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Downloaded from orbit.dtu.dk on: Oct 04, 2021 Occurrence of Cryptosporidium spp. oocysts in low quality water and on vegetables irrigated with low quality water in Kumasi, Ghana Petersen, Tobias B ; Petersen, Heidi H.; Abaidoo, Robert C.; Enemark, Heidi; Dalsgaard, Anders Publication date: 2014 Document Version Publisher's PDF, also known as Version of record Link back to DTU Orbit Citation (APA): Petersen, T. B., Petersen, H. H., Abaidoo, R. C., Enemark, H., & Dalsgaard, A. (2014). Occurrence of Cryptosporidium spp. oocysts in low quality water and on vegetables irrigated with low quality water in Kumasi, Ghana. Poster session presented at 5th International Giarda and Cryptosporidium Conference, Uppsala, Sweden. 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Acknowledgements The 5th International Giarda and Cryptosporidium Conference is supported by: Svenska föreningen för mikrobiologi (SFM) 4 Scientific Programme TUESDAY 27th MAY 13.00-18.00 REGISTRATION Uppsala Konsert & Kongress, conference venue 16.00-18.00 MINI-SYMPOSIUM: Childhood diarrhea - a global problem Chair: André Buret, University of Calgary, Canada O1 ” The Global Enteric Multicenter Study (GEMS) of Diarrheal Disease in Infants and Young children in Developing Countries”. Karen Kotloff, University of Maryland O2 ”The interaction of enteric infections and malnutrition: consequences for child health and development”. Bill Petri, University of Virginia O3 ”Cryptosporidium Strategy Development at the Enteric and Diarrheal Diseases Program, Bill & Melinda Gates Foundation” Ibrahim Khalil, Senior programme officer, Enteric Diseases, Bill and Melinda Gates Foundation Discussion 18.00-18.15 OPENING CEREMONY 18.30-21.00 WELCOME RECEPTION SciLifeLab Uppsala, BMC 6 Scientific Programme WEDNESDAY 28th MAY FOOD AND WATER TRANSMISSION Chair: Kristin Elwin (UK) 8.30-9.00 INVITED SPEAKER O4 Foodborne and waterborne transmission of Cryptosporidium and Giardia Lucy Robertson (Norway) 9.00-11.00 ORAL PRESENTATIONS O5 Essentials of environmental monitoring for Cryptosporidium and Giardia. Jerry Ongerth O6 A national strategy for human Cryptosporidium infection. Charlotte Axén, Ann-Christine Ring, Maria Egervärn, Torbjörn Lindberg, Linda Trönnberg, Jessica Beser, Anette Hansen, Martin Holmberg, Bo Svenungsson, Kaisa Sörén, Anna Nordström, Karin Troell, Jakob Ottoson and Annica Wallén Norell O7 μAQUA – a universal microarray for the detection of waterborne pathogens. Bas Boots, Ciara Ní Chualáin and Nick Holden O8 Selection of DNA aptamers for the detection of Cryptosporidium parvum in foods. Brent Dixon, Asma Iqbal, Darija Muharemagic, Maxim Berezovski and Syed Sattar O9 Wastewater treatment with Moringa oleifera seed extract: impact on turbidity and sedimentation of Cryptosporidium parvum oocysts. Heidi Petersen, Ian Woolsey, Anders Dalsgaard, Heidi Enemark and Annette Olsen O10 Investigation into a Cryptosporidium outbreak linked to a swimming pool. Elaine Moriarty, Debbie Smith, Anthony Pita, Nigel French and Ian Shaw 7 Scientific Programme O11 Swimming pool outbreaks of Cryptosporidium across England 1988 to 2012 Gordon Nichols, Rachel Chalmers and Adedoyin Awofisayo O12 An outbreak of Cryptosporidium parvum across England & Scotland associated with consumption of fresh pre-cut salad leaves. Gordon Nichols, Caoimhe McKerr, Goutam Adak, Russell Gorton, Rachel Chalmers, George Kafatos, Paul Cosford, Charlett Andre, Kevin Pollock and Stephen Morton 11.00-11.30 COFFEE BREAK (FIKA) 11.30-12.45 INFECTION IN ANIMALS AND ZOONOTIC ASPECTS Chair: Heidi Enemark (Denmark) ORAL PRESENTATIONS O13 Cryptosporidium infection and associated calf-level risk factors in French dairy calves. Carine Paraud, Christophe Chartier, Marie-Christine Dupuy, Mathieu Dumoulin, Isabelle Pors and Arnaud Delafosse O14 Cryptosporidium infection in beef calves in Sweden. Camilla Björkman, Lina Lindström, Carolina Oweson, Karin Troell and Charlotte Axén O15 Detection and molecular epidemiology of Giardia duodenalis and Cryptosporidium spp. in asymptomatic adult cattle in Northern Spain. David Carmena, Guillermo A Cardona, Begoña Bailo, José M Saugar, Carlos Marín and Isabel Fuentes O16 The role of adult cattle in the transmission and persistence of Cryptosporidium. Sarah Thomson, Beth Wells, Nicholas Jonsson, Elisabeth A Innes and Frank Katzer O17 Transmission dynamics of Cryptosporidium parvum between beef farms