R&D Systems Tools for Cell Biology Research™

New Products MARCH 2013

New GDF-8/Myostatin Quantikine® ELISA Kit Contents Growth Differentiation Factor 8 (GDF-8), also known as Myostatin, is a secreted TGF-β super­ Recombinant GMP Proteins 2 50000 family protein that is expressed in developing and adult skeletal muscle. GDF-8 controls myoblast 45000 Recombinant Proteins 3 proliferation and is a potent negative regulator of 40000 skeletal muscle mass.1 Synthesized as a 376 amino 35000 acid (aa) preproprotein, GDF-8 consists of a 24 aa 30000 Polyclonal Antibodies 3-4 signal peptide, a 243 aa propeptide, and a 109 aa 25000 2 polypeptide that forms the mature protein. The 20000 Monoclonal Antibodies 4-6 secreted proprotein is cleaved by BMP-1 family 15000 Serum GDF-8 (pg/mL) proteases to separate the 35-40 kDa propeptide 10000 from the 12 kDa bioactive mature protein.3 This 5000 Fluorochrome-labeled Antibodies 6-8 cleavage results in a latent complex containing a 0 disulfide-linked dimer of the mature protein and Human Mouse Rat Porcine Canine Equine (n=35) (n=10) (n=10) (n=10) (n=10) (n=4) GloLive™ Monoclonal Antibodies 8 two noncovalently-associated propeptides.2,4 Within the propeptide, mouse GDF-8 shares 96% Detection of GDF-8/Myostatin in Serum. Levels of GDF-8/Myostatin were Biotinylated Antibodies 8 and 99% aa sequence identity with human and rat detected in serum from multiple species using the GDF-8/Myostatin Quantikine GDF-8, respectively. The amino acid sequence of ELISA Kit (Catalog # DGDF80). Samples were pre-treated and diluted to within mature GDF-8 is 100% conserved between human, the range of the standard curve, following the protocol provided in the product Secondary Antibodies 8 mouse, and rat. datasheet. The average concentration and standard deviation for each group of DuoSet® ELISA and DuoSet IC ELISA samples are presented after correction for the respective dilution factor. Development Systems 9 Active GDF-8 binds to the type II Activin receptor N values represent the number of animals tested in each group. Activin RIIB which then associates with the type I ELISpot Kits and Development receptors Activin RIB/ALK-4 or TGF-β RI/ALK-5 to Modules 9 induce signaling.5 GDF-8 is upregulated in cardio- References myocytes surrounding infarcted areas after mycar- 1. Lee, S.J. et al. (2010) Immunol. Endocr. Metab. Agents 6 Proteome Profiler™ Antibody Arrays 10 dial infarction. It is released by mechanically- Med. Chem. 10:183. stressed cardiomyocytes and induces the skeletal 2. McPherron, A.C. et al. (1997) Nature 387:83. 7,8 muscle wasting, which is common in heart failure. 3. Wolfman, N.M. et al. (2003) Proc. Natl. Acad. Sci. USA Luminex® Performance Assays 10 Circulating levels of GDF-8 are elevated following 100:15842. pressure overload-induced cardiac hypertrophy.7 4. Lee, S.J. et al. (2001) Proc. Natl. Acad. Sci. USA 98:9306. Fluorokine® Flow Cytometry Kits 10 R&D Systems now offers a GDF-8/Myostatin 5. Rebbapragada, A. et al. (2003) Mol. Cell. Biol. 23:7230. Quantikine ELISA Kit (Catalog # DGDF80) that is 6. Sharma, M. et al. (1999) J. Cell. Physiol. 180:1. Cell-Based ELISA Assay Kits 10 designed to measure human, mouse, rat, canine, 7. Heineke, J. et al. (2010) Circulation 121:419. porcine, and equine GDF-8/Myostatin in cell culture 8. Breitbart, A. et al. (2011) Am. J. Physiol. Heart Circ. supernates, tissue homogenates, serum, EDTA Physiol. 300:H1973. Stem Cell Products 10 plasma, and Heparin plasma. For additional information, and for products related to the TGF-β VersaClone™ cDNA Plasmids 11-12 Superfamily, please visit our website at www.RnDSystems.com/TGFbeta. Tocris Biochemicals & Compounds 13-15 Boston Biochem® Ubiquitin-related Quantikine® ELISA Kits Research Products 16 Analyte Species Sensitivity Range Catalog # Size Price Cyr61/CCN1 Human 3.8 pg/mL 39.1-2,500 pg/mL DCYR10 1 Kit $515 GDF-8/Myostatin Multi-species 5.32 pg/mL 31.3-2,000 pg/mL DGDF80 1 Kit $515 PDGF-CC Human 24.7 pg/mL 62.5-4,000 pg/mL DCC00 1 Kit $515

Additional ELISA Kits Analyte Species Sensitivity Range Catalog # Size Price Free IGF-I Human 0.015 ng/mL 0.094-6 ng/mL DFG100 1 Kit $515

www.RnDSystems.com Integrin α6β1 New GMP-grade Recombinant Proteins Integrins are heterodimeric cell-surface receptors R&D Systems now offers GMP-grade cytokines and growth factors for research use and further composed of non-covalently associated α and β manufacturing applications where current Good Manufacturing Practices (cGMPs) are required. GMP subunits that are classified into subfamilies based proteins are manufactured in our ISO-certified facility in compliance with relevant guidelines.1 New on ligand-binding properties or subunit compo- GMP-grade proteins include Recombinant Human GM-CSF, Recombinant Human IL-4, and Recombinant sition.1 Integrin α6β1, also called Platelet Human/Mouse/Rat Activin A. Additional GMP-grade cytokines will be available in the next several Glycoprotein GPIc-IIa, is a heterodimer of Integrin months. For an up-to-date product listing or additional information on GMP proteins, please visit our α6 (CD49f) and Integrin β1 (CD29). Integrin α6β1 website at www.RnDSystems.com/GMP. is one of four integrins that function as a laminin receptor.1 It is expressed by many cell types 10000 including T cells, endothelial cells, stem cells, and GMP Protein Source Catalog # Size Price 2-6 platelets. It appears to be essential for the Human/Mouse/Rat CHO 338-GMP-010 10 µg $295 9000 attachment, expansion, and self-renewal of human Activin A 8000 and mouse pluripotent stem cells.4,5 It also has 338-GMP-050 50 µg $835 been shown to promote lymphangiogenesis.7 338-GMP-01M 1 mg $7,600 7000 Additionally, Integrin α6β1 expression is upregu- Human GM-CSF E. coli 215-GMP-010 10 µg $285 6000 lated in many cancers, including prostate, glioma, and hepatoma, where it is believed to enhance 215-GMP-050 50 µg $1,035 5000 tumorigenicity, motility, invasion, and metastasis.8-10 (Mean RFU) Proliferation Cell 215-GMP-01M 1 mg $4,200 4000 R&D Systems now offers several new recombinant Human IL-4 E. coli 204-GMP-010 10 µg $265 3000 human integrins including Recombinant Human 204-GMP-050 50 µg $885 10-4 10-3 10-2 10-1 100 (Catalog # 7809-A6) and Mouse (Catalog # 7810-A6) Recombinant Human GM-CSF GMP (ng/mL) Integrin α6β1. Please see page 3 for details or visit our 204-GMP-01M 1 mg $5,500 website at www.RnDSystems.com/Integrins for Bioactivity of GMP-grade Recombinant Human GM-CSF. GMP-grade additional Integrin-related products. Recombinant Human GM-CSF (Catalog # 215-GMP) stimulates prolifera-

tion of the TF-1 human erythroleukemic cell line. The ED50 is typically 6-30 pg/mL.

3.0 kDa 190 2.5 7000 14468 6000 2.0 92.5 5000 7234 1.5 66 4000 55 1.0 43 Peak Intensity Peak 3000 Laminin α 4 Binding 36 0.1 29 2000

0 21 1000 7347 14673 18.4 0.0001 0.001 0.01 0.1 1 10 12.4 0 Integrin α6β1 (µg/mL) 6000 9000 12000 15000 18000 21000 24000 6.3 Mass Charge Ratio Recombinant Integrin α6β1 Binds Laminin α4. Recombinant Human Laminin α4 (Catalog # 7340-A4) was coated onto a microplate well at 5 μg/mL. Purity of GMP-grade Recombinant Human GM-CSF. 1 μg of GMP-grade MALDI-TOF Analysis of GMP-grade Recombinant Human GM-CSF. Recombinant Human (Catalog # 7809-A6; green) Integrin α6β1 binds Laminin Recombinant Human GM-CSF (Catalog # 215-GMP) was resolved by SDS-PAGE under GMP-grade Recombinant Human GM-CSF (Catalog # 215-GMP) was α4 in a dose-dependent manner. reducing conditions and visualized by silver staining. analyzed by MALDI-TOF. The major peak corresponds to the calculated molecular mass of 14468 Da. The minor peak at 14673 Da is a matrix- associated artifact of the MALDI-TOF. References 1. Barczyk, M. et al. (2010) Cell Tissue Res. 339:269. Features of GMP-grade Proteins: 2. Mojcik, C.F. et al. (1995) Blood 86:4206. 3. Albelda, S.M. & C.A. Buck (1990) FASEB J. 4:2868.  Extensive documentation at every stage of  Proven formulations to ensure consistent 4. Rodin, S. et al. (2010) Nat. Biotech. 28:611. development from cell culture to final fill reconstitution and results and formulation 5. Domogatskaya, A. et al. (2008) Stem Cells 26:2800.  Validated equipment and trained employees 6. Tamura, R.N. et al. (1990) J. Cell Biol. 111:1593.  Documentation of lot-to-lot consistency and  Lot-specific Certificate of Analysis with each traceability of materials used 7. Larrieu-Lahargue, F. et al. (2011) Circ. Res. 109:770. shipment 8. Sroka, I.C. et al. (2011) Mol. Cancer Res. 9:1319.  Rigorous quality control program using 9. Dai, J.Y. et al. (2009) BMC Cancer 9:337. stringent analytical processes 10. Delamarre, E. et al. (2009) Am. J. Pathol. 175:844.

1Manufactured in compliance of the applicable sections of World Health Organization TRS, No 822, 1992 Annex 1, Good Manufacturing Practices for biological products; USP Chapter 1043, Ancillary Materials for Cell, Gene, and Tissue-Engineered Products, and USP Chapter 92, Cytokines used in Cell Therapy Manufacturing.

