DOCSLIB.ORG

BASOPHIL ACTIVATION TESTING of Adults and Children Do Have a Food Allergy1

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
BASOPHIL ACTIVATION TESTING of Adults and Children Do Have a Food Allergy1 BasophilBasophil ActivationActivation TestingTesting An in vitro test for the diagnosis of IgE and non-IgE mediated allergies Alpha Laboratories Ltd. Diagnostics 2018 A Safer Allergy Testing Alternative for Your Patients A Safer Alternative for Allergy Testing 2 A Proposed Patient Pathway for Allergy 2-3 Introducing Flow CAST® Basophil Activation Test 3 Allergy Testing- Without the Risk 4 Case Study - University Hospital Southampton Diagnosing Drug Allergy: A Rational Approach 5-6 Expert Opinion - Dr Cathy van Rooyen BÜHLMANN Allergens List 7-8 A Safer Alternative for Allergy Testing The physical symptoms of allergy can be similar BAT is a safer alternative to a provocation to those of a sensitivity or intolerance. Roughly challenge for your patient. Offering BAT before 20% of the population experience symptoms a challenge test will significantly reduce the that make them believe they have a food allergy. number of patients having to undertake a However, it is estimated that actually only 1-10% provocation challenge. For example a peanut BASOPHIL ACTIVATION TESTING of adults and children do have a food allergy1. study undertaken by Santos et al. has shown a 67% reduction in the need for oral food 3 INTRODUCTION Less than 10% of people who think they are challenge by using BAT as the primary test. allergic to penicillin are truly allergic. If someone Eliminating the need for a provocation challenge For over 40 years Alpha Laboratories is labelled as penicillin allergic they are likely to improves patient safety and comfort. be treated with broad-spectrum antibiotics2. An has been providing quality supply incorrect label of penicillin allergy may lead to The true value of BAT lies in its high specificity, and support to UK and international inappropriate use of broad-spectrum antibiotics, (reaching 100% in some studies3). A positive laboratory and clinical scientists. This increasing the threat of antibiotic resistance2. BAT result confirms allergy with a high degree family run company is led by its vision of certainty, resulting in fewer of the more to “find new ways to help science It is vital to distinguish which patients are dangerous positive provocation challenges being improve people’s lives through its truly allergic from those who are not. Basophil performed. evolving range of specialist Diagnostic Activation Testing (BAT) can be a helpful and Laboratory Products.” diagnostic tool to achieve this. References 1. Allergy UK. Allergy Prevalence: Useful facts and Studies have shown that BAT has a higher figures. Through worldwide connections sensitivity and specificity than standard skin prick 2. NICE Clinical Guideline 183. Drug Allergy: Diagnosis and relationships with leading tests (SPT) and specific IgE tests (sIgE), making and management of drug allergy in adults, children and young people. September 2014. manufacturers, established and it a useful second line test if SPT and sIgE results emerging suppliers, Alpha Laboratories 3. Santos AF, Shreffler WG. Road map for the clinical are unable to offer a confirmed result. application of the basophil activation test in food sources the best products to help allergy. Clin Exp Allergy. 