Datasheet Version: 1.0.0 Revision date: 05 Dec 2020

Histone Acetyltransferase Type B Catalytic Subunit (HAT1) Antibody Catalogue No.:abx004728

Western blot analysis of extracts of various cell lines, using HAT1 antibody (abx004728) at 1/1000 dilution.

Immunohistochemistry of paraffin-embedded mouse cancer using HAT1 antibody (abx004728) at dilution of 1/200 (40x lens).

Immunofluorescence analysis of U2OS cells using HAT1 antibody (abx004728).

HAT1 Antibody is a Rabbit Polyclonal antibody against HAT1. The protein encoded by this is a type B acetyltransferase (HAT) that is involved in the rapid of newly synthesized cytoplasmic , which are in turn imported into the nucleus for de novo deposition onto nascent DNA chains. Histone acetylation, particularly of , plays an important role in replication-dependent chromatin assembly. Specifically, this HAT can acetylate soluble but not nucleosomal histone H4 at lysines 5 and 12, and to a lesser degree, histone H2A at lysine 5. Alternatively spliced transcript variants have been identified for thisFor gene. Reference Only

Target: HAT1

Clonality: Polyclonal

Reactivity: Human, Mouse, Rat

Tested Applications: WB, IHC, IF/ICC, ChIP

v1.0.0 Abbexa Ltd, Cambridge, UK · Phone: +44 1223 755950 · Fax: +44 1223 755951 1 Abbexa LLC, Houston, TX, USA · Phone: +1 832 327 7413 www.abbexa.com · Email: [email protected] Datasheet Version: 1.0.0 Revision date: 05 Dec 2020

Host: Rabbit

Recommended dilutions: WB: 1/500 - 1/2000, IHC: 1/50 - 1/200, IF/ICC: 1/50 - 1/200, ChIP: 1/50 - 1/200. Optimal dilutions/concentrations should be determined by the end user.

Conjugation: Unconjugated

Immunogen: Recombinant protein of human HAT1.

Isotype: IgG

Form: Liquid

Purification: Affinity purified.

Storage: Aliquot and store at -20°C. Avoid repeated freeze/thaw cycles.

UniProt Primary AC: O14929 (UniProt, ExPASy)

Gene Symbol: HAT1

GeneID: 8520

KEGG: hsa:8520

String: 9606.ENSP00000264108

Molecular Weight: Calculated MW: 39 kDa, 49 kDa Observed MW: 50 kDa

Buffer: PBS, pH 7.3, 0.02% sodium azide, 50% glycerol.

Concentration: 1.49 mg/ml Note: ForThis product Reference is for research use only. Only

v1.0.0 Abbexa Ltd, Cambridge, UK · Phone: +44 1223 755950 · Fax: +44 1223 755951 2 Abbexa LLC, Houston, TX, USA · Phone: +1 832 327 7413 www.abbexa.com · Email: [email protected]