2 For research use only. Not for use in diagnostic procedures. Recombinant Proteins Interleukin-24 Interleukin-24, also known as Melanoma Protein Species Source Catalog # Size Price Differentiation Antigen 7 (MDA-7) and IL-4-Induced ADAM23 Human CHO 4974-AD-050 50 µg $319 Secreted Protein (FISP), is a member of the Biotinidase/BTD Human CHO 7839-BT-010 10 µg $319 IL-10 family of cytokines. It is secreted by activated monocytes, macrophages, Th2 cells, B cells, natural C1q R1/CD93 Fc Chimera Human NS0 2379-CD-050 50 µg $319 killer cells, keratinocytes, and melanocytes.1,2 DCBLD2/ESDN Human NS0 6269-DC-050 50 µg $319 IL-24 signals through two heterodimeric receptor complexes, one composed of IL-20 Ra and Follistatin-like 1/FSTL1 Mouse NS0 1738-FN-050 50 µg $319 IL-20 Rb and the other composed of IL-22 Ra1 and Frizzled-9 Fc Chimera Mouse CHO 2440-FZ-050 50 µg $319 IL-20 Rb.3 Both IL-24 receptor complexes are Glycerol Kinase E. coli E. coli 7849-GK-020 20 µg $319 primarily expressed in the skin, lung, and reproductive tissues.4 Although IL-24 receptors have GPR111 Human CHO 6494-GP-050 50 µg $319 not been found on hematopoietic cells, IL-24 has HIN-1/SCGB3A1 Human E. coli 2790-HN-050 50 µg $319 been shown to inhibit plasma cell differentiation in germinal center B cells and to induce secretion of IFN-α/β R1 Human NS0 245-AB-050 50 µg $319 high levels of IL-6, TNF-a, and IFN-g from human IL-10 Rα* Mouse Sf 21 7704-MR-025 25 µg $405 blood leukocytes.5,6 Based on this activity and its IL-15 Feline E. coli 7805-FL-010 10 µg $289 expression pattern, IL-24 has been suggested to be involved in wound healing and inflammation in the IL-24* Mouse NS0 7807-ML-010 10 µg $289 skin.1,3 In addition to these effects, adenoviral- Integrin α11β1 Mouse CHO 7808-AB-050 50 µg $319 mediated ectopic expression of IL-24 has been shown to selectively promote apoptosis in a variety Integrin α2β1 Mouse CHO 7828-A2-050 50 µg $319 of different tumor cells, while not affecting the Integrin α6β1 Human CHO 7809-A6-050 50 µg $319 growth or viability of normal cells.7,8 Since this Integrin α6β1 Mouse CHO 7810-A6-050 50 µg $319 response is not mediated through activation of the IL-24 receptor, it is currently unclear whether secreted Integrin α10β1 Mouse CHO 7827-AB-050 50 µg $319 IL-24 contributes to this effect.6 LAG-3 Fc Chimera Mouse NS0 3328-L3-050 50 µg $319 R&D Systems now offers NS0-derived Recombinant Leptin R Fc Chimera* Mouse NS0 7814-LR-050 50 µg $289 Mouse IL-24 (Catalog # 7807-ML), which is 1000-fold more active than E. coli-derived Recombinant Mouse MMP-17 Human NS0 7796-MP-010 10 µg $315 IL-24. For more information, please visit our website at NAALADase-2/NAALAD2 Human CHO 7658-ZN-010 10 µg $319 www.RnDSystems.com/IL10-InterferonFamily NELL1 Mouse CHO 7109-NL-050 50 µg $319 Neuronal Pentraxin 2 Human NS0 7816-NP-050 50 µg $319 5200 β-NGF* Rat CHO 7815-NG-025 25 µg $229

PILR-α Human NS0 6484-PR-050 50 µg $319 4200 PlGF-3 Human Sf 21 7758-PL-025 25 µg $319

PNGase F' (Cystatin A Tag)* F. meningosepticum E. coli 7695-GHP-020 20 µg $200 3200 Ryk Fc Chimera Mouse NS0 4649-RK-050 50 µg $319 SECTM1A Fc Chimera Mouse NS0 7837-ST-050 50 µg $319 2200 Cell Proliferation (Mean RFU) Proliferation Cell Sirtuin 1/SIRT1 Human E. coli 7714-DA-050 50 µg $319 * New and improved version 1200 0.0001 0.001 0.01 0.1 1 10 100 IL-24 (ng/mL) Polyclonal Antibodies IL-24 Promotes Cell Proliferation. BaF3 mouse pro-B cells transfected with Antibody Species Application Catalog # Size Price human IL-20 Ra and human IL-20 Rb were grown in the presence of increasing concentrations of Recombinant Mouse IL-24 (Catalog # 7807-ML). Cell Afadin/AF-6 Human IHC, WB AF7829 100 µg $359 proliferation was assessed in a fluorometric assay using the redox sensitive ALCAM/CD166 Mouse/Rat FC, ICC, IHC, WB AF1172 100 µg $359 dye Resazurin (Catalog # AR002).

Aldehyde Dehydrogenase 3-A1/ALDH3A1 Human WB AF6705 100 µg $359 References ALK/CD246 Human IHC, WB AF4210 100 µg $359 1. Sahoo, A. & S-H. Im (2012) Immune Netw. 12:1. 2. Wang, M. & P. Liang (2005) Immunology 114:166. Brachyury Human/Mouse ChIP, ICC, IHC, WB AF2085 100 µg $359 3. Wang, M. et al. (2002) J. Biol. Chem. 277:7341. Carboxypeptidase M Human WB AF7457 100 µg $359 4. Sabat, R. (2010) Cytokine Growth Factor Rev. 21:315. CART Human/Mouse/Rat IHC, WB AF163 100 µg $359 5. Caudell, E.G. (2002) J. Immunol. 168:6041. Chk1 Human/Mouse/Rat IP, WB AF1630 100 µg $359 6. Maroof, G. et al. (2010) Blood 115:1718. 7. Fisher, P.B. (2005) Cancer Res. 65:10128. Cysteine Conjugate β-Lyase/CCBL1 Human/Mouse/Rat WB AF7817 100 µg $359 8. Emdad, L. (2009) Cancer Biol. Ther. 8:391. DAPP1 Human/Mouse WB AF7024 100 µg $359 Continued on page 4.

www.RnDSystems.com 3 Phospho-Akt (S473) Polyclonal Antibodies The Akt family of serine/threonine protein kinases, Antibody Species Application Catalog # Size Price also known as protein kinase B (PKB), is comprised of three highly homologous members: Akt1 (PKBα), Continued from page 3. Akt2 (PKBβ), and Akt3 (PKBγ). These kinases are Glucosaminyl (N-acetyl) Transferase 1/GCNT1 Human ICC, WB AF7248 100 µg $359 central to many cellular processes including glucose uptake, cell proliferation, and apoptosis. In addition, GFAP Human/Rat ICC, WB AF2594 100 µg $359 their activity is often dysregulated in many cancer KLF5 Human/Mouse ICC, WB AF3758 100 µg $359 types.1 Full activation of Akt by growth factors is Klotho β Human ICC, WB AF5889 100 µg $359 dependent on phosphorylation on S473 by mTORC2 and on T308 by PDK-1.2,3 Akt can also be LMW-PTP/ACP1 Human/Mouse/Rat WB AF5075 100 µg $359 4 phosphorylated on S473 and T308 by IKKε. MIS/AMH Mouse/Rat IHC, WB AF1446 100 µg $359 Phosphorylation of Akt on S473 appears to play an important role in cancer. Inhibiting both the T308 Netrin-1 Human/Mouse/Rat WB AF6419 100 µg $359 and S473 sites is significantly more effective at Neuropilin-1 Mouse/Rat B/N, FC, IHC, WB AF566 100 µg $359 suppressing cancer cell proliferation than blocking Neuropilin-2 Mouse/Rat B/N, WB AF567 100 µg $359 the T308 site alone.5 This result implies that phosphorylation of Akt on S473 is a functionally Neuroplastin Mouse WB AF7818 100 µg $359 significant modification, even in the absence of Ninjurin-2 Human/Mouse/Rat IHC, WB AF5056 100 µg $359 phosphorylation on T308. Furthermore, phosphoryl- ation of Akt on S473, along with phosphorylated NQO-2 Human WB AF7868 100 µg $359 mTOR and phosphorylated Estrogen Receptor α, p67phox Human WB AF7830 100 µg $359 may be useful as a biomarker for tamoxifen benefit Polypeptide GalNac Transferase 2/GALNT2 Human WB AF7507 100 µg $359 in breast cancer.6 Polypeptide GalNac Transferase 10/GALNT10 Human/Mouse IHC, WB AF7575 100 µg $359 R&D Systems now offers a PE-conjugated Mouse Anti- Human/Mouse Phospho-Akt (S473) Monoclonal PRMT1 Human/Mouse WB AF6016 100 µg $359 Antibody (Catalog # IC7794P) and an Alexa Fluor® RanGAP1 Human WB AF7834 100 µg $359 488-conjugated Mouse Anti-Human/Mouse Phospho- Akt (S473) Monoclonal Antibody (Catalog # IC7794G). RelA/NFκB p65 Human/Mouse ChIP, WB AF5078 100 µg $359 Please see page 6 for more details. For more Renin R Human/Mouse WB AF5716 100 µg $359 information, please visit our website at Ribosomal Protein S6/RPS6 Human/Mouse/Rat WB AF5436 100 µg $359 www.RnDSystems.com/IntracellularKinases. Semaphorin 3E Human/Mouse ICC, IHC, WB AF3239 100 µg $359

50 Semaphorin 4A Human WB AF4694 100 µg $359 Semaphorin 6B Human/Mouse IHC, WB AF2094 100 µg $359 40 Sirtuin 3/SIRT3 Human WB AF7488 100 µg $359 Slit3 Human/Mouse/Rat B/N, WB AF3629 100 µg $359 30 ST6 Gal Sialyltransferase 2/ST6GAL2 Human IHC, WB AF7747 100 µg $359 20 ST8 α-2,8-Sialyltransferase 8A/ST8SIA1 Human IHC, WB AF6716 100 µg $359

Relative Cell Number Cell Relative UTF1 Mouse FC, ICC, WB AF7819 100 µg $359 10 ZFP90 Human WB AF7870 100 µg $359

0 100 101 102 103 104 Monoclonal Antibodies Phospho-Akt (S473)-PE Antibody Species APPLICATION Catalog # Size Price Detection of Phospho-Akt (S473) by Flow Cytometry. Resting Jurkat human acute T cell leukemia cell line (open histogram) or Jurkat cells treated with 14-3-3ζ Human IHC, WB MAB2669 100 µg $245 100 nM of the phosphatase inhibitor Calyculin A (Catalog # 1336) for 30 minutes (filled histogram) were stained with a PE-conjugated Mouse Anti-Human/ A20/TNFAIP3 Human ICC MAB75981 100 µg $245 Mouse Phospho-Akt (S473) Pan Monoclonal Antibody (Catalog # IC7794P). Phospho-Akt (S473) Pan Specific Human/Mouse WB MAB887 100 µg $245 ALK-7 Human IHC MAB7749 100 µg $245 References ALK-7 Human FC MAB77491 100 µg $245 1. Hers, I. et al. (2011) Cell. Signal. 23:1515. Allergin-1 Human FC, ICC MAB7638 100 µg $245 2. Liao, Y. & M.C. Hung (2010) Am. J. Transl. Res. 2:19. α1-Microglobulin Human ICC, WB MAB7724 100 µg $245 3. Sarbassov, D.D. et al. (2005) Science 307:1098. 4. Guo, J.P. et al. (2011) J. Biol. Chem. 286:37389. α1-Microglobulin Rat WB MAB7720 100 µg $245 5. Najafov, A. et al. (2012) Biochem. J. 448:285. Annexin A1 Human/Mouse/Rat WB MAB37701 100 µg $245 6. Bostner, J. et al. (2013) Breast Cancer Res. Treat. 137:397. ARNT/HIF-1β Human ICC, WB MAB5630 100 µg $245 β-Galactosidase-1/GLB1 Human WB MAB6464 100 µg $245 β-TrCP1/BTRC Human ICC, WB MAB7675 100 µg $245 BoNT-E Heavy Chain C. botulinum WB MAB7135 100 µg $245

