoid multiple freezing and thawing. GH3 cells with the cell-permeable dye Hoechst The further dilutions should be made using a 33342. C. Live intact GH3 cells. carrier protein such as BSA (1%). Centrifuge all antibody preparations before use (10000 × g 5 min). TRPM7 Antibody - Background Control Antigen Storage Before Reconstitution TRP channels are a large family (about 28 Lyophilized powder can be stored intact at genes) of plasma membrane, non-selective room temperature for several weeks. For cationic channels that are either specifically or longer periods, it should be stored at -20°C. ubiquitously expressed in excitable and non-excitable cells.1 The TRP channels have Control Antigen Reconstitution putative six-transmembrane domains (TM) with a 100 µl water. pore domain between the fifth and the six TM, and all assemble as tetramers. Both the N- and Control Antigen Storage After the C-terminus of all TRPs are intracellular.3 Reconstitution According to IUPHAR the TRP family comprises of -20ºC. three main subfamilies on the basis of sequence homology; TRPC (canonical), TRPV (vanilloid) and Preadsorption Control TRPM (melastatin). To date, three extra 1 µg peptide per 1 µg antibody. subfamilies are also considered to belong to the TRP family; the TRPA, TRPML, and the TRPP.1-4 The TRPM subfamily consists of eight members, TRPM7 Antibody - Protocols TRPM1 to TRPM8, which also can be further subdivided into four subgroups based on their Provided below are standard protocols that you sequence homology: (1)TRPM1 and TRPM3 (2) may find useful for product applications. TRPM6 and TRPM7 (3) TRPM4 and TRPM5 (4) TRPM2 and TRPM8.5 TRPM7 and TRPM6 are 2+ • Western Blot involved in Mg homeostasis and are unique among the TRP family members, possessing a • Blocking Peptides 6,7 • Dot Blot functional kinase domain at their C-terminus. • Immunohistochemistry Although the kinase is not necessary to the function of the channel it may have a role in • Immunofluorescence 7 • Immunoprecipitation modulating the activation of the channel. Recent work demonstrated that TRPM7 is a • Flow Cytomety 7,8 • Cell Culture critical mediator of anoxic cell death. Abgent is pleased to offer a highly specific antibody directed against an intracellular epitope of human TRPM7. The Anti-TRPM7 antibody (#AG1448) can be used in western blot analysis, immunocytochemical and immunohistochemical applications. It has been designed to recognize TRPM7 from human, rat and mouse samples.

TRPM7 Antibody - References

References 1. Montell, C. et al. (2002) Mol. Cell. 9, 229. 2. Clapham, D.E. (2003) Nature 426, 517. 3. Moran, M.M. et al. (2004) Curr.Opin.Neurobiol. 14, 362. 4. Clapham, D.E. et al. (2003) Pharmacol. Rev. 55, 591. 5. Harteneck, C. (2005) Naunyn Schmiedebergs Arch Pharmacol. 371, 307. 6. Schmitz, C. et al. (2005) J.Biol.Chem. 280, 37763. 7. Aarts, M.M. and Tymianski, M. (2005) Neuroscientist 11, 116. 8. Aarts, M.M. and Tymianski, M. (2005) Pflugers Arch – Eur.J.Physiol. 451, 243.