2017; 47:1115–1124. https:// solve the problems you face in your doi.org/10.1111/cea.12964 laboratory or clinic every day. Bringing you solutions that: ■ Improve the quality of your results A Proposed Patient Pathway for Allergy ■ Save time ■ Lower overall costs ■ Maximise your uptime & efficiency Clinical History ■ Improve your supply chain ■ Reduce health and safety risks ■ Support better patient outcomes in Skin Prick Test (SPT) clinical environments and/or Specific IgE Test (sIgE) Our partnership with BÜHLMANN Laboratories AG extends over 10 years and we are proud to be their exclusive Negative UK distributor. BÜHLMANN is an Allergy confirmed Positive Results if clinical history Allergy excluded is convincing innovative specialist in vitro diagnostics manufacturer with a selection of unique, high-quality assays for routine Equivocal clinical use. Allergy is one area of focus for Basophil Activation BÜHLMANN and Alpha Laboartories. Test (BAT) Where specific IgE fails to demonstrate absolute diagnostic reliability, especially in more complex reactions such as drug, venom or food allergies we have Allergy confirmed Positive Results Negative the solution - The complete Cellular Allergy diagnostics package. Provocation Challenge Cover image background photo by Joel Filipe on Unsplash.com Allergy confirmed Positive Results Negative Allergy excluded 2 Introducing Flow CAST® Basophil Activation Testing Made Easy The Flow CAST® system, manufactured by Upon response to a purified allergen which Flow CAST® Data Acquisition BÜHLMANN Laboratories, represents a is added to the sample, sensitised basophils significant step forward in in vitro allergy degranulate and express the cell surface marker testing. Using a unique dual marker CD63. This is detected by a FITC-fluorescence combination (CCR3 and CD63) it allows the labelled monoclonal antibody. CD63 is currently simple detection of Basophil Activation on the best-validated basophil activation marker whole blood samples via flow cytometry. Flow directly linked to basophil degranulation. CAST BAT can be used to identify immediate The Flow CAST kit can identify both IgE and type allergic reactions and hypersensitivities to non-IgE mediated allergies, making it ideal suspected allergens. for determining allergies to anaesthetics, antibiotics and NSAIDs. Basophils constitutively express the eotaxin receptor CCR3 allowing for a robust and accurate selection of cells with flow cytometry. The Flow CAST kit is quick and easy to use, CCR3 is detected with a PE-fluorescence with results obtained in ~60 minutes. Samples labelled monoclonal antibody. This negates the can be analysed up to 48 hours after collection Figure 1: Three discrete populations; requirement for lengthy and labour intensive (up to 24 hours for drug responses). The lymphocytes, monocytes and leukocyte isolation steps. kit contains all of the reagents and controls granulocytes in FSC/SSC histogram. needed to run the test. An extensive range of over 150 standardised allergens spanning food, drug, insect venoms, inhalants and food additive allergies are available to purchase separately. Find out more about Flow CAST kits (Product Code FK-CCR) and purchase online at ww.alphalabs.co.uk/flowcast Figure 2: Selection of basophilic cells CCR3pos / SSClow Clinical History Skin Prick Test (SPT) and/or Specific IgE Test (sIgE) Negative Allergy confirmed Positive Results if clinical history Allergy excluded is convincing Equivocal Figure 3: Patient Background (PB) with Stimulation Buffer only Basophil Activation Test (BAT) Allergy confirmed Positive Results Negative Provocation Challenge Allergy confirmed Positive Results Negative Allergy excluded Figure 4: Positive Control (PC) with Stimulation control anti-FceRI Ab Basophil Activation Testing Allergy Testing- Without the Risk Yoon Tak Chin, Bryan Fernandes, Adnan Mani, Clive McLean, Efrem Eren. University Hospital Southampton NHS Foundation Trust, Laboratory medicine, Division of Diagnostics and Therapy Department of Immunology. In some cases of allergens that cause Ordinarily, drug allergy testing to ibuprofen immediate complex reactions, such as would involve a drug provocation those to drug allergens, specific IgE fails challenge while drug allergy testing to penicillin to give absolute diagnostic reliability. would involve a series of skin prick Whilst drug provocation tests