Exploration of Cyanobacterial Flora from North Solaptehshil of Solapur
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FINAL REPORT OF MINOR RESEARCH PROJECT OF UGC Exploration of Cyanobacterial flora from North Solapur Tehshil of Solapur district(Mah) UNDER MINOR RESEARCH PROJECT Submitted to UNIVERSITY GRANTS COMMISSION WRO PUNE Submitted by Dr. M.N. Jagtap M.Sc.M.Phil.Ph.D. P.G. Department & Research centre of Botany D.B.F. Dayanand College of Arts and Science, Solapur. 413 002 SOLAPUR UNIVERSITY, SOLAPUR Annexure –III UNIVERSITY GRANTS COMMISSIONBAHADUR SHAH ZAFAR MARG NEW DELHI – 110 002. Final report Annual of the work done on the Minor Research Project. (Report to be Submitted within 6 weeks after completion of each year). 1. Project report No. 1st /2nd /3rd /Final -------- . Final 2. UGC Reference No.------ F-47-1427/10 (WRO) 22nd Sep 2010 3. Period of report: from 26th Dec 2010 to 26th Dec2012 19TH Nov 2010 -31st March 2013 4. Title of research project -------“ Exploration of Cyanobacterial flora from North Solapur Tehshil of Solapur district ( Mah)" 5. (a) Name of the Principal Investigator__ (i) Name : Dr. MHALAPPA NAMDEO JAGTAP (ii) Sex : Male (iii) Date of Birth : 01 – 06 – 1965 (iv) Qualification : M.Sc.M.Phil.(Botany)Ph.D.(Biotechnology) (v) Designation : Associate Professor in Botany (vi) Address Office : P.G. Department of Botany & Research centre D.B.F.Dayanand College of Arts & Science 2 Dayanand Nagar, Solapur (M.S.) 413 002 6. Effective date of starting of the project-------- 26th Dec 2010 7. Grant approved and expenditure incurred during the period of the report: a. Total amount approved Rs. 1,80,000 (One lac eighty thousand only) b. Total expenditure Rs . Rs. 1,80,000 (One lac eighty thousand only ) Report of the work done: (Separate sheet attached) ( i )Brief objective of the project _ 1. To evaluate the physico-chemical status of different types of agriculture soils and water of urban lakes . 2. Hydrobiological study of urban lakes . 3. To isolate nitrogen fixing cyanobacteria from soil and water . 4. To isolate toxic cyanobacteria from urban lakes 5. To isolate phytoplanktons from urban lakes. 6. To isolate heavy metal resistant bacteria from soil and water. iii. Work done so far and results achieved and publications, if any, resulting from the work (Give details of the papers and names of the journals in which it has been published or accepted for publication------------- 1. Jagtap M.N. ,M.V.Kulkarni and P.R.Puranik (2011) Accumilation Of heavy metals and concurrent physiological changes in Hydrophytes . International J. of Adv.Pharmacology.Toxicology 12(1)201141 -49. ISSN -0973-2381 3 2. Jagtap M.N.*, Dama L.B.**, Shaikh K.A.* and Shaikh H.G*. Isolation of Phytoplanktons from three urban lakes of Solapur city, Maharashtra state, India DAV International Journal of Science Volume-1, Issue-2 June, 2012 ISSN: 2277-5536 (Print); 2277-5641 (Online) 3. Jagtap M.N* Irkal M.R. ,Hirole R.G. Effect of BGA on Leguminous plant( Dolichos biflorus ) Proceedings of UGC Sponcered National Conference on “Recent Trends in emrging Infectious Diseases and Environmental Pollution” Organised by Department of Zoolgy D.B.F.Dayanand College of Arts & Sci Dayanand Nagar, Solapur (M.S.) 413 002 ISBN – 978 -81 -925586 -0 – 8 Page 64 -66 (iii) Has the progress been according to original plan of work and towards achieving the objective. if not, state reasons ---------- Yes (iv) Please indicate the difficulties, if any, experienced in implementing the Project : No (v) If project has not been completed, please indicate the approximate time by which it is likely to be completed. A summary of the work done for the period (Annual basis) may please be sent to the Commission on a separate sheet : NA vi. If the project has been completed, please enclose a summary of the findings of the study. Two bound copies of the final report of work done may also be sent to the Commission : Report attached. vii. Any other information which would help in evaluation of work done on the project. 4 At the completion of the project, the first report should indicate the output, such as (a) Manpower trained (b) Ph. D. awarded (c) Publication of results (d) other impact, if any_______1_Project to M.Sc. student and participated in Conference . 