Anti-CEMIP Antibody (ARG59980)

Product datasheet [email protected]

ARG59980 Package: 100 μl anti-CEMIP antibody Store at: -20°C

Summary

Product Description Rabbit Polyclonal antibody recognizes CEMIP

Tested Reactivity Hu, Ms

Tested Application WB

Host Rabbit

Clonality Polyclonal

Isotype IgG

Target Name CEMIP

Antigen Species Human

Immunogen Recombinant fusion protein corresponding to aa. 180-420 of Human CEMIP (NP_061159.1).

Conjugation Un-conjugated

Alternate Names CCSP1; Cell migration-inducing and hyaluronan-binding protein; EC 3.2.1.35; KIAA1199; TMEM2L

Application Instructions

Application table Application Dilution

WB 1:200 - 1:2000

Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Positive Control Mouse brain and U87

Calculated Mw 153 kDa

Observed Size 180 kDa

Properties

Form Liquid

Purification Affinity purified.

Buffer PBS (pH 7.3), 0.02% Sodium azide and 50% Glycerol.

Preservative 0.02% Sodium azide

Stabilizer 50% Glycerol

Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.

Note For laboratory research only, not for drug, diagnostic or other use.

www.arigobio.com 1/2 Bioinformation

Gene Symbol CEMIP

Gene Full Name cell migration inducing protein, hyaluronan binding

Function Mediates depolymerization of hyaluronic acid (HA) via the cell membrane-associated clathrin-coated pit endocytic pathway. Binds to hyaluronic acid. Hydrolyzes high molecular weight hyaluronic acid to produce an intermediate-sized product, a process that may occur through rapid vesicle endocytosis and recycling without intracytoplasmic accumulation or digestion in lysosomes. Involved in hyaluronan catabolism in the dermis of the skin and arthritic synovium. Positively regulates epithelial-mesenchymal transition (EMT), and hence tumor cell growth, invasion and cancer dissemination. In collaboration with HSPA5/BIP, promotes cancer cell migration in a calcium and PKC-dependent manner. May be involved in hearing. [UniProt]

Cellular Localization Nucleus. Cytoplasm. Endoplasmic reticulum. Cell membrane. Membrane, clathrin-coated pit. Secreted. Note=Retained in the endoplasmic reticulum (ER) in a HSPA5/BIP-dependent manner. Colocalized with clathrin heavy chain/CLTC in clathrin-coated vesicles. Strongly detected in the cytoplasm of breast carcinoma cells, whereas poorly detected in adjacent normal epithelial cells, stromal cells, or benign breast tissues. Localized in the nucleus and cytoplasm of colon adenocarcinomas. [UniProt]

Images

ARG59980 anti-CEMIP antibody WB image

Western blot: 25 µg of Mouse brain and U87 cell lysates stained with ARG59980 anti-CEMIP antibody at 1:3000 dilution.