BORIS (CTCFL) Mouse Monoclonal Antibody [Clone ID: 4A7] – TA353114L | Origene

OriGene Technologies, Inc. 9620 Medical Center Drive, Ste 200 Rockville, MD 20850, US Phone: +1-888-267-4436 [email protected] EU: [email protected] CN: [email protected] Product datasheet for TA353114L

BORIS (CTCFL) Mouse Monoclonal Antibody [Clone ID: 4A7] Product data:

Product Type: Primary Antibodies Clone Name: 4A7 Applications: WB Recommended Dilution: ChIP ; ELISA ; IF ; IP ; WB Reactivity: Human Host: Mouse Isotype: IgM, kappa Clonality: Monoclonal Immunogen: The N-terminal domain (aa 2-257) of human BORIS (CTCFL) protein, expressed in E.coli Formulation: PBS with 0.02% Sodium Azide Concentration: lot specific Purification: Purified by affinity chromatography. Conjugation: Unconjugated Storage: Store at -20°C as received. Stability: Stable for 12 months from date of receipt. Predicted Protein Size: 83 Gene Name: CCCTC-binding factor like Database Link: NP_542185 Entrez Gene 140690 Human Q8NI51 Background: Reagent for research, diagnostic tool. BORIS protein has been identified as Cancer-Testis Antigen (CTA) with testis-specific paralogue of the CCCTC-binding factor. Recent studies have demonstrated that d BORIS is directly responsible for the transcriptional activation of TSP50 (testes-specific protease 50). Synonyms: BORIS; CT27; CTCF-T; dJ579F20.2; HMGB1L1 Note: Protein L beads should be used for ChIP and IP because this antibody is IgM kappa Protein Families: Transcription Factors

This product is to be used for laboratory only. Not for diagnostic or therapeutic use. View online » ©2021 OriGene Technologies, Inc., 9620 Medical Center Drive, Ste 200, Rockville, MD 20850, US 1 / 2 BORIS (CTCFL) Mouse Monoclonal Antibody [Clone ID: 4A7] – TA353114L

Product images:

Agarose gel analyses of PCR products from ChIP assays using mouse anti-BORIS IgM antibody immobilized on Protein L agarose beads. A. PCR products were tested using primers for the known CTS within the human PIM-1 gene. Both BORIS and CTCF shows DNA enrichment at the PIM-1 site. B. PCR perfomed for another characterized CTS (H19ICR). A stronger binding at the H19 site in CTCF ChIPDNA was observed as compared to BORIS ChIP DNA. No antibody reactions for both targets do not show any PCR product. In all ChIP experiments, 1% of sonicated cross-linked cell lysate was used as positive control (Input).