4 For research use only. Not for use in diagnostic procedures. Monoclonal Antibodies PD-1 Programmed Death-1 (PD-1) is a type I trans- Antibody Species APPLICATION Catalog # Size Price membrane protein member of the CD28/CTLA-4 1,2 Carbohydrate Sulfotransferase 3/CHST3 Human IHC MAB7450 100 µg $245 subfamily of the immunoglobulin superfamily. PD-1 has a V-type immunoglobulin extracellular CCDC134 Human WB MAB7784 100 µg $245 domain that binds to PD-L1 or PD-L2, which are CCDC134 Mouse WB MAB7786 100 µg $245 members of the B7 family of immune regulatory ligands.3,4 Activation of PD-1 leads to inhibition of CD302/CLEC13A Human FC MAB6367 100 µg $245 T cell receptor- and B cell receptor-mediated signaling.5,6 PD-1 knockout mice display abnormal Connexin 43/GJA1 Human FC MAB7737 100 µg $245 B cell and T cell activity and experience early onset Cyclophilin A Human ICC, WB MAB3589 100 µg $245 of multiple autoimmune disorders including arthritis, lupus-like glomerulonephritis, and auto- Cytokeratin 18 Human ICC, WB MAB7619 100 µg $245 immune-associated dilated cardiomyopathy.5,6 Desmocollin-3 Mouse IHC, WB MAB7265 100 µg $245 R&D Systems now offers Alexa Fluor® 488-, APC-, Endophilin B1/B2 Human IHC, WB MAB7456 100 µg $245 and PE-conjugated Rat Anti-Mouse PD-1 Monoclonal Antibodies (Catalog # FAB7738G, FAB7738A, and EphB3 Human FC MAB56671 100 µg $245 FAB7738P, respectively). For more information on ETV2/ER71 Mouse WB MAB7740 100 µg $245 additional products for immune co-stimulatory research, please visit our website at FABP4/A-FABP Human/Mouse WB MAB3150 100 µg $245 www.RnDSystems.com/Costimulation. FBXW7/Cdc4 Human WB MAB7776 100 µg $245 Gephyrin/GPHN Human/Mouse WB MAB7519 100 µg $245 A. 80 Gi24/Dies1/VISTA Mouse ICC, WB MAB70051 100 µg $245 70 Glucosylceramidase/GBA Human ICC, WB MAB7410 100 µg $245 60 Glutathione S-Transferase pi 1/GSTP1 Human ICC, WB MAB6455 100 µg $245 50 Hemoglobin ζ Human ICC, WB MAB7708 100 µg $245 40 Histone Deacetylase 2/HDAC2 Human/Rat WB MAB7679 100 µg $245 30 IGSF4B/SynCAM3 Human WB MAB36781 100 µg $245 Relative Cell Number Cell Relative 20 IRF8 Human IHC, WB MAB5117 100 µg $245 10 KAP1 Human WB MAB7785 100 µg $245 0 KIR3DL2/CD158k Human FC MAB2878 100 µg $245 100 101 102 103 104 Kynureninase Mouse IHC, WB MAB7389 100 µg $245 PD-1

Latrophilin 3/LPHN3 Human/Mouse/Rat WB MAB5916 100 µg $245 B. 120 LEDGF Human ICC, WB MAB3468 100 µg $245 100 LMP2/PSMB9 Human ICC, WB MAB7709 100 µg $245 LRIG3 Human FC, WB MAB3495 100 µg $245 80

LRRC32/GARP Mouse FC MAB62291 100 µg $245 60 M-Cadherin/Cadherin-15 Mouse ICC MAB7677 100 µg $245 40

M-Cadherin/Cadherin-15 Mouse/Rat WB MAB76771 100 µg $245 Number Cell Relative MIF Mouse WB MAB1978 100 µg $245 10

MYF-5 Human ICC MAB4027 100 µg $245 0 0 1 2 3 4 NAALADase-2/NAALAD2 Human IHC, WB MAB7658 100 µg $245 10 10 10 10 10 PD-1 NAALADase-like 2/NAALADL2 Human FC MAB4665 100 µg $245 Detection of PD-1 by Flow Cytometry. Mouse splenocytes, A) resting or B) PAUF/ZG16B Human IHC, WB MAB7777 100 µg $245 treated with phytohaemagglutinin (PHA), were stained with a PE-conjugated Rat Anti-Mouse PD-1 Monoclonal Antibody (Catalog # FAB7738P; filled PCK1 Human WB MAB7316 100 µg $245 histograms) or a PE-conjugated Rat IgG2A Isotype Control (Catalog # IC006P; PD-1 Mouse FC MAB7738 100 µg $245 open histograms). PDZD2 Human ICC, WB MAB7670 100 µg $245 Perilipin-3 Human WB MAB7664 100 µg $245 References 1. Ishida, Y. et al. (1992) EMBO J. 11:3887. Perilipin-3 Human IHC, WB MAB76641 100 µg $245 2. Peggs, K.S. & J.P. Allison (2005) Br. J. Haematol. 130:809. PI 3-Kinase p55γ Human/Mouse/Rat WB MAB6638 100 µg $245 3. Zhang, X. et al. (2004) Immunity 20:337. pIgR Human WB MAB2717 100 µg $245 4. Collins, M. et al. (2005) Genome Biol. 6:223. 5. Sharpe, A.H. & G.J. Freeman (2002) Nat. Rev. Immunol. Plexin A2 Human/Mouse ICC, WB MAB5486 100 µg $245 2:116. Profilin 1 Human WB MAB7779 100 µg $245 6. Saresella, M. et al. (2012) Curr. Mol. Med. 12:259. Continued on page 6.

www.RnDSystems.com 5 c-Myc Monoclonal Antibodies v-myc Myelocytomatosis Viral Oncogene Homolog Antibody Species APPLICATION Catalog # Size Price (Avian) (c-Myc) is a member of the basic helix-loop- helix leucine zipper (bHLH-Zip) family of transcription Continued from page 5. factors and is known to regulate the expression of Protein S/PROS1 Mouse WB MAB4976 100 µg $245 cellular proliferation and differentiation genes. To facilitate transcriptional activation at E box sites, PSCA Human IHC MAB7636 100 µg $245 c-Myc forms a heterodimer with Max and recruits RFX6 Human WB MAB7780 100 µg $245 chromatin remodeling factors.1 c-Myc is an important Secretin R Human WB MAB6387 100 µg $245 regulator of pluripotency genes in embryonic stem cells, and exogenous expression of c-Myc and other Semaphorin 3C Human ICC MAB5570 100 µg $245 factors is sufficient to reprogram somatic cells to a Semaphorin 3C Human/Mouse ICC, WB MAB1728 500 µg $329 pluripotent state.2,3 c-Myc is a proto-oncogene that is frequently mutated and/or overexpressed in many Snapin Human/Mouse WB MAB7795 100 µg $245 cancers.4 Paradoxically, c-Myc can also function as an SOX6 Human ICC MAB7759 100 µg $245 apoptotic protein, although the mechanism TAZ/WWTR1 Human ICC, WB MAB7210 100 µg $245 regulating this functional switch remains unclear.5 TDP-43/TARDBP Human WB MAB7778 100 µg $245 R&D Systems now offers Alexa Fluor® 488- and APC- conjugated Mouse Anti-Human c-Myc Monoclonal TGIF1 Human WB MAB7555 100 µg $245 Antibodies (Catalog # IC3696G and IC3696A, TINAGL1 Human WB MAB7185 100 µg $245 respectively). For more information on other products for transcriptional regulation, please visit our website at Tissue α-L-Fucosidase/FUCA1 Human WB MAB7039 100 µg $245 www.RnDSystems.com/TranscriptionFactors. TLR11 Mouse FC MAB7640 100 µg $245

A. 30 VSIG2 Mouse IHC MAB4766 100 µg $245 Wnt-10b Human WB MAB7196 100 µg $245 25

20

15 Fluorochrome-labeled Antibodies Antibody Species Label Catalog # Size Price 10

Relative Cell Number Cell Relative Phospho-Akt (S473) Human/Mouse Alexa Fluor 488 IC7794G 100 Tests $299 5 Phospho-Akt (S473) Human/Mouse Phycoerythrin IC7794P 100 Tests $305

0 ALK-7 Human Allophycocyanin FAB77491A 100 Tests $349 100 101 102 103 104 Arginase 1/ARG1 Human/Mouse Fluorescein IC5868F 100 Tests $355 c-Myc-APC Phospho-Axl (Y779) Human Alexa Fluor 488 IC6965G 100 Tests $299 B. 40 B7-H1/PD-L1 Human PerCP FAB1561C 100 Tests $329 B7-H1/PD-L1 Human Alexa Fluor 700 FAB1561N 100 Tests $299 30 β-III Tubulin (TUJ-1) Multi-species NorthernLights™ 493 NL1195G 0.5 mL $349 E-Cadherin Human PerCP FAB18381C 100 Tests $329 20 CCL3/MIP-1α Mouse PerCP IC450C 100 Tests $329 CCR2 Human Alexa Fluor 700 FAB151N 100 Tests $299 Relative Cell Number Cell Relative 10 CD90/Thy1 Mouse Allophycocyanin FAB7335A 100 Tests $349 CD90/Thy1 Mouse Alexa Fluor 488 FAB7335G 100 Tests $299 0 100 101 102 103 104 CD90/Thy1 Mouse Phycoerythrin FAB7335P 100 Tests $305 c-Myc-Alexa Fluor 488 CD160 Human Alexa Fluor 700 FAB6700N 100 Tests $299 Detection of c-Myc by Flow Cytometry. The Jurkat human acute T cell leukemia CEACAM-1/CD66a Mouse Allophycocyanin FAB6480A 100 Tests $349 cell line was stained with an Alexa Fluor488-conjugated Mouse Anti-Human c-Myc Monoclonal Antibody (Catalog # IC3696G; filled histogram) or an Alexa Fluor CEACAM-1/CD66a Mouse Alexa Fluor 488 FAB6480G 100 Tests $299

488-conjugated Mouse IgG1 Isotype Control (Catalog # IC002G; open histogram) (A). In addition, Jurkat cells were stained with an APC-conjugated Mouse Anti- CEACAM-1/CD66a Mouse Phycoerythrin FAB6480P 100 Tests $305 Human c-Myc Monoclonal Antibody (Catalog # IC3696A; filled histogram) or APC- c-Myc Human Allophycocyanin IC3696A 100 Tests $349 conjugated Mouse IgG Isotype Control (Catalog # IC002A; open histogram) (B). 1 c-Myc Human Alexa Fluor 488 IC3696G 100 Tests $299 References Connexin 43/GJA1 Human Allophycocyanin FAB7737A 100 Tests $349 1. Grandori, C. et al. (2000) Annu. Rev. Cell Dev. Biol. 16:653. CX3CL1/Fractalkine Human Allophycocyanin IC365A 100 Tests $349 2. Cartwright, P. et al. (2005) Development 132:885. CXCL12/SDF-1 Human/Mouse Phycoerythrin IC350P 100 Tests $305 3. Takahashi, K. & S. Yamanaka (2006) Cell 126:663. CXCL13/BLC/BCA-1 Human Alexa Fluor 700 IC801N 100 Tests $299 4. Pelengaris, S. et al. (2002) Nat. Rev. Cancer 2:764. 5. Zhang, Q. et al. (2013) Proc. Natl. Acad. Sci. USA 110:978.

