General Meeting: SFMS at Merritt College Generalinside This Issue: Meeting 1/9 2 Nca Soc

San Francisco Microscopical Society Volume 8, #2 APRIL-MAY 2013

Renown English scientist Brian J. groups. We will meet at Merritt Ford, will be our speaker on College, 12500 Campus Drive, Wednesday May 15 meeting Oakland, in the Student Lounge, which we will share with other Building R, room 110 at 7:30. organizations including the Park on the west side of the Northern California Society of campus ($2.– in quarters). Microscopists and several other Refreshments will be served.

INSIDE General Meeting: SFMS at Merritt College GeneralInside this issue: Meeting 1/9 2 NCa Soc. for Micro 2 Wednesday 7:30 PM, May 15, 2013 Board Meeting 3/17 2

Bio: Mary Ann Scott 3 Please see the infor- in America for his work in mation above which is not microscopy. Dr Gonzales-Edsinger 4 repeated here. We have invited him to If you have had the talk about the astonishing iFuse :Sci. Exploration 4 pleasure and privilege of views that primitive micro- hearing Brian Ford in the scopes presented to past Bio: Henry Schott 5 past, you know he always scientists. surprises his audience with Brian takes us back to Pesky Protozoan ID 5 his insightful presentations visit the Netherlands in the Internet Connections 6 on science and microscopy 1600s and also to London. Join SFMS 7 in particular. Where Leeuwenhoek and Volunteer for 4/27/13 8 He was presented with Hooke gave birth to the sci- THIS SATURDAY the inaugural Köhler medal ence of microscopy. Profes- BRIAN J. FORD Page 2 San Francisco Microscopical Society Volume 8, #2 April May 2013 GENREAL MEETING, January 9 Northern California BOARD MEETING ELECTION OF OFFICERS & PRESENTATION by Dr. Eric Gonzales Society for Microscopy MARCH 17, 2013 NCSM INFORMATIION Dr Eric Gonzales-Edsinger is a High lights of the board meet- postdoctoral researcher in Daniel You are invited to our next ing reflect the effort that the at- Rokhsar's lab at both UC Berkeley chapter meeting. The evening tending members invest in the and OIST. will include socializing, business Dr Eric Gonzales describes and discussions, a light meal and sci- Society. Peter reported on his summarizes his research interests as entific presentations. If you have effort to secure the Merritt Col- follows: not registered for membership lege Student Lounge as the venue into this local affiliated chapter of for Brian Ford’s presentation on "My research is on the ge- the Microscopy Society of Ameri- nomics, development, and evolution ca you can obtain an application THE FIRST MICROSCOPES—WHAT of marine invertebrates, including form from the web site. Member- COULD THEY TRULY REVEAL? Come gastropod and cephalopod molluscs. ship is $40 per calendar year. to the meeting, Wednesday, May I'm interested in the evolution of The Northern California Soci- animal body plans, biomineralization, ety for Microscopy is a sanc- 15, See the front page article. cilia, nervous systems, and transpar- tioned, local affiliate society (LAS) Bug Day is a big celebration ency. I am also interested in next- of the Microscopy Society of generation imaging technologies, America. Sponsorships of NCSM at the Randall Museum in San such as light-sheet microscopy, and are available for your company or Francisco and we have a booth in the development and integration organization; please contact me where we help young people see of metazoan anatomical and lifecycle directly if you are interested in the fine buggy details. WE NEED ontologies with the Tree of Life and making a donation as these funds hypotheses of homology. The initial are needed to support chapter YOU THERE to help from 10 to focus of my postdoctoral research is activities. We are also in need of two in the afternoon on Satur- on species selection and develop- your help in running this chapter day, April 27. Call Myron or Pe- ment of a model system for cephalo- and welcome your participation. ter 415 585 4747 or 415 261 7114. pods, most likely from within the The meeting in March includ- pygmy squids and/or octopuses." ed these speakers: You will enjoy it & get a free lunch. “At Merritt College, we have Michael Isaacson, PhD. Narinder The board recognizes the Singh Kapany Professor, Direc- been involved in a student project need to improve our web site using widefield and confocal fluores- tor, Center for Sustainable Ener- and will consider how much to cence imaging to visualize the devel- gy and Power Systems, Jack opmental stages in embryos of lim- Baskin School of Engineering invest in this effort. Your input is pet snails from several species of the University of California at Santa needed to tell board members Cruz: "Microscopy Through the genus Lottia. By understanding the what you would like to see on developmental biology of these spe- Centuries: An Exploration of cies and comparing them to those of Microscopic Imaging without our web site. Lenses" other mollusks, the idea is to better MEMBERSHIP stands at 45 of understand the evolutionary devel- Danielle Jorgens, graduate which 24 are life members. Six opmental biology of this group. student, Lawrence Berkeley Na- Comparative developmental biology tional Laboratory and Oregon 2012 members have not paid ("evo-devo") has emerged as a key Health & Science University: their dues. If you read this and area in understanding evolutionary "Seeing cells in 3D: a tale of multi-modal imaging" want to pay, but are uncertain if biology.” Ailey Crow, Image Acquisition you have paid, look for the His presentation was very well and Analysis Specialist, Genen- stamped envelope we sent you received and we saw some excellent tech Inc.: illustrations of his work. The meet- or call Myron, our treasurer. (Continued on page 3) ing was well attended. (Continued on page 3) Page 3 Volume 8, #2 April May 2013 San Francisco Microscopical Society