in two regions of Scotland. Emily J Hotchkiss, Sarah Thomson, Frank Katzer, Nicholas Jonsson and Elisabeth Innes 8 Scientific Programme O18 Age related susceptibility of pigs to Cryptosporidium scrofarum infection. Martin Kvac, Karel Nemejc, Michaela Kestranova, Dana Kvetonova, Pavla Wagnerova, Michaela Kotkova, Michael Rost, Eva Samkova, John McEvoy, and Bohumil Sak 12.45-13.45 LUNCH 13.45-14.45 INFECTION IN ANIMALS AND ZOONOTIC ASPECTS Chair: Camilla Björkman (Sweden) ORAL PRESENTATIONS O19 Giardia and Cryptosporidium in coyotes and wolves in northwestern Canada. Emily J Jenkins, Michael Pawlik, Mandeep Bal, Brent Dixon, Brent Wagner, Karen Gesy, Janna Schurer, Brett Elkin and Helen Schwantje O20 Occurrence of Giardia and Cryptosporidium in captive chimpanzees (Pan troglodytes), mandrills (Mandrillus sphinx) and wild Zanzibar red colobus monkeys (Procolobus kirkii). John J Debenham, Fred Midtgaard, Rebeca Atencia, Benezeth M Mutayoba and Lucy Robertson O21 Molecular characterization and genotyping of Cryptosporidium spp. in chicken and turkeys in Germany. Yosra A Helmy, Jürgen Krücken, Georg Von Samson-Himmelstjerna, Karl-H Zessin and Hafez M Hafez O22 Characterisation of Cryptosporidium in fish. Una Ryan, Andrea Paparini, Mikayla Morine, Melanie Koinari, Kaising Tong, Susan Keuh and Alan Lymbery O23 Identification of Cryptosporidium species in fish from the Leman Lake in France. Gabriela Certad, Jean Dupouy-Camet, Nausicaa Gantois, Ourida Hamouma-Ghelboun, Muriel Pottier, Karine Guyot, Sadia Benamrouz, Marwan Osman, Baptiste Delaire, Colette Creusy, Eric Viscogliosi, Eduardo Dei-Cas, Cecile-Marie Aliouat-Denis and Jérôme Follet 9 Scientific Programme 14.50-15.45 DIAGNOSTIC AND TREATMENT Chair: Kurt Hanevik (Norway) ORAL PRESENTATIONS O24 Implications of molecular detection on surveillance of intestinal parasites. Titia Kortbeek and Theo Mank O25 Introduction of a Giardia typing service in Wales UK). Kristin Elwin, Hannah Evans, Daniel Rh Thomas, Laia Fina, Sarah Jones and Rachel M Chalmers O26 A puzzling relationship between albendazole metabolization and albendazole resistance in Giardia duodenalis. Raúl Argüello-García, Maricela Cruz-Soto, Rodrigo Martínez-Espinoza, María L Bazán- Tejeda and Guadalupe Ortega-Pierres O27 GiTox: a simple colormetric method for screening drug susceptibility in Giardia duodenalis. Samantha J Emery, Jennifer H Gill and Ernest Lacey 15.45-16.15 COFFEE BREAK (FIKA) 16.15-17.20 ORAL PRESENTATIONS, CONT. O28 Elaboration of a vaccine to immunize pregnant cows to protect newborn calves from Cryptosporidium parvum infection. Karine Sonzogni-Desautels, Axel Renteria Flores, Momar Ndao, and Timothy Geary O29 Efficacy of chitosan, a natural polysaccharide, against Cryptosporidium parvum development in infected hct-8 and caco-2 enterocytic cells. Karim Adjou, Oleg Chihai, Alexandra Emmanuel, Veronique Laine-Prade, Aurelie Grasset-Chevillot, Jean-Philippe Marden, Eric Auclair and Isabelle Vallee 10 Scientific Programme O30 External quality assessment program for Cryptosporidium spp. diagnosis: experience of the French ANOFEL Cryptosporidium national network. Loic Favennec, Nathalie Kapel, Meja Radbodonirina, Jean Menotti, Claudine Sarfati, Claudine Pinel, Gilles Gargala, Francis Derouin, and French Cryptosporidium network O31 Evaluation of Cryptosporidium parvum infectivity by Electrochemical Impedance Spectroscopy. Jérôme Follet, Alfred DibaoDina, Alexis Vlandas and Vincent Senez O32 A novel method for simple and sensitive detection of Cryptosporidium parvum by surfactant extraction treatment (SET) and quantitative reverse transcription-PCR. Takahiro Sekikawa 17.30-19.30 WORKSHOP: Waterborne Cryptosporidium and Giardia: Regulatory and Legal Issues (See full programme on page 21) 11 Scientific Programme THURSDAY 29th MAY HOST-PARASITE INTERACTIONS AND PATHOLOGY - I Chair: Carmen Faso (Switzerland) 9.00-9.40 INVITED SPEAKER O33 Host-parasite interactions in giardiasis: from effects on microbiota to immunomodulation. Andre Buret (Canada) 9.40-10.20 ORAL PRESENTATIONS O34 Determinants of Cryptosporidium and Giardia virulence. (20 minutes) Kevin M Tyler, Maha Bouzid, Johanna Nader, Suha Al Naimi, Dong Xia, Audrey Dubourg, Kristin Elwin, Jonathan Wastling, Rachel Chalmers and Paul R Hunter O35 Representational difference analysis (RDA) identifies up-regulated genes in the murine intestinal epithelial cell line (IEC-6) in response to Giardia duodenalis infection. Showgy