6 For research use only. Not for use in diagnostic procedures. Fluorochrome-labeled Antibodies MMP-14/MT1-MMP Matrix Metalloproteinase-14, (MMP-14), also known as Antibody Species Label Catalog # Size Price MT1-MMP, is a zinc- and calcium-dependent CXCR1/IL-8 RA Human PerCP FAB330C 100 Tests $329 endopeptidase that belongs to the matrix metallo­ 1 CXCR1/IL-8 RA Human Alexa Fluor 700 FAB330N 100 Tests $299 proteinase (MMP) family. Members of the MMP family are typically synthesized as inactive zymogens CXCR2/IL-8 RB Human Alexa Fluor 700 FAB331N 100 Tests $299 that, upon activation, degrade extracellular matrix CXCR3 Human Alexa Fluor 700 FAB160N 100 Tests $299 proteins. With few exceptions, MMPs share a core structure that includes a propeptide, a catalytic EphB3 Human Phycoerythrin FAB56671P 100 Tests $305 metalloproteinase domain, a linker peptide, and a Phospho-ERK1 (T202/Y204)/ Human/Mouse/Rat Alexa Fluor 488 IC7806G 100 Tests $299 hemopexin domain.2,3 In addition to the core MMP ERK2 (T185/Y187) structure, MMP-14 and other members of the FSH R Human Allophycocyanin FAB65591A 100 Tests $349 membrane type MMP (MT-MMP) subfamily have either a transmembrane domain (MMP-14, -15, -16, FSH R Human Phycoerythrin FAB65591P 100 Tests $305 and -24) or a glycosyl-phosphatidylinositol linkage Glycophorin A Human Allophycocyanin FAB12281A 100 Tests $349 (MMP-17 and MMP-25) which localizes MT-MMPs to 2,3 Glycophorin A Human PerCP FAB12281C 100 Tests $329 the cell surface. MMPs participate in normal biological processes such as tissue remodeling but HLA-DR Human Alexa Fluor 700 FAB4869N 100 Tests $299 have also been implicated in pathological processes HMGB1/HMG-1 Human Allophycocyanin IC1690A 100 Tests $349 such as preeclampsia and cancer metastasis.1,4,5 MMP-14 expression is upregulated in a number of HMGB1/HMG-1 Human Alexa Fluor 488 IC1690G 100 Tests $299 cancers and has been correlated with a poor IFN-α/β R1 Human Alexa Fluor 700 FAB245N 100 Tests $299 prognosis for gliomas, gastric cancer, and breast 5-7 IL-13 Rα1 Human Alexa Fluor 700 FAB1462N 100 Tests $299 cancer. IL-17 R Human Alexa Fluor 700 FAB177N 100 Tests $299 R&D Systems now offers Alexa Fluor® 488-, Alexa Fluor® 700-, APC-, PE-, and PerCP-conjugated Mouse Anti- KIR2DL3/CD158b2 Human PerCP FAB2014C 100 Tests $329 Human MMP-14 Monoclonal Antibodies (Catalog KIR3DL2/CD158k Human Allophycocyanin FAB2878A 100 Tests $349 # FAB9181G, FAB9181N, FAB9181A, FAB9181P, and FAB9181C, respectively). For more information on LRIG3 Human Allophycocyanin FAB3495A 100 Tests $349 products for MMP research, please visit our website at MICL/CLEC12A Human PerCP FAB2946C 100 Tests $329 www.RnDSystems.com/MMP. MICL/CLEC12A Human Alexa Fluor 488 FAB2946G 100 Tests $299 70 MICL/CLEC12A Human Alexa Fluor 700 FAB2946N 100 Tests $299 60 MMP-14/MT1-MMP Human Alexa Fluor 700 FAB9181N 100 Tests $299 50 MS4A4A Human Phycoerythrin FAB7797P 100 Tests $305 40 MUC-1 Human Alexa Fluor 488 FAB6298G 100 Tests $299 MUC-1 Human Phycoerythrin FAB6298P 100 Tests $305 30

NAALADase-like 2/NAALADL2 Human Allophycocyanin FAB4665A 100 Tests $349 20 Relative Cell Number Cell Relative

NAALADase-like 2/NAALADL2 Human Alexa Fluor 488 FAB4665G 100 Tests $299 10 NAALADase-like 2/NAALADL2 Human Phycoerythrin FAB4665P 100 Tests $305 0 Nanog Human NorthernLights 557 NL1997R 0.5 mL $355 100 101 102 103 104 MMP-14 NCAM-1/CD56 Mouse Allophycocyanin FAB7820A 100 Tests $349 NCAM-1/CD56 Mouse Alexa Fluor 488 FAB7820G 100 Tests $299 Detection of MMP-14/MT1-MMP by Flow Cytometry. The MDA-MB-231 human breast cancer cell line was stained with an Alexa Fluor® 700-conjugated Mouse NCAM-1/CD56 Mouse Phycoerythrin FAB7820P 100 Tests $305 Anti-Human MMP-14/MT1-MMP Antibody (Catalog # FAB9181N; filled histogram) or an Alexa Fluor® 700-conjugated Mouse IgG Isotype Control (Catalog Nestin Rat NorthernLights 493 NL27361G 0.5 mL $355 2B # IC0041N; open histogram). Nestin Rat NorthernLights 557 NL27361R 0.5 mL $355 NK1R Human Allophycocyanin FAB66871A 100 Tests $349 References Oct-4A Human NorthernLights 493 NL17591G 0.5 mL $349 1. Vu, T.H. & Z. Werb (2000) Genes Dev. 14:2123. Oct-4A Human NorthernLights 557 NL17591R 0.5 mL $349 2. Itoh, Y. et al. (1999) J. Biol. Chem. 274:34260. Oct-4A Human NorthernLights 637 NL17591V 0.5 mL $349 3. Kojima, S. et al. (2000) FEBS Lett. 480:142. 4. Kaitu’u-Lino, T.J. et al. (2012) Am. J. Pathol. 180:888. Oligodendrocyte Marker O4 Human/Mouse/Rat/ NorthernLights 493 NL1326G 0.5 mL $349 5. Têtu, B. et al. (2006) Breast Cancer Res. 8:R28. Chicken 6. He, L. et al. (2013) Dig. Dis. Sci. in press. Otx2 Human NorthernLights 493 NL1979G 0.5 mL $355 7. Wang, L. et al. (2013) Clin. Transl. Oncol. 15:139. PD-1 Mouse Allophycocyanin FAB7738A 100 Tests $349 PD-1 Mouse Alexa Fluor 488 FAB7738G 100 Tests $299 Continued on page 8.

www.RnDSystems.com 7 GloLIVE™ Monoclonal Antibodies Fluorochrome-labeled Antibodies to Verify Pluripotency in Live Stem Cells Antibody Species Label Catalog # Size Price Maintaining pluripotent human embryonic stem cells and deriving induced pluripotent stem cells require Continued from page 7. colony selection and cell expansion. Picking colonies PD-1 Mouse Phycoerythrin FAB7738P 100 Tests $305 that contain pluripotent stem cells is typically achieved RAMP2 Mouse Phycoerythrin FAB6500P 100 Tests $305 by manually analyzing colony morphology. Unfortunately, this critical process is time-consuming ROR1 Human Alexa Fluor 488 FAB2000G 100 Tests $355 and labor-intensive. In addition, morphology-based SSEA-3 Human/Mouse Alexa Fluor 488 FAB1434G 100 Tests $299 colony selection does not consistently guarantee pluripotent starting populations. TLR3 Mouse Allophycocyanin IC3005A 100 Tests $349 To address this need, R&D Systems offers GloLIVE TLR4 Human PerCP FAB6248C 100 Tests $329 antibodies that allow researchers to confirm stem TLR4 Human Alexa Fluor 700 FAB6248N 100 Tests $299 cell marker expression in live cells before colony TREM-1 Human Allophycocyanin FAB1278A 100 Tests $349 selection. Quick and easy to use, GloLIVE antibodies are azide-free and conjugated to NorthernLights™ TREM-1 Human PerCP FAB1278C 100 Tests $329 (NL) fluorochromes. Importantly, incubation with TREM-1 Mouse PerCP FAB1187C 100 Tests $329 GloLIVE antibodies permits continued cell culture with no adverse affects on cell proliferation or TREM-1 Mouse Alexa Fluor 488 FAB1187G 100 Tests $299 stemness following the staining procedure. TREM-1 Mouse Alexa Fluor 700 FAB1187N 100 Tests $299 GloLIVE antibodies increase the probability of exper­ TREM-2 Human/Mouse Alexa Fluor 700 FAB17291N 100 Tests $299 imental success by allowing single-step staining of TSLP R Mouse Allophycocyanin FAB5461A 100 Tests $355 established pluripotency markers SSEA-1, SSEA-4, and TRA-1-60. SSEA-1 is a positive marker for mouse ULBP-2/5/6 Human PerCP FAB1298C 100 Tests $329 pluripotent stem cells and a negative marker for human pluripotent stem cells.1,2 In contrast, SSEA-4 is considered a positive marker of pluripotency in human stem cells and a negative marker in mouse stem cells.3,4 Detection of TRA-1-60 expression is specific to human pluripotent stem cells.5 GloLIVE Monoclonal Antibodies for Staining in Live Cells R&D Systems now offers Mouse Anti-Human/ R&D Systems offers antibodies conjugated to NorthernLights (NL) fluorochromes that can be used for Mouse SSEA-1 (Catalog #s NLLC2155G and NLLC2155R), single-step staining of pluripotency in cultured stem cells. Mouse Anti-Human/Mouse SSEA-4 (Catalog #s NLLC1435G and NLLC1435R), and Mouse Anti-Human ANTIBODY SPECIES Label CATALOG # SIZE PRICE TRA-1-60 Monoclonal Antibodies (Catalog #s SSEA-1 Human/Mouse NorthernLights 493 NLLC2155G 0.5 mL $455 NLLC4770G and NLLC4770R) conjugated to NL493 (green) and NL557 (red) fluorochromes. For more SSEA-1 Human/Mouse NorthernLights 557 NLLC2155R 0.5 mL $455 information on products for embryonic and induced SSEA-4 Human/Mouse NorthernLights 493 NLLC1435G 0.5 mL $455 pluripotent stem cells markers, please visit our website at www.RnDSystems.com/StemCells. SSEA-4 Human/Mouse NorthernLights 557 NLLC1435R 0.5 mL $455 TRA-1-60(R) Human NorthernLights 493 NLLC4770G 0.5 mL $455 TRA-1-60(R) Human NorthernLights 557 NLLC4770R 0.5 mL $455

