Safety Data Sheet Proteins in Storage Buffers Part #: SDS0007 Effective Date: 12/2012

Section 1: Chemical and Company Identification EPICENTRE TECHNOLOGIES® an Illumina® Company 5602 Research Park Blvd., MADISON WI 53719 Emergency Tel: 608-258-3080 or 800-284-8474 7:30-4:30 Central

Chemical Name: The products below are proteins isolated from non-hazardous . These proteins present no known threat to humans or laboratory animals. The storage solution is supplied in a 50/50 glycerol:H2O solution containing salts and a detergent. The salts and detergent are present at less than 1% concentrations and represent no known hazards. A (material) safety data sheet for glycerol is provided.

This document serves as a cover page and part of section 1 of the safety data sheets for the following EPICENTRE names and catalog numbers:

Ampligase® Themalstable DNA A1150K Apex™ Heat-Label Alkaline AP49050, AP49100, AP49010 RecBCD , E. coli BCD0401K rBST DNA Polymerase BH101K, BH1100 rBST DNA Polymerase Large Fragment (IsoTherm™ DNA Polymerase) BL1950K, BL901K, E. coli RNA Polymerase Core C90100, C90500 T4 DNA Polymerase D0602H, D0605H T7 R&DNA™ Polymerase D7P9201K, D7P9205K, D7P9205K, Baseline-ZERO™ DNase DB0711K, DB715K DisplaceAce™ DNA Polymerase D090710K, E. coli DNA Ligase D L04082H, DNA Polymerase I, E. coli DP0810500 PlasmidSafe™ ATP-Dependent DNase E3101K, E3105K, E3110K IV, E. coli E70100, VII EN510100, EN510250 Exonuclease II, E.coli EX4405K, EX4425K GELase™ Agarose Gel-Digesting Preparation G09050, G09100, G09200, G31050 G31200, T4 Beta-glucosyltransferase GT11500, GT112500 Hybridase™ Thermostable RNase H H39100, H39500 HK™ Phosphatase H92100 HK™-UNG Thermolabile Uracil N-Glycosylase HU59100, HU5901K, Exo-Minus Klenow DNA Polymerase KL0705K, KL081001K, KL080911K, KL0810250, KL105000, KL111K, KL115K, KL11250, KL11101K Klenow DNA Polymerase (Klenow Large Fragment) KP8100K, KP810250 T4 DNA Ligase, Cloned L0805H, L0810H, L0820H, LH805H, LH810H, LH820H Lambda Exonuclease LE035H, LE032K, T4 RNA Ligase LR5010, LR5025, T4 RNA Ligase 2, deletion mutant LR2D11310K, LR2D1132K Lambda Terminase LT4450, LT44200, MonsterScript™ Reverse Transcriptase MSTA5110, MSTA5124 RNase I, E. coli N6901K, N6905K, RNAse T1, Aspergillus orzyae NT09100K, NT09500K NTPhos™ Thermolabile Phosphatase NT4910K, NT4920K OmniCleave™ Endonuclease OC7810K, OC7850K, 8-oxoguanine-DNA Excision Mix OG51100, T4 Polynucleotide Kinase P0501K, P0503K, P0505H MasterAmp™ Extra-Long DNA Polymerase Mix QU92125, QU92500, QU9201K Ready-Lyse™ Lysozyme Solution R1802M, R1804M, R1810M RNase H, E. coli R52250, R0601K Rec A Protein, E. coli RC44200, RC441MG RiboGuard™ RNase Inhibitor RG90910K, RG90925, RecJ Exonuclease RJ411050, RJ411250, RNase III, E. coli RN02950, R RNR07250 RiboShredder™ RNase Blend RS12100, RS12500, MMLV High Performance Reverse Transcriptase RT80110K, RT80125K, Pvu Tts1l Endonuclease RTS11100 SP6 RNA Polymerase S4905K, S4950K, SM910K E. coli RNA Polymerase Sigma Saturated Holoenzyme S90050, S90250 Single-Strand Binding Protein (SSB) SSB02200 T5 Exonuclease T5E4111K T7 RNA Polymerase T7905K, T7950K, TH950K, TM910K T3 RNA Polymerase T4905K, T9050K Terminal deoxynucleotidyl , Recombinant TDT11725K, TDT117500, T4 Endonuclease V TE6605, TE661K, TE665K Page 1 of 2 Safety Data Sheet Proteins in Storage Buffers Part #: SDS0007 Effective Date: 12/2012

Terminator™ 5’-Phsophate-Dependent TER51020 TypeOne™ Restriction Inhibitor TY0261H Uracil-DNA Excision Mix UEM04100, DNA Topoisomerase I, Vaccinia VT710500, VT101K, VT7105K Exonuclease I, E. coli X40501K, X40505K, X40520K.

Tris acetate,10X is supplied as a reaction buffer for several of the including 10X DNase Buffer, 10X T4 Polynucleotide Kinase Buffer, TdT 10X Reaction Buffer (TDT11725K and TDT117500), T4 Beta-glucosyltransferase 10X Reaction Buffer (GT11500), Pvu Rts1l 10X Reaction Buffer (RTS11100),10X Klenow Fill-in Buffer and 10X FastLink™ Buffer, represented by catalog numbers: AP49010, AP49050, AP49100, AP89180, BCD0401K, D0602H, D0605H, E3101K, E3105K, E3110K, EX44050K, EX44200, H92100, L0805H, L0810H, L0820H, LH805H, LH810H, LH820H, LR5010, LR5025, LT4450, LT44200, P0505H, P0503K, P0501K, P0510K, RJ411050, RJ411250, T5E4111K. An MSDS/SDS is provided for Tris acetate.

50X GELase™ buffer supplied with products G09050, G09100, G09200, G31050 G31200 contains BisTris. A safety data sheet is supplied for BisTris.

TDT11725K and TDT117500 representing Terminal deoxynucleotidyl Transfersase, Recombinant contains 2.5 mM cobalt chloride as a component. A safety data sheet is provided for cobalt chloride.

T4 Beta-glucosyltransferase is supplied with 10 mM UDP-Glucose. An SDS is provided for UDP-Glucose.

Tris is an ingredient of the 10 and 5X Reaction Buffers for BL901K, D7P9201K, DB0711K, D090710K, DB715K, D69305K, D7P9205K, DL04082H, G0808250, KL115K, KL11101K, KL11250, M4410K, M4425H, N6901K, N6905K, OG51100, RNR07250, LR2D11310K, LR2D1132K, S4905K, S4950K, SM910K, T4905K, T7905K, T7950K, T9050K, TER51020, TM910K, TH950K, & UEM04100. A safety data sheet is provided for Tris.

10X Reaction Buffers for Exo-Minus Klenow DNA Polymerase represented by part numbers KL115K, KL11101K and KL11250 contain DTT in addition to Tris. An SDS is available for DTT.

EPICENTRE recommends all normal precautions be taken whenever all hazards are not known. Wear gloves and avoid direct contact with skin and eyes when handling chemical solutions.

This information is believed to be correct; however, EPICENTRE® Biotechnologies makes no guarantee of the accuracy or completeness of the data and shall not be liable for any damages. This document is offered solely as a guide for the customer's consideration, investigation and verification. These suggestions do not replace state, municipal and/or insurance requirements. Any use of this information must be determined by the user to be in accordance with applicable Federal, state and local regulations.

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