RESEARCH USE ONLY

POLYCLONAL ANTIBODY Anti-BAFF (B-cell Activating Factor)

Code No. Host Quantity JM-3203-100 Rabbit IgG 100 µg

BACKGROUND: Members in the TNF superfamily regulate immune responses and induce . A novel member in the TNF family was recently identified by several groups and designated BAFF (for B cell Activating Factor belonging to the TNF Family), BLyS (for B Lymphocyte Stimulator), TALL-1 (for TNF- and ApoL–related Leukocyte-expressed ), and THANK (for TNF Homologue that activates apoptosis, NFκB, and c-jun N-terminal Kinase). BAFF/BLyS was characterized as a B-cell stimulator since it induced B-cell proliferation and immunoglobulin secretion. Two receptors for BAFF were recently identified and designated TACI and BCMA. BAFF and its receptors are involved in the development of systemic lupus erythaematosus and other B-cell associated autoimmune diseases. Like TNF-α and , THANK was shown to activate NF-κB and c-jun N-terminal kinase (JNK) and to induce apoptosis.

PRODUCT: Supplied as 100 μg (0.5 mg/ml) affinity purified, rabbit anti-BAFF polyclonal antibody in phosphate buffered saline (PBS), pH 7.2, containing 50% glycerol, 1% BSA, 0.02% sodium azide.

SPECIFICITY: The immunoaffinity purified antibody detects human, mouse and rat BAFF. HL60 cell lysate can be used as a positive control.

APPLICATIONS: The antibody can be used for Western blot analysis (1 μg/ml). However, the optimal conditions should be determined individually.

STORAGE: Store the antibody at -20°C. For long-term storage, aliquot and freeze at -70°C. Avoid freeze/thaw cycles.

INTENDED USE: For research use only. Not for clinical diagnosis or therapeutic use.

REFERENCES: 1. Moore, P.A., et al (1999) Science 285:260-263. 2. Schneider, P., et al. (1999) J. Exp. Med. 189:1747-1756.

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