Biotinylated Antibodies ANTIBODY SPECIES TYPE CATALOG # SIZE PRICE IL-5 Mouse/Rat Goat IgG BAF545 50 µg $390 Detection of SSEA-4 in Live BG01V Cells. The stem cell marker SSEA-4 was visualized in live BG01V human embryonic stem cells using the GloLIVE Mouse Anti-Human/Mouse SSEA-4-NL557 Monoclonal Antibody (Catalog # NLLC1435R, red). The cells were counterstained with Hoechst 33342 (blue). Positive staining for SSEA-4 in human cells is an indicator of pluripotency. References Secondary Antibodies 1. Solter, D. & B.B. Knowles (1978) Proc. Natl. Acad. Sci. USA ANTIBODY SPECIES LABEL CATALOG # SIZE PRICE 75:5565. IgG Rabbit Alexa Fluor 488 IC105G 200 Tests $169 2. Fox, N. et al. (1983) Cancer Res. 43:669. 3. Shevinsky, L.H. et al. (1982) Cell 30:697. 4. Draper, J.S. et al. (2002) J. Anat. 200:249. 5. Andrews, P. et al. (1984) Hybridoma 3:347.

8 For research use only. Not for use in diagnostic procedures. DuoSet® ELISA Development Systems TRANCE/RANK Ligand DuoSet ELISA TRANCE/RANK Ligand is a single-pass type II AnALYTE Species Catalog # Reagents for* Price transmembrane protein belonging to the tumor α1-Acid Glycoprotein Human DY3694 15 Plates $649 necrosis factor superfamily. It is expressed on the ADAMTS1 Human DY2197 15 Plates $649 surface of osteoblasts and regulates bone resorption by binding RANK/TNFRSF11A on osteoclasts.1-3 The Aminopeptidase N/ANPEP Human DY3815 15 Plates $649 soluble decoy receptor Osteoprotegerin/TNFRSF11B BMP-9 Mouse DY5566 15 Plates $649 also binds to TRANCE/RANK Ligand, which counterbalances the ability of TRANCE/RANK Ligand CD163 Mouse DY7435 15 Plates $649 to activate RANK.4 In addition to regulating bone Complement Factor H Human DY4779 15 Plates $649 metabolism, TRANCE/RANK Ligand mediates 5 Endothelin Pan Specific Multi-species DY1160 15 Plates $649 immune system activation. A soluble or transmembrane form of TRANCE/RANK Ligand Granulysin Human DY3138 15 Plates $649 expressed by T cells promotes dendritic cell HGF Activator Human DY1514 15 Plates $649 stimulation of naïve T cells.6 Additionally, expression of TRANCE/RANK Ligand by activated T cells is IFN-γ Porcine DY985 15 Plates $649 thought to induce bone loss, leading to joint IL-1F7/FIL1ζ Human DY1975 15 Plates $649 destruction and adjuvant arthritis.7 Overexpression IL-1 RAcP/IL-1 R3 Human DY676 15 Plates $649 of TRANCE/RANK Ligand also contributes to the pathogenesis of degenerative bone disorders such IL-4 Canine DY754 15 Plates $649 as osteoporosis and Paget’s Disease.8,9 Like IL-9 Mouse DY409 15 Plates $649 osteoblasts and T cells, breast and prostate cancer cells and multiple myeloma cells express TRANCE/ LRIG3 Human DY3495 15 Plates $649 RANK Ligand, which likely enhances the ability of MIS/AMH Human DY1737 15 Plates $649 these cancers to metastasize to bone.10 As a result, Nesfatin-1/Nucleobindin-2 Human DY5949 15 Plates $649 TRANCE/RANK Ligand signaling is a therapeutic target for inhibiting the progression of degenerative Proprotein Convertase 9/PCSK9 Mouse DY3985 15 Plates $649 bone disorders, cancer-associated bone loss, and R-Spondin 3 Human DY3500 15 Plates $649 cancer metastasis to bone.11-13 S100A9 Human DY5578 15 Plates $649 R&D Systems now offers a Human TRANCE/RANK Ligand DuoSet ELISA Development System (Catalog # Serpin A1/α1-Antitrypsin Human DY1268 15 Plates $649 DY626). For more information on products for Syndecan-3 Human DY3539 15 Plates $649 bone-related markers, please visit our website at Syndecan-4 Human DY2918 15 Plates $649 www.RnDSystems.com/BoneMarkers. TRANCE/TNFSF11/RANK L Human DY626 15 Plates $649 Osteoclast Precursor *Also available in 45 plate Economy Packs for $1395.

RANK DuoSet® IC (Intracellular) ELISA Development Systems TRANCE/RANK Ligand AnALYTE Species Catalog # Reagents for* Price Phospho-Histone H2AX (S139) Human DYC2288-2 2 Plates $329 Osteoblast *Also available in 5 plate packs for $649 and 15 plate Economy Packs for $1395. Activated Osteoclast

Bone Resorption bone

ELISpot Kits & Development Modules References For the detection and the quantitation of analyte-specific secreting cells. 1. Lacey, D.L. et al. (1998) Cell 93:165. 2. Nakagawa, N. et al. (1998) Biochem. Biophys. Res product Species Catalog # size Price Commun. 253:395. B Cell ELISpot Development Module Mouse SELB004 1 Kit $465 3. Fuller, K. et al. (1998) J. Exp. Med. 188:997. B Cell ELISpot Development Module Mouse SELB005 1 Kit $465 4. Zaidi, M. (2007) Nat. Med. 13:791. 5. Akiyama, T. et al. (2012) World J. Orthop. 3:142. IFN-γ/IL-2 Dual Color ELISpot Kit Primate ELD7595 1 Kit $555 6. Anderson, D.M. et al. (1997) Nature 390:175. IL-2 Development Module Primate SEL7594 1 Kit $465 7. Kong, Y.Y. et al. (1999) Nature 402:304. IL-12/IL-23 p40 ELISpot Kit Mouse EL499 1 Kit $445 8. Wada, T. et al. (2006) Trends Mol. Med. 12:17. IL-12/IL-23 p40 Development Module Mouse SEL499 1 Kit $465 9. Goessl, C. et al. (2012) Ann. N. Y. Acad. Sci. 1263:29. 10. Lipton, A. & I. Jacobs (2011) Curr. Opin. Support Palliat. TNF-α Development Module Rat SEL510 1 Kit $465 Care 5:258. 11. Ford, J.A. et al. (2013) Eur. J. Cancer 49:416. 12. Yee, A.J. & N.S. Raje (2012) Clin. Interv. Aging 7:331. 13. Sutton, E.E. & D.M. Riche (2012) Ann. Pharmacother. 46:1000. www.RnDSystems.com 9 Proteome Profiler™ Human Protease Proteome Profiler™ Antibody Arrays Antibody Array product description Catalog # size price The Proteome Profiler Human Protease Array (Catalog # ARY021) provides a rapid, sensitive Human Protease Array Kit Contains 4 membranes - each spotted in ARY021 1 Kit $499 duplicate with antibodies against 34 method for simultaneously detecting the relative different proteases expression of 34 human proteases in a single sample of cell culture supernate, cell lysate, tissue lysate, ADAM8, ADAM9, ADAMTS1, ADAMTS13, Cathepsin A, Cathepsin B, Cathepsin C, Cathepsin D, Cathepsin E, Cathepsin L, Cathepsin S, Cathepsin V, Cathepsin X/Z/P, DPPIV/CD26, Kallikrein 3/PSA, Kallikrein 5, Kallikrein 6, Kallikrein 7, Kallikrein 10, Kallikrein 11, Kallikrein 13, MMP-1, MMP-2, MMP-3, MMP-7, MMP- serum, plasma, urine, or saliva. Proteome Profiler 8, MMP-9, MMP-12, MMP-13, Neprilysin/CD10, Presenilin-1, Proprotein Convertase 9, Proteinase 3, uPA/Urokinase arrays are nitrocellulose membrane-based and do not require any specialized equipment, making them an ideal alternative to performing multiple immunoprecipitation/Western blot experiments. For more details, please visit our website at www.RnDSystems.com/ProteaseArray. Luminex® Performance Assays Luminex Performace Assays are our most accurate and precise bead-based multianalyte profiling kits. Kits consist of base kits and bead sets, which can be ordered individually or as premixed kits. For more Features information, visit our website at www.RnDSystems.com/LuminexPerformance. ✓ Rapid screening of 34 proteases Rat Kidney Toxicity Assay Kit ✓ Easier to perform than a Western blot AnALYTE Species sensitivity range Catalog # size Price ✓ Requires no specialized equipment Premix Kit Rat NA NA FCST15 1 Kit Inquire ✓ Suitable for multiple sample types Base Kit Rat NA NA LRK000 1 Kit $219 Cystatin C Rat 11.8 pg/mL 26-19,000 pg/mL LRK6154 1 Kit $175 A. Untreated FABP1/L-FABP Rat 108 pg/mL 3,409-2,485,500 pg/mL LRK1565 1 Kit $175 ADAM9 Cathepsin S Cathepsin A Cathepsin D Lipocalin-2/NGAL Rat 3.8 pg/mL 8-5,900 pg/mL LRK3508 1 Kit $175 RS TIM-1/KIM-1/HAVCR Rat 2.6 pg/mL 14-10,170 pg/mL LRK3689 1 Kit $175 RS Osteopontin/OPN Rat 0.9 pg/mL 13-9,200 pg/mL LRK6359 1 Kit $175

MMP-12 MMP-13 MMP-2 MMP-3

B. IL-1α + OSM Treated ADAM9 Cathepsin S Cathepsin A Cathepsin D Fluorokine® Flow Cytometry Kits RS Employs biotinylated recombinant ligands to measure cytokine receptor levels using flow cytometry.

