Table 2. Partial List of Genes Differentially Expressed

Table 2. Partial list of genes differentially expressed during osteoblast differentiation (P < 0.05, ratio >2or<0.5)

P value P value P value D1B͞ D1B vs. D2B͞ D2B vs. D7B͞ D7B vs. Acc. no. Gene Title MPC MPC MPC MPC MPC MPC

Apoptosis and cell cycle related AA455281 DAD1 Defender against cell death 1 0.70 0.32 0.46 0.15 0.34 0.04 R43325 DAP3 Death associated protein 3 0.41 0.15 0.28 0.04 0.41 0.10 R43325 DAP3 Death associated protein 3 0.53 0.05 0.40 0.02 0.50 0.12 H44953 CASP4 Caspase 4, apoptosis-related cysteine protease 0.62 0.23 0.46 0.02 0.56 0.13 N70463 BTG1 B cell translocation gene 1, anti-proliferative 2.12 0.01 2.64 0.07 3.49 0.25 H69583 BTG2 BTG family, member 2 1.68 0.19 2.88 0.10 2.93 0.05 AA633993 CDC10 Cell division cycle 10 (homologous to CDC10 of S. cerevisiae) 0.45 0.08 0.31 0.01 0.54 0.14 AA598974 CDC2 Cell division cycle 2, G1 to S and G2 to M 0.31 0.04 0.40 0.12 0.38 0.09 N35067 CDC2L5 Cell division cycle 2-like 5 1.36 0.51 1.73 0.36 3.04 0.01 AA789328 CDK10 Cyclin-dependent kinase (CDC2-like) 10 1.29 0.37 1.86 0.16 2.13 0.02 H10959 DBCCR1 Deleted in bladder cancer chromosome region candidate 1 1.81 0.02 1.85 0.09 3.19 0.02 AA679864 DLC1 Deleted in liver cancer 1 0.43 0.06 0.33 0.01 0.37 0.03 AA461110 GAS6 Growth arrest-speciﬁc 6 1.90 0.35 4.15 0.25 3.24 0.05 N33574 ING1 Inhibitor of growth family, member 1 1.39 0.26 1.92 0.12 2.19 0.00 AA481076 MAD2L1 MAD2 (mitotic arrest deﬁcient, yeast, homolog)-like 1 0.38 0.05 0.39 0.04 0.68 0.33 DNA͞RNA AA485944 DDX17 DEAD͞H (Asp-Glu-Ala-Asp͞His) box polypeptide 17 (72kD) 0.88 0.28 1.38 0.52 2.14 0.05 AA127093 HDAC2 Histone deacetylase 2 0.69 0.08 0.53 0.09 0.41 0.02 AA683085 HMG1 High-mobility group (nonhistone chromosomal) protein 1 0.32 0.03 0.28 0.05 0.57 0.22 N72057 HMG17 High-mobility group (nonhistone chromosomal) protein 17 2.35 0.28 2.34 0.18 5.76 0.03 AA448261 HMGIY High-mobility group (nonhistone chromosomal) protein isoforms I and Y 0.62 0.11 0.43 0.02 0.36 0.03 AA487148 TAF2C1 TATA box binding protein-associated factor, RNA polymerase II, C1, 0.52 0.19 0.80 0.49 0.28 0.00 AA857343 TAF2N TATA box binding protein-associated factor, RNA polymerase II, N, 8.08 0.03 7.67 0.02 10.35 0.01 AA453787 TAF3C TATA box binding protein-associated factor, RNA polymerase III, C, 2.26 0.25 3.71 0.19 4.74 0.03 Matrix

