Recombinant Human Cystatin S/CST4/Cystatin-4 Protein

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Catalog No.: RP00340 Recombinant

Sequence Information Background

Species Gene ID Swiss Prot The cystatin superfamily encompasses proteins that contain multiple cystatin-like Human 1472 P01036 sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three Tags inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 6×His tag at the C-terminus cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions. The Synonyms cystatin locus on chromosome 20 contains the majority of the type 2 cystatin genes #N/A and pseudogenes. This gene is located in the cystatin locus and encodes a type 2 salivary cysteine peptidase inhibitor. The protein is an S-type cystatin, based on its high level of expression in saliva, tears and seminal plasma. The specific role in these fluids is unclear but antibacterial and antiviral activity is present, consistent with a protective function. Product Information

Source Purification Basic Information Human cells > 95% by SDS- PAGE. Description Recombinant Human Cystatin S/CST4/Cystatin-4 Protein is produced by Human Endotoxin Cell expression system. The target protein is expressed with sequence (Ser21- < 1 EU/μg of the protein by LAL Ala141) of human Cystatin S/CST4/Cystatin-4 (Accession #P01036) fused with a method. 6×His tag at the C-terminus.

Formulation Bio-Activity Lyophilized from a 0.2 μm filtered Measured by its ability to inhibit active Cathepsin L cleavage of a fluorogenic solution of 20 mM MES, 150 mM NaCl, peptide substrate Z-LR-AMC. The IC50 value is<8.0 nM. pH 5.5. Contact us for customized product Storage form or formulation. Store the lyophilized protein at -20°C to -80 °C for long term. After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C Reconstitution for up to 1 week. Reconstitute to a concentration of Avoid repeated freeze/thaw cycles. 0.1-0.5 mg/mL in sterile distilled water.

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Recombinant protein Human Cystatin S/CST4/Cystatin-4 was determined by SDS- PAGE under reducing conditions with Coomassie Blue, showing a band at 15 kDa.