Product datasheet Anti-GJA4 Antibody Catalog Number: BA3314

BOSTER BIOLOGICAL TECHNOLOGY Special NO.1, International Enterprise Center, 2nd Guanshan Road, Wuhan, China

Web: www.boster.com.cn Phone: +86 27 67845390 Fax: +86 27 67845390 Email: [email protected]

Basic Information Product Name Anti-GJA4 Antibody Name GJA4 Source Rabbit IgG Species Reactivity human,mouse,rat Tested Application WB,IHC-P,ICC Contents 500ug/ml antibody with PBS ，0.02% NaN3 , 1mg BSA and 50% glycerol. Immunogen A synthetic peptide corresponding to a sequence at the N-terminus of human 37(3-17aa DWGFLEKLLDQVQEH), identical to the related mouse and rat sequences. Purification Immunogen affinity purified. Observed MW 37KD Dilution Ratios Western blot: 1:500-2000 Immunohistochemistry(Paraffin-embedded Section): 1:50-400 Immunocytochemistry: 1:50-400 (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user.

Storage 12 months from date of receipt，-20℃ as supplied.6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing

Background Information alpha-4 (GJA4), also called CX37 is a protein that in humans is encoded by the GJA4 gene. This gene is mapped to 1p34.3. This gene encodes a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. Mutations in this gene have been associated with atherosclerosis and a higher risk of myocardial infarction. CX37 had been shown to reflect the stage and maturity of luteinized follicles in animal studies.

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FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE. 1 Product datasheet Anti-GJA4 Antibody Catalog Number: BA3314

BOSTER BIOLOGICAL TECHNOLOGY Special NO.1, International Enterprise Center, 2nd Guanshan Road, Wuhan, China

Web: www.boster.com.cn Phone: +86 27 67845390 Fax: +86 27 67845390 Email: [email protected]

Selected Validation Data

Figure 1. Western blot Figure 2. IHC analysis of analysis of Connexin Connexin 37/GJA4 using 37/GJA4 using anti- anti-Connexin 37/GJA4 Connexin 37/GJA4 antibody (BA3314). antibody (BA3314). Connexin 37/GJA4 was Electrophoresis was detected in frozen performed on a 10% SDS- section of human PAGE gel at 70V intestinal cancer tissues. (Stacking gel) / 90V The tissue section was (Resolving gel) for 2-3 blocked with 10% goat hours. The sample well of serum. The tissue section each lane was loaded was then incubated with with 50ug of sample 1μg/ml rabbit anti- under reducing Connexin 37/GJA4 conditions. Lane 1: Antibody (BA3314) mouse liver tissue lysates, overnight at 4°C. Lane 2: rat liver tissue Biotinylated goat anti- lysates,Lane 3: mouse rabbit IgG was used as spleen tissue lysates,Lane secondary antibody and 4: mouse brain tissue incubated for 30 minutes lysates,Lane 5; mouse at 37°C. The tissue HEPA1-6 whole cell section was developed lysates,Lane 6: mouse using Strepavidin-Biotin- NIH/3T3 whole cell Complex (SABC)(Catalog lysates,Lane 7: human # SA1022) with DAB as PANC-1 whole cell the chromogen. lysates,Lane 8: human THP-1 whole cell lysates.After Electrophoresis, were transferred to a Nitrocellulose membrane at 150mA for 60 minutes. Blocked the membrane with 5% Non-fat Milk in TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti- Connexin 37/GJA4 antigen affinity purified polyclonal antibody (Catalog # BA3314) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.05%Tween 3 times with 5 minutes each and probed with a goat anti- rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE. 2 Product datasheet Anti-GJA4 Antibody Catalog Number: BA3314

BOSTER BIOLOGICAL TECHNOLOGY Special NO.1, International Enterprise Center, 2nd Guanshan Road, Wuhan, China

Web: www.boster.com.cn Phone: +86 27 67845390 Fax: +86 27 67845390 Email: [email protected]

hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Connexin 37/GJA4 at approximately 35-37KD. The expected band size for Connexin 37/GJA4 is at 37KD.

Figure 3. IHC analysis of Figure 4. IHC analysis of Connexin 37/GJA4 using Connexin 37/GJA4 using anti-Connexin 37/GJA4 anti-Connexin 37/GJA4 antibody (BA3314). antibody (BA3314). Connexin 37/GJA4 was Connexin 37/GJA4 was detected in frozen detected in section of Rat Cardiac immunocytochemical Muscle tissues. The tissue section of HELA cell. section was blocked with Enzyme antigen retrieval 10% goat serum. The was performed using IHC tissue section was then enzyme antigen retrieval incubated with 1μg/ml reagent (AR0022) for 15 rabbit anti-Connexin mins. The cells were 37/GJA4 Antibody blocked with 10% goat (BA3314) overnight at serum. And then 4°C. Biotinylated goat incubated with 1μg/ml anti-rabbit IgG was used rabbit anti-Connexin as secondary antibody 37/GJA4 Antibody and incubated for 30 (BA3314) overnight at minutes at 37°C. The 4°C. Biotinylated goat tissue section was anti-rabbit IgG was used developed using Strepavi as secondary antibody din-Biotin-Complex and incubated for 30 (SABC)(Catalog # minutes at 37°C. The SA1022) with DAB as the section was developed chromogen. using Strepavidin-Biotin- Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE. 3

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