Zhongyuan (Zach) Zuo 4510 Camden Brook Ln Cellphone: 970- 366- 0744 Katy TX, 77494 Zhongyuz@Bcm.Edu

Zhongyuan (Zach) Zuo 4510 Camden Brook Ln Cellphone: 970- 366- 0744 Katy TX, 77494 [email protected]

Education

University of Denver, Biological Science Department 08/2012-06/2014 MS Molecular Biology Denver, CO. USA GPA: 3.83

University of Denver, Biological Science Department 09/2008-06/2012 BS Molecular Biology Denver, CO. USA Minor: Mathematics, Chemistry, Medical Physics GPA: 3.78 (Cum Laude)

Skills: Spinning disk and laser confocal microscopy, PCR and molecular cloning, SDS/ Western blotting, Teaching undergraduate biology level class, DIC/Polarized light microscopy, Protein chromatography, antibody purification, histology processing, ultra-microtomy, and Transmission Electron microscopy. Member of Toastmasters International with an aptitude for public speaking.

Working Experiences

Research Assistant I 2018—present Molecular and Human Genetics – Baylor College of Medicine  Biological sample preparation for transmission electron microscopy  Performed ultra-microtomy  Applied histology H&E staining for animal samples  Captured images on a JEOL 1010 transmission electron microscopy  Confocal and bright-field microscopy image acquiring and analyzing on Zeiss LSM 710, 880, Nikon W1, and Leica SP8 laser scanning confocal microscopes  Manage and repair laboratory equipment  Perform routine laboratory server maintenance and update

Transmission Electron Microscopist /Research Technician III 2014—2018 Molecular and Human Genetics – Baylor College of Medicine  Biological sample preparation for transmission electron microscopy  Performed ultra-microtomy  Applied histology H&E staining for animal samples  Captured images on a JEOL 1010 transmission electron microscopy  Confocal and bright-field microscopy imaging on Zeiss LSM 710, 880, and Leica SP8 laser scanning confocal microscopes

Molecular and Developmental Biology Research/ Teaching Assistant 2012—2014 Biological Science Department - University of Denver  Performed molecular cloning and genetic crosses  Performed live imaging-Spinning disk microscopy  Applied immunofluorescence microscopy for fixed embryonic tissues  Performed germline (FLP-FRT) knockouts and drug injection experiments  Developed a novel embryo dissecting and squish movie technique  Teaching assistant in General Genetics and Cell Biology

Cell Biology Lab Assistant 2011—2012 Biological Science Department - University of Denver  Researched with Dr. Snyder related to the cytoskeleton in ptK1cells  Used different drugs that bind to microtubules to try to cease reproduction in cancer cell

SERI (The Scientific Education and Research Institute) Medadvance Internship 2010—2011 The Scientific Education and Research Institute  Organized cadaver dissections and shadowed doctors  Observed a variety of surgeries  Assisted at physician training courses, gained exposure to cutting edge technology and interaction with doctors and surgeons from across the world

Undergraduate Teaching Assistant Chemistry Department- University of Denver 2010—2012  Held lab session of General Chemistry and Analytical Chemistry  Prepared experiment supplies and materials  Instructed students with accurate titration and detailed measuring techniques  Certified Organic Chemistry Tutor

Physics Department - University of Denver 2011—2011  TA for Dr. Iona in a physics class for non-majors

Mathematics Department - University of Denver 2009—2009  Helped students with questions related to College Calculus I, II, and III  Held small group reviewing session before midterms and finals  Gave advices on their lab reports and answered questions about the software “Scientific Notebook”

Activities

Toastmaster International-Denver DayBreak Toastmaster Chapter 2014  Took part in public speaking meeting every Friday and played different roles such as: Speaker, evaluator, quiz-master  Best Speaker Award winner for the club meeting

American Society for Cell Biology Annual Conference 2013  Attended the conference as a representative of James T. Blankenship Lab  Presented my project: “RalA Ragulates Furrow Formation During Early Drosophila Embryogenesis”

Association for the Advancement of Sustainability in High Education Conference 2010  Selected as the representative of the University of Denver  Gave a presentation about Damages of Mt Evans eco-system, Soil pollution of North Denver area, and Global Warming issues in Colorado

Publications

1. Ding X, Jo J, Wang CY, Cristobal CD, Zuo Z, Ye Q, Wirianto M, Lindeke-Myers A, Choi JM, Mohila CA, Kawabe H, Jun SY, Bellen HJ, Yoo SH, Lee HK (2020) The Daam2-VHL-Nedd4 Axis 1 Governs Developmental and Regenerative Oligodendrocyte Differentiation. Genes and Development :in press.

