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How Many Fungi Make Sclerotia?
fungal ecology xxx (2014) 1e10 available at www.sciencedirect.com ScienceDirect journal homepage: www.elsevier.com/locate/funeco Short Communication How many fungi make sclerotia? Matthew E. SMITHa,*, Terry W. HENKELb, Jeffrey A. ROLLINSa aUniversity of Florida, Department of Plant Pathology, Gainesville, FL 32611-0680, USA bHumboldt State University of Florida, Department of Biological Sciences, Arcata, CA 95521, USA article info abstract Article history: Most fungi produce some type of durable microscopic structure such as a spore that is Received 25 April 2014 important for dispersal and/or survival under adverse conditions, but many species also Revision received 23 July 2014 produce dense aggregations of tissue called sclerotia. These structures help fungi to survive Accepted 28 July 2014 challenging conditions such as freezing, desiccation, microbial attack, or the absence of a Available online - host. During studies of hypogeous fungi we encountered morphologically distinct sclerotia Corresponding editor: in nature that were not linked with a known fungus. These observations suggested that Dr. Jean Lodge many unrelated fungi with diverse trophic modes may form sclerotia, but that these structures have been overlooked. To identify the phylogenetic affiliations and trophic Keywords: modes of sclerotium-forming fungi, we conducted a literature review and sequenced DNA Chemical defense from fresh sclerotium collections. We found that sclerotium-forming fungi are ecologically Ectomycorrhizal diverse and phylogenetically dispersed among 85 genera in 20 orders of Dikarya, suggesting Plant pathogens that the ability to form sclerotia probably evolved 14 different times in fungi. Saprotrophic ª 2014 Elsevier Ltd and The British Mycological Society. All rights reserved. Sclerotium Fungi are among the most diverse lineages of eukaryotes with features such as a hyphal thallus, non-flagellated cells, and an estimated 5.1 million species (Blackwell, 2011). -
Links Between Genetic Groups, Indole Alkaloid Profiles and Ecology Within the Grass-Parasitic Claviceps Purpurea Species Complex
Toxins 2015, 7, 1431-1456; doi:10.3390/toxins7051431 OPEN ACCESS toxins ISSN 2072-6651 www.mdpi.com/journal/toxins Article Links between Genetic Groups, Indole Alkaloid Profiles and Ecology within the Grass-Parasitic Claviceps purpurea Species Complex Mariell Negård 1,2, Silvio Uhlig 1,3, Håvard Kauserud 2, Tom Andersen 2, Klaus Høiland 2 and Trude Vrålstad 1,2,* 1 Norwegian Veterinary Institute, P.O. Box 750 Sentrum, 0106 Oslo, Norway; E-Mails: [email protected] (M.N.); [email protected] (S.U.) 2 Department of Biosciences, University of Oslo, P.O. Box 1066 Blindern, 0316 Oslo, Norway; E-Mails: [email protected] (H.K.); [email protected] (T.A.); [email protected] (K.H.) 3 Department of the Chemical and Biological Working Environment, National Institute of Occupational Health, P.O. Box 8149 Dep, 0033 Oslo, Norway * Author to whom correspondence should be addressed; E-Mail: [email protected]; Tel.: +47-2321-6247. Academic Editor: Christopher L. Schardl Received: 3 January 2015 / Accepted: 22 April 2015 / Published: 28 April 2015 Abstract: The grass parasitic fungus Claviceps purpurea sensu lato produces sclerotia with toxic indole alkaloids. It constitutes several genetic groups with divergent habitat preferences that recently were delimited into separate proposed species. We aimed to 1) analyze genetic variation of C. purpurea sensu lato in Norway, 2) characterize the associated indole alkaloid profiles, and 3) explore relationships between genetics, alkaloid chemistry and ecology. Approximately 600 sclerotia from 14 different grass species were subjected to various analyses including DNA sequencing and HPLC-MS. -
The Response of Dark Septate Endophytes (DSE) to Heavy Metals in Pure Culture
