Metabolism and Energy
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Amphibolic Nature of Krebs Cycle
Amphibolic nature of Krebs Cycle How what we are is what we eat • In aerobic organisms, the citric acid cycle is an amphibolic pathway, one that serves in both catabolic and anabolic processes. • Since the citric acid does both synthesis (anabolic) and breakdown (catabolic) activities, it is called an amphibolic pathway • The citric acid cycle is amphibolic (i.e it is both anabolic and catabolic in its function). • It is said to be an AMPHIBOLIC pathway, because it functions in both degradative or catabolic and biosynthetic or anabolic reactions (amphi = both) A central metabolic pathway or amphibolic pathway is a set of reactions which permit the interconversion of several metabolites, and represents the end of the catabolism and the beginning of anabolism • The KREBS CYCLE or citric acid cycle is a series of reactions that degrades acetyl CoA to yield carbon dioxide, and energy, which is used to produce NADH, H+ and FADH. • The KREBS CYCLE connects the catabolic pathways that begin with the digestion and degradation of foods in stages 1 and 2 with the oxidation of substrates in stage 3 that generates most of the energy for ATP synthesis. • The citric acid cycle is the final common pathway in the oxidation of fuel molecules. In stage 3 of metabolism, citric acid is a final common catabolic intermediate in the form of acetylCoA. • This is why the citric acid cycle is called a central metabolic pathway. Anaplerosis and Cataplerosis Anaplerosis is a series of enzymatic reactions in which metabolic intermediates enter the citric acid cycle from the cytosol. Cataplerosis is the opposite, a process where intermediates leave the citric acid cycle and enter the cytosol. -
Lesson 4 Temperature Change TEACHER GUIDE
Lesson 4 Temperature change TEACHER GUIDE Lesson summary Students meet scientist Jason Williams, an industrial chemist who designs the materials and processes for making solar cells. He explains that during the summers, Antarctic days are very long, sometimes lasting a couple of weeks or more. That makes solar energy an abundant natural resource near the South Pole. Solar cells convert energy from the sun into electrical energy. Converting energy from one form to another is an important process. In the activity, students will conduct two chemical reactions that convert chemical energy into thermal energy. Key concepts + A change in temperature is a clue that a chemical reaction may have occurred. + When the temperature increases during a chemical reaction, it is called an exothermic reaction. + When the temperature decreases during a chemical reaction, it is called an endothermic reaction. + It takes energy to break chemical bonds in the reactants. + Energy is released when chemical bonds form in the products. Safety Be sure you and the students wear properly fitting goggles. Read and follow all safety warnings on the labels of the sodium bicarbonate, citric acid, calcium chloride, and universal indicator containers. Also follow the warnings on the packaging of the foot warmer. Have students wash their hands after the activity. Proper disposal At the end of the lesson, have students pour their used solutions in a waste container. The resulting solution from the final demo should be placed in this container, too. Then dispose of all of this liquid waste down the drain or according to local regulations. The left-over sodium bicarbonate, citric acid, and calcium chloride powders can be disposed of with the classroom trash. -
The Citric Acid Cycle the Catabolism of Acetyl-Coa