3. Residential address & e-mail : “Saket” A-15 Shrishail Nagar Bhawani Peth Solapur Solapur - 413002 [email protected] 5 Acknowledgement I extend my deepest sense of gratitude and express sincere thanks toV .K. Sharma Local Secretory Dayanand Institute Solapur , Dr. S. K. Vadagbalkar, Principal,D. B. F Dayanand College of Arts and Science, Solapur and Dr. S. N. Bawachkar, Head,Department of Botany, for permitting me to work and support in conducting the Project work. I am thankful to the non-teaching staff of the Botany Department, staff members at Administration Office, Accounts Department and Library for the co-operation extended during my project work. I am very much thankful to all my Colleagues, Graduate and Post Graduate students and my friends at D.B.F Dayanand College of Arts and Science, Solapur for providing much needed stimulus and support for successful completion of the project. I have no words to acknowledge my family members for the patience, encouragement and unconditional support bestowed on me all the time. Especially I can not forget the great understanding and strong back-up received from my wife Smita and daughter Sharayu, without which I could not have executed the research plan successfully. I sincerely acknowledge the financial assistance received from UGC, New Delhi to this Minor Research Project Above all I am grateful to the Almighty with eternal gratitude and humility for his blessings. 6 CONTENT Chapter 1 Introduction 12 Review of Literature 22 Chapter 2 20 Ecology and diversity of Cyanobacteria 38 Chapter 3 Hydrobiological study and isolation of 56 Chapter 4 phytoplankton from urban lakes. Chapter 5 Isolation and Characterization of 70 Metal Interacting Bacteria Chapter 6 BIOMASS PRODUCTIOON OF LOCAL STRAIN FOR AGRICULTURE 89 Chapter 7 Bibliography 102 Chapter 8 Summary and conclusion 109 7 List of Figures Fig. No. Figure legend Page 1.1 .Map of Solapur District 17 1.2 A Solapur District showing lacation of North Solapur Taluka 18 1.2B Solapur District showing lacation of North Solapur Taluka 19 1 .3 Study site north Solapur taluka 20 1.4 Map of Solapur city showing the study sites 21 3.1 Isolates on BG -11 Broth 46 3.2 Isolates on BG - 11 AGAR 46 3.3 Isolates from water 51 8 3.4 Isolates from water 51 3.5 Spirulina from Kambar lake 51 4.1 Lake sites 61 4.2 Phytoplankton diversity of three lake 66 4.3 Mcroscopic observation of Phytoplankton 68 4.4 Mcroscopic observation of Phytoplankton 69 5.1 Gradient plate method 79 5.2 Growth curve of bacterial isolates, MJ-Cr-6 and MJ-Pb-13 in 82 presence and absence of metals 5.3 3 Effect of pH on growth performance of the isolates in nutrient 83 broth 5.4 Growth performance of the isolate, MJ-Pb-13 in presence of 84 sublethal concentrations of metals (50 mg/l) 6.1 Biomass production Local strain 94 6.2 Physiocochemical characters of garden soil and BGA containin 95 soil 6.3 Isolation of local strain in bg-11 medium 96 6.4 Growth of Dolichos biflorus in pot 99 9 List of Tables Table Title of the table Page No. 2.1 28 Antifungal activity of cyanobacteria 2.2 . Cyanobacterial metal biosorbents 35 3.1 Geographical position of Sampling sites 42 3.2 43 To evaluate the physico-chemical status of different types of agriculture soils . 3.3 52 Characterization and identification of cyanobacterial isolates from soil/Water 3.4 53 .Characterization and identification of cyanobacterial isolates from soil /Water 3.5 Characterization and identification of cyanobacterial isolates 54 from soil /Water 4.1 . Lake Latitude / Longitude 59 62 4.2 Phytoplankton in three lakes in May2012 10 4.3 Table.4.3. Drinking water samples of WHO (1961), BIS (1991) 64 & ICMR 4.4 Physicochemical analysis of Water of three lakes. 65 4.5 Phytoplankton diversity of three lakes of Solapur city 66 5.1 Number of bacterial isolates obtained from metal enrichment 77 experiments 5.2 Growth performance of bacterial cultures in metal gradient 80 plate assay 5.3 Colony characteristics, morphology and Gram‟s nature of 80 bacterial cultures 5.4 Minimum inhibitory concentration of metals for selected 81 cultures in broth assay 5.5 Metal adsorption capacity of isolates at 100 mg/l initial 85 concentration of metals, pH 5.0 at 30C 6.1 physicochemical characters of garden soil and BGA containing 94 soil 6.2 Effect of two isolates on Dolochos biflorus 97 6.3 Effect of two isolates on Trigoneila foenum - graecum 98 11 Chapter 1 Introduction 12 Exploration of Cyanobacterial flora from North Solapur Tehshil of Solapur district(Mah) 1.introduction : 1.1 : Cyanobacteria Cyanobacteria are photo-autotrophic gram negative prokaryotes, also known as blue-green algae. They are the simplest known photo-autotrophic organisms having characteristics of both bacteria and algae and are one of the oldest life forms on earth dating back 3.5 billion years. They grow in a wide variety of habitats and > 2000 species are recorded with many new species still being discovered. Cyanobacteria exhibit three morphological forms, i.e., coccoid, unbranched filamentous and branched filamentous; and two functional groups, i.e., heterocyst bearing, nitrogen fixing, filamentous forms and non- heterocystous, non nitrogen fixing filaments. Although this description is generally true, many non-heterocystous species are known to fix atmospheric nitrogen. In aquatic habitats, cyanobacteria are regarded as harmful due to their association with environmental problems – fouling water bodies, blocking water supplies, unpleasant appearance and stinking odours, causing diseases and toxaemia etc.