Who is Mary Ann Scott? First in a series To Know Your Members. office clerk. She was an intern at UCSF Hel- Mary Ann Scott is our near Mt. Shasta, Ca. returned to her first love en Diller Cancer Cen- newly elected Recording where she took a liberal of ballet and studied seri- ter where she per- Secretary and a member arts course and was a ously for 25 years. She formed experiments of the board. This posi- member took clas- that focused on cell tion requires taking notes of the ses with growth and prolifera- at our board meetings National the New tion. Specifically, Rheb and producing the Ski Pa- York City was targeted to discov- minutes of these meet- t r o l . Ballet and er how it affects the ings. She also describes M a r y d a n c e d growth and prolifera- what occurred at our A n n p r o f e s - tion in cancer and tu- regular meetings so that t r a n s - sionally in mor cells. Mary Ann readers of Micro News ferred to the East was attracted to mi- will have at least some C o l l e g e Bay. Mary croscopy from memo- idea of the topics dis- of Idaho Ann also ries of her first micro- cussed. This is not an w h e r e lived in scope and the love of easy task. she con- Sun Valley, small creatures. She Mary Ann Scott was tinued a I d a h o earned a Certificate in born in Berkeley and l i b e r a l where she Bioscience Microscopy grew up in Alameda, Ca. a r t s worked as from Merritt College She spent her childhood course majoring in Sociol- a nanny, and as a teach- and she took a course enjoying camping in Wyo- ogy and Psychology. After er’s aide and a dental of- in SEM from Ohlone ming and Idaho, skiing and graduation (BA Sociology) fice clerk. College. She is continu- ballet lessons. Mary Ann she worked as a co- In 2007 after returning ing her education at studied entomology as a manager of a bookstore to the Bay Area she re- San Francisco City Col- child, and loved her first in Pullman, Washington, turned to school focusing lege in the Stem Cell and took a graduate on math and science. She Program. She owns 2 microscope. microscopes, a com- She went to college course. Mary Ann then then worked as a dental

Biosciences Division (Continued from page 2) (Continued from page 2) ner made a presentation of SRI International, Inc. MUSHROOMS UNDER "Angiogenesis: Imag- 333 Ravenswood Ave. The IRS refunded SFMS THE MICROSCOPE at the ing & Image Analysis" Menlo Park, CA 94025 For additional infor- (650) 859-2980 office $700.– that we paid as part March 13 General Meeting. mation: in- (650) 859-3153 fax of the 501(c)3 application His interesting PowerPoint [email protected]. process. That was nice of show and actual demon- our government. Thank stration of slide making was Best regards, you. well attended and received Steve Samuelsson by a roomful of attendees. Treasurer, NCSM We own 55 shares of US Microscope Company , One grant was funded Steven J. Samuelsson, Ph.D. Inc. but are unable to de- at $3,229.83 to David Principal Scientist termine if they have any Armitage at UC Berkeley. Director, Cell and Mo- value. Any ideas? lecular Imaging (Continued on page 7) 100-51 President Peter Wer- Page 4 Volume 8, #2 April May 2013 San Francisco Microscopical Society