remain the and intradermal tests. gold standard for diagnosis of immediate drug allergies, they are very time intensive However, in view of the severity of the reaction, procedures associated with the significant the young girl, her mother and our Paediatric risk of severe and potentially fatal colleagues were understandably reluctant to reactions. Likewise, skin prick and intra- perform these tests. dermal testing are slow and risk severe reactions. In vitro tests such as specific Instead a blood sample was taken and tested IgE to drugs offer a limited range of drugs using the BÜHLMANN Flow CAST® BAT kits. that can be tested. Results for ibuprofen and amoxicillin were both Basophil Activation Tests (BAT) provide an positive. BAT was negative for other agents References 1. Ebo DG, Bridts CH, Hagendorens MM, Mertens CH, alternative test that does not carry risks to including aspirin, penicillin major determinants De Clerck LS, Stevens WJ. Flow-assisted diagnostic the patient. They have emerging potential and penicillin minor determinants. management of anaphylaxis from rocuronium in assisting in the clinical work-up and bromide. Allergy. 2006;61:935-9. diagnosis of immediate drug allergies. This data helped define the Paediatric Team’s 2. Gamboa PM, Sanz ML, Caballero MR, Antépara I, management plan, without putting the young Urrutia I, Jáuregui I, González G, Diéguez I, De Weck Here a team from the Department girl through a long procedure or the risk of a AL. Use of CD63 expression as a marker of in vitro of Immunology, University Hospital severe reaction. basophilactivation and leukotriene determination Southampton NHS Foundation Trust, in metamizol allergic patients. Allergy. 2003;58:312-7. 3. Gómez E, Blanca-López N, Torres MJ, Requena describe an example of their experience There are studies showing that BAT can be G, Rondon C, Canto G, Blanca M, Mayorga C. using BAT in a clinical setting. helpful in diagnosing immediate allergy to some Immunoglobulin E-mediated immediate
Load more

Recommended publications
  • Mouse and Human Fcr Effector Functions
    Pierre Bruhns Mouse and human FcR effector € Friederike Jonsson functions Authors’ addresses Summary: Mouse and human FcRs have been a major focus of Pierre Bruhns1,2, Friederike J€onsson1,2 attention not only of the scientific community, through the cloning 1Unite des Anticorps en Therapie et Pathologie, and characterization of novel receptors, and of the medical commu- Departement d’Immunologie, Institut Pasteur, Paris, nity, through the identification of polymorphisms and linkage to France. disease but also of the pharmaceutical community, through the iden- 2INSERM, U760, Paris, France. tification of FcRs as targets for therapy or engineering of Fc domains for the generation of enhanced therapeutic antibodies. The Correspondence to: availability of knockout mouse lines for every single mouse FcR, of Pierre Bruhns multiple or cell-specific—‘a la carte’—FcR knockouts and the Unite des Anticorps en Therapie et Pathologie increasing generation of hFcR transgenics enable powerful in vivo Departement d’Immunologie approaches for the study of mouse and human FcR biology. Institut Pasteur This review will present the landscape of the current FcR family, 25 rue du Docteur Roux their effector functions and the in vivo models at hand to study 75015 Paris, France them. These in vivo models were recently instrumental in re-defining Tel.: +33145688629 the properties and effector functions of FcRs that had been over- e-mail: [email protected] looked or discarded from previous analyses. A particular focus will be made on the (mis)concepts on the role of high-affinity Acknowledgements IgG receptors in vivo and on results from antibody engineering We thank our colleagues for advice: Ulrich Blank & Renato to enhance or abrogate antibody effector functions mediated by Monteiro (FacultedeMedecine Site X.