2. Martelli F, Zuo Z, Wang J, Wong CO, Karagas NE, Roessner U, Rupasinghe T, Venkatachalam K, Perry T, Bellen HJ, Batterham P (2020) Low doses of the neonicotinoid insecticide imidacloprid induce ROS triggering neurological and metabolic impairments in Drosophila. PNAS :In press.

3. Li X, Yang L, Mao Z, Pan X, Zhao Y, Gu X, Eckel-mahan K, Zuo Z, Tong Q, Hartig SM, Cheng X, Du G, Moore DD, Bellen HJ, Sesaki H, Sun K (2020) Novel role of dynamin-related-protein 1 in dynamics of ER-lipid droplets in adipose tissue. The FASEB Journal 34(6):8265-8282.

4. Chung HL, Wangler MF, Marcogliese PC, Jo J, Ravenscroft TA, Zuo Z, Duraine L, Sadeghzadeh S, Li-Kroeger D, Schmidt RE, Pestronk A, Rosenfeld JA, Burrage L, Herndon MJ, Chen S, Undiagnosed Diseases Network, Shillington A, Vawter-Lee M, Hopkin R, Rodriguez-Smith J, Henrickson M, Lee B, Moser AB, Jones RO, Watkins P, Yoo T, Mar S, Choi M, Bucelli RC, Yamamoto S, Lee HK, Prada CE, Chae JH, Vogel TP, Bellen HJ (2020) Loss or gain of function mutations in ACOX1 cause axonal loss via different mechanisms. Neuron 106:589-606.

5. Ye H, Ojelade SA, Li-Kroeger D, Zuo Z, Wang L,Y Li, Gu JYJ, Tepass U, Rodal AA, Bellen HJ, Shulman JM (2020) Retromer subunit, VPS29, regulates synaptic transmission and is required for endolysosomal function in the aging brain. eLife 9:e91577.

6. Cunningham K, Zhang K, Maulding K, Senturk M, Grima J, Sung H, Zuo Z, Song H, Rothstein J, Bellen HJ (2020) TFEB/Mitf links impaired nuclear import to autophagolysosomal dysfunction in C9-ALS. Nature Neuroscience submitted.

7. Wang J, Rousseau J, Kim E, Ehresmann S, Cheng YT, Duraine L, Zuo Z, Park YJ, Li-Kroeger D, Bi W, Wong LJ, Rosenfeld J, Gleeson J, Faqeih E, Alkuraya FS, Wierenga KJ, Chen J, Afenjar A, Nava C, Doummar D, Keren B, Juusola J, Grompe M, Bellen HJ, Campeau PM (2019) Loss of Oxidation Resistance 1, OXR1, is associated with an autosomal-recessive eurological disease with cerebellar atrophy and lysosomal dysfunction. American Journal of Human Genetics 105:1237- 1253.

8. Kanca O, Zirin J, Garcia-Marques J, Knight SM, Yang-Zhou D, Amador G, Chung HL, Zuo Z, Ma L, He Y, Lin WW, Fang Y, Ge M, Yamamoto S, Schulze KL, Hu Y, Spradling AC, Mohr SE, Perrimon N, Bellen HJ (2019) An efficient CRISPR-based strategy to insert small and large fragments of DNA using short homology arms. eLife 8:e51539.

9. Ojelade SA, Lee TV, Giagtzoglou N, Yu L, Ugur B, Li Y, Duraine L, Zuo Z, Petyuk V, De Jager PL, Bennett DS, Arenkiel BR, Bellen HJ, Shulman JM (2019) cindr, the Drosophila homolog of the CD2AP Alzheimer's disease susceptibility gene, is required for synaptic transmission and proteostasis. Cell Reports 28:1799-1813.

10. Brown AM, Arancillo M, Lin T, Catt DR, Zhou J, Lackey EP, Stay TL, Zuo Z, White JJ, Sillitoe RV. (2019) Molecular layer interneurons shape the spike activity of cerebellar Purkinje cells. Scientific Reports 9:1-19. 11. Mao D, Lin G, Tepe B, Zuo Z, Tan KL, Senturk M, Zhang S, Arenkiel BR, Sardiello M, Bellen HJ (2019) VAMP associated proteins are required for autophagic and lysosomal degradation by promoting a PtdIns4P-mediated endosomal pathway. Autophagy 11:1-20.