RS AnALYTE Species label Catalog # size Price Angiopoietin-1 Human Biotin NFANG1 1 Kit $405 MMP-12 MMP-13 MMP-2 MMP-3

C. Untreated vs IL-1α + OSM Treated 60 Untreated )

3 IL-1α + OSM Treated 50 Cell-Based ELISA Assay Kits 40 Cell-Based ELISA Assays simultaneously measure the levels of two proteins in the same microplate well using whole, fixed cells, thus eliminating the need for lysate preparation. These kits are ideal for 30 analyzing the levels of either a phospho-protein relative to total protein or a specific protein relative to a housekeeping protein. Normalizing the reference protein to the test protein corrects for well-to-well 20 variations and allows protein levels to be accurately assessed and compared across multiple samples.

10 Analyte Species Catalog # Size Price Mean Pixel Density (x10 Mean Pixel 0 Phospho-PRAS40 (T246) Human/Mouse KCB6890 1 Kit $455 Phospho-Smad1 (S463/S465)/ Human/Mouse KCB7660 1 Kit $455

ADAM9 MMP-1 MMP-3 MMP-12 MMP-13 Smad5 (S463/S465)

Cathepsin A Cathepsin D Cathepsin S

Simultaneous Detection of Multiple Proteases Following Treatment with IL-1α and Oncostatin M (OSM). The Proteome Profiler Human Protease Antibody Array (Catalog # ARY021) was used to simultaneously assess protease levels in cell lysates from the SW1353 human chondrosarcoma cell Stem Cell Products line. Cells were either untreated (A) or treated (B) with Recombinant Human IL-1α (Catalog # 200-LA) and Recombinant Human OSM (Catalog # 295-OM). MEDIA Species Catalog # size Price Chemiluminescence was detected using x-ray film and histogram profiles (C) StemXVivo™ Chondrogenic Supplement Rat CCM020 0.5 mL $129 for select analytes were generated by quantifying the mean spot pixel densities using standard imaging software.

10 For research use only. Not for use in diagnostic procedures. VersaClone™ cDNA Plasmids VersaClone™ cDNA Plasmids R&D Systems is now offering more than 500 Plasmid (10 µg) Species Catalog # Price Plasmid (10 µg) Species Catalog # Price VersaClone cDNA plasmids for the quick and 5-HT1A Human RDC0283 $199 Cysteinyl Leukotriene Human RDC0292 $199 efficient cloning of human, mouse, or rat genes R2/CysLTR2 5-HT1D Human RDC0285 $199 coding for transmembrane proteins. The Versa- Cysteinyl Leukotriene Mouse RDC0450 $199 Clone cDNA plasmids contain the entire open 5-HT1E Human RDC0286 $199 R2/CysLTR2 reading frame (ORF) for the gene of interest, 5-HT1F Human RDC0287 $199 δ Opioid R/OPRD1 Human RDC0326 $199 allowing users to express the full length, mature receptor. They also contain a Kozak consensus 5-HT2A Human RDC0288 $199 EBI2 Human RDC0219 $199 sequence upstream of the gene to drive protein 5-HT2B Human RDC0289 $199 EBI2 Mouse RDC0445 $199 expression and a unique multiple cloning site (MCS) region with numerous sites for staggered and blunt- 5-HT5A Human RDC0249 $199 EMP3 Human RDC0512 $199 end cleavage, making these plasmids compatible 5-HT7 Human RDC0252 $199 EMR4 Mouse RDC0208 $199 with most expression vectors. The sequence for Adenosine A1 R Human RDC0213 $199 F4/80/EMR1 Human RDC0528 $199 each VersaClone cDNA plasmid is verified against reference sequences in the National Center for Adenosine A2a R Human RDC0214 $199 F4/80/EMR1 Human RDC0538 $199 Biotechnology Information (NCBI) Gene database. Adenosine A2b R Human RDC0215 $199 Fcε RIβ/MS4A2 Human RDC0229 $199 For a current list of available VersaClone cDNA Adenosine A3 R Human RDC0216 $199 Fcε RIβ/MS4A2 Mouse RDC0230 $199 plasmids, please visit our website at www.RnDSystems.com/VersaClone. Adenosine A3 R Human RDC0217 $199 FFAR1/GPR40 Human RDC0272 $199 Adrenomedullin R/ Human RDC0223 $199 FFAR1/GPR40 Mouse RDC0516 $199 Features ADMR FFAR2/GPR43 Mouse RDC0526 $199 ✓ Highly purified Adrenomedullin R/ Mouse RDC0224 $199 ADMR FFAR3/GPR41 Mouse RDC0525 $199 ✓ Contains a Kozak consensus sequence and the full ORF for expression of the full-length protein α-1A Adrenergic R/ Human RDC0253 $199 FGF R4 Human RDC0536 $199 ADRA1A ✓ Sequence verified against the NCBI Gene database GABAA R ρ1 Human RDC0262 $199 α-1D Adrenergic R/ Human RDC0255 $199 ✓ GABAA R ρ2 Human RDC0271 $199 Contains an MCS with more restriction enzyme cleavage ADRA1D sites than most commercially available cDNA plasmids α GABAA R θ Human RDC0218 $199 -2A Adrenergic R/ Human RDC0256 $199 ✓ Compatible with most expression vectors ADRA2A Galanin R2 Human RDC0470 $199 α-2B Adrenergic R/ Human RDC0257 $199 ADRA2B Glucagon R/GLR Human RDC0323 $199 BmgBI HpaI α GluR7/GRIK3 Human RDC0266 $199 HindIII -2C Adrenergic R/ Human RDC0258 $199 BamHI ADRA2C GnRHR Human RDC0324 $199 Pfol BstAPI EcoRV SspI AatII Ndel Nhel β-1 Adrenergic R/ Human RDC0259 $199 GnRHR Mouse RDC0415 $199 Bbel NotI ADRB1 XmnI BstXI EagI GPR1 Human RDC0296 $199 ScaI NcoI β-2 Adrenergic R/ Human RDC0260 $199 Sfil ADRB2 GPR4 Human RDC0299 $199 BsrGI β-3 Adrenergic R/ Human RDC0261 $199 GPR4 Human RDC0300 $199 AMP BgIII NmeAIII Acc65I ADRB3 hCCR1 KpnI GPR6 Human RDC0301 $199 BsrFI DraIII BasI (1-355) BRS3 Human RDC0278 $199 3815 bps RsrII GPR10 Human RDC0302 $199 AhdI AarI Calcium-sensing R/ Human RDC0311 $199 PstI CaSR GPR17 Human RDC0304 $199 PsiI CCR1L1 Mouse RDC0207 $199 GPR18 Human RDC0305 $199 AccIII CD9 Mouse RDC0314 $199 GPR19 Human RDC0306 $199 COLE1 SexAI GPR20 Human RDC0307 $199 PspXI AscI CD36/SR-B3 Human RDC0546 $199 XhoI BssHII PciI Eco47III Bst1107I CD97 Human RDC0547 $199 GPR25 Human RDC0310 $199 XbaI SaII Cholecystokinin-B R/ Human RDC0351 $199 GPR45 Human RDC0246 $199 SmaI CCKBR GPR55 Human RDC0247 $199 Map of a VersaClone cDNA Plasmid. The Human CCR1 VersaClone cDNA Claudin-18 Human RDC0298 $199 GPR78 Human RDC0269 $199 Plasmid (Catalog # RDC0001) contains the complete open reading frame for the Connexin 43/GJA1 Human RDC0534 $199 GPR120/O3FAR1 Human RDC0511 $199 human CCR1 gene. The plasmid also contains a Kozak consensus sequence for CRLR Human RDC0321 $199 optimal translation initiation. The gene was inserted using the NotI and AscI GPR177/WLS Human RDC0212 $199 restriction enzymes and is flanked with multiple cloning sites that can be used Cysteinyl Leukotriene Human RDC0291 $199 to easily cut and transfer the gene cassette into your desired expression vector. R1/CysLTR1 Histamine H2 R Human RDC0342 $199 Cysteinyl Leukotriene Mouse RDC0391 $199 Histamine H2 R Human RDC0343 $199 R1/CysLTR1 Continued on page 12.

