IOSR Journal of Biotechnology and Biochemistry (IOSR-JBB) ISSN: 2455-264X, Volume 3, Issue 5 (Sep.- Oct. 2017), PP 11-18 www.iosrjournals.org Genetic diversity of Muscovy ducks revealed by mtDNA d-loop Kameshpandian Paramasivam1, Satyanarayana Swamy Vyshnava2, Dileep Kumar Kanderi2, Cino Pertoldi3,4. 1 (Department of Genomic Science, Central University of Kerala, Kasaragod, Kerala, India,
[email protected]) 2 (Department of Microbiology, Sri Krishnadevaraya University, Anantapuramu, AP, India) 3 (Department of Chemistry and Bioscience, Aalborg University, Aalborg, Denmark) 4(Aalborg Zoo, Aalborg, Denmark) Abstract: Based on geographical location and previous studies, the Muscovy ducks were grouped into six different populations such as India-Manipuri (IM), Wuyi-China (WC), Yuyao-China (YC), Fujian-China (FC) and Unkown-China (UC) and France. In total, 12 haplotypes were observed from six Muscovy population. The India-Manipuri (IM) population contributes 8 haplotypes. The AMOVA test shows high (75.12%) genetic variation within population. The NJ phylogenetic tree shows the intermingled China, India and France Muscovy populations. In order to find the depth of haplotype differences, the median joining network was constructed whereby H1 haplotype were shared by India and China population. Moreover, except H1 haplotype, other seven haplotypes from IM population were not observed to be shared with China population. Most importantly 33 InDel sites were observed, with regard to that another MJ network was constructed based on the InDel (Insertion-Deletion) sites or including alignment gaps. This InDel MJ network shows that India and China haplotypes separation based on H5 and H1 haplotype, and star-like structure also observed in both the networks.