FEMS Microbiology Ecology, 96, 2020, fiaa087 doi: 10.1093/femsec/fiaa087 Advance Access Publication Date: 9 May 2020 Research Article Downloaded from https://academic.oup.com/femsec/article/96/8/fiaa087/5835220 by Leiden University / LUMC user on 02 June 2021 RESEARCH ARTICLE Global warming shifts the composition of the abundant bacterial phyllosphere microbiota as indicated by a cultivation-dependent and -independent study of the grassland phyllosphere of a long-term warming field experiment Ebru L. Aydogan1,†, Olga Budich1,†, Martin Hardt2, Young Hae Choi3, Anne B. Jansen-Willems4, Gerald Moser4, Christoph Muller¨ 4,5, Peter Kampfer¨ 1 and Stefanie P. Glaeser1,*,‡ 1Institute of Applied Microbiology (IFZ), Justus Liebig University Giessen, D-35392 Giessen, Germany, 2Biomedical Research Center Seltersberg – Imaging Unit, Justus Liebig University Giessen, D-35392 Giessen, Germany, 3Natural Products Laboratory, Institute of Biology, Leiden University, Sylviusweg 72, 2333 BE Leiden, The Netherlands, 4Institute of Plant Ecology (IFZ), Justus Liebig University Giessen, D-39392 Giessen, Germany and 5School of Biology and Environmental Science and Earth Institute, University College Dublin, Belfield, D04V1W8 Dublin, Ireland ∗Corresponding author: Institute of Applied Microbiology (IFZ), Justus Liebig University Giessen, IFZ-Heinrich-Buff-Ring 26–32, D-35392 Giessen, Germany. E-mail:
[email protected] One sentence summary: Global warming shifts the phyllosphere microbiota. †These are shared first authors. Editor: Angela Sessitsch ‡Stefanie P. Glaeser, http://orcid.org/0000-0001-5258-6195 ABSTRACT The leaf-colonizing bacterial microbiota was studied in a long-term warming experiment on a permanent grassland, which had been continuously exposed to increased surface temperature (+2◦C) for more than six years.