N94385 COMP Cartilage oligomeric matrix protein 1.32 0.34 1.47 0.60 2.46 0.02 BIOLOGY AA504891 CRYAB Crystallin, alpha B 5.23 0.04 4.12 0.08 3.30 0.06 AA134871 FBLN1 Fibulin 1 1.79 0.04 4.74 0.09 19.26 0.00 EVELOPMENTAL AA485748 FMOD Fibromodulin 1.30 0.40 1.48 0.03 3.05 0.02 AA180013 HABP2 Hyaluronan-binding protein 2 1.84 0.18 2.36 0.23 3.01 0.01 R42600 MMP17 Matrix metalloproteinase 17 (membrane-inserted) 2.83 0.24 4.76 0.18 6.84 0.05 AA669222 MMP19 Matrix metalloproteinase 19 1.43 0.43 1.67 0.08 2.48 0.04 AA936799 MMP2 Matrix metalloproteinase 2 2.02 0.17 2.12 0.03 5.49 0.06 N32201 OMD Osteomodulin 0.98 0.76 1.57 0.15 1.97 0.03 Receptor and ligand AA779480 BMP8 Bone morphogenetic protein 8 (osteogenic protein 2) 13.76 0.22 14.50 0.36 24.84 0.11 AA443899 CD36L1 CD36 antigen (collagen type I receptor, thrombospondin receptor)-like 1 2.18 0.18 2.98 0.16 3.48 0.03 AA136983 CDH11 Cadherin 11 (OB-cadherin, osteoblast) 1.90 0.04 1.52 0.16 1.95 0.05 AA454657 IL11RA IL-11 receptor, alpha 1.73 0.35 2.29 0.05 2.92 0.02 AA126200 IL1RL1 IL-1 receptor-like 1 1.24 0.25 2.08 0.24 2.71 0.03 AA872602 PTHR1 Parathyroid hormone receptor 1 3.97 0.28 5.90 0.25 7.82 0.05 AA464532 THBS1 Thrombospondin 1 1.24 0.49 1.78 0.10 2.32 0.01 AA194983 TNFRSF11B TNF receptor superfamily, member 11b (osteoprotegerin) 1.22 0.60 1.71 0.00 3.07 0.04 W71984 TNFRSF12 TNF receptor superfamily, member 12 1.63 0.39 2.01 0.33 2.76 0.04 AA147594 TNFRSF8 TNF receptor superfamily, member 8 1.55 0.29 1.88 0.12 2.45 0.02 N35070 TNFSF12 TNF (ligand) superfamily, member 12 1.78 0.21 2.19 0.09 3.54 0.00 Signal transduction AA454702 CRABP1 Cellular retinoic acid-binding protein 1 1.78 0.51 3.40 0.51 4.29 0.03 H51404 LIMK1 LIM domain kinase 1 1.83 0.21 2.24 0.17 4.68 0.01 H39192 MAPK7 Mitogen-activated protein kinase 7 1.44 0.06 1.60 0.23 2.89 0.02 H22922 MFNG Manic fringe (Drosophila) homolog 1.39 0.08 2.36 0.12 3.54 0.05 AA480906 PRKCBP1 Protein kinase C binding protein 1 0.63 0.04 0.44 0.04 0.55 0.08 R61007 PTP4A1 Protein tyrosine phosphatase type IVA, member 1 0.64 0.16 0.36 0.05 0.44 0.09 AA482067 RARRES2 Retinoic acid receptor responder (tazarotene induced) 2 1.95 0.26 1.86 0.01 2.91 0.00 AA485355 SSI-1 JAK binding protein 1.43 0.66 2.38 0.09 2.91 0.00 AA001219 SSI-3 STAT-induced STAT inhibitor 3 0.44 0.05 0.55 0.11 0.74 0.36 AA137031 STATI2 STAT-induced STAT inhibitor-2 1.83 0.44 1.90 0.28 2.76 0.01 TF AA775091 DSIPI Delta sleep inducing peptide, immunoreactor 6.15 0.05 6.27 0.04 7.32 0.09 AA424950 E2F1 E2F transcription factor 1 1.27 0.50 1.53 0.65 2.95 0.04 W86216 ERF Ets2 repressor factor 1.17 0.48 1.35 0.29 2.01 0.01 R06446 GATA1 GATA-binding protein 1 (globin transcription factor 1) 1.86 0.01 2.63 0.01 3.40 0.00 AA479196 GTF2F2 General transcription factor IIF, polypeptide 2 (30kD subunit) 0.53 0.01 0.39 0.01 0.48 0.04 AA460838 GTF2H3 General transcription factor IIH, polypeptide 3 (34kD subunit) 1.14 0.95 2.36 0.04 1.70 0.16 AA610066 HOXB6 Homeo box B6 1.77 0.01 1.84 0.20 2.11 0.02 AA482119 ID3 Inhibitor of DNA binding 3, dominant negative helix-loop-helix protein 0.44 0.00 0.49 0.06 0.41 0.01 H65734 KLF1 KLF 1 (erythroid) 1.91 0.17 1.96 0.04 2.58 0.05 H73914 LDB2 LIM binding domain 2 2.77 0.04 1.71 0.00 1.31 0.61 AA447515 MAD4 Mad4 homolog 1.38 0.52 1.72 0.49 3.08 0.00 AA464600 MYC v-myc avian myelocytomatosis viral oncogene homolog 0.27 0.01 0.20 0.01 0.27 0.03 H38522 POU2F1 POU domain, class 2, transcription factor 1 1.43 0.14 2.05 0.07 3.16 0.05 AA479928 SHOX2 Short stature homeobox 2 1.76 0.29 2.81 0.14 3.01 0.01 AA428551 SOX22 SRY (sex-determining region Y)-box 22 1.68 0.09 2.16 0.18 2.39 0.01 AA453420 SOX4 SRY (sex determining region Y)-box 4 1.78 0.43 1.88 0.38 2.35 0.02 AA488075 STAT1 Signal transducer and activator of transcription 1, 91kD 0.42 0.03 0.45 0.09 0.55 0.15 H17048 ZNF133 Zinc ﬁnger protein 133 (clone pHZ-13) 1.44 0.52 1.80 0.06 3.07 0.00 AA669341 ZNF6 Zinc ﬁnger protein 6 (CMPX1) 0.43 0.05 0.28 0.05 0.30 0.10