Al-Sham Private University Faculty Of Pharmacy The Citric Acid Cycle The Catabolism of Acetyl-CoA Lecturer Prof. Abboud Al-Saleh 1 Prof.Abboud AL-Saleh 10/1/2018 BIOMEDICAL IMPORTANCE • The citric acid cycle (Krebs cycle, tricarboxylic acid cycle) is a series of reactions in mitochondria that oxidize acetyl residues (as acetyl-CoA) and reduce coenzymes that upon reoxidation are linked to the formation of ATP. • TCA is the final common pathway for the aerobic oxidation of carbohydrate, lipid, and protein because glucose, fatty acids, and most amino acids are metabolized to acetyl-CoA or intermediates of the cycle. 2 Prof.Abboud AL-Saleh 10/1/2018 • TCA also has a central role in gluconeogenesis, lipogenesis, and interconversion of amino acids. Many of these processes occur in most tissues, but the liver is the only tissue in which all occur to a significant extent. • The repercussions are therefore profound when, for example, large numbers of hepatic cells are damaged as in acute hepatitis or as in cirrhosis. • Very few, if any, genetic abnormalities of TCA enzymes have been reported; such abnormalities would be incompatible with life or normal development 3 Prof.Abboud AL-Saleh 10/1/2018 Summary • The cycle starts with reaction between the acetyl moiety of acetyl-CoA and the four-carbon dicarboxylic acid oxaloacetate, forming a six-carbon tricarboxylic acid, citrate. • In the subsequent reactions, two molecules of CO2 are released and oxaloacetate is regenerated (Figure). • Only a small quantity of oxaloacetate is needed for the oxidation of a large quantity of acetyl-CoA. • oxaloacetate may be considered to play a catalytic role. -
1 Introduction
1 Field-control, phase-transitions, and life’s emergence 2 3 4 Gargi Mitra-Delmotte 1* and A.N. Mitra 2* 5 6 7 139 Cite de l’Ocean, Montgaillard, St.Denis 97400, REUNION. 8 e.mail : [email protected] 9 10 2Emeritius Professor, Department of Physics, Delhi University, INDIA; 244 Tagore Park, 11 Delhi 110009, INDIA; 12 e.mail : [email protected] 13 14 15 16 17 18 19 *Correspondence: 20 Gargi Mitra-Delmotte 1 21 e.mail : [email protected] 22 23 A.N. Mitra 2 24 e.mail : [email protected] 25 26 27 28 29 30 31 32 33 34 35 Number of words: ~11748 (without Abstract, references, tables, and figure legends) 36 7 figures (plus two figures in supplementary information files). 37 38 39 40 41 42 43 44 45 46 47 Abstract 48 49 Critical-like characteristics in open living systems at each organizational level (from bio- 50 molecules to ecosystems) indicate that non-equilibrium phase-transitions into absorbing 51 states lead to self-organized states comprising autonomous components. Also Langton’s 52 hypothesis of the spontaneous emergence of computation in the vicinity of a critical 53 phase-transition, points to the importance of conservative redistribution rules, threshold, 54 meta-stability, and so on. But extrapolating these features to the origins of life, brings up 55 a paradox: how could simple organics-- lacking the ‘soft matter’ response properties of 56 today’s complex bio-molecules--have dissipated energy from primordial reactions 57 (eventually reducing CO 2) in a controlled manner for their ‘ordering’? Nevertheless, a 58 causal link of life’s macroscopic irreversible dynamics to the microscopic reversible laws 59 of statistical mechanics is indicated via the ‘functional-takeover’ of a soft magnetic 60 scaffold by organics (c.f. -
Catabolism Iii
Nitrogen Catabolism Glycogenolysis Protein Fat catabolism Catabolism Fatty Acid Amino-acid GlycolysisDegradation catabolism Pyruvate Oxidation Krebs' Cycle Phosphorylation Oxidative CATABOLISM III: Digestion and Utilization of Proteins • Protein degradation • Protein turnover – The ubiquitin pathway – Protein turnover is tightly regulated • Elimination of nitrogen – By fish, flesh and fowl – How is the N of amino acids liberated and eliminated? • How are amino acids oxidized for energy 1 Protein Catabolism Sources of AMINO ACIDS: •Dietary amino acids that exceed body’s protein synthesis needs •Excess amino acids from protein turnover (e.g., proteolysis and regeneration of proteins) •Proteins in the body can be broken down (muscle wasting) to supply amino acids for energy when carbohydrates are scarce (starvation, diabetes mellitus). •Carnivores use amino acids for energy more than herbivores, plants, and most microorganisms Protein Catabolism The Digestion Pathway • Pro-enzymes are secreted (zymogens) and the environment activates them by specific proteolysis. • Pepsin hydrolyzes protein into peptides in the stomach. • Trypsin and chymotrypsin hydrolyze proteins and larger peptides into smaller peptides in the small intestine. • Aminopeptidase and carboxypeptidases A and B degrade peptides into amino acids in the small intestine. 