獣医臨床皮膚科 21 (2): 63–69, 2015

Original

The Signifi cance of the Numbers of Mast Cells in the Evaluation of Skin-Biopsy Specimens from Cats with Infl ammatory Dermatoses 炎症性皮膚疾患の猫から採取した生検皮膚の評価における 肥満細胞数の有用性

Maturawan Tunhikorn1)*, Danny W. Scott1), Hollis N. Erb2) 1)Departments of Clinical Sciences and 2)Population Medicine and Diagnostic Sciences, College of Veterinary Medicine, Cornell University

Received January 6, 2015 and accepted February 12, 2015

Abstract: It has often been stated that large numbers of mast cells in skin-biopsy specimens from cats are consistent with allergic dermatitis. We performed a retrospective study of hematoxylin and eosin (H&E)- stained skin-biopsy specimens from cats with allergic (n=143) and nonallergic (n=228) infl ammatory skin diseases. All specimens had been submitted to the Section of Anatomic Pathology of the Cornell University College of Veterinary Medicine between 1978 and 2010. We found that dermal numbers are not signifi cantly different between allergic and nonallergic cats. We also found that mast cell numbers are higher in the superfi cial than the deep dermis in cats with allergic and nonallergic skin diseases. In normal cat skin (n=31), mast cell numbers in the superfi cial dermis were higher in toluidine blue-stained specimens than in H&E-stained specimens. Key words: cat, infl ammatory dermatoses, mast cells, skin

要 約：猫の生検皮膚中に多数の肥満細胞がみられた場合，アレルギー性皮膚炎に矛盾しない所見 であるとしばしば述べられている。今回，著者らはアレルギー性皮膚炎（ 143 例）と非アレルギー 性炎症性皮膚疾患（228 例）に罹患した猫から採取され，ヘマトキシリン & エオジン（H&E）染色 を施した皮膚生検標本を用いた後向き研究を実施した。本研究に用いた検体の全ては， 1978 年か ら 2010 年までの間にコーネル大学獣医学部解剖病理学部門に送付されたものであった。アレルギー 性皮膚炎の猫と非アレルギー性炎症性皮膚疾患の猫との間で，真皮における肥満細胞数には有意差 が認められなかった。またアレルギー性皮膚炎または非アレルギー性炎症性皮膚疾患のいずれに罹 患した猫でも，肥満細胞数に関しては真皮浅層における数の方が，真皮深層における数よりも高値 を示した。健常猫（31 例）で調査したところ，真皮浅層における肥満細胞数は，トルイジンブルー 染色を施した標本における数の方が，H&E 染色を施した標本における数よりも高値を示した。 キーワード：猫，炎症性皮膚疾患，肥満細胞，皮膚

(Jpn J Vet Dermatol 2015, 21 (2): 63–69)

Introduction

* Correspondence to: Maturawan Tunhikorn (Department of The role of mast cells in the cutaneous immune Clinical Sciences, College of Veterinary Medicine, Cornell 4, 5, 10) University), Ithaca, New York 14853-6401, USA system of cats has been reviewed . Mast cells are FAX +1-607-253-3534 E-mail: [email protected] normal residents of connective tissue, and are found in 64 Mast Cells in Feline Infl ammatory Dermatoses

highest numbers in regions of the body that interface Materials and Methods with the environment: skin, lung, and gastrointestinal tract4, 5, 12). In addition to their antigen-specifi c role in the A retrospective study of skin-biopsy specimens from development of allergic infl ammation in the skin, mast 371 cats with infl ammatory dermatoses was performed. cells are important in the innate defense against bacteria, All biopsy specimens had been submitted to the Section and in wound healing4, 5, 10). of Anatomic Pathology at the College of Veterinary Small studies of hematoxylin and eosin (H&E)-stained Medicine at Cornell University betw