Presentation by Dr. Eric Gonzales Edsinger on January 9, 2013 sheet and light-sheet based functional tools President Peter Werner and Cephalopods Ge- of light sheet microscopy provide a method for called the meeting to or- nomics and Develop- that uncouples the illumi- capturing live imaging der at 7:15 pm and after ment. nation and detection axes throughout the embryonic life cycle of an some opening remarks Dr. Edsinger began his in an optical set up allow- organism because there introduced Helmut Will, a talk dedi- ing im- is no photo toxicity life member, who ran the cated to aging of with light-sheet illumi- election of officers sched- new types biologi- nation. Instead of verti- uled for each January. of micros- cal spec- cal illumination, light- Nominations from the copy: con- imen sheet illumination is floor were encouraged focal and with low introduced horizontally. and the slate of officers light sheet photo Zeiss came out with the introduced. Ballots were and open damage. first version. Eric then passed out to 13 members source OMERO showed video with present and the count in- storage of microscopy handles all images in a Drosophila and Zebra dicated that the slate was data. secure repository. Micros- elected unanimously. The copists and scientists can Fish heart examples of Open source microsco- time lapse with xyz officers are recorded else- py includes Open SPIM view, organize, analyze where in this issue. (See and share data from any- cameras capturing im- and OMERO. Open SPIM ages in real time. report of the Board Meet- (Selective Plane Illumina- where that has internet The anatomy of a 15 ing 1/24/13) tion Microscopy) is an access. He then turned to hour embryo of the sea President Werner intro- open access project to the new light-sheet mi- snail Lottia gigantea duced Dr. Eric Edsinger build an accessible entry croscopy and compared it was illustrated and ex- who gave a talk on Imag- level implementation of with confocal microscopy. (Continued on page 6) ing Data in Gastropods SPIM. SPIM is variation New advances in light-

until they are in a middle to illuminate young minds, understanding of a world i F u S E school science class unless principally microscopes. JH invisible to the unaided they are fortunate to end Technologies refurbishes eye. Children, at the age up in a classroom that has microscopes that it takes in Science education has when they can explore and benefitted from the iFuSE trade when it sells new sets suffered severely in part give in to their natural curi- program developed by JH and provides them, free of due to the cost of good osity, are easily intrigued by Technologies. Inc. of San charge to K though 12 equipment and the un- what the microscopic world Jose. schools that cannot afford willingness of teachers to offers. While most parents to buy this equipment. The acronym, when use available resources. will expose them to a mag- deciphered, reveals that the As a result, students nifying lens, most children For more infor- intent is to inspire Future under the leadership of will not have an opportunity mation about iFuSE Scientific Exploration by skilled teachers develop to see real objects magnified email: providing teachers the tools investigative skills and an between 10 and 100 times [email protected] Page 5 San Francisco Microscopical Society Volume 8, #2 April May 2013

KNOW YOUR BOARD MEMBERS Henry Schott, Communicating Secretary & Editor, Micro News

Everyone starts some- he returned to San Francisco to where in life and most likely earn a Master in Biology and a travels some distance from secondary teaching credential where they are born so it from SFSU. should come as no surprise that He began his teaching ca- Henry was born as Enrico, in reer at San Leandro High Henry Schott preparing a demonstration during a workshop. Milan, Italy, in 1928. As he School where he remained for likes to say “ I was born in eight years before joining the Milan because that is where my He and first wife had three public libraries. As a life faculty at Merritt College in mother was.” It is also where children and now they have five member of SFMS, Henry 1964 with the newly formed his father and brother were. grandchildren. has served as Vice President Peralta Community College & Program Chair, President, Both parents were German and He and his second wife, District. His interest in micros- Treasurer, and since April, his family lived in Italy be- Marilyn Little who is a Ph.D. copy, which UC and SFSU had 2007, as editor of the Micro cause his father conducted his in Sociology, and is also retired, nurtured, was given a boost by News. He owns three micro- business there. started a business representing the large slide collection that scopes and a slide collection companies that produced in- Forced out of Italy by a Merritt had available for stu- of human pathology but structional CDs. He traveled all Fascist law that passed in 1938, dents but it was Henry’s inter- finds greater interest in over the west coast visiting his family moved first to Eng- est in making use of every recognition and understand- community colleges and univer- land and then, in 1940 to available visual aid in both ing of normal tissues. Berkeley, California where he lecture and laboratory that sities. When this became un- attended Berkeley High School helped his students learn anato- profitable they focused on real He is also involved in and then UC at Davis and my and physiology. He particu- estate. the ecology and plant resto- ration projects of the Friends Berkeley. larly liked pointing out the re- It was several years after he lationship of cellular structure of the Montclair Railroad After serving in the US retired when he first became to physiological processes, an Trail. He serves on their Army (Veterinary Corps) dur- aware of the SF Microscopical area that for him continues to board of FOMRRT as treas- ing the Korean war and work- Society. Some meetings were be intriguing. urer. ing in NY for almost two years, held in Oakland at one of the