The Response of Dark Septate Endophytes (DSE) to Heavy Metals in Pure Culture Yihui Ban1, Ming Tang2*, Hui Chen2, Zhouying Xu1, Haihan Zhang3, Yurong Yang2 1 College of Life Sciences, Northwest A & F University, Yangling, Shaanxi, China, 2 College of Forestry, Northwest A & F University, Yangling, Shaanxi, China, 3 School of Environmental and Municipal Engineering, Xi’an University of Architecture and Technology, Xi’an, Shannxi, China Abstract Dark septate endophytes (DSE) occur widely in association with plants exposed to heavy metal stress. However, little is known about the response of DSE exposed to heavy metals. In this study, five DSE were isolated from the roots of Astragalus adsurgens Pall. seedlings growing on lead-zinc mine tailings in China. Based on morphological characteristics and DNA sequence analyses, the isolates were identified as Gaeumannomyces cylindrosporus, Paraphoma chrysanthemicola, Phialophora mustea, Exophiala salmonis, and Cladosporium cladosporioides. G. cylindrosporus was selected to explore responses to Pb stress. Scanning electron microscopic observations of G. cylindrosporus grown on solid medium revealed curling of hyphae and formation of hyphal coils in response to Pb. In contrast, in liquid medium, hyphae became thick and swollen with an increase in Pb (II) concentration. We interpret that these changes are related to the variation in cell wall components. We also demonstrated that fungal melanin content increased with the addition of Pb(II). Melanin, as an important component in the cell wall, is known to be an essential antioxidant responsible for decreasing heavy metal toxicity. We also measured the total soluble protein content and glutathione (GSH) concentrations in G. cylindrosporus and found that they initially increased and then decreased with the increase of Pb(II) concentrations. -
Claviceps.Identifica
A Laboratory Guide to the Identification of Claviceps purpurea and Claviceps africana in Grass and Sorghum Seed Samples sponsored by: The Mexican Seed Trade Association The American Seed Trade Association The Oregon Seed Trade Association Texas Seed Trade Association April 19, 1999 authors: Stephen Alderman, Ph.D., Plant Pathologist, USDA-ARS, National Forage Seed Produc- tion Research Center, 3450 SW Campus Way, Corvallis, OR 97331 Debra Frederickson, Ph.D., Plant Pathologist, Texas A&M University, College Station, TX 77843 Gene Milbrath, Ph.D., Plant Pathologist, Oregon Department of Agriculture, 635 Capitol St. NE, Salem, OR 97301 Noe Montes, M. Sc., INIFAP Plant Physiologist, A. P. 172. Cd. Rio Bravo, Tam. Mexico. CP 88900. (Currently visiting research assistant at Texas A&M Expt. Stn., Corpus Christi) Jesus Narro-Sanchez, M.Sc., INIFAP Plant Pathologist, A.P. No. 112, Carr. Celaya-San Miguel de Allende km 6.5, Celaya, Guanajuato. CP 38000 Gary Odvody, Ph.D., Plant Pathologist, Texas A&M University, Route 2, Box 589, Agricul- tural Experiment Station, Corpus Christi, TX 78406 A complete copy of this manual is available at the Oregon Department of Agriculture website: http://www.oda.state.or.us Contents Introduction and overview to the genus Claviceps ................................................ 1 Life cycle ............................................................................................................... 2 Host range and distribution .................................................................................. -
Neuropsychedelia
Neuropsychedelia The Revival of Hallucinogen Research since the Decade of the Brain Nicolas Langlitz UNIVERSITY OF CALIFORNIA PRESS Berkeley • Los Angeles • London Contents. University of California Press, one of the most distinguished university presses in the United States, enriches lives around the world by advancing scholarship in the humanities, social sciences, and natural sciences. Its activities are supported by the UC Press Foundation and by philanthropic contributions from individuals and institutions. For more information, visit www.ucpress.edu. University of California Press Berkeley and Los Angeles, California University of California Press, Ltd. London, England © 2013 by The Regents of the University of California Acknowledgments Vtl Library of Congress Cataloging-in-Publication Data Introduction: Neuropsychopharmacology Langlitz, Nicolas, 1975-. Neuropsychedelia : the revival of hallucinogen as Spiritual Technology I research since the decade of the brain I Nicolas Langlitz. 1. Psychedelic Revival p. cm. Includes bibliographical references and index. 2. Swiss Psilocybin and US Dollars 53 ISBN 978-0-520-27481-5 (cloth: alk. paper) ISBN 978-0-520-27482-2 (pbk. : alk. paper) 3. The Varieties of Psychedelic Lab Experience I. Hallucinogenic drugs-Research. 2. Neuropsychopharmacology. 3. Hallucinogenic 4. Enacting Experimental Psychoses 2 drugs and religious experience. 1. Title. I3 BF209·H34L36 2013 - I54·4-dc23 5. Between Animality and Divinity I66 2012022916 6. Mystic Materialism 2°4 Manufactured in the United States of America Conclusion: Fieldwork in Perennial Philosophy 243 21 20 19 18 17 16 15 14 13 10 9 8 7 6 5 4 3 2 In keeping with a commitment to support Notes environmentally responsible and sustainable printing practices, UC Press has printed this book on 50-pound Bibliography Enterprise, a 30% post-consumer-waste, recycled, Index deinked fiber that is processed chlorine-free. -