    [Show full text]
  • Basophil Activation Receptors Inhibit Mouse and Human Γ Fc
    Fcγ Receptors Inhibit Mouse and Human Basophil Activation Lydie Cassard, Friederike Jönsson, Ségolène Arnaud and Marc Daëron This information is current as of September 24, 2021. J Immunol published online 20 August 2012 http://www.jimmunol.org/content/early/2012/08/20/jimmun ol.1200968 Downloaded from Supplementary http://www.jimmunol.org/content/suppl/2012/08/20/jimmunol.120096 Material 8.DC1 Why The JI? Submit online. http://www.jimmunol.org/ • Rapid Reviews! 30 days* from submission to initial decision • No Triage! Every submission reviewed by practicing scientists • Fast Publication! 4 weeks from acceptance to publication *average by guest on September 24, 2021 Subscription Information about subscribing to The Journal of Immunology is online at: http://jimmunol.org/subscription Permissions Submit copyright permission requests at: http://www.aai.org/About/Publications/JI/copyright.html Email Alerts Receive free email-alerts when new articles cite this article. Sign up at: http://jimmunol.org/alerts The Journal of Immunology is published twice each month by The American Association of Immunologists, Inc., 1451 Rockville Pike, Suite 650, Rockville, MD 20852 Copyright © 2012 by The American Association of Immunologists, Inc. All rights reserved. Print ISSN: 0022-1767 Online ISSN: 1550-6606. Published August 20, 2012, doi:10.4049/jimmunol.1200968 The Journal of Immunology Fcg Receptors Inhibit Mouse and Human Basophil Activation Lydie Cassard,*,†,1 Friederike Jo¨nsson,*,†,1 Se´gole`ne Arnaud,*,† and Marc Dae¨ron*,† Besides high-affinity IgE receptors (Fc«RI), human basophils express activating (FcgRIIA) and inhibitory (FcgRIIB) low-affinity IgG receptors. IgG receptors (FcgR) were also found on mouse basophils, but not identified.
    [Show full text]
  • Food Allergy and Food Intolerance: Specific Versus Innate Immunity Roger Davis Deutsch
    The Original Internist article Food Allergy and Food Intolerance: specific versus innate immunity Roger Davis Deutsch Nomenclature The concept of an allergy was fairly well understood when von Pirquet, an Austrian physician, first introduced the term in 1906. It was intended to denote an altered reaction to a normally innocuous substance. Two decades later allergists in Europe agreed to narrow the definition to apply only to such an altered reaction as may be characterized by an immediate onset of symptoms. Contemporary physicians recognize a distinction between immediate and delayed-type altered reactions in their nomenclature by applying the terms, “intolerance” or “sensitivity” in the case of common reactions to foods and other chemicals, where symptom onset is delayed and typically of a less acute nature than that which has come to be known as a “true” allergy, classical allergy, or Type I hypersensitivity, according to the Gell and Coombs classification system. Although both phenomena involve an aberrant immune response, the mechanisms underlying each are quite different. Broadly speaking, classical allergy is a function of the specific immune system, whilst intolerance involves activation of the innate branch of the immune system. Classical allergy is a function of the specific immune system The most prominent hallmark of specific immunity is memory; hence the rationale underlying immunization. It is termed, “specific” because it makes use of pathogen- or allergen-specific binding sites on Immunoglobulins or lymphocyte receptor molecules in the process of recognition. Prior exposure to the activating substance is therefore necessary in order to “prime” or elicit a subsequent response of the specific immune system.
    [Show full text]
  • Decreased Intracellular Histamine Concentration and Basophil Activation in Anaphylaxis
    Allergology International 69 (2020) 78e83 Contents lists available at ScienceDirect Allergology International journal homepage: http://www.elsevier.com/locate/alit Original Article Decreased intracellular histamine concentration and basophil activation in anaphylaxis Satoshi Yamaga a, Yuhki Yanase b, Kaori Ishii b, Shinichiro Ohshimo a, Nobuaki Shime a, * Michihiro Hide b, a Department of Emergency and Critical Care Medicine, Graduate School of Biomedical & Health Sciences, Hiroshima University, Hiroshima, Japan b Department of Dermatology, Graduate School of Biomedical & Health Sciences, Hiroshima University, Hiroshima, Japan article info abstract Article history: Background: Histamine is a crucial mediator in the development of anaphylaxis. Although histamine is Received 9 February 2019 promptly degraded because of its short half-life in plasma, basophils, which release histamine, remain in Received in revised form the blood for days. To explore basophils as a potential marker and their involvement in the pathogenesis 18 May 2019 of anaphylaxis, we evaluated the intracellular histamine concentration and the degree of basophil Accepted 21 May 2019 activation in anaphylaxis patients. Available online 24 June 2019 Methods: We conducted a caseecontrol study enrolling anaphylaxis patients and healthy controls. Basophil activation was evaluated by flow cytometry using up-regulation of CD203c expression. Keywords: Anaphylaxis Results: We enrolled 23 patients and measured their blood histamine concentration. Basophil activation Basophil activation was analyzed in seven of 23 patients. The median intracellular histamine concentrations at admission CD203c were significantly lower in patients compared with controls (16.4 ng/mL [interquartile range {IQR}, 2.70 Intracellular histamine to 34.0] vs. 62.3 ng/mL [IQR, 46.0 to 85.1]; p < 0.0001).