2 Protein Catabolism The Lysosomal Pathway • Endocytosis, either receptor-mediated, phagocytosis, or pinocytosis engulfs extra- cellular proteins into vesicles. • These internal vesicles fuse as an early endosome. • This early endosome is acidified by the KFERQ Substrates vATPase (“v” for vesicular). • Components that are recycled, like receptors, HSPA8 are sequestered in smaller vesicles to create Co-chaperones the multivesicular body (MVB), sometimes called a late endosome. • If set for degradation, it will fuse with a KFERQ primary lysosome (red) which contains many cathepsin-type proteases. -
CHEM 109A Organic Chemistry
2/23/18 CHEM 109A Organic Chemistry https://labs.chem.ucsb.edu/zakarian/armen/courses.html Chapter 5 Alkene: Introduction Thermodynamics and Kinetics Midterm 2..... Grades will be posted on Tuesday, Feb. 27th . Tests can be picked up outside room CHEM 2138 starting Tuesday, Feb. 27th 1 2/23/18 A Reaction Coordinate Diagram time A reaction coordinate diagram shows the energy changes that take place in each step of a reaction. Thermodynamics and Kinetics Thermodynamics: • are products more stable than starting materials? • overall, are new bonds in products stronger than the old in starting materials? • deals with equilibria Kinetics: • which reaction is faster? Reaction rates • How high is the energy of the transition state? • deals with mechanism of reactions or is Z more stable than Y? 2 2/23/18 The Equilibrium Constant (thermodynamics) The equilibrium constant gives the relative concentration of reactants and products at equilibrium. Exergonic and Endergonic Reactions ∆G° = free energy of the products - free energy of the reactants the relationship between ∆G° and Keq : 3 2/23/18 Exergonic and Endergonic Reactions ∆G° = free energy of the products - free energy of the reactants the relationship between ∆G° and Keq : ∆G° negative: exergonic reaction, products more stable ∆G° positive: endergonic reaction, products less stable Gibbs Free-Energy Change (∆G°) (thermodynamics) 4 2/23/18 Increasing the Amount of a Product Formed in a Reaction (thermodynamics) Le Chatelier’s Principle: if an equilibrium is disturbed, the system will adjust -
Comparison of the Results from Six Calorimeters in the Determination of the Thermokinetics of a Model Reaction Wassila Benaissa, Douglas Carson
Comparison of the results from six calorimeters in the determination of the thermokinetics of a model reaction Wassila Benaissa, Douglas Carson To cite this version: Wassila Benaissa, Douglas Carson. Comparison of the results from six calorimeters in the determina- tion of the thermokinetics of a model reaction. AIChE Spring Meeting 2011 & 7. Global Congress on Process Safety (GCPS), Mar 2011, Chicago, United States. pp.NC. ineris-00976225 HAL Id: ineris-00976225 https://hal-ineris.archives-ouvertes.fr/ineris-00976225 Submitted on 9 Apr 2014 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. Comparison of the Results from Six Calorimeters in the Determination of the Thermokinetics of a Model Reaction Wassila BENAISSA INERIS Parc Technologique Alata BP 2, F-60550 Verneuil-en-Halatte [email protected] Douglas CARSON INERIS Keywords: calorimeter, thermal runway, kinetics Abstract This paper deals with the comparison of experimental results from several types of commercially available calorimeters: a screening calorimeter (DSC), a Calvet calorimeter (C80), a reaction calorimeter (RC1), and various pseudo-adiabatic calorimeters (VSP 2, ARSST, and Phi-Tec 1). One exothermic reaction was selected as a case study: the esterification of acetic anhydride by methanol, a system which has been well studied in the literature. -
Fatty Acid Biosynthesis
