Datasheet Version: 1.0.0 Revision date: 05 Dec 2020
Histone Acetyltransferase Type B Catalytic Subunit (HAT1) Antibody Catalogue No.:abx004728
Western blot analysis of extracts of various cell lines, using HAT1 antibody (abx004728) at 1/1000 dilution.
Immunohistochemistry of paraffin-embedded mouse cancer using HAT1 antibody (abx004728) at dilution of 1/200 (40x lens).
Immunofluorescence analysis of U2OS cells using HAT1 antibody (abx004728).
HAT1 Antibody is a Rabbit Polyclonal antibody against HAT1. The protein encoded by this gene is a type B histone acetyltransferase (HAT) that is involved in the rapid acetylation of newly synthesized cytoplasmic histones, which are in turn imported into the nucleus for de novo deposition onto nascent DNA chains. Histone acetylation, particularly of histone H4, plays an important role in replication-dependent chromatin assembly. Specifically, this HAT can acetylate soluble but not nucleosomal histone H4 at lysines 5 and 12, and to a lesser degree, histone H2A at lysine 5. Alternatively spliced transcript variants have been identified for thisFor gene. Reference Only
Target: HAT1
Clonality: Polyclonal
Reactivity: Human, Mouse, Rat
Tested Applications: WB, IHC, IF/ICC, ChIP
v1.0.0 Abbexa Ltd, Cambridge, UK · Phone: +44 1223 755950 · Fax: +44 1223 755951 1 Abbexa LLC, Houston, TX, USA · Phone: +1 832 327 7413 www.abbexa.com · Email: [email protected] Datasheet Version: 1.0.0 Revision date: 05 Dec 2020
Host: Rabbit
Recommended dilutions: WB: 1/500 - 1/2000, IHC: 1/50 - 1/200, IF/ICC: 1/50 - 1/200, ChIP: 1/50 - 1/200. Optimal dilutions/concentrations should be determined by the end user.
Conjugation: Unconjugated
Immunogen: Recombinant protein of human HAT1.
Isotype: IgG
Form: Liquid
Purification: Affinity purified.
Storage: Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.
UniProt Primary AC: O14929 (UniProt, ExPASy)
Gene Symbol: HAT1
GeneID: 8520
KEGG: hsa:8520
String: 9606.ENSP00000264108
Molecular Weight: Calculated MW: 39 kDa, 49 kDa Observed MW: 50 kDa
Buffer: PBS, pH 7.3, 0.02% sodium azide, 50% glycerol.
Concentration: 1.49 mg/ml Note: ForThis product Reference is for research use only. Only
v1.0.0 Abbexa Ltd, Cambridge, UK · Phone: +44 1223 755950 · Fax: +44 1223 755951 2 Abbexa LLC, Houston, TX, USA · Phone: +1 832 327 7413 www.abbexa.com · Email: [email protected]