    [Show full text]
  • Road Map for the Clinical Application of the Basophil Activation Test in Food Allergy
    Road map for the clinical application of the basophil activation test in food allergy The Harvard community has made this article openly available. Please share how this access benefits you. Your story matters Citation Santos, A. F., and W. G. Shreffler. 2017. “Road map for the clinical application of the basophil activation test in food allergy.” Clinical and Experimental Allergy 47 (9): 1115-1124. doi:10.1111/cea.12964. http://dx.doi.org/10.1111/cea.12964. Published Version doi:10.1111/cea.12964 Citable link http://nrs.harvard.edu/urn-3:HUL.InstRepos:34492249 Terms of Use This article was downloaded from Harvard University’s DASH repository, and is made available under the terms and conditions applicable to Other Posted Material, as set forth at http:// nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of- use#LAA DOI: 10.1111/cea.12964 REVIEW Road map for the clinical application of the basophil activation test in food allergy A. F. Santos1 | W. G. Shreffler2 1Department of Paediatric Allergy, King’s College London/Guy’s and St Thomas’ Summary Hospital, London, UK The diagnosis of IgE-mediated food allergy based solely on the clinical history and the 2Department of Pediatrics, Division of documentation of specific IgE to whole allergen extract or single allergens is often Allergy and Immunology, Food Allergy Center, Massachusetts General Hospital, ambiguous, requiring oral food challenges (OFCs), with the attendant risk and inconve- Harvard Medical School, Boston, MA, USA nience to the patient, to confirm the diagnosis of food allergy. This is a considerable Correspondence proportion of patients assessed in allergy clinics.
    [Show full text]
  • Basophils from Cancer Patients Respond to Immune Stimuli and Predict Clinical Outcome
    cells Article Basophils from Cancer Patients Respond to Immune Stimuli and Predict Clinical Outcome Heather J. Bax 1,2 , Jitesh Chauhan 1,2, Chara Stavraka 1,2,3 , Atousa Khiabany 1,2, Mano Nakamura 1, Giulia Pellizzari 1, Kristina M. Ilieva 1,4, Sara Lombardi 5, Hannah J. Gould 6,7, Christopher J. Corrigan 7,8, Stephen J. Till 7,8, Sidath Katugampola 9 , Paul S. Jones 9, Claire Barton 9,10, Anna Winship 3, Sharmistha Ghosh 3, Ana Montes 3, Debra H. Josephs 1,2,3, James F. Spicer 2,3 and Sophia N. Karagiannis 1,4,* 1 St. John’s Institute of Dermatology, School of Basic & Medical Biosciences, King’s College London, London SE1 9RT, UK; [email protected] (H.J.B.); [email protected] (J.C.); [email protected] (C.S.); [email protected] (A.K.); [email protected] (M.N.); [email protected] (G.P.); [email protected] (K.M.I.); [email protected] (D.H.J.) 2 School of Cancer & Pharmaceutical Sciences, King’s College London, Guy’s Hospital, London SE1 9RT, UK; [email protected] 3 Departments of Medical Oncology and Clinical Oncology, Guy’s and St Thomas’ NHS Foundation Trust, London SE1 9RT, UK; [email protected] (A.W.); [email protected] (S.G.); [email protected] (A.M.) 4 Breast Cancer Now Research Unit, School of Cancer & Pharmaceutical Sciences, King’s College London, Guy’s Cancer Centre, London SE1 9RT, UK 5 Guy’s and St Thomas’ Oncology & Haematology Clinical Trials (OHCT), Guy’s Cancer Centre, London SE1 9RT, UK; [email protected] 6 Randall Centre for
    [Show full text]
  • Measurement of Mast Cell and Basophil Activation in Vitro As Means for Investigation of Drug Hypersensitivity