BI/CH 422/622 ANABOLISM OUTLINE: Photosynthesis Carbon Assimilation – Calvin Cycle Carbohydrate Biosynthesis in Animals Gluconeogenesis Glycogen Synthesis Pentose-Phosphate Pathway Regulation of Carbohydrate Metabolism Anaplerotic reactions Biosynthesis of Fatty Acids and Lipids Fatty Acids contrasts Diversification of fatty acids location & transport Eicosanoids Synthesis Prostaglandins and Thromboxane acetyl-CoA carboxylase Triacylglycerides fatty acid synthase ACP priming Membrane lipids 4 steps Glycerophospholipids Control of fatty acid metabolism Sphingolipids Isoprene lipids: Cholesterol ANABOLISM II: Biosynthesis of Fatty Acids & Lipids 1 ANABOLISM II: Biosynthesis of Fatty Acids & Lipids 1. Biosynthesis of fatty acids 2. Regulation of fatty acid degradation and synthesis 3. Assembly of fatty acids into triacylglycerol and phospholipids 4. Metabolism of isoprenes a. Ketone bodies and Isoprene biosynthesis b. Isoprene polymerization i. Cholesterol ii. Steroids & other molecules iii. Regulation iv. Role of cholesterol in human disease ANABOLISM II: Biosynthesis of Fatty Acids & Lipids Lipid Fat Biosynthesis Catabolism Fatty Acid Fatty Acid Degradation Synthesis Ketone body Isoprene Utilization Biosynthesis 2 Catabolism Fatty Acid Biosynthesis Anabolism • Contrast with Sugars – Lipids have have hydro-carbons not carbo-hydrates – more reduced=more energy – Long-term storage vs short-term storage – Lipids are essential for structure in ALL organisms: membrane phospholipids • Catabolism of fatty acids –produces acetyl-CoA –produces reducing -
Citric Acid Cycle
CHEM464 / Medh, J.D. The Citric Acid Cycle Citric Acid Cycle: Central Role in Catabolism • Stage II of catabolism involves the conversion of carbohydrates, fats and aminoacids into acetylCoA • In aerobic organisms, citric acid cycle makes up the final stage of catabolism when acetyl CoA is completely oxidized to CO2. • Also called Krebs cycle or tricarboxylic acid (TCA) cycle. • It is a central integrative pathway that harvests chemical energy from biological fuel in the form of electrons in NADH and FADH2 (oxidation is loss of electrons). • NADH and FADH2 transfer electrons via the electron transport chain to final electron acceptor, O2, to form H2O. Entry of Pyruvate into the TCA cycle • Pyruvate is formed in the cytosol as a product of glycolysis • For entry into the TCA cycle, it has to be converted to Acetyl CoA. • Oxidation of pyruvate to acetyl CoA is catalyzed by the pyruvate dehydrogenase complex in the mitochondria • Mitochondria consist of inner and outer membranes and the matrix • Enzymes of the PDH complex and the TCA cycle (except succinate dehydrogenase) are in the matrix • Pyruvate translocase is an antiporter present in the inner mitochondrial membrane that allows entry of a molecule of pyruvate in exchange for a hydroxide ion. 1 CHEM464 / Medh, J.D. The Citric Acid Cycle The Pyruvate Dehydrogenase (PDH) complex • The PDH complex consists of 3 enzymes. They are: pyruvate dehydrogenase (E1), Dihydrolipoyl transacetylase (E2) and dihydrolipoyl dehydrogenase (E3). • It has 5 cofactors: CoASH, NAD+, lipoamide, TPP and FAD. CoASH and NAD+ participate stoichiometrically in the reaction, the other 3 cofactors have catalytic functions. -
The Use of Accelerating Rate Calorimetry (ARC) for the Study of the Thermal Reactions of Li-Ion Battery Electrolyte Solutions J.S
Journal of Power Sources 119–121 (2003) 794–798 The use of accelerating rate calorimetry (ARC) for the study of the thermal reactions of Li-ion battery electrolyte solutions J.S. Gnanaraja, E. Zinigrada, L. Asrafa, H.E. Gottlieba, M. Sprechera, D. Aurbacha,*, M. Schmidtb aDepartment of Chemistry, Bar-Ilan University, Ramat-Gan 52900, Israel bMerck KGaA, D-64293 Darmstadt, Germany Abstract The thermal stability of 1M LiPF6, LiClO4, LiN(SO2CF2CF3)2 (LiBETI) and LiPF3(CF2CF3)3 (LiFAP) solutions in mixtures of ethylene carbonate, diethyl carbonate and dimethyl carbonate in the temperature range 40–350 8C was studied by ARC and DSC. NMR was used to analyze the reaction products at different reaction stages. The least thermally stable are LiClO4 solutions. LiPF3(CF2CF3)3 solutions showed higher thermal stability than LiPF6 solutions. The highest thermal stability was found for LiN(SO2CF2CF3)2 solutions. Studies by DSC and pressure measurements during ARC experiments with LiPF6 and LiFAP solutions detected an endothermic reaction, which occurs before a number of exothermic reactions as the temperature increases. Fluoride ions are formed and react with the alkyl carbonate molecules both as bases and as nucleophiles. # 2003 Elsevier