The Microbial Glycobiology Conference Draft Program

Organizers:

Miguel A. Valvano, MD Nathalie Juge, PhD Nichollas E. Scott, Fikri Avci, PhD. Queen's University Quadram Institute PhD. University of University of Georgia, Belfast, UK Biosciences, UK Melbourne, Australia USA

======Information to speakers, poster presenters and attendees

Keynote lecture: 25 min, 5 min for questions; additional question online follow-up (recommended 20 slides)

Invited speakers Talks: 15 min, 5 min for questions; additional question online follow-up (recommended 10 slides).

Meeting's Library: Speakers' talks not time-scheduled in the programe to be accessed by the attendants in their own time.

Short talks from Abstracts: 8 min, 2 min questions; additional question online follow-up (3-4 slides)

Poster Sessions: posters will be available online 2 days prior to 1.5-h virtual meeting; session time will be devoted to live chat among attendees

Q&A: Live Q&A after scheduled talks and more Q&A via chat, providing excellent opportunities to interact with speakers and poster presenters.

Many talks will be pre-recorded to allow for posting during a few days, so registered attendees from different time zones will be able to see the talks in their own time. ======July 13, 2020 - Day 1 (Please note that all session times are based on British Summer Time – BST)

10:00 - 10:15 Introductory Remarks Miguel A. Valvano (Queen's University Belfast, UK)

Session 1: Microbial Glycobiology in the Gut Session Chair: Nathalie Juge (Quadram Institue Biosciences, UK)

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10:15 - 10:35 Different Levels of Intestinal Protection Against Gunnar Hanson (University of Gothenburg, Sweden)

10:35 - 10:55 Transition of the Gut Microbiome from a Milk to a Mucosal Environment Clara Belzer (Wageningen University, The Netherlands)

10:55 - 11:15 Glycans in the Regulation of Microbial Virulence Katharina Ribbeck (Massachusetts Institute of Technology, USA)

11:15 - 11:45 Short talks

Uncovering the Molecular Mechanism of Sialic Acid Derivative Catabolism Across Bacterial Species Andrew Bell (Quadram Institute Bioscience, UK)

Prominent Members of the Human Gut Microbiota Express Endo-acting O- glycanases to Initiate Mucin Breakdown Lucy I. Crouch (University of Birmingham, UK)

Xanthan Gum Digestion by Human Gut Microbiota Matthew P. Ostrowski (University of Michigan, USA)

11:45 - 12:05 Surface Glycan-binding Proteins Drive Carbohydrate Foraging by the Human Gut Bacteroidetes Nicole Koropatkin (University of Michigan, USA)

12:05 - 12:25 Glycans on the Menu: bacterial Consumption of Sialic Acids Gavin Thomas (University of York, UK)

12:25 - 13:25 Break/Meet the Expert Session Webinar: Nicole Korpatkin, Gunnar Hansson, Nichollas Scott, Justin Boddey

Meeting's Library: Molecular Insight into Dietary Beta-glucan Utilization by Individual Members of the Human gut Microbiota Harry Brumer (University of British Columbia, Canada)

Mucus Associated Bacteria in the Gut: The Case of Ruminococcus gnavus Nathalie Juge (Quadram Institute Biosciences, UK)

Keynote Lecture 13:25 - 13:55 M. Stephen Trent (University of Georgia, USA A) Session Chair: Miguel Valvano

Session 2: Virulence, Prophylactics and Therapeutics Session Chair: Fikri Avci (University of Georgia, USA)

13:55 - 14:15 Synthetic Lipid A and Lipid A Mimetics as Tools for Studying LPS – Protein Interactions 2

Alla Zamyatina (BOKU, Austria)

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14:15 - 14:35 Bacterial Capsule polymerases: Tools for the Production of Nature-identical Vaccines Rita Gerardy-Schahn (Hannover Medical School, Germany)

14:35 - 14:55 Sweet Revenge - Bacterial Immune Evasion Through Specific Antibody Glycan Hydrolysis Mattias Collin (University of Lund, Sweeden)

14:55 - 15:10 Break

15:10 - 15:30 Immune Mechanisms Induced by Viral Surface Glycoproteins Fikri Avci (University of Georgia, USA)

15:30 - 16:30 Short talks

Sulfatases and Fucosidases Play Critical Roles in Mucin Utilization by Bacteroides Ana S. Luis (Gothenburg University, Sweden)

A Novel Sialic Acid-binding Adhesin Present in Multiple Species Contributes to the Pathogenesis of Infective Endocarditis Meztlli O. Gaytán (Abigail Wexner Research Institute at Nationwide Children's Hospital, Columbus, Ohio, USA)

Microbial Degradation of Ileal Mucus Promotes Rotavirus Infection Mindy A. Engevik (Baylor College of Medicine, Texas, USA)

A Cell-Based Mucin Display for Dissection of Microbial Interactions at the Mucosa Christian Büll (Copenhagen Center for Glycomics, Denmark)

