RnDSy-lu-2945 Development

Spleen, Lymph Nodes, Mucosal-Associated Bone Marrow Spleen Lymphoid Tissue, and Bone Marrow

Mouse B1 Mouse B2 T3 B Cell Progenitor Progenitor (human) Peripherial Blood

BCR Igα/β Plasmablast T1 B Cell Activated B Cell Hematopoietic Naive Mature Activated Germinal Pro-B Cell Pre-B Cell Immature B Cell (mouse; from B2 B Cell Marginal Igα/β Center B Cell Stem Cell Pre-Pro B Cell Progenitor Cell) Zone B Cell BCR BCR BCR Short-lived α β BCR Igα/β Germinal Bone Marrow Ig / BCR Igα/β Center Pro-BCR Pre-BCR Igα/β α β B Igα/β Igα/β T2 B Cell Igα/β BCR Ig / Progenitor Peripherial Blood Transitional B1 Cell Long-lived Multipotent Follicular BCR Antigen Follicular Helper Plasma Cell Progenitor Cell (mouse; from B1 B Cell BCR T Cells Progenitor Cell) Igα/β Activated BCR B Cell Memory B Cell Igα/β Peritoneal Cavity B1a B1b

Common Myeloid Common Lymphoid Progenitor Progenitor

Common Activated Stages of Lymphoid B Lymphocyte Mouse B1 Mouse B2 B1a Cell B1b Cell Transitional Mouse T1 B Cell Mouse T2 B Cell Marginal Zone Germinal Center Development Progenitor Progenitor Progenitor Progenitor Pre-Pro B Cell Pro-B Cell Pre-B Cell Immature B Cell (from mouse B1 progenitor cell) B Cell (from mouse B2 progenitor cell) B Cell Follicular B Cell B Cell Memory B Cell Plasmablast Plasma Cell Human Markers CD10/ CD10/Neprilysin CD10/Neprilysin CD10/Neprilysin CD10/Neprilysin CD10/Neprilysin CD5 CD1c CD19 CD19 CD19 BCMA BCMA CD34 CD34 CD34 CD19 CD19 CD19 CD19 CD19 CD20/MS4A1 CD20/MS4A1 CD20/MS4A1 CD27high BLIMP1 Pax5 Pax5 CD38 CD20/MS4A1 CD20/MS4A1 CD20/MS4A1 CD20/MS4A1 CD20/MS4A1 CD21 CD27 CD21 CD38 CD27high Pax5 CD24 CD24 CD21 CD21 CD21 CD22/Siglec-2 CD38 CD27mid/+ C1q R1/CD93 CD38high Positive CD34 CD38 CD24 CD23/Fce RII CD27 CD23/Fce RII CD40 TACI Syndecan-1/ markers CD38 C1q R1/CD93 CD38 CD24 FCRL3/FcRH3 CXCR5 CD83 CD138 C1q R1/CD93 IL-3 R CD40 CD38 TACI MHC class II MHC class II CXCR4 IL-3 R IL-4 Ra C1q R1/CD93 C1q R1/CD93 TACI TACI IL-7 Ra IL-7 Ra IL-4 Ra TACI Pax5 Pax5 CD117/c-kitlow CD117/c-kitlow CD117/c- CD117/c-kit CD10/Neprilysinlow CD10/Neprilysin C1q R1/CD93 CD19low CD19low Negative CD34 CD27 CD27 CD24low Syndecan-1/ CD20/MS4A1-/low markers IL-7 Ra CD27 CD138-/low MHC class IIlow CD38low Mouse Markers IL-7 Ra Ly6D CD19 B220/CD45 R B220/CD45 R B220/CD45 R B220/CD45 R B220/CD45 R CD1dmid CD1dmid B220/CD45 R B220/CD45 R B220/CD45 R B220/CD45 R B220/CD45 R B220/CD45 R CD19 BLIMP1 Flt-3/Flk-2 IL-7 Ra C1q R1/CD93 CD43 CD19 CD19 CD19 CD5 CD19high CD19 CD19 CD1d CD1dmid CD19 CD19 CD27high CD27high Positive Flt-3/Flk-2 C1q R1/CD93 CD24 CD24 CD24 CD19high CD43 CD24 CD24 CD19mid CD19mid CD40 CD21 CD38 CXCR4high markers CXCR4 CD43 IL-7 Ra C1q R1/CD93 CD43 C1q R1/CD93 C1q R1/CD93 CD21high CD23/Fce RII MHC class II CD27mid/+ Syndecan-1/ Syndecan-1/ Flt-3/Flk-2 IL-7 Ra IgM IgM IgM IgMhigh CXCR5 CD40 CD138 CD138 IL-7 Ra IgD IgDhigh MHC class II Lin (CD3, CD4, Lin (CD3, CD4, Lin (CD3, CD4, Lin (CD3, CD4, Lin (CD3, Gr-1/ Lin (CD3, Gr-1/ Lin (CD3, Gr-1/ CD23/Fce RII CD23/Fce RII CD5 CD43 CD43 CD23/Fce RII CD21low B220/CD45 Rlow B220/CD45 Rlow CD8, Gr-1/Ly-6G, CD8, Gr-1/Ly-6G, CD8, Gr-1/Ly-6G, CD8, Gr-1/Ly-6G, Ly-6G, Integrin Ly-6G, Integrin Ly-6G, Integrin CD43 CD23/Fce RII IgDlow CD43 CD43 CD19 Integrin aM/ Integrin aM/ Integrin aM/ Integrin aM/ aM/CD11b, aM/CD11b, aM/CD11b, IgD C1q R1/CD93 IgMlow CD38low Negative CD11b, TER-119) CD11b, TER-119) CD11b, TER-119) CD11b, TER-119) TER-119) TER-119) TER-119) IgDlow MHC class II-/low markers CD117/c-kit CD117/c-kit B220/CD45 Rlow CD19 CD19 CD117/c-kitlow CD43 Sca-1/Ly6 Sca-1/Ly6 C1q R1/CD93 CD24low IgM IgM Ly6D CD117/c-kit IgM © 2015 R&D Systems, Inc.

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