For Research Use Only

SRD5A1 Monoclonal antibody Catalog Number:66329-1-Ig 3 Publications www.ptglab.com

Catalog Number: GenBank Accession Number: Purification Method: Basic Information 66329-1-Ig BC007033 Protein G purification Size: GeneID (NCBI): CloneNo.: 150UL , Concentration: 1000 μg/ml by 6715 1D5D8 Bradford method using BSA as the Full Name: Recommended Dilutions: standard; -5-alpha-reductase, alpha WB 1:500-1:2000 Source: polypeptide 1 (3-oxo-5 alpha-steroid IHC 1:50-1:500 Mouse delta 4-dehydrogenase alpha 1) IF 1:50-1:500 Isotype: Observed MW: IgG1 32 kDa Immunogen Catalog Number: AG23351

Applications Tested Applications: Positive Controls: IF, IHC, WB, ELISA WB : human testis tissue, Cited Applications: IHC : human tissue, IHC, WB IF : HeLa cells, Species Specificity: human Cited Species: human Note-IHC: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Steroid 5-alpha-reductase (SRD5A1) is a minor component of the reductase activity in prostate although the Background Information was originally cloned from prostate. On the other hand, SRD5A1 appears to be the predominant of steroid 5- alpha-reductase in the scalp and elsewhere in the skin.

Notable Publications Author Pubmed ID Journal Application Renjie Dou 33627630 Cell Death Dis WB Mitsuki Nozaki 29391479 Sci Rep WB Rongfang Wei 32983992 Front Oncol WB,IHC

Storage: Storage Store at -20°C. Stable for one year after shipment. Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Aliquoting is unnecessary for -20ºC storage

For technical support and original validation data for this product please contact: This product is exclusively available under Proteintech T: 1 (888) 4PTGLAB (1-888-478-4522) (toll free E: [email protected] Group brand and is not available to purchase from any in USA), or 1(312) 455-8498 (outside USA) W: ptglab.com other manufacturer. Selected Validation Data

human testis tissue were subjected to SDS PAGE Immunohistochemical analysis of paraffin- Immunohistochemical analysis of paraffin- followed by western blot with 66329-1-Ig (SRD5A1 embedded human liver tissue slide using 66329-1- embedded human liver tissue slide using 66329-1- Antibody) at dilution of 1:1000 incubated at room Ig (SRD5A1 Antibody) at dilution of 1:200 (under Ig (SRD5A1 Antibody) at dilution of 1:200 (under temperature for 1.5 hours. 10x lens). 40x lens).

Immunofluorescent analysis of (-20 Ethanol ) fixed HeLa cells using 66329-1-Ig(SRD5A1 antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L).