Sclerotium Rolfsii; Causative Organism of Southern Blight, Stem Rot, White Mold and Sclerotia Rot Disease
Available online a t www.scholarsresearchlibrary.com Scholars Research Library Annals of Biological Research, 2015, 6 (11):78-89 (http://scholarsresearchlibrary.com/archive.html) ISSN 0976-1233 CODEN (USA): ABRNBW Sclerotium rolfsii; Causative organism of southern blight, stem rot, white mold and sclerotia rot disease 1Liamngee Kator, 1Zakki Yula Hosea and 2Onah Daniel Oche 1Department of Biological Sciences, Benue State University Makurdi, Nigeria 2Department of Medical Laboratory Science, School of Health Technology, Agasha, Benue State _____________________________________________________________________________________________ ABSTRACT Sclerotium rolfsii is a soil borne pathogen that causes stem rot disease on plants. It primarily attacks host stems including roots, fruits, petioles and leaves under favourable conditions. It commonly occurs in the tropics, subtropics and other warm temperate regions of the world. Common hosts are legumes, crucifers and cucurbits. On a global perspective, estimated losses of 10 – 20 million dollars associated with S. rolfsii have been recorded with yield depletion ranging from 1 – 60% in fields. Sclerotia serve as primary inoculum for the pathogen and are spread to uninfected areas by wind, water, animals and soil. Control measures include excluding the pathogen from the area, plant removal, soil removal, soil treatment, heat, solarization, chemical soil treatment, cultural practices, resistance and transgenic plant resistance, plant treatment, crop rotation, amongst others. Despite considerable research on this pathogen, its control continues to be a problem. Keywords: Sclerotium rolfsii, stem rot, white mold, stem blight. _____________________________________________________________________________________________ INTRODUCTION Sclerotium rolfsii is a destructive soil borne plant pathogen which causes Southern blight disease on a wide variety of plants. In 1928, the United States Department of Agriculture reported that S. -
Paleomycology of the Princeton Chert. II. Dark-Septate Fungi in the Aquatic Angiosperm Eorhiza Arnoldii Indicate a Diverse Assem
KU ScholarWorks | http://kuscholarworks.ku.edu Please share your stories about how Open Access to this article benefits you. Paleomycology of the Princeton Chert. II. Dark-septate fungi in the aquatic angiosperm Eorhiza arnoldii indicate a diverse assemblage of root-colonizing fungi during the Eocene by Ashley A. Klymiuk et al. 2013 This is the published version of the article, made available with the permission of the publisher. The original published version can be found at the link below. Ashley A. Klymiuk et al. (2013). Paleomycology of the Princeton Chert. II. Dark-septate fungi in the aquatic angiosperm Eorhiza arnoldii indicate a diverse assemblage of root-colonizing fungi during the Eocene. Mycologia 105(5):1100-1109 Published version: http://www.dx.doi.org/10.3852/13-025 Terms of Use: http://www2.ku.edu/~scholar/docs/license.shtml KU ScholarWorks is a service provided by the KU Libraries’ Office of Scholarly Communication & Copyright. Mycologia, 105(5), 2013, pp. 1100–1109. DOI: 10.3852/13-025 # 2013 by The Mycological Society of America, Lawrence, KS 66044-8897 Paleomycology of the Princeton Chert II. Dark-septate fungi in the aquatic angiosperm Eorhiza arnoldii indicate a diverse assemblage of root-colonizing fungi during the Eocene Ashley A. Klymiuk1 have been integral to the colonization of land by Thomas N. Taylor plants (Pirozynski and Mallock 1975, Humphreys et al. Edith L. Taylor 2010, Bidartondo et al. 2011); in the subsequent University of Kansas, Department of Ecology & , 450 million y, intricate associations have evolved, Evolutionary Biology; Biodiversity Institute, Lawrence, ranging from obligate mutualism through commen- Kansas 66045-7534 salism, parasitism and pathogenicity. -
Medicinal Mushrooms a Clinician’S Overview