www.RnDSystems.com 11 Free IGF-I ELISA VersaClone™ cDNA Plasmids Insulin-like Growth Factor-I (IGF-I), also known as Somatomedin C, is a peptide growth hormone with a Plasmid (10 µg) Species Catalog # Price Plasmid (10 µg) Species Catalog # Price structure similar to Insulin.1 IGF-I is primarily Continued from page 11. P2Y1/P2RY1 Human RDC0334 $199 produced and secreted by hepatocytes in response Histamine H2 R Mouse RDC0393 $199 P2Y4/P2RY4 Human RDC0335 $199 to growth hormone, but it is also produced by many other cell types and can act in an autocrine or Histamine H3 R Human RDC0344 $199 P2Y6/P2RY6 Human RDC0336 $199 paracrine manner.2,3 IGF-I is synthesized as a Histamine H4 R Human RDC0345 $199 P2Y10/P2RY10 Human RDC0337 $199 prohormone that is processed into the mature, KA2/GRIK5 Human RDC0375 $199 bioactive hormone by endoproteolytic removal of P2Y11/P2RY11 Human RDC0338 $199 the C-terminus.4 It binds to two cell surface receptors, KIR3DS1/CD158e2 Human RDC0519 $199 P2Y12/P2RY12 Human RDC0339 $199 the type I IGF receptor (IGF-I R), which mediates the Lgr6 Mouse RDC0332 $199 P2Y13/P2RY13 Human RDC0340 $199 effects of IGF-I, and the type II IGF receptor (IGF-II R), which may not signal.5 IGF-I also binds to at least six LPAR3/LPA3/EDG-7 Mouse RDC0561 $199 P2Y14/GPR105 Human RDC0378 $199 soluble binding proteins, IGFBP-1 through IGFBP-6, LPAR1/LPA1/EDG-2 Mouse RDC0565 $199 Prokineticin R2/ Human RDC0172 $199 which bind the majority of circulating IGF-I.5 While PROKR2 Melatonin R1A/MT1/ Human RDC0147 $199 bound IGF-I is stabilized, increasing the half life of the MTNR1A PTGDR Human RDC0240 $199 protein from minutes to hours, it is not bioavailable.6 Serum levels of IGF-I increase from birth through Melatonin R1B/MT2/ Human RDC0148 $199 PTGFR Human RDC0267 $199 MTNR1B puberty, then slowly decline throughout adulthood.7 PTGIR Human RDC0268 $199 IGF-I mediates multiple physiological processes Motilin R/GPR38 Human RDC0244 $199 SLC12A3 Human RDC0558 $199 including prenatal development, growth, MS4A4C Mouse RDC0235 $199 metabolism, and protection of cardiomyocytes, STAMP2 Human RDC0548 $199 hepatocytes, and neurons against apoptosis.7 IGF-I MS4A5 Human RDC0236 $199 STEAP1 Human RDC0559 $199 deficiency is linked to Laron Syndrome, liver cirrhosis, MS4A5 Mouse RDC0237 $199 and age-associated disorders such as hypertension, TCIRG1 Human RDC0186 $199 MS4A6A Human RDC0386 $199 neurodegeneration, osteopenia, and sarcopenia.7 Thromboxane A2 R/ Human RDC0185 $199 IGF-I has been implicated in the development or MS4A7 Human RDC0348 $199 TBXA2R progression of most solid tumors and hematological MS4A8B Human RDC0349 $199 TM4SF4 Human RDC0187 $199 malignancies and is a current therapeutic target for many cancers.8 Serum levels of IGF-I are indicative of MS4A10 Human RDC0239 $199 TMEM18 Human RDC0226 $199 several diseases including acromegaly, Alzheimer’s µ Opioid R/OPMR1 Human RDC0530 $199 TrkC Human RDC0555 $199 disease, diabetes, hypertension, and osteoporosis.9-13 Neuromedin U R1/ Human RDC0152 $199 TRPM8 Human RDC0188 $199 R&D Systems now offers a Human Free IGF-I NMUR1 TSPAN1 Human RDC0190 $199 ELISA Kit (Catalog # DFG100). For more infor- Neuromedin U R2/ Human RDC0153 $199 mation on IGF-I-related products, please visit our NMUR2 TSPAN2 Human RDC0191 $199 website at www.RnDSystems.com/IGFFamily. Nicotinic Acetylcholine Human RDC0279 $199 TSPAN3 Human RDC0192 $199 R α1/CHRNA1 TSPAN6 Human RDC0193 $199 References Nicotinic Acetylcholine Human RDC0280 $199 R α1/CHRNA1 TSPAN7 Human RDC0194 $199 1. Rinderknecht, E. & R.E. Humbel (1978) J. Biol. Chem. 253:2769. Nicotinic Acetylcholine Human RDC0281 $199 TSPAN10 Human RDC0197 $199 α 2. Daughaday, W.H. & P. Rotwein (1989) Endocr. Rev. 10:68. R 4/CHRNA4 TSPAN12 Human RDC0198 $199 3. Yakar, S. et al. (1999) Proc. Natl. Acad. Sci. USA 96:7324. Nicotinic Acetylcholine Human RDC0282 $199 TSPAN15 Human RDC0199 $199 4. Duguay, S.J. et al. (1995) J. Biol. Chem. 270:17566. R a7/CHRNA7 TSPAN19 Human RDC0200 $199 5. Grimberg, A. & P. Cohen (2000) J. Cell. Physiol. 183:1. NK3R/TACR3 Human RDC0184 $199 Urotensin-II R Human RDC0201 $199 6. Guler, H.P. et al. (1989) Acta Endocrinol. (Copenh) OATP1B1/OATP2 Human RDC0160 $199 121:753. Urotensin-II R Mouse RDC0202 $199 7. Puche, J.E. & I. Castilla-Cortazar (2012) J. Transl. Med. OGR1 Human RDC0162 $199 10:224. V1a Vasopressin R/ Human RDC0273 $199 Orexin R2/HCRTR2 Human RDC0522 $199 AVPR1A 8. Weroha, S.J. & P. Haluska (2012) Endocrinol. Metab. Clin. North Am. 41:335. ORL1/OPRL1 Human RDC0163 $199 V1b Vasopressin R/ Human RDC0274 $199 9. Teppala, S. & A. Shankar (2010) Diabetes Care 33:2257. OX40/TNFRSF4 Rat RDC0361 $199 AVPR1B 10. Liu, J.M. et al. (2008) J. Bone Miner. Metab. 26:159. OXGR1/GPR80 Human RDC0270 $199 V2 Vasopressin R/ Human RDC0275 $199 11. Colao, A. et al. (2008) Eur. J. Endocrinol. 159:389. AVPR2 P2Y1/P2RY1 Human RDC0333 $199 12. Duron, E. et al. (2012) J. Clin. Endocrinol. Metab. VIGR/GPR126 Human RDC0203 $199 97:4673. 13. Brabant, G. (2003) Eur. J. Endocrinol. 148(Suppl 2):S15.

12 For research use only. Not for use in diagnostic procedures. Biochemicals & Compounds target Compound name Description Catalog # Size Price 17b-Hydroxysteroid Dehydrogenase Type 3 TC HSD 21 Potent and selective 17β-Hydroxysteroid Dehydrogenase Type 3 inhibitor 4575/10 10 mg $145 17b-Hydroxysteroid Dehydrogenase Type 3 TC HSD 21 Potent and selective 17β-Hydroxysteroid Dehydrogenase Type 3 inhibitor 4575/50 50 mg $599 a3b4 Nicotinic Acetylcholine Receptor (a3b4 nAChR) SR 16584 Selective α3β4 nAChR antagonist 4424/10 10 mg $179 α3β4 Nicotinic Acetylcholine Receptor (α3β4 nAChR) SR 16584 Selective α3β4 nAChR antagonist 4424/50 50 mg $735 Adrenergicβ2 Receptor Salmeterol xinafoate Long-acting Adrenergic β2 Receptor agonist; bronchodilator 4712/10 10 mg $119 Adrenergicβ2 Receptor Salmeterol xinafoate Long-acting Adrenergic β2 Receptor agonist; bronchodilator 4712/50 50 mg $509 Aldehyde Dehydrogenase 1a2 (ALDH1a2) WIN 18446 ALDH1a2 inhibitor 4736/50 50 mg $99 ATP Citrate Lyase (ACL) BMS 303141 Orally bioavailable ACL inhibitor 4609/10 10 mg $159 ATP Citrate Lyase (ACL) BMS 303141 Orally bioavailable ACL inhibitor 4609/50 50 mg $679 β-Catenin JW 67 Induces degradation of active β-catenin; inhibits the Wnt pathway 4651/10 10 mg $129 β-Catenin JW 67 Induces degradation of active β-catenin; inhibits the Wnt pathway 4651/50 50 mg $575 Breast Cancer Resistance Protein (BCRP) Fumitremorgin C Inhibitor of BCRP-mediated multidrug resistance 2876/250U 250 µg $179 Ca2+ Channel (Stretch-activated) Gadolinium chloride Stretch-activated Ca2+ channel blocker; Ca2+-sensing Receptor agonist 4741/50 50 mg $39 Cav3.1 Ca2+ Channel ProTx I Cav3.1 Ca2+ channel blocker; inhibits Nav1.8 Na+ and Kv2.1 K+ channels 4665/100U 100 µg $189 Cannabinoid CB1 Receptor SR 141716A Selective CB1 Receptor antagonist 0923/10 10 mg $95 Cannabinoid CB1 Receptor SR 141716A Selective CB1 Receptor antagonist 0923/50 50 mg $375 Catechol O-Methyltransferase (COMT) Entacapone Potent COMT inhibitor; blocks α-Synuclein aggregation 4720/10 10 mg $49 Catechol O-Methyltransferase (COMT) Entacapone Potent COMT inhibitor; blocks α-Synuclein aggregation 4720/50 50 mg $169 Constitutive Androstane Receptor (CAR) TCPOBOP CAR agonist 1225/10 10 mg $89 Constitutive Androstane Receptor (CAR) TCPOBOP CAR agonist 1225/50 50 mg $355 CREB-binding Protein (CBP)/p300 Chetomin CBP/p300 inhibitor; blocks CBP/p300 interaction with HIF-1α, HIF-2α, and 4705/1 1 mg $169 STAT2 CXCR4 IT1t dihydrochloride Potent CXCR4 antagonist 4596/10 10 mg $155 CXCR4 IT1t dihydrochloride Potent CXCR4 antagonist 4596/50 50 mg $619 DNA Melphalan DNA alkylating agent; cytotoxic and antineoplastic 4619/50 50 mg $75 Embryonic Stem Cells (ESCs) IQ 1 Prevents spontaneous differentiation of ESCs and enables 4713/10 10 mg $155 Wnt/β-Catenin-driven ESC expansion Embryonic Stem Cells (ESCs) IQ 1 Prevents spontaneous differentiation of ESCs and enables 4713/50 50 mg $619 Wnt/b-Catenin-driven ESC expansion Extracellular Signal-regulated Kinase 2 (ERK2) TCS ERK 11e Potent and selective ERK2 inhibitor 4465/10 10 mg $225 F-actin Jasplakinolide Stabilizes F-actin; promotes actin polymerization 2792/100U 100 µg $275 Furin Hexa-D-arginine Furin inhibitor 4711/1 1 mg $105 GABA Receptors RuBi GABA Caged GABA; inhibits neural activity 4709/10 10 mg $189 trimethylphosphine Glycoprotein IIa/IIIb Eptifibatide Glycoprotein IIa/IIIb inhibitor; inhibits platelet aggregation 4725/1 1 mg $65 G Protein-coupled Receptor 120 (GPR120) TUG 891 Potent and selective GPR120 agonist 4601/10 10 mg $155 G Protein-coupled Receptor 120 (GPR120) TUG 891 Potent and selective GPR120 agonist 4601/50 50 mg $619 Hepatocyte Growth Factor (HCF) Receptor/c-MET Crizotinib Potent HGF R/c-MET inhibitor; also inhibits Anaplastic Lymphoma Kinase 4368/10 10 mg $219 (ALK) Hepatocyte Growth Factor (HCF) Receptor/c-MET Crizotinib Potent HGF R/c-MET inhibitor; also inhibits Anaplastic Lymphoma Kinase 4368/50 50 mg $909 (ALK) HIF-1a KC7F2 HIF-1α inhibitor 4324/10 10 mg $79 HIF-1a KC7F2 HIF-1α inhibitor 4324/50 50 mg $295 Histone Demethylase IOX 1 Cell permeable Histone Demethylase inhibitor 4464/10 10 mg $145 Histone Demethylase IOX 1 Cell permeable Histone Demethylase inhibitor 4464/50 50 mg $645 Continued on page 14.

www.RnDSystems.com 13 Biochemicals & Compounds target Compound name Description Catalog # Size Price Continued from page 13. Histone Methyltransferase 3-Deazaneplanocin A Histone methyltransferase inhibitor 4703/1 1 mg $145 hydrochloride Integrin α1β1 Obtustatin Potent and selective Integrin α1β1 inhibitor 4664/100U 100 µg $249 Janus Kinase (JAK) CP 690550 citrate Potent JAK inhibitor 4556/10 10 mg $145 Janus Kinase (JAK) CP 690550 citrate Potent JAK inhibitor 4556/50 50 mg $645 K+ Channels Cesium chloride K+ channel blocker 4739/50 50 mg $39 Lethal (3) Malignant Brain Tumor-like Protein 3 (L3MBTL3) UNC 1215 Potent and selective cell permeable L3MBTL3 domain inhibitor 4666/10 10 mg $179 Domain Lethal (3) Malignant Brain Tumor-like Protein 3 (L3MBTL3) UNC 1215 Potent and selective cell permeable L3MBTL3 domain inhibitor 4666/50 50 mg $819 Domain Leucine-Rich Repeat Kinase 2 (LRRK2) CZC 54252 hydrochloride Potent LRRK2 inhibitor 4534/10 10 mg $189 Leucine-Rich Repeat Kinase 2 (LRRK2) CZC 54252 hydrochloride Potent LRRK2 inhibitor 4534/50 50 mg $819 Mammalian Target of Rapamycin (mTOR) WYE 687 dihydrochloride Potent and selective mTOR inhibitor 4282/10 10 mg $225 Mammalian Target of Rapamycin (mTOR) WYE 687 dihydrochloride Potent and selective mTOR inhibitor 4282/50 50 mg $945 Metabotropic Glutamate Receptor 5 (mGluR5) VU 0360172 Positive allosteric modulator of mGluR5 4323/10 10 mg $129 hydrochloride Metabotropic Glutamate Receptor 5 (mGluR5) VU 0360172 Positive allosteric modulator of mGluR5 4323/50 50 mg $575 hydrochloride Mitogen-activated Protein Kinase Kinase 1 (MEK1) 10Z-Hymenialdisine Potent MEK1 inhibitor; pan kinase inhibitor 1532/500U 500 µg $299 Nav 1.7 Na+ Channel Huwentoxin IV Selective Nav1.7 Na+ channel blocker 4718/100U 100 µg $189 Na+/K+ ATPase Pump Lithium carbonate Inhibits Na+/K+ ATPase Pump activity 4740/50 50 mg $39 Neuropeptide FF1 Receptor (NPFF1) RFRP 3 (human) NPFF1 agonist; homolog of Gonadotropin-inhibitory hormone 4683/1 1 mg $119