12. Li-Kroeger D, Kanca O, Lee PT, Cowan S, Lee MT, Jaiswal M, Salazar JL, He Y, Zuo Z, Bellen HJ (2018) An expanded toolkit for gene tagging based on MiMIC and scarless CRISPR tagging in Drosophila. eLife 7:e38709.

13. Marcogliese PC, Shashi V, Spillmann RC, Stong N, Rosenfeld JA, Koenig MK, Martínez-Agosto JA, Herzog M, Chen AH, Dickson PI, Lin HJ, Vera MU, Salamon N, Graham JM Jr, Ortiz D, Infante E, Steyaert W, Dermaut B, Poppe B, Chung HL, Zuo Z, Lee PT, Kanca O, Xia F, Yang Y, Smith EC, Jasien J, Kansagra S, Spiridigliozzi G, El-Dairi M, Lark R, Riley K, Koeberl DD, Golden-Grant K; Program for Undiagnosed Diseases (UD-PrOZA); Undiagnosed Diseases Network, Yamamoto S, Wangler MF, Mirzaa G, Hemelsoet D, Lee B, Nelson SF, Goldstein DB, Bellen HJ, Pena LDM (2018) IRF2BPL is associated with neurological phenotypes. American Journal of Human Genetics 103:245-260.

14. Lin G, Lee PT, Chen K, Mao D, Tan KL, Zuo Z, Lin WW, Wang L, Bellen HJ (2018) Phospholipase PLA2G6, a Parkinsonism-associated gene, affects Vps26 and Vps35, retromer function, and ceramide levels, similar to α-Synuclein gain. Cell Metabolism 28:605-618.

15. Lee PT, Zirin J, Kanca O, Lin WW, Schulze KL, Li-Kroeger D, Tao R, Devereaux C, Hu Y, Chung V, Fang Y, He Y, Pan H, Ge M, Zuo Z, Housden BE, Mohr SE, Yamamoto S, Levis RW, Spradling AC, Perrimon N, Bellen HJ (2018) A gene-specific T2A-GAL4 library for Drosophila. eLife 7:e35574.

16. Ugur B, Bao H, Stawarski M, Duraine LR, Zuo Z, Lin YQ, Neely GG, Macleod GT, Chapman ER, Bellen HJ (2017) The Krebs cycle enzyme Isocitrate Dehydrogenase 3A couples mitochondrial metabolism to synaptic transmission. Cell Reports 21:3794-3806.

17. Luo X, Rosenfeld JA, Yamamoto S, Harel T, Zuo Z, Hall M, Wierenga KJ, Pastore MT, Bartholomew D, Delgado MR, Rotenberg J, Lewis RA, Emrick L, Bacino CA, Eldomery MK, Coban Akdemir Z, Xia F, Yang Y, Lalani SR, Lotze T, Lupski JR, Lee B, Bellen HJ, Wangler MF; Members of the UDN (2017) Clinically severe CACNA1A alleles affect synaptic function and neurodegeneration differentially. PLoS Genetics 13:e1006905.

18. Nagarkar-Jaiswal S, Manivannan SN, Zuo Z, Bellen HJ (2017) A cell cycle-independent, conditional gene inactivation strategy for differentially tagging wild-type and mutant cells. eLife 6:e26420.

19. Chouhan AK, Guo C, Hsieh YC, Ye H, Senturk M, Zuo Z, Li Y, Chatterjee S, Botas J, Jackson GR, Bellen HJ, Shulman JM (2016) Uncoupling neuronal death and dysfunction in Drosophila models of neurodegenerative disease. Acta Neuropathologica Communications 4:62.

20. Mavor LM, Miao H, Zuo Z, Holly RM, Xie Y, Loerke D, Blankenship JT (2016) Rab8 directs furrow ingression and membrane addition during epithelial formation in Drosophila melanogaster. Development 143:892-903.

21. David-Morrison G, Xu Z, Rui YN, Charng WL, Jaiswal M, Yamamoto S, Xiong B, Zhang K, Sandoval H, Duraine L, Zuo Z, Zhang S, Bellen HJ (2016) WAC regulates mTOR activity by acting as an adaptor for the TTT and Pontin/Reptin complexes. Developmental Cell 36:139-151.

22. Nagarkar-Jaiswal S, DeLuca SZ, Lee PT, Lin WW, Pan H, Zuo Z, Lv J, Spradling AC, Bellen HJ (2015) A genetic toolkit for tagging intronic MiMIC containing genes. eLife 4:e08469.

23. Holly RM, Mavor LM, Zuo Z, Blankenship JT (2015) A rapid, membrane-dependent pathway directs furrow formation through RalA in the early Drosophila embryo. Development 142:2316- 2328.