TCF3 Antibody Cat. No.: 5609
TCF3 Antibody
Immunohistochemistry of TCF3 in rat liver tissue with Immunofluorescence of TCF3 in rat brain tissue with TCF3 TCF3 antibody at 5 μg/mL. antibody at 20 μg/mL.
Specifications
HOST SPECIES: Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
HOMOLOGY: Predicted species reactivity based on immunogen sequence: Chicken: (86%)
TCF3 antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human TCF3. IMMUNOGEN:
The immunogen is located within amino acids 150 - 200 of TCF3.
TESTED APPLICATIONS: ELISA, IF, IHC-P, WB
October 1, 2021 1 https://www.prosci-inc.com/tcf3-antibody-5609.html TCF3 antibody can be used for detection of TCF3 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL. APPLICATIONS: Antibody validated: Western Blot in human samples; Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.
POSITIVE CONTROL: 1) Cat. No. 1303 - Human Brain Tissue Lysate
Properties
PURIFICATION: TCF3 Antibody is affinity chromatography purified via peptide column.
CLONALITY: Polyclonal
ISOTYPE: IgG
CONJUGATE: Unconjugated
PHYSICAL STATE: Liquid
BUFFER: TCF3 Antibody is supplied in PBS containing 0.02% sodium azide.
CONCENTRATION: 1 mg/mL
TCF3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. STORAGE CONDITIONS: As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Additional Info
OFFICIAL SYMBOL: TCF3
TCF3 Antibody: E2A, E47, ITF1, VDIR, TCF-3, bHLHb21, BHLHB21, E2A, Transcription factor ALTERNATE NAMES: E2-alpha, Class B basic helix-loop-helix protein 21
ACCESSION NO.: NP_003191
PROTEIN GI NO.: 27777636
GENE ID: 6929
USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background and References
October 1, 2021 2 https://www.prosci-inc.com/tcf3-antibody-5609.html TCF3 Antibody: The TCF3 gene, also called E2A, encodes two basic helix-loop-helix (bHLH) transcription factors, E12 and E47, through alternative splicing. These transcription factors are involved in mediating canonical Wnt signaling, which is very important in a diverse array of cellular functions such as stem cell proliferation, self-renewal, activation, fate BACKGROUND: determination, differentiation and aging and senescence. They bind beta-catenin and can act as transcriptional activators or repressors for Wnt target genes, and have been shown to regulate specific target genes during CNS development downstream of Wnt signaling. TCF3/Lef complexes are also known to play key roles in controlling cell fate lineages in multipotent skin stem cells.
1) Korinek V, Barker N, Willert K, et al. Two members of the Tcf family implicated in REFERENCES: Wnt/beta-catenin signaling during embryogenesis in the mouse. Mol. Cell Biol.1998; 18:1248-1256.
2) Gribble SL, Kim HS, Bonner J, et al. Tcf3 inhibits spinal cord neurogenesis by regulating sox4a expression. Dev. Cell2009; 136:781-9.
3) Cole MF, Johnstone SE, Newman JJ, et al. Tcf3 is an integral component of the core regulatory circuitry of embryonic stem cells. Genes Dev.2008;22:746-55.
4) Nguyen H, Rendl M and Fuchs E. Tcf3 governs stem cell features and represses cell fate determination in skin. Cell2006; 127:171-83.
ANTIBODIES FOR RESEARCH USE ONLY.
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