FREE FOR THE PESKY PROTOZOAN zoa, a monumental task. I asked him to describe ASKING All single celled organ- his identification proce- TO MEMBERS: IDENTIFICATION isms are interesting since dure. Here is his response: Donated by W. they package all the func- Gurske, a large col- We are trying to im- tions of life in one small “When I try to identi- lection of unidenti- prove our web site and are package and manage, in fy an organism I first refer fied pathology and looking for ideas that we normal human tis- their great diversity, to to 2 books that I have at sue slides now in can address. Since we occupy a very wide range home, How To Know Pro- the hands of Henry have funds to invest in of niches. tozoa by Theodore L. Schott. You can have up to 10 slides this project, any sugges- William Gurske takes Jahn, Eugene c. Bovee, tions you may have would for exploration, great pictures of small and Frances F. Jahn 2nd practicing patholo- be of interest to the board. organism and has demon- Ed. 1979 and An Illustrat- gy, histology, or just microscopy. Staining One suggestion want- strated his set-up at past ed Guide To The Proto- ed the board to consider is very good. meetings. He avidly tries zoa 2nd Ed. Edited by hschott@aol,com developing a database for to identify what he sees so the identification of proto- (Continued on page 6) Page 6 Volume 8, #2 April May 2013 San Francisco Microscopical Society

(Continued from page 5) www.edge-3d.com Lists the following: Links to Other Websites and Resources John J. Lee, Gordon F. Relating to Microscopes and Microscopy. Leedale, and Phyllis Bradbury, Society of Pro- tozoologists 2000, ISBN http://www.rms.org.uk -"The Royal Microscopical Socie- No. 1-891276-22-0, 2 ty." Volumes. The first book http://www.msa.microscopy.com/ -"To promote and to is for amateurs and the 2 communicate advances in microscopy." volumes from the Society http://www.microscopy-online.com -"Resource for the of Protozoologists are at a microscopist." graduate level. When I http://www.biofx.com -"High sensitivity immunosub- think I have identified the strates, unique conjugate labeling techniques, and im- organism by family, or munosolutions." order, or genus, I then www.buntgrp.com -"Bunton Instrument Co. - Analog and Google the name and look Digital 3D viewing and recording solutions for use on ste- at sites such as, eol.org, Encyclopedia of Life. I reo/surgical microscopes." do not know of a single http://www.amc.anl.gov/docs/NonAnl/ComSites.html - database for microscopi- "Microscopy and microanalysis." cal organisms. http://www.vaytek.com -"Scientific Image Processing The 2 volume guide systems, Deconvolution, 3D rendering." on Protozoa is excellent http://www.DTI3D.com -"Dimension Technologies Inc.- for classification of proto- the 3D technology specialists" zoa, but an amateur will http://www3.bc.sympatico.ca/micron/microscope/ struggle with the descrip- links1.htm -"Extensive microscopy links page." or tive terms used in the two http://www3.telus.net/micron/microscope/links1.htm volumes.” http://www.bioresearchonline.com -"Digital market- Best regards, place for the bioresearch and life sciences industry." Bill (William Gurske) [email protected]