Neuropeptide Y (NPY) Y2 Receptor CYM 9484 Potent NPY Y2 Receptor antagonist 4606/10 10 mg $169

Neuropeptide Y (NPY) Y2 Receptor CYM 9484 Potent NPY Y2 Receptor antagonist 4606/50 50 mg $675 N-metyl D-aspartate (NMDA) Receptor Subtype 2B (NR2B) TCN 237 dihydrochloride Potent and selective NR2B antagonist 4072/10 10 mg $145 Orexin 2 Receptor (OX2) JNJ 10397049 Selective OX2 Receptor antagonist 4317/10 10 mg $179 Orexin 2 Receptor (OX2) JNJ 10397049 Selective OX2 Receptor antagonist 4317/50 50 mg $735

P2X3/P2X2/3 Purinergic Receptor TC-P 262 Selective P2X3/P2X2/3 Receptor antagonist 4386/10 10 mg $129

P2X3/P2X2/3 Purinergic Receptor TC-P 262 Selective P2X3/P2X2/3 Receptor antagonist 4386/50 50 mg $575 Peroxisome Proliferator-activated Receptor (PPAR) γ SR 1664 High affinity PPARγ ligand; blocks Cyclin-dependent Kinase 5 4409/10 10 mg $169 (Cdk5)-dependent PPARγ phosphorylation Peroxisome Proliferator-activated Receptor (PPAR) γ SR 1664 High affinity PPARγ ligand; blocks Cyclin-dependent Kinase 5 4409/50 50 mg $735 (Cdk5)-dependent PPARγ phosphorylation P-glycoprotein (P-gp) Elacridar hydrochloride P-gp inhibitor 4646/10 10 mg $189 Plasma Membrane Monoamine Transporter (PMAT) Decynium 22 PMAT inhibitor 4722/50 50 mg $49 Protein Kinase C (PKC) PEP 005 PKC activator 4054/1 1 mg $179 Protein Kinase C (PKC) Bisindolylmaleimide II Potent PKC inhibitor; antagonist of Nicotinic Acetylcholine Receptors (nAChR) 4128/10 10 mg $169 Protein Kinase C (PKC) Bisindolylmaleimide II Potent PKC inhibitor; antagonist of Nicotinic Acetylcholine Receptors (nAChR) 4128/50 50 mg $795 Protein Kinase C (PKC)/NFκB CID 2858522 Selectively inhibits PKC-induced NF-κB activation 4246/10 10 mg $125 Protein Kinase D (PKD) CID 2011756 Cell permeable pan PKD inhibitor 4644/10 10 mg $189 Protein Kinase D (PKD) CID 2011756 Cell permeable pan PKD inhibitor 4644/50 50 mg $819 REV-Erbα SR 8278 REV-Erbα antagonist 4463/10 10 mg $145 REV-Erbα SR 8278 REV-Erbα antagonist 4463/50 50 mg $645 RNA 8-Chloroadenosine Cytotoxic nucleoside analog; inhibits RNA synthesis 4436/10 10 mg $169 RNA 8-Chloroadenosine Cytotoxic nucleoside analog; inhibits RNA synthesis 4436/50 50 mg $795

14 For research use only. Not for use in diagnostic procedures. target Compound name Description Catalog # Size Price Serotonin 5-HT2C Receptor S 32212 hydrochloride 5-HT2C Receptor inverse agonist; α2-adrenoceptor antagonist 4508/10 10 mg $179 Serotonin 5-HT2C Receptor S 32212 hydrochloride 5-HT2C Receptor inverse agonist; α2-adrenoceptor antagonist 4508/50 50 mg $735 Serotonin 5-HT2/D2 Receptor Quetiapine 5-HT2/D2 Receptor antagonist; atypical antipsychotic 4735/50 50 mg $99 hemifumarate Serotonin 5-HT7 Receptor LP 20 hydrochloride Selective 5-HT7 Receptor ligand 4704/10 10 mg $155 Serotonin 5-HT7 Receptor LP 20 hydrochloride Selective 5-HT7 Receptor ligand 4704/50 50 mg $619 Smoothened (Smo) SMANT hydrochloride Inhibits Smo accumulation 4662/10 10 mg $125 Smoothened (Smo) SMANT hydrochloride Inhibits Smo accumulation 4662/50 50 mg $495 Sphingosine Kinase (SphK) N,N- SphK inhibitor 4640/10 10 mg $119 Dimethylsphingosine Sphingosine 1-Phosphate Receptor-1 (S1P1) W146 Potent and selective S1P1 receptor antagonist 3602/1 1 mg $135 TCF/β-Catenin CCT 031374 Inhibitor of TCF/β-Catenin-mediated transcription 4675/10 10 mg $145 hydrobromide TCF/β-Catenin CCT 031374 Inhibitor of TCF/β-Catenin-mediated transcription 4675/50 50 mg $599 hydrobromide Threonyl-tRNA Synthetase Borrelidin Threonyl-tRNA Synthetase inhibitor; anti-angiogenic 4706/1 1 mg $345 T-LAK-cell-originated Protein Kinase (TOPK) HI TOPK 032 Selective TOPK inhibitor 4555/10 10 mg $199 Toll-like Receptor 1/2 (TLR1/2) Pam3CSK4 TLR1/2 agonist; induces cytokine production 4633/1 1 mg $145 Toll-like Receptor 2/6 (TLR2/6) Pam2CSK4 TLR2/6 agonist; induces TNF-α production 4637/1 1 mg $155 Transglutaminase 2 (TG2) LDN 27219 Reversible TG2 inhibitor 4602/10 10 mg $159 Transglutaminase 2 (TG2) LDN 27219 Reversible TG2 inhibitor 4602/50 50 mg $679 Transient Receptor Potential Channel, subfamily A, A 967079 Selective TRPA1 channel blocker 4716/10 10 mg $125 member 1 (TRPA1) Transient Receptor Potential Channel, subfamily A, A 967079 Selective TRPA1 channel blocker 4716/50 50 mg $495 member 1 (TRPA1) Transient Receptor Potential Channel 4 (TRPC4) ML 204 TRPC4 blocker 4732/10 10 mg $125 Transient Receptor Potential Channel 4 (TRPC4) ML 204 TRPC4 blocker 4732/50 50 mg $495 Transient Receptor Potential Channel, subfamily V, α-Spinasterol TRPV1 antagonist; displays in vivo activity 4729/1 1 mg $95 member 1 (TRPV1) Ubiquitin Specific Peptidase 7 (USP7) P005091 USP7 inhibitor 4733/10 10 mg $145 Ubiquitin Specific Peptidase 7 (USP7) P005091 USP7 inhibitor 4733/50 50 mg $645

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MuRF1/S5a Ubiquitination Kit Boston Biochem is now offering a MuRF1/S5a Ubiquitination Kit that is designed for the in vitro Reaction Time (minutes) ubiquitination of user-supplied substrates by MuRF1 (Catalog # K-102). MuRF1 is an E3 ligase that is 0 10 20 30 45 60 90 kDa expressed in cardiac and skeletal muscle and is thought to mediate muscle atrophy.1,2 Accordingly, it 150 has been reported to inhibit cardiac hypertrophy, and mutations in MuRF1 have been identified in patients with hypertrophic cardiomyopathy.3,4 The MuRF1/S5a Ubiquitination Kit is ideal for use as a positive control for ubiquitination of known MuRF1 substrates or for the discovery of novel substrates. 75 S5a-Ub It can also be used for mutational analysis studies to determine which domains or specific amino acids n S5a within the substrate are important for ubiquitination by MuRF1. Additionally, the kit provides a 50 controlled environment to study pharmacological compounds that alter MuRF1 activity.5 For more information on ubiquitination, please visit the Boston Biochem website at www.BostonBiochem.com. In Vitro Ubiquitination of a Protein Substrate by MuRF1. An in vitro ubiquitination reaction containing the MuRF1, His6-S5a control substrate, E1 and E2 enzymes, Ubiquitin, reaction buffer, and Mg2+-ATP provided in the Human MuRF1/S5a Ubiquitination Kit (Catalog # K-102) was conducted for the New Products from Boston Biochem indicated times. Ubiquitinated His6-S5a was detected by Western blot using protein or Molecule species source catalog# size price the Goat Anti-Mouse S5a/Angiocidin Antigen Affinity-purified Polyclonal Antibody included in the kit. The ladder of slower migrating bands represents MuRF1/TRIM63 Ubiquitination Kit Human NA K-102 10 x 30 µL $350 poly-ubiquitinated His6-S5a. Reactions Di-Ubiquitin/Ub2 Non-hydrolyzable (K11-linked) Human E. coli UCN-40-025 25 µg $150 References Ubiquitin Chain 1. Kedar, V. et al. (2004) Proc. Natl. Acad. Sci. USA CHIP/STUB1 Ubiquitiin Ligase (E3) Human E. coli E3-220-050 50 µg $250 101:18135. E6AP/UBE3A Ubiquitiin Ligase (E3) Human Sf 21 E3-230-050 50 µg $250 2. Lokireddy, S. et al. (2011) Am. J. Physiol. Cell Physiol. 301:C1316. HPV E6 Ubiquitiin Ligase (E3) Viral E. coli AP-120-025 25 µg $100 3. Li, H.H. et al. (2011) Am. J. Pathol. 178:1043. FAM105B/OTULIN Deconjugating Enzyme Human E. coli E-558-050 50 µg $150 4. Chen, S.N. et al. (2012) Circ. Res. 111:907. 5. Eddins, M.J. et al. (2011) Cell Biochem. Biophys. 60:113. 10X E3 Ligase Reaction Buffer NA NA B-71 5 mL $30

1616 www.RnDSystems.com