SANTA CRUZ BIOTECHNOLOGY, INC. Ameloblastin (H-2): sc-271012

BACKGROUND APPLICATIONS Dental enamel is a highly mineralized tissue with most of its volume occu- Ameloblastin (H-2) is recommended for detection of Ameloblastin of human pied by large, highly organized, hydroxyapatite crystals. This structure is origin by Western Blotting (starting dilution 1:100, dilution range 1:100- thought to be controlled through the interaction of many organic matrix mole- 1:1000), immunoprecipitation [1-2 µg per 100-500 µg of total (1 ml cules including , Ameloblastin, , and several of cell lysate)], immunofluorescence (starting dilution 1:50, dilution range other enzymes. All of these secreted are involved in the mineraliza- 1:50-1:500), immunohistochemistry (including paraffin-embedded sections) tion and enamel matrix formation in developing . Ameloblastin (starting dilution 1:50, dilution range 1:50-1:500) and solid phase ELISA (AMBN), which localizes to the extracellular matrix, is an -specific (starting dilution 1:30, dilution range 1:30-1:3000). protein. It is detected in the sheath space between rod-interrod enamel and Suitable for use as control antibody for Ameloblastin siRNA (h): sc-44945, at the Tomes processes of secretory . Defects in the encod- Ameloblastin shRNA Plasmid (h): sc-44945-SH and Ameloblastin shRNA (h) ing for Ameloblastin, AMBN, can be seen in patients with ameloblastomas. Lentiviral Particles: sc-44945-V. REFERENCES Molecular Weight of Ameloblastin: 48 kDa. 1. MacDougall, M., et al. 2000. Cloning, characterization and immunolocal- ization of human Ameloblastin. Eur. J. Oral Sci. 108: 303-310. RECOMMENDED SUPPORT REAGENTS To ensure optimal results, the following support reagents are recommended: 2. Toyosawa, S., et al. 2000. Cloning and characterization of the human 1) Western Blotting: use m-IgGκ BP-HRP: sc-516102 or m-IgGκ BP-HRP (Cruz Ameloblastin gene. Gene 256: 1-11. Marker): sc-516102-CM (dilution range: 1:1000-1:10000), Cruz Marker™ 3. Mardh, C.K., et al. 2001. Human Ameloblastin gene: genomic organization Molecular Weight Standards: sc-2035, UltraCruz® Blocking Reagent: and mutation analysis in imperfecta patients. Eur. J. Oral Sci. sc-516214 and Western Blotting Luminol Reagent: sc-2048. 2) Immunopre- 109: 8-13. cipitation: use Protein A/G PLUS-Agarose: sc-2003 (0.5 ml agarose/2.0 ml). 3) Immunofluorescence: use m-IgGκ BP-FITC: sc-516140 or m-IgGκ BP-PE: 4. Shintani, S., et al. 2005. Expression of Ameloblastin during enamel forma- sc-516141 (dilution range: 1:50-1:200) with UltraCruz® Mounting Medium: tion in a crocodile. Enamel formation, as well as adhesion between sc-24941 or UltraCruz® Hard-set Mounting Medium: sc-359850. 4) Immuno- ameloblasts and the enamel. J. Exp. Zool. B Mol. Dev. Evol. 306: 126-133. histochemistry: use m-IgGκ BP-HRP: sc-516102 with DAB, 50X: sc-24982 5. Torres-Quintana, M.A., et al. 2005. Ameloblastin and Amelogenin expres- and Immunohistomount: sc-45086, or Organo/Limonene Mount: sc-45087. sion in posnatal developing mouse molars. J. Oral Sci. 47: 27-34. 6. Wang, H., et al. 2005. Enamel matrix protein interactions. J. Bone Miner. DATA Res. 20: 1032-1040.

CHROMOSOMAL LOCATION 132 K – 90 K – Ameloblastin Genetic locus: AMBN (human) mapping to 4q13.3. fusionprotein 55 K –

SOURCE 43 K – Ameloblastin (H-2) is a mouse monoclonal antibody raised against amino acids 148-447 mapping at the C-terminus of Ameloblastin of human origin. Ameloblastin (H-2): sc-271012.Western blot analysis Ameloblastin (H-2): sc-271012. Immunoperoxidase of human recombinant Ameloblastin fusionprotein. staining of formalin fixed, paraffin-embedded human PRODUCT kidney tissue showing cytoplasmic staining of cells in glomeruli and cells in tubules. Each vial contains 200 µg IgG1 kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin. SELECT PRODUCT CITATIONS Ameloblastin (H-2) is available conjugated to agarose (sc-271012 AC), 1. Stakkestad, O., et al. 2018. An Ameloblastin C-terminus variant is present 500 µg/0.25 ml agarose in 1 ml, for IP; to HRP (sc-271012 HRP), 200 µg/ml, in human adipose tissue. Heliyon 4: e01075. for WB, IHC(P) and ELISA; to either phycoerythrin (sc-271012 PE), fluorescein (sc-271012 FITC), Alexa Fluor® 488 (sc-271012 AF488), Alexa Fluor® 546 STORAGE (sc-271012 AF546), Alexa Fluor® 594 (sc-271012 AF594) or Alexa Fluor® 647 (sc-271012 AF647), 200 µg/ml, for WB (RGB), IF, IHC(P) and FCM; and to either Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of Alexa Fluor® 680 (sc-271012 AF680) or Alexa Fluor® 790 (sc-271012 AF790), shipment. Non-hazardous. No MSDS required. 200 µg/ml, for Near-Infrared (NIR) WB, IF and FCM. Alexa Fluor® is a trademark of Molecular Probes, Inc., Oregon, USA

RESEARCH USE For research use only, not for use in diagnostic procedures.

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