SANTA CRUZ BIOTECHNOLOGY, INC. Hbb-bh1 shRNA (m) Lentiviral Particles: sc-145900-V

BACKGROUND APPLICATIONS , , neuroglobin and are porphyrin-containing Hbb-bh1 shRNA (m) Lentiviral Particles is recommended for the inhibition of that belong to the family and function in oxygen transport Hbb-bh1 expression in mouse cells. and storage. Hemoglobin contributes to oxygen storage and diffusion of oxy- gen in blood tissue. Hbb-bh1 (hemoglobin subunit β-H1), also known as SUPPORT REAGENTS β Z or H1, is a 147 amino acid protein that belongs to the globin Control shRNA Lentiviral Particles: sc-108080. Available as 200 µl frozen α β family. Existing as a heterotetramer of two and two chains, Hbb-bh1 is viral stock containing 1.0 x 106 infectious units of virus (IFU); contains an expressed in red blood cells. The gene encoding Hbb-bh1 maps to mouse shRNA construct encoding a scrambled sequence that will not lead to the chromosome 7 E3. The human homolog to Hbb-bh1, known as Hemoglobin specific degradation of any known cellular mRNA. or HBG1, is expressed in fetal liver, spleen and bone marrow until four or γ five weeks after birth. (HbF), which consists of two and RT-PCR REAGENTS two α chains, is replaced by adult hemoglobin (HbA) at birth. Semi-quantitative RT-PCR may be performed to monitor Hbb-bh1 gene REFERENCES expression knockdown using RT-PCR Primer: Hbb-bh1 (m)-PR: sc-145900-PR (20 µl). Annealing temperature for the primers should be 55-60° C and the β 1. Jahn, C.L., et al. 1980. DNA sequence organization of the -globin com- extension temperature should be 68-72° C. plex in the BALB/c mouse. Cell 21: 159-168. 2. Slightom, J.L., et al. 1980. Human fetal G γ- and A γ-globin genes: com- BIOSAFETY plete nucleotide sequences suggest that DNA can be exchanged between Lentiviral particles can be employed in standard Biosafety Level 2 tissue these duplicated genes. Cell 21: 627-638. culture facilities (and should be treated with the same level of caution as 3. Shen, S.H., et al. 1981. A history of the human fetal globin gene duplica- with any other potentially infectious reagent). Lentiviral particles are repli- tion. Cell 26: 191-203. cation-incompetent and are designed to self-inactivate after transduction and integration of shRNA constructs into genomic DNA of target cells. 4. Hill, A., et al. 1984. Two mouse early embryonic β-globin gene sequences. β Evolution of the nonadult -. J. Biol. Chem. 259: 3739-3747. RESEARCH USE 5. Shehee, W.R., et al. 1989. Nucleotide sequence of the BALB/c mouse The purchase of this product conveys to the buyer the nontransferable right β-globin complex. J. Mol. Biol. 205: 41-62. to use the purchased amount of the product and all replicates and derivatives 6. Suzuki, N., et al. 2011. Analysis of altered gene expression specific to for research purposes conducted by the buyer in his laboratory only (whether embryotoxic chemical treatment during embryonic stem cell differentiation the buyer is an academic or for-profit entity). The buyer cannot sell or other- into myocardiac and neural cells. J Toxicol Sci. 36: 569-585. wise transfer (a) this product (b) its components or (c) materials made using this product or its components to a third party, or otherwise use this product CHROMOSOMAL LOCATION or its components or materials made using this product or its components for Commercial Purposes. Genetic locus: Hbb-bh1 (mouse) mapping to 7 E3. PROTOCOLS PRODUCT See our web site at www.scbt.com for detailed protocols and support Hbb-bh1 shRNA (m) Lentiviral Particles are concentrated, transduction-ready products. viral particles containing a target-specific construct that encodes a 19-25 nt (plus hairpin) shRNA designed to knock down gene expression. Each vial contains 200 µl frozen stock containing 1.0 x 106 infectious units of virus (IFU) in Dulbecco’s Modified Eagle’s Medium with 25 mM HEPES pH 7.3. Suitable for 10-20 transductions. Also see Hbb-bh1 siRNA (m): sc-145900 and Hbb-bh1 shRNA Plasmid (m): sc-145900-SH as alternate gene silencing products.

STORAGE Store lentiviral particles at -80º C. Stable for at least one year from the date of shipment. Once thawed, particles can be stored at 4º C for up to one week. Avoid repeated freeze thaw cycles.

Santa Cruz Biotechnology, Inc. 1.800.457.3801 831.457.3800 fax 831.457.3801 Europe +00800 4573 8000 49 6221 4503 0 www.scbt.com