Medicinal Mushrooms A Clinician’s Overview Dr. Christopher Hobbs Ph.D., L.Ac., A.H.G. • Licensed Acupuncturist (primary health care provider in California) • Clinical herbalist • Research Scientist, UC Berkeley • Author of Medicinal Mushrooms, other books on herbal medicine • http://www.rainbowlight.com/downloads/Medicinal_Mushrooms_C_Hobbs.pdf Mushrooms as Functional Foods • Popularity high in Asia, just beginning in Europe • North America—mushroom extracts sold without need of approval or registration • Most popular: shiitake, maitake, reishi, cordyceps, turkey tails; lion’s mane, chaga, Agaricus blazei • Products available: mushroom mycelium powder (grown on grain, soybeans), powdered extracts in capsules or tablets; ethanolic extracts (with or without glycerin Health Benefits of Mushrooms • Very little fat, no cholesterol • Valuable mineral content; high trace minerals • High in vitamins, especially B vitamins • Pleurotus provides a better supply of B vitamins than any common food, except meat • Great slimming food • Cholesterol regulation! Shiitake, Pleurotus • Very high in fiber—cellulose, lignan, chitin Mushroom as Food, Green Benefits • Highly nutritious—oyster mushroom is 25% high- quality protein; vitamins, minerals • Turkey Tails, Trametes versicolor, produces many enzymes used for detoxing toxic sites, biobleaching of paper, etc. Mineral Content of Fungi Mushrooms as medicine • Medicine— approved drugs in Japan, China for cancer treatment adjuvants (with chemo) • Health supplements to support immunity Biological Activity of -
Methoxetamine: from Drug of Abuse to Rapid-Acting Antidepressant ⇑ M
Medical Hypotheses 79 (2012) 504–507 Contents lists available at SciVerse ScienceDirect Medical Hypotheses journal homepage: www.elsevier.com/locate/mehy Methoxetamine: From drug of abuse to rapid-acting antidepressant ⇑ M. Coppola a, , R. Mondola b a Department of Addiction, ASL CN2, Viale Coppino 46, 12051 Alba (CN), Italy b Department of Mental Health, ASL CN1, Via Torino 70/B, 12037 Saluzzo (CN), Italy article info abstract Article history: Methoxetamine is a dissociative anaesthetic showing pharmacodynamic similarities with its analogue Received 3 May 2012 ketamine, a medication with demonstrated rapid-acting antidepressant effects. Like ketamine and other Accepted 5 July 2012 arylcyclohexylamine compounds, methoxetamine is thought to be both a noncompetitive NMDA recep- tor antagonist and a dopamine reuptake inhibitor. Furthermore, it acts as an agonist at dopamine D2, serotonin 5HT2, muscarinic cholinergic, sigma-1, opioid mu and k receptors. The hypothesis is that meth- oxetamine can produce rapid antidepressant effects in patients with resistant and non-resistant unipolar and bipolar depression. Ó 2012 Elsevier Ltd. All rights reserved. Introduction N-methyl-D-aspartate (NMDA) receptors produced antidepressant effects in animal models of depression [17,18]. In recent years, Mood disorders (MD) are chronic, recurring, disabilitating psy- some clinical studies have shown that an intravenous dose of ket- chiatric illnesses that affect millions people worldwide [1,2].In amine, an N-methyl-D-aspartate (NMDA) receptor antagonist, pro- particular, major depressive disorder (MDD) is a seriously disabling duces a rapid antidepressant response within hours [19]. These public health problem that produces severe psycho-physical and studies include the treatment of resistant BD in two double-blind, socioeconomic consequences in the population [3]. -
A Full Cycle of Mental Wellness from Research to Remedy
A Full Cycle of Mental Wellness From Research to Remedy CORPORATE PRESENTATION MAY 21, 2021 CSE:MCUR | OTC:MCURF | FRA:6MH Disclaimer & Forward Looking Statements This presentation (the “Presentation”) by MINDCURE Health Inc. (“MINDCURE”) is dated as of May 10, 2021. It is information or delivered. In this Presentation, forward-looking information includes, but is not limited to: statements pertaining to in a summary form and does not purport to be complete. It is not intended to be relied upon as advice to investors or the research, development and commercialization of technology, intellectual property or related products, or the timing potential investors and does not take into account the investment objectives, financial situation or needs of any particular thereof; the likelihood of success of any clinical trials; the likelihood of obtaining regulatory approval; the likelihood of investor. obtaining patents or the efficacy of such patents once granted; the leadership team; the potential for the markets that MINDCURE is anticipating to access; and the factors described under the caption “Risk Factors” in MINDCURE’s final INDUSTRY INFORMATION prospectus