(Continued from page 4) Dr. Edsinger ent with DIC microscope that there is intense swim- on the self–assembly and technique and therefore ming behavior before the function of the cytoskele- plained. Lottia larvae can do not reveal internal onset of the nervous sys- ton during embryonic cell be cultured in the labora- anatomy. tem formation at 30-36 division especially during tory and followed thru Next came images of hours. Swimming videos the induction and mainte- metamorphosis. Adult classical developmental of Lottia embryos at dif- nance of cytogenetic ap- Lottia do not spawn in the stages now available in ferent hours of develop- paratus – the poorly un- lab so must be gathered digital atlases. Research ment were shown as ex- derstood machine that from the ocean. Eric based on Lottia swim- amples of the various pinches the cell in two. showed examples of Lot- ming behavior completed swimming behaviors. The complex gene se- tia larvae at 15 hours with by a group of Merritt Col- Mentioned also, was quence of octopus devel- DIC SEM and Confocal. lege students was intro- the research of George opment formed the sec- Embryos are not transpar- duced. It was determined Von Dassow that focused (Continued on page 7) Page 7 San Francisco Microscopical Society Volume 8, #2 April May 2013 sor Ford has recently succeeded—for the first time in history--in obtaining Join SFMS videos using digital image-capture that recreates precisely what was MEMBERSHIP seen by microscopists in the 1600s. His presentation on this research to INFORMATION the Royal Society of London was a To join the Society: memorable occasion, and today he shows further images from the dawn Fill in the form available on our of microscopy in this vivid presenta- web site www.sfmicrosoc.org tion. after printing it. Make check out The lecture is titled: THE FIRST MICROSCOPES — WHAT COULD THEY to SFMS. TRULY REVEAL? Dues are $12. /calendar year, or The scientific journal Nature re- $144.– for Life Membership. ported: “Ford is the world’s leading expert on the topic, and what he has to say here makes a good deal of sense.” Mail it to : You are encouraged to come no SFMS Treasurer, Myron Chan later than 7:15 so that you have time One of the many books published 435 Melrose Ave to refresh yourself and meet your by Brian J. Ford. For more infor- friends and colleagues. HS mation, go to his web site. San Francisco, CA 94127

http://www.brianjford.com/ bjford.htm

(Continued from page 6) (Continued from page 3) to the society by offering to serve. You need only contact one of the of- ond part of Eric’s presentation. A report will be provided in the next ficers to let them know your interest. The octopi are Cephalopods as are issue. A second grant is pending but Call 415-261-7114 or 415 - 585-4747 the squids and cuttlefish. He spoke the board decided to postpone any or 510-339-9609 or 510-523-4132. about the smallest, fastest, and action until it is fully satisfied that the Your interest will be much appreciated funds will be properly used. and you will benefit the society and smartest octopus. The Pygmy yourself through this experience. Squid and Pygmy Cuttlefish are At the Board Meeting, it was de- the smallest, Pygmy Octopus and cided to postpone any new grants and At the time of this printing, the Thaumoctopus mimicus show the not send out Requests for Proposals time and location of the next board most intelligence. Idiosepius para- until we have had a chance to review meeting is May 5, Sunday, 20 Drake and better organize the entire process. Lane, Oakland at noon for lunch and doxus were collected in Nagoya, This can be interpreted as skipping the 1-3 PM for the board meeting. Come! Japan, for development of imaging 2013 Requests for Proposals. We If you have concerns or ideas that protocols back in UC Berkeley. need member volunteers to oversee you would like to bring to the atten- Eric concluded his talk by adding the process and make recommenda- tion of the board, please address them that O. bimaculoides is the easiest tions to the board. to the President and send them by e- octopus to develop in the laborato- The imminent resignation of one mail or USPS to [email protected] ry. of the board members creates an op- Prepared by Mary Ann. Scott portunity for a member to contribute Page 8 SAN FRANCISCO MICROSCOPICAL SOCIETY

Stamp SFMS

Volume 8, #2 April May 2013

FROM: Micro News San Francisco Microscopical Society 20 Drake Lane Oakland, CA 94611-2613 TO:

We’re on the Web!

example.com

{WE} Bug {YOU} Day at the Randall Museum

April 27, 2013 Saturday, 10 to 2:00 PM

This does not sound like an invitation but it is to the Mu- seum's annual festival of bugs! Our participation in this program is one way we are able to repay the Museum for the use of their facility. It is also a public service that we take pride in providing. Kid of all ages love looking through microscopes at wings, eyes and legs of insects as well as seeing some aquatic insects scurrying about. Your volunteer participation in our booth is therefore requested. Call Myron, (415) 585-4747 or show up early to help set up the booth. If you work, you get a free lunch courtesy of the Museum. Bugs magnified may be the first experience at en- [Free admission (donations appreciated), free and low-cost activi- joying microscopy for this youngster. Our micro- ties for all ages. If you join Randall Museum Friends on Bug Day at scopes in use at the Randall Museum. the Family level of $45 we will give you a free bug cap!]