New Insights into the Biochemical Function of a Methylesterase Essential for Pectin Metabolism in the Human Gut Microbe Bacteroides thetaiotaomicron Didier Ndeh (Quadram Institute Bioscience, UK)

Type 9 Secretion of Glycoside Hydrolase Enzymes in a Gut Bacteroides Enables ‘Sharing’ During Glycan Metabolism Ekaterina Buzun (Newcastle University, UK)

Meeting's Library: Insight into the immune response arising from mycobacterial infection facilitated by a glycan array Todd Lowary (University of Alberta, Canada)

Poster Session #1 16:00 - 17:30 Posters will be available online 1-2 days prior to the virtual meeting; session time will be devoted to live chat among attendees) - (Exhibit) 4

July 14, 2020 - Day 2

Session 3: Microbial Glycan Synthesis and Surfaces I Session Chair: TBA

10:00 - 10:20 C- and O-glycosylation Ensures Protein Quality Control Essential for Plasmodium falciparum Transmission and Virulence Justin Boddey (Walter & Eliza Hall Institute of Medical Research, Australia)

10:20 - 10:40 How Many Proteins are Glycosylated in Burkholderia Species? Nichollas Scott (University of Melbourne, Australia)

10:40 - 11:00 Semi-synthesis of Novel Probes to Study Bacterial Glycolipid-processing Enzyme Stephen Cochrane (Queen's University Belfast, UK)

11:00 - 11:20 O-linked Protein Glycosylation in the Genus Neisseria: Coordinated Evolution of Glycoforms and Protein Substrate Targeting J. Michael Koomey (University of Oslo)

11:20 - 11:40 Functional and Biochemical Characterization of SPY- catalysed Nucleocytosolic O-fucosylation in Toxoplasma Gondii Giulia Bandini (University of York, UK)

11:40 - 12:10 Short talks

High-resolution Mapping of Glycoprotein Structure–activity Relationships by Shotgun Scanning Glycomutagenesis Mingji Li (Cornell University, NY, USA)

Influence of Single Deletion of N-glycosylation Pathway Genes on Recombinant Enzymes Production/secretion Jaqueline A. Gerhardt (University of Campinas, Brazil)

The Structure of GalNAc-T12 Reveals How Non-conserved Residues Dictate its Function Nadine L. Samara (National Institutes of Health, Bethesda, USA)

On Demand Meeting's Library: Pathogen Arginine Glycosyltransferases Targeting the Host Elizabeth Hartland (Hudson Institute, Australia)

α-Gal Glycotopes as Vaccines and Therapeutic Biomarkers for Chagas Disease Igor Almeida (University of Texas, USA)

12:10 - 13:10 Break/Meet the Expert Session Webinar: 5

Stephen Trent, Zuzanna Drullis-Kawaa, Fikri Avci, Carmen Galan

Session 4: Microbial glycan synthesis and Surfaces II Session Chair: TBA

13:10 - 13:30 Phages and Phage-borne Glycan Degrading Enzymes as Alternative Antimicrobials Zuzanna Drullis-Kawa (Wroclaw University, Poland)

13:30 - 13:50 Antibacterial Green Fluorescent Amine Coated Carbon Nanodots (CDs) as Potential Labelling Probes for Clinical Microorganisms M. Carmen Galan (University of Bristol, UK)

13:50 - 14:10 Identifying the Lipid A Adaptations to Virulence Jose A. Bengoechea (Queen's University Belfast, UK)

14:10 - 14:30 Novel Control Mechanisms for LPS Transport by the Lpt Machinery Revealed by Lpt Mutant Analysis Paola Sperandeo (University of Milan, Italy)

14:30 - 14:45 Break

14:45 - 15:05 Making a Dent in Campylobacteriosis by Biting in Campylobacter jejuni’s Sugar Coat Carole Creuzenet (Western University, Canada)

15:05 - 16:05 Short talks

O-acetylation Controls Glycosylation on the Serine-rich Repeat Protein GspB, Ensuring Optimal Streptococcal Binding to Human Platelets Ravin Seepersaud (San Francisco Veteran Affairs Medical Center & the University of California San Francisco, USA)

Insights into the Role of GtfCs in O-protein Glycosylation Pathways of Gut Commensal Bacteria Dimitris Latousakis (Quadram Institue Biosciences, UK)

Harnessing Bacteriophages to Understand and Modify the Listeria Surface Yang Shen (Institute of Food, Nutrition and Health, ETH, Switzerland)

How do Bacteria Change Their Coats? Structural Analysis of Acyltransferases Involved in O-antigen Modification Sarah N. Tindall (University of York, UK)

Siglec-7 Contributes to the Immunomodulation Induced by F. nucleatum ssp. in Colorectal Cancer Dimitra Lamprinaki (Quadram Institute Bioscience, UK)

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Pseudomonas aeruginosa Display Carbohydrate Ligands for C- type Lectin Receptors That Interfere With Their Receptor Function Kelly Lee (University of Nottingham, UK)

Wrap-up 16:05 - 16:20 Theatre/Main Stage

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