Science B.V. All rights reserved. Keywords: Accelerating rate calorimetry (ARC); Differential scanning calorimetry (DSC); Thermal stability; Alkyl carbonate solutions 1. Introduction 2. Experimental Accelerating rate calorimetry (ARC) is an important One molar LiPF6, LiClO4, LiN(SO2CF2CF3)2 and method for studying the thermal behavior of materials LiPF3(CF2CF3)3 solutions in mixture of ethylene carbonate [1,2]. LiPF6 solutions in alkyl carbonates are widely used (EC), diethyl carbonate (DEC), dimethyl carbonate (DMC) in commercial Li-ion batteries in spite of their relatively low (2:1:2 v/v/v) were obtained from Merck KGaA (highly pure, thermal stability. -
ATP and Cellular Work | Principles of Biology from Nature Education
contents Principles of Biology 23 ATP and Cellular Work ATP provides the energy that powers cells. Magnetic resonance images of three different areas in the rat brain show blood flow and the biochemical measurements of ATP, pH, and glucose, which are all measures of energy use and production in brain tissue. The image is color-coded to show spatial differences in the concentration of these energy-related variables in brain tissue. © 1997 Nature Publishing Group Hoehn-Berlage, M., et al. Inhibition of nonselective cation channels reduces focal ischemic injury of rat brain. Journal of Cerebral Blood Flow and Metabolism 17, 534–542 (1997) doi: 10.1097/00004647-199705000-00007. Used with permission. Topics Covered in this Module Using Energy Resources For Work ATP-Driven Work Major Objectives of this Module Describe the role of ATP in energy-coupling reactions. Explain how ATP hydrolysis performs cellular work. Recognize chemical reactions that require ATP hydrolysis. page 116 of 989 4 pages left in this module contents Principles of Biology 23 ATP and Cellular Work Energy is a fundamental necessity for all of life's processes. Without energy, flagella cannot move, DNA cannot be unwound or separated for replication or gene expression, cells cannot divide, plants cannot grow and animals cannot reproduce. Energy is vital, but where does it come from? Plants and photosynthetic microbes capture light energy and convert it into chemical energy for their own use. Organisms that cannot produce their own food, such as fungi and animals, feed upon this captured energy. However, the chemical energy produced by photosynthesizers needs to be converted into a usable form. -
What Makes Things Go Boom?
Curriculum Units by Fellows of the Yale-New Haven Teachers Institute 2016 Volume IV: Physical Science and Physical Chemistry What Makes Things Go Boom? Curriculum Unit 16.04.05 by Ariella Iancu Introduction Most students point to Walter White as a chemistry anti-hero—using crystalized fulminated mercury, disguised as crystal meth, as a grenade to blow up a drug lord that wronged him (Hughes, 2008). Yet, over the year, students often feel bogged down by seemingly random analytical calculations and mountains of memorization. While students typically do bring a passion for labs, they have a harder time making connections between what they see, and the quantitative analysis necessary to understand why a particular phenomenon occurred. It is hard for them to see that for Walter White to cause the mercury fulminate explosion, he needed to understand the stability of the compound (entropy), at what speed he would need to throw it to provide enough energy to activate the change (activation energy), and how much heat is released (enthalpy) so that he himself does not die in the building. While the mercury fulminate bomb represents a chemical change, the principle of energy, entropy, and enthalpy can also be applied to dangerous physical changes—such as an over-heating water heater (Dallow & Lentle, 2007). Heat up the water too fast or too high, and the water heater could explode. By understanding principles of thermodynamics, including the incorporation of mathematical analyses, students can apply the underlying reasons for chemical and physical change to create products and solve problems. The focus of this unit to keep the chemistry relevant and exciting to students while keeping the mathematical skills present and strong.