dated February 3, 2021 and documents incorporated by reference therein, and other documents publicly This Presentation does not constitute or form part of any offer for sale or solicitation of any offer to buy or subscribe filed by MINDCURE, which are available on MINDCURE’s profile at www.sedar.com for securities. This Presentation is not and under no circumstances is to be construed as a prospectus, offering memorandum, advertisement or public offering of any securities referred to herein, nor shall it or any part of it form Forward-looking information is not a guarantee of future performance and is based upon a number of estimates and the basis of or be relied on in connection with, or act as any inducement to enter into, any contract or commitment assumptions of management at the date the statements are made, including among other things, assumptions about: whatsoever. -
Occurrence of Coprophilous Agaricales in Italy, New Records, and Comparisons with Their European and Extraeuropean Distribution
Mycosphere Occurrence of coprophilous Agaricales in Italy, new records, and comparisons with their European and extraeuropean distribution Doveri F* Via Baciocchi 9, I-57126-Livorno [email protected] Doveri F 2010 – Occurrence of coprophilous Agaricales in Italy, new records, and comparisons with their European and extraeuropean distribution Mycosphere 1(2), 103–140. This work is the successor to a recent monograph on coprophilous ascomycetes and basidiomycetes from Italy. All Italian identifications of coprophilous Agaricales, which the author has personally studied over an 18 year period, are listed and categorized depending on the dung source. All collections were subjected to the same procedure and incubated in damp chambers and an estimate of occurrence of fungal species on various dung types is made. A second collection of Coprinus doverii is described and discussed, while the southern most finding of Panaeolus alcis is listed. An additional collection of Psilocybe subcoprophila, a species previously reported from Italy, is described and illustrated with colour photomicrographs. The morphological features of each species is briefly described, and substrate preferences compared with those reported from previous data. Key words – Coprinus doverii – damp chambers – fimicolous basidiomycetes – frequency – natural state – Panaeolus alcis – Psilocybe subcoprophila – survey. Article Information Received 25 March 2010 Accepted 21 May 2010 Published online 19 July 2010 *Corresponding author: Francesco Doveri – e-mail –[email protected] Introduction have recently been made and despite a The commencement of our systematic relatively slow increase in the numbers of studies on the dung fungi of Italy started in coprophilous basidiomycetes known from Italy 1992 resulting in Doveri (2004) and Doveri et and the inability to use field records for al. -
The Experiences of Kratom Users in Southern California
THESIS CALIFORNIA ST A TE UNIVERSITY SAN MARCOS THESIS SIGNATURE PAGE THESIS SUB1\.11TTEDIN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE MASTER OF ARTS IN SOCIOLOGICAL PRACTICE TITLE: The Experiences ofKratom Users in Southern California: A Sociological Explanation AUTHOR: Claudia Lacher DATE OF SUCCESSFUL DEFENSE: April 30, 2021 THE THESIS HAS BEEN ACCEPTED BY THE THESIS COM1\.11TTEEIN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE DEGREE OF MASTER OF ARTS IN SOCIOLOGICAL PRACTICE Christopher Bickel 05/07/2021 COM1\.11TTEE CHAIR SIGNATURE DATE Richelle Swan 05/07/2021 COM1\.11TTEEMEMBER SIGNATURE DATE Xuan Santos 05/07/2021 XoaIISaIItos(May 7,202113.371=e,f1 COM1\.11TTEEMEMBER SIGNATURE DATE COM1\.11TTEEMEMBER SIGNATURE DATE Table of Contents ACKNOWLEDGEMENTS ........................................................................................................... 2 ABSTRACT .................................................................................................................................. 4 INTRODUCTION ........................................................................................................................ 5 STATEMENT OF THE PROBLEM ............................................................................................. 6 LITERATURE REVIEW .............................................................................................................. 8 PHARMACOLOGY OF MITRAGYNA SPECIOSA ............................................................ 9 FROM EAST TO WEST: A Historical Tale of