    University of Southampton Faculty of Medicine Measurement of mast cell and basophil activation in vitro as means for investigation of drug hypersensitivity by Desiree Ludwig Thesis of the degree of Master of Philosophy May 2015 University of Southampton, Faculty of Medicine, Master of Philosophy, Desiree Ludwig Measurement of mast cell and basophil activation in vitro as means for investigation of drug hypersensitivity Abstract Allergic drug reactions to drugs are becoming more frequent throughout the world and are among the most common and serious form of immuno-pathological process in modern clinical medicine. These reactions can lead to life-threatening anaphylaxis which can occur within minutes of receiving the drug. Symptoms provoked by the explosive release of mediators from mast cells and basophils release may include itchiness, angioedema, difficulty breathing and circulatory collapse. There are few reliable laboratory tests available to identify those with drug hypersensitivity who may be at risk of anaphylactic shock, or even to establish if such a reaction has occurred. Confirmation of drug hypersensitivity by skin testing or oral challenge can put the patient at risk of serious reaction, and as such are expensive and performed at few centres. The aim of the study was to seek to develop new tests for drug allergy based on assessment of mast cell or basophil activation either in vitro or in vivo. Patients were recruited who were suspected of having suffered an allergic reaction to medicine and who were undergoing skin testing or oral drug challenge. Blood was collected before and following the challenge, the nature of symptoms provoked were recorded, and clinical history and demographic data obtained.
    [Show full text]
  • Biomarkers of the Involvement of Mast Cells, Basophils and Eosinophils in Asthma and Allergic Diseases
    Biomarkers of the involvement of mast cells, basophils and eosinophils in asthma and allergic diseases The Harvard community has made this article openly available. Please share how this access benefits you. Your story matters Citation Metcalfe, D. D., R. Pawankar, S. J. Ackerman, C. Akin, F. Clayton, F. H. Falcone, G. J. Gleich, et al. 2016. “Biomarkers of the involvement of mast cells, basophils and eosinophils in asthma and allergic diseases.” The World Allergy Organization Journal 9 (1): 7. doi:10.1186/s40413-016-0094-3. http://dx.doi.org/10.1186/ s40413-016-0094-3. Published Version doi:10.1186/s40413-016-0094-3 Citable link http://nrs.harvard.edu/urn-3:HUL.InstRepos:25658402 Terms of Use This article was downloaded from Harvard University’s DASH repository, and is made available under the terms and conditions applicable to Other Posted Material, as set forth at http:// nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of- use#LAA Metcalfe et al. World Allergy Organization Journal (2016) 9:7 DOI 10.1186/s40413-016-0094-3 REVIEW Open Access Biomarkers of the involvement of mast cells, basophils and eosinophils in asthma and allergic diseases Dean D. Metcalfe1*, Ruby Pawankar2, Steven J. Ackerman3, Cem Akin4, Frederic Clayton5, Franco H. Falcone6, Gerald J. Gleich7, Anne-Marie Irani8, Mats W. Johansson9, Amy D. Klion10, Kristin M. Leiferman11, Francesca Levi-Schaffer12, Gunnar Nilsson13, Yoshimichi Okayama14, Calman Prussin1, John T. Schroeder15, Lawrence B. Schwartz16, Hans-Uwe Simon17, Andrew F. Walls18 and Massimo Triggiani19 Abstract Biomarkers of disease activity have come into wide use in the study of mechanisms of human disease and in clinical medicine to both diagnose and predict disease course; as well as to monitor response to therapeutic intervention.
    [Show full text]
  • Improved Diagnosis and Therapy of Hymenoptera Venom Allergy by Component-Resolved Approaches
    Fakultät für Medizin Lehrstuhl für molekulare Allergologie Improved Diagnosis and Therapy of Hymenoptera Venom Allergy by Component-Resolved Approaches Maximilian Schiener Vollständiger Abdruck der von der Fakultät für Medizin der Technischen Universität München zur Erlangung des akademischen Grades eines Doktors der Naturwissenschaften (Dr. rer. nat.) genehmigten Dissertation. Vorsitzende: Prof. Dr. Christina Zielinski Prüfende/-r der Dissertation: 1. Priv.-Doz. Dr. Simon Blank 2. Prof. Dr. Michael Sattler Die Dissertation wurde am 23.01.2018 bei der Technischen Universität München eingereicht und durch die Fakultät für Medizin am 04.07.2018 angenommen. Abstract Patients suffering from allergies due to stings of Hymenoptera like Apis mellifera, Vespula vulgaris and Polistes dominula can experience severe to fatal anaphylactic reactions. Be- sides emergency treatment, venom immunotherapy (VIT) is the only curative treatment. VIT products are available from different manufactures and treatment efficacy is very high compared to other forms of allergen-specific immunotherapy. Nevertheless, thera- peutic failures can be observed and possibly originate from different venom extraction or work-up techniques. To reduce the cost resulting from unnecessary treatment with more than one venom and to avoid the chance of de novo sensitization, the identification of the insect causing the systemic reaction has to be unambiguous. Not all patients can correctly identify the causative insect and, moreover, diagnosis by skin testing is not al- ways straightforward. Therefore, serum specific immunoglobulin E (sIgE) levels to crude insect venom can be determined in routine diagnosis. In the past, this was shown to have several disadvantages, as single allergens can be underrepresented in venom extracts due to low biologic availability or stability, thus patients sensitized to those allergens might be missed.
    [Show full text]
  • Ige in the Pathogenesis of SLE: from Pathogenic Role to Therapeutic Target
    antibodies Review IgE in the Pathogenesis of SLE: From Pathogenic Role to Therapeutic Target Yasmine Lamri 1,2 and Nicolas Charles 1,2,* 1 Centre de Recherche sur l’Inflammation, CNRS ERL8252 Faculté de Médecine Site Bichat, Université de Paris, INSERM UMR1149, 16 Rue Henri Huchard, F-75018 Paris, France; [email protected] 2 Laboratoire d’Excellence INFLAMEX, Université de Paris, F-75018 Paris, France * Correspondence: [email protected] Received: 22 October 2020; Accepted: 22 November 2020; Published: 8 December 2020 Abstract: Systemic lupus erythematosus (SLE) is a multifactorial chronic autoimmune disease, marked by the presence of autoantibodies to nuclear antigens belonging to different isotype classes. For several years, IgE antibodies have been incriminated in the development of allergic diseases and parasitic infections and different anti-IgE therapies have been developed to encounter the pathogenic role of IgE in these pathologies. Recently, multiple studies showed the presence of elevated total IgE levels and demonstrated a pathogenic role of autoreactive IgE in SLE. This review aims to summarize the findings incriminating IgE and autoreactive IgE in the pathophysiology of SLE, to describe their functional outcomes on their targeted cells as well as to discuss different IgE-related therapeutic modalities that emerged and that may be beneficial for SLE patient care. Keywords: autoantibodies; isotypes; lupus; SLE; Fc receptors; IgE; basophils; plasmacytoid dendritic cells 1. Introduction Systemic lupus erythematosus (SLE) is a chronic systemic autoimmune disease that predominantly affects women of childbearing age. The etiology of SLE is multifactorial and includes environmental, genetic and hormonal factors. SLE evolves by flares that can be triggered by environmental stimuli such as sun exposure or viral infections [1].
    [Show full text]
  • Basophil Activation Receptors Inhibit Mouse and Human Γ Fc
    Fcγ Receptors Inhibit Mouse and Human Basophil Activation Lydie Cassard, Friederike Jönsson, Ségolène Arnaud and Marc Daëron This information is current as of September 24, 2021. J Immunol 2012; 189:2995-3006; Prepublished online 20 August 2012; doi: 10.4049/jimmunol.1200968 http://www.jimmunol.org/content/189/6/2995 Downloaded from Supplementary http://www.jimmunol.org/content/suppl/2012/08/20/jimmunol.120096 Material 8.DC1 References This article cites 61 articles, 24 of which you can access for free at: http://www.jimmunol.org/ http://www.jimmunol.org/content/189/6/2995.full#ref-list-1 Why The JI? Submit online. • Rapid Reviews! 30 days* from submission to initial decision • No Triage! Every submission reviewed by practicing scientists by guest on September 24, 2021 • Fast Publication! 4 weeks from acceptance to publication *average Subscription Information about subscribing to The Journal of Immunology is online at: http://jimmunol.org/subscription Permissions Submit copyright permission requests at: http://www.aai.org/About/Publications/JI/copyright.html Email Alerts Receive free email-alerts when new articles cite this article. Sign up at: http://jimmunol.org/alerts The Journal of Immunology is published twice each month by The American Association of Immunologists, Inc., 1451 Rockville Pike, Suite 650, Rockville, MD 20852 Copyright © 2012 by The American Association of Immunologists, Inc. All rights reserved. Print ISSN: 0022-1767 Online ISSN: 1550-6606. The Journal of Immunology Fcg Receptors Inhibit Mouse and Human Basophil Activation Lydie Cassard,*,†,1 Friederike Jo¨nsson,*,†,1 Se´gole`ne Arnaud,*,† and Marc Dae¨ron*,† Besides high-affinity IgE receptors (Fc«RI), human basophils express activating (FcgRIIA) and inhibitory (FcgRIIB) low-affinity IgG receptors.
    [Show full text]
  • Innovative Approaches to Study Basophil Function in Inflammation
    View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by Publications from Karolinska Institutet From THE DEPARTMENT OF MEDICINE Karolinska Institutet, Stockholm, Sweden INNOVATIVE APPROACHES TO STUDY BASOPHIL FUNCTION IN INFLAMMATION Zenib Aljadi Stockholm 2017 All previously published papers were reproduced with permission from the publisher. Published by Karolinska Institutet. Printed by E-print AB 2017, Stockholm, Sweden © Zenib Aljadi, 2017 ISBN 978-91-7676-792-4 Innovative approaches to study basophil function in inflammation THESIS FOR DOCTORAL DEGREE (Ph.D.) The public defence at Karolinska Institutet will be held at Widerströmska huset, Tomtebodavägen 18A, plan 02, Karolinska Institutet, Solna th Friday, November 10 2017, 13:00 By Zenib Aljadi.MD Principal Supervisor: Opponent: Professor Joachim Lundahl Associated professor Guro Gafvelin Karolinska Institutet Karolinska Institutet Department of Medicine, Solna Department of Clinical Neuroscience Unit of Immunology and Allergy Therapeutic immune design unit Co-supervisor(s): Examination Board: Associated professor Aman Russom Professor Berndt Stegmeyer Royal Institute of Technology Umeå University School of biotechnology Department of Public Health and clinical Medicine Division of proteomics and nanobiotechnology Associated professor Sjoerd Haasl Professor Ola Winqvist Royal Institute of Technology Karolinska Institutet Department of Technology and Health Department of Medicine, Solna Center for Technology in Medicine and Health Unit of Immunology and Allergy Associated professor Nailin Li Associated professor Anna Nopp Karolinska Institutet Karolinska Institutet Department of Medicine Department of Medicine, Solna Division of Clinical Pharmacology Unit of Immunology and Allergy To my parents There are things known and there are things unknown, and